Construct: sgRNA BRDN0001145795
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CAAAAAGTAAGCTCCTAAGG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- IP6K2 (51447)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77817
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000003.12 |
3 |
48693049 |
- |
IP6K2 |
NNNAAAGTAAGCTCCTAAGG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000009.12 |
9 |
103004496 |
+ |
CYLC2 |
NNNAAAGTAAGCTCCAAATG |
NGG |
2 |
0.6061 |
Tier II |
| 3 |
NC_000003.12 |
3 |
184377435 |
- |
THPO |
NNNAAGGTAAGCCCCTAAGG |
NGG |
2 |
0.5639 |
Tier II |
| 4 |
NC_000010.11 |
10 |
72764070 |
- |
MCU |
NNNAAAGTCAGCTCCTAAGA |
NGG |
2 |
0.5357 |
Tier II |
| 5 |
NC_000006.12 |
6 |
36419982 |
+ |
PXT1 |
NNNAGAGTAAGCTCCTAAGT |
NGG |
2 |
0.504 |
Tier II |
| 6 |
NC_000014.9 |
14 |
79282197 |
+ |
NRXN3 |
NNNAAACTTAGCTCCTAAGG |
NGG |
2 |
0.4125 |
Tier II |
| 7 |
NC_000001.11 |
1 |
33138230 |
+ |
AZIN2 |
NNNAAAGTAGGCTTCTAAGG |
NGG |
2 |
0.4074 |
Tier II |
| 8 |
NC_000001.11 |
1 |
33138230 |
+ |
ZNF362 |
NNNAAAGTAGGCTTCTAAGG |
NGG |
2 |
0.4074 |
Tier II |
| 9 |
NC_000008.11 |
8 |
6743767 |
+ |
AGPAT5 |
NNNAAAGTAAGCCCCTATGG |
NGG |
2 |
0.3947 |
Tier II |
| 10 |
NC_000004.12 |
4 |
146794670 |
- |
TTC29 |
NNNATAGTAAGCTCCTAAGG |
NGG |
1 |
0.3636 |
Tier II |
| 11 |
NC_000001.11 |
1 |
8738779 |
- |
RERE |
NNNAAAGGAACCTCCTAAGG |
NGG |
2 |
0.3143 |
Tier II |
| 12 |
NC_000011.10 |
11 |
47325219 |
- |
MADD |
NNNAAAGTCAGCTCCTATGG |
NGG |
2 |
0.2857 |
Tier II |
| 13 |
NC_000006.12 |
6 |
108629006 |
- |
FOXO3 |
NNNCCAGTAAGCTCCTAAGG |
NGG |
2 |
0.1765 |
Tier II |
| 14 |
NC_000009.12 |
9 |
17988895 |
- |
ADAMTSL1 |
NNNAAAATAAGCTCCTTAGG |
NGG |
2 |
0.1333 |
Tier II |
| 15 |
NC_000001.11 |
1 |
112435356 |
+ |
CTTNBP2NL |
NNNAATGTAAGCTCCTCAGG |
NGG |
2 |
0.1261 |
Tier II |
| 16 |
NC_000011.10 |
11 |
70503246 |
- |
SHANK2 |
NNNAAAGTAAGCACCTAAGG |
NTG |
2 |
0.027 |
Tier II |
| 17 |
NC_000011.10 |
11 |
21324052 |
+ |
NELL1 |
NNNATAGTAAGCTCCTAAGG |
NGA |
2 |
0.0253 |
Tier II |
| 18 |
NC_000008.11 |
8 |
96336225 |
+ |
PTDSS1 |
NNNACAGTAAGCTCCTAAGG |
NGC |
2 |
0.0111 |
Tier II |
| 19 |
NC_000010.11 |
10 |
72764070 |
- |
LOC124902450 |
NNNAAAGTCAGCTCCTAAGA |
NGG |
2 |
0.5357 |
Tier III |
| 20 |
NC_000002.12 |
2 |
220511653 |
+ |
