Construct: sgRNA BRDN0001145817
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GTAAAACATCTTCACCACCC
Original Target:
- Taxon:
- Homo sapiens (human)
- Gene:
- STK38L (23012)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
Reference Sequence | Chromosome | Cut Position | Match Strand | Match Gene | Match Sequence | PAM Seq. | Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. | CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. | Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. | |
---|---|---|---|---|---|---|---|---|---|---|
1 | NC_000012.12 | 12 | 27312604 | - | STK38L | NNNAAACATCTTCACCACCC | NGG | 0 | 1.0 | Tier I |
2 | NC_000020.11 | 20 | 34294126 | - | AHCY | NNNCAACATCTTCTCCACCC | NGG | 2 | 0.1882 | Tier I |
3 | NC_000022.11 | 22 | 44862441 | + | ARHGAP8 | NNNAAAGATCTTCAGCACCC | NGG | 2 | 0.0235 | Tier I |
4 | NC_000022.11 | 22 | 44862441 | + | PRR5-ARHGAP8 | NNNAAAGATCTTCAGCACCC | NGG | 2 | 0.0235 | Tier I |
5 | NC_000019.10 | 19 | 48985918 | - | GYS1 | NNNAACCATCTTCACCACCC | NTG | 2 | 0.0177 | Tier I |
6 | NC_000007.14 | 7 | 98879225 | - | TRRAP | NNNAAGCAACTTCACCACCC | NGG | 2 | 0.6633 | Tier II |
7 | NC_000009.12 | 9 | 18368735 | - | ADAMTSL1 | NNNAAACATATTCACCATCC | NGG | 2 | 0.5571 | Tier II |
8 | NC_000001.11 | 1 | 49211895 | + | AGBL4 | NNNAATCATCATCACCACCC | NGG | 2 | 0.5357 | Tier II |
9 | NC_000004.12 | 4 | 72309955 | - | ADAMTS3 | NNNGAAAATCTTCACCACCC | NGG | 2 | 0.4688 | Tier II |
10 | NC_000004.12 | 4 | 67854382 | - | TMPRSS11D | NNNAAACATCTACACCACTC | NGG | 2 | 0.3692 | Tier II |
11 | NC_000011.10 | 11 | 17765527 | + | KCNC