Construct: sgRNA BRDN0001145817

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
GTAAAACATCTTCACCACCC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
STK38L (23012)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76629

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000012.12 12 27312604 - STK38L NNNAAACATCTTCACCACCC NGG 0 1.0 Tier I
2 NC_000020.11 20 34294126 - AHCY NNNCAACATCTTCTCCACCC NGG 2 0.1882 Tier I
3 NC_000022.11 22 44862441 + ARHGAP8 NNNAAAGATCTTCAGCACCC NGG 2 0.0235 Tier I
4 NC_000022.11 22 44862441 + PRR5-ARHGAP8 NNNAAAGATCTTCAGCACCC NGG 2 0.0235 Tier I
5 NC_000019.10 19 48985918 - GYS1 NNNAACCATCTTCACCACCC NTG 2 0.0177 Tier I
6 NC_000007.14 7 98879225 - TRRAP NNNAAGCAACTTCACCACCC NGG 2 0.6633 Tier II
7 NC_000009.12 9 18368735 - ADAMTSL1 NNNAAACATATTCACCATCC NGG 2 0.5571 Tier II
8 NC_000001.11 1 49211895 + AGBL4 NNNAATCATCATCACCACCC NGG 2 0.5357 Tier II
9 NC_000004.12 4 72309955 - ADAMTS3 NNNGAAAATCTTCACCACCC NGG 2 0.4688 Tier II
10 NC_000004.12 4 67854382 - TMPRSS11D NNNAAACATCTACACCACTC NGG 2 0.3692 Tier II
11 NC_000011.10 11 17765527 + KCNC1 NNNCAACAGCTTCACCACCC NGG 2 0.2185 Tier II
12 NC_000012.12 12 99231821 - ANKS1B NNNAAACATCTTCACAGCCC NGG 2 0.1765 Tier II
13 NC_000005.10 5 180135596 - RASGEF1C NNNAAAAATCTTCACCTCCC NGG 2 0.1 Tier II
14 NC_000003.12 3 138771722 + PIK3CB NNNAAACATCTGCACCAGCC NGG 2 0.0667 Tier II
15 NC_000016.10 16 27830358 + GSG1L NNNAAACATCATCACCACCC NTG 2 0.0292 Tier II
16 NC_000008.11 8 84459600 - RALYL NNNAAACATCTTTACCACCC NTG 2 0.0273 Tier II
17 NC_000002.12 2 38966293 - ARHGEF33 NNNAAACATCTTCACCCCGC NGG 2 0.0221 Tier II
18 NC_000006.12 6 24559833 + KIAA0319 NNNAAACATCTTCATCACCC NAG 2 0.0173 Tier II
19 NC_000003.12 3 127025080 + PLXNA1 NNNAGACATCTTCACCACCC NGT 2 0.0116 Tier II
20 NC_000013.11 13 108271985 + TNFSF13B NNNAAACATCTTCATCACCC NTG 2 0.0026 Tier II
21 NC_000002.12 2 26207598 + HADHA NNNAAACATCTTCAGCACCC NTG 2 0.0019 Tier II
22 NC_000009.12 9 18368735 - LOC107986990 NNNAAACATATTCACCATCC NGG 2 0.5571 Tier III
23 NC_000005.10 5 133113919 - LOC105379178 NNNAAACATCTCCTCCACCC NGG 2 0.5053 Tier III
24 NC_000007.14 7 29525554 + PRR15-DT NNNAACCATCTTCACAACCC NGG 2 0.4545 Tier III
25 NC_000012.12 12 75678662 - LOC105369844 NNNGAACATCTTCACCACTC NGG 2 0.2885 Tier III
26 NC_000015.10 15 50417633 + AHCYP7 NNNCAACATCTTCTCCACCC NGG 2 0.1882 Tier III
27 NC_000013.11 13 56689114 + LOC105370214 NNNAAACCTCTTCACCACCT NGG 2 0.1286 Tier III
28 NC_000011.10 11 27520436 + BDNF-AS NNNAAACATCTTCCCCACAC NGG 2 0.0918 Tier III
29 NC_000005.10 5 133914805 + WSPAR NNNAAACATCTTCACCCCAC NGG 2 0.0756 Tier III