LOC105373894 |
NNNAAAGTCTGCTCCTAAGG |
NGG |
2 |
0.5042 |
Tier III |
| 21 |
NC_000015.10 |
15 |
39075877 |
+ |
LOC105370777 |
NNNAAAGTGAGCTCCTAATG |
NGG |
2 |
0.4444 |
Tier III |
| 22 |
NC_000005.10 |
5 |
73145284 |
- |
LOC105379030 |
NNNAAACTCAGCTCCTAAGG |
NGG |
2 |
0.3929 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000068.7 |
2 |
130279003 |
+ |
Nop56 |
NNNAAAGAAAGCTCCCAAGG |
NGG |
2 |
0.5333 |
Tier I |
| 2 |
NC_000075.6 |
9 |
108798402 |
+ |
Ip6k2 |
NNNAAAGTAAGCTGCTAAGA |
NGG |
2 |
0.0 |
Tier I |
| 3 |
NC_000071.6 |
5 |
68084837 |
+ |
Grxcr1 |
NNNAAAGTTAGCTCCTAAGG |
NGG |
1 |
0.6 |
Tier II |
| 4 |
NC_000078.6 |
12 |
117902469 |
- |
Dnah11 |
NNNAACGTAAGCTCCTAAGG |
NGG |
1 |
0.4545 |
Tier II |
| 5 |
NC_000077.6 |
11 |
76112646 |
+ |
Vps53 |
NNNAAAGTGGGCTCCTAAGG |
NGG |
2 |
0.3704 |
Tier II |
| 6 |
NC_000083.6 |
17 |
48304553 |
+ |
Treml2 |
NNNAAAGCAAGCTCCTTAGG |
NGG |
2 |
0.1333 |
Tier II |
| 7 |
NC_000081.6 |
15 |
48046017 |
+ |
Csmd3 |
NNNATAGTAAGCTACTAAGG |
NGG |
2 |
0.1273 |
Tier II |
| 8 |
NC_000072.6 |
6 |
23840836 |
+ |
Cadps2 |
NNNAAAGTAACCTCCTAAGG |
NAG |
2 |
0.1111 |
Tier II |
| 9 |
NC_000077.6 |
11 |
3349085 |
- |
Limk2 |
NNNAAAGTAAGCTCCTAAGA |
NTG |
2 |
0.0365 |
Tier II |
| 10 |
NC_000069.6 |
3 |
127162626 |
- |
Ank2 |
NNNAAAGCAAGCTGCTAAGG |
NGG |
2 |
0.0 |
Tier II |
| 11 |
NC_000068.7 |
2 |
130120575 |
- |
Tgm6 |
NNNAAAGTAAGGTGCTAAGG |
NGG |
2 |
0.0 |
Tier II |
| 12 |
NC_000074.6 |
8 |
10606929 |
+ |
Myo16 |
NNNAACGTAAGCTGCTAAGG |
NGG |
2 |
0.0 |
Tier II |
| 13 |
NC_000075.6 |
9 |
36813346 |
- |
Gm27219 |
NNNAAAGAAAGCTCCCAAGG |
NGG |
2 |
0.5333 |
Tier III |
| 14 |
NC_000085.6 |
19 |
5436508 |
+ |
Gm25432 |
NNNAAAGAAAGCTCCCAAGG |
NGG |
2 |
0.5333 |
Tier III |
| 15 |
NC_000086.7 |
X |
12619698 |
- |
Gm9432 |
NNNAAAGAAAGCTCCCAAGG |
NGG |
2 |
0.5333 |
Tier III |
| 16 |
NC_000082.6 |
16 |
86251261 |
- |
Gm32357 |
NNNAAAGGAAGCTCCCAAGG |
NGG |
2 |
0.4889 |
Tier III |
| 17 |
NC_000072.6 |
6 |
68530050 |
+ |
Igk |
NNNAAAGTAAGCCCTTAAGG |
NGG |
2 |
0.0526 |
Tier III |
| 18 |
NC_000078.6 |
12 |
27062757 |
- |
Gm30515 |
NNNAAAGAAAGCTCCTAAGG |
NGT |
2 |
0.0129 |
Tier III |
| 19 |
NC_000069.6 |
3 |
127162626 |
- |
Gm16958 |
NNNAAAGCAAGCTGCTAAGG |
NGG |
2 |
0.0 |
Tier III |
Other clones with same target sequence:
(none)
