Construct: sgRNA BRDN0001145812

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
AAGAGTAAAGCAATGGCCAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ROR1 (4919)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76045

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000001.11 1 64159055 - ROR1 NNNAGTAAAGCAATGGCCAG NGG 0 1.0 Tier I
2 NC_000008.11 8 131984286 - EFR3A NNNTGTAAAGCAATGGCCAG NCG 2 0.0682 Tier I
3 NC_000017.11 17 78750024 + CYTH1 NNNAGCAAATCAATGGCCAG NGG 2 0.7386 Tier II
4 NC_000001.11 1 51914302 + RAB3B NNNTGTAAAGCAATGGCCAG NGG 1 0.6364 Tier II
5 NC_000010.11 10 29689943 + SVIL NNNGGTAAAACAATGGCCAG NGG 2 0.5833 Tier II
6 NC_000009.12 9 91451219 - NFIL3 NNNAGTAAAACAAAGGCCAG NGG 2 0.5778 Tier II
7 NC_000016.10 16 50292084 - ADCY7 NNNAGCAAAGCAAAGGCCAG NGG 2 0.5628 Tier II
8 NC_000012.12 12 29437670 + OVCH1 NNNAGTAAAGAGATGGCCAG NGG 2 0.5417 Tier II
9 NC_000004.12 4 72453118 - ADAMTS3 NNNAATAAAGCAATGGCCTG NGG 2 0.4667 Tier II
10 NC_000011.10 11 22687807 + GAS2 NNNAGTAAAGCAATGAACAG NGG 2 0.4667 Tier II
11 NC_000003.12 3 8741624 + CAV3 NNNAGTAACTCAATGGCCAG NGG 2 0.4643 Tier II
12 NC_000003.12 3 8741624 + OXTR NNNAGTAACTCAATGGCCAG NGG 2 0.4643 Tier II
13 NC_000002.12 2 235570140 - AGAP1 NNNAGTAAAGCAAAGGCTAG NGG 2 0.398 Tier II
14 NC_000014.9 14 94175504 - PPP4R4 NNNAATTAAGCAATGGCCAG NGG 2 0.3792 Tier II
15 NC_000008.11 8 98363863 - STK3 NNNACTATAGCAATGGCCAG NGG 2 0.3367 Tier II
16 NC_000001.11 1 109335969 - SORT1 NNNATTAAAACAATGGCCAG NGG 2 0.28 Tier II
17 NC_000011.10 11 78983027 - TENM4 NNNAGTCAAGCAAAGGCCAG NGG 2 0.2708 Tier II
18 NC_000006.12 6 64032936 + EYS NNNAGTAAATCATTGGCCAG NGG 2 0.2438 Tier II
19 NC_000001.11 1 9741592 - CLSTN1 NNNAGAAAAGGAATGGCCAG NGG 2 0.2167 Tier II
20 NC_000007.14 7 18820625 + HDAC9 NNNAGTAGAGGAATGGCCAG NGG 2 0.1833 Tier II
21 NC_000022.11 22 37145409 - IL2RB NNNAATAAAGCACTGGCCAG NGG 2 0.1825 Tier II
22 NC_000003.12 3 108505204 - MYH15 NNNAGTAAAACAATTGCCAG NGG 2 0.1333 Tier II
23 NC_000012.12 12 66159694 - TMBIM4 NNNAGTTAAGGAATGGCCAG NGG 2 0.1094 Tier II
24 NC_000011.10 11 1919558 - TNNT3 NNNAGTATAGGAATGGCCAG NGG 2 0.1071 Tier II
25 NC_000001.11 1 179612304 - TDRD5 NNNAATAAAGCAATGGGCAG NGG 2 0.051 Tier II
26 NC_000007.14 7 32529588 + AVL9 NNNAGTTAAGCAATGGCCAG NGA 2 0.0304 Tier II
27 NC_000002.12 2 114707778 + DPP10 NNNAGTAGAGCAATGGCCAG NGT 2 0.0118 Tier II
28 NC_000001.11 1 116766922 + CD2 NNNAGTAAACCAATGGCCAG NGC 2 0.0089 Tier II
29 NC_000006.12 6 125855367 - NCOA7 NNNTGTAAAGCAAGGGCCAG NGG 2 0.0 Tier II
30 NC_000012.12 12 29437670 + OVCH1-AS1 NNNAGTAAAGAGATGGCCAG NGG 2 0.5417 Tier III