30 NC_000005.10 5 140738630 - LOC105378198 NNNAAACATGTTCACCAGCC NGG 2 0.0519 Tier III
31 NC_000022.11 22 44862441 + LOC105373062 NNNAAAGATCTTCAGCACCC NGG 2 0.0235 Tier III
32 NC_000005.10 5 80612879 - LINC01337 NNNAAACATCTTCAGCACCC NAG 2 0.013 Tier III
33 NC_000021.9 21 27205211 + LOC102724355 NNNAAACATCTGCACCACCC NGT 2 0.0081 Tier III
34 NC_000021.9 21 27205211 + LOC105372759 NNNAAACATCTGCACCACCC NGT 2 0.0081 Tier III
35 NC_000002.12 2 200761426 + AOX2P NNNAAACATCTTCACCACCT NGT 2 0.0048 Tier III
36 NC_000002.12 2 200761426 + AOX3P-AOX2P NNNAAACATCTTCACCACCT NGT 2 0.0048 Tier III
37 NC_000011.10 11 128329130 - LOC124902787 NNNAAACATCTTCATCACCC NTG 2 0.0026 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000072.6 6 146767474 - Stk38l NNNGAACATCTTCACCACCC NGG 1 0.625 Tier I
2 NC_000082.6 16 94832998 - Kcnj6 NNNGGACATCTTCACCACCC NGG 2 0.45 Tier I
3 NC_000078.6 12 90204535 + Nrxn3 NNNAACCATCTTCAACACCC NGG 2 0.101 Tier I
4 NC_000067.6 1 89143649 + Sh3bp4 NNNAAACAACTTCACCACCC NGA 2 0.0645 Tier I
5 NC_000073.6 7 88031316 + Grm5 NNNAAACATCTCCACCACCC NGG 1 0.9474 Tier II
6 NC_000067.6 1 98458216 - Slco6d1 NNNGAACATCTTCACCACCC NGG 1 0.625 Tier II
7 NC_000074.6 8 86849050 - N4bp1 NNNAAACCTTTTCACCACCC NGG 2 0.4034 Tier II
8 NC_000076.6 10 107516953 + Ptprq NNNAAACATCAGCACCACCC NGG 2 0.375 Tier II
9 NC_000072.6 6 99374289 - Foxp1 NNNAAACATCTTTACCACCA NGG 2 0.35 Tier II
10 NC_000076.6 10 110022445 + Nav3 NNNAGAGATCTTCACCACCC NGG 2 0.3388 Tier II
11 NC_000076.6 10 89476797 + Nr1h4 NNNAAACTTCTTTACCACCC NGG 2 0.3 Tier II
12 NC_000082.6 16 38926106 - Igsf11 NNNAAACATCTTCTCCACAC NGG 2 0.2286 Tier II
13 NC_000068.7 2 29214254 - Ntng2 NNNAGACATCTTCACCACCT NGG 2 0.216 Tier II
14 NC_000081.6 15 96619462 - Slc38a1 NNNAAACATGTTCACCACAC NGG 2 0.1667 Tier II
15 NC_000079.6 13 46587698 - Cap2 NNNAAACATCTTCACTACCA NGG 2 0.1538 Tier II
16 NC_000072.6 6 108547111 + Itpr1 NNNCAACATCTTCAACACCC NGG 2 0.0784 Tier II
17 NC_000078.6 12 44434801 - Nrcam NNNAAACATCTGCACCAGCC NGG 2 0.0667 Tier II
18 NC_000077.6 11 104131733 - Crhr1 NNNAAACATCTTCACCTCTC NGG 2 0.0615 Tier II
19 NC_000078.6 12 84577285 + Vsx2 NNNAAACATCTTCCCCACCC NAG 2 0.0556 Tier II
20 NC_000084.6 18 23009882 + Nol4 NNNAAACATCATCACCACCC NGT 2 0.0121 Tier II
21 NC_000071.6 5 64161210 + Tbc1d1 NNNAAACATCTTCAGCGCCC NGG 2 0.0088 Tier II
22 NC_000067.6 1 98458216 - Gm3555 NNNGAACATCTTCACCACCC NGG 1 0.625 Tier III
23 NC_000076.6 10 107516953 + Gm36177 NNNAAACATCAGCACCACCC NGG 2 0.375 Tier III
24 NC_000075.6 9 32906666 + Gm27162 NNNAAACATCTTCACCACCC NTG 1 0.039 Tier III
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Other clones with same target sequence:

(none)