31 NC_000013.11 13 34521029 - LINC00457 NNNAGTAAAGCAATGGTCAG NGG 1 0.4667 Tier III
32 NC_000013.11 13 34521029 - LINC02343 NNNAGTAAAGCAATGGTCAG NGG 1 0.4667 Tier III
33 NC_000001.11 1 152241740 - CCDST NNNAGTAAAACAATGGACAG NGG 2 0.4356 Tier III
34 NC_000009.12 9 93338493 - FAM120A2P NNNTGTAAAGCAATGGCCTG NGG 2 0.3427 Tier III
35 NC_000006.12 6 64032936 + LOC107986608 NNNAGTAAATCATTGGCCAG NGG 2 0.2438 Tier III
36 NC_000006.12 6 18535157 - MIR548A1HG NNNAGTACAGCAATGGCAAG NGG 2 0.2308 Tier III
37 NC_000001.11 1 19006653 + LOC105376815 NNNAGTAAAGGAATGCCCAG NGG 2 0.0 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000073.6 7 113316704 - Btbd10 NNNAGTAAAGAAAAGGCCAG NGG 2 0.4643 Tier I
2 NC_000081.6 15 65854776 - Efr3a NNNTGTAAAGCAATGGCCAG NCG 2 0.0682 Tier I
3 NC_000076.6 10 21309798 - Hbs1l NNNAGTAAAGCACTGGCCAG NGC 2 0.0047 Tier I
4 NC_000085.6 19 53158832 - Add3 NNNAATAAAGCAATGGCCAG NGG 1 0.8667 Tier II
5 NC_000067.6 1 187360492 + Gpatch2 NNNAGAGAAGCAATGGCCAG NGG 2 0.6118 Tier II
6 NC_000068.7 2 26891417 + Surf6 NNNAGTAAAGCAAAAGCCAG NGG 2 0.5826 Tier II
7 NC_000077.6 11 75941111 + Rph3al NNNAGCAAAGCAAAGGCCAG NGG 2 0.5628 Tier II
8 NC_000080.6 14 119808157 - Hs6st3 NNNACTAAAGCAGTGGCCAG NGG 2 0.5124 Tier II
9 NC_000085.6 19 42965619 + Hpse2 NNNAGTAAATCAAAGGCCAG NGG 2 0.503 Tier II
10 NC_000086.7 X 73813541 + Pdzd4 NNNAGTAAGGCAAAGGCCAG NGG 2 0.4127 Tier II
11 NC_000078.6 12 110919716 - Tecpr2 NNNAGCACAGCAATGGCCAG NGG 2 0.3896 Tier II
12 NC_000067.6 1 15633047 - Kcnb2 NNNAGTAAAGAAATGGTCAG NGG 2 0.35 Tier II
13 NC_000074.6 8 14504141 + Dlgap2 NNNAGTAAAGCAATGGCATG NGG 2 0.2899 Tier II
14 NC_000074.6 8 91508399 - Fto NNNAGTGAAGCACTGGCCAG NGG 2 0.1486 Tier II
15 NC_000068.7 2 23156254 + Yme1l1 NNNAGTAAAGCAATGGCTAG NGA 2 0.0446 Tier II
16 NC_000067.6 1 65238800 - Pikfyve NNNAGTAAAGCAATGGCCAA NGT 2 0.0151 Tier II
17 NC_000071.6 5 44469306 + Ldb2 NNNAGTAAAGCAATGTCAAG NGG 2 0.0 Tier II
18 NC_000080.6 14 119808157 - 4930404K13Rik NNNACTAAAGCAGTGGCCAG NGG 2 0.5124 Tier III
19 NC_000083.6 17 9845452 + Gm34799 NNNAGCAAAGCAATGGACAG NGG 2 0.4242 Tier III
20 NC_000084.6 18 83633554 - Gm31621 NNNAGTAAGGCAATGGCCAG NAG 2 0.1728 Tier III
21 NC_000073.6 7 25512767 + 4732471J01Rik NNNAGTAAAGCAATTGCCAG NGA 2 0.0099 Tier III
22 NC_000076.6 10 72310438 - Gm9923 NNNAGTAAAGCACTGGCCAG NGC 2 0.0047 Tier III
23 NC_000081.6 15 35217169 - Gm33497 NNNAGTAAAGGAATGTCCAG NGG 2 0.0 Tier III
24 NC_000081.6 15 35217169 - Gm52178 NNNAGTAAAGGAATGTCCAG NGG 2 0.0 Tier III
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Other clones with same target sequence:

(none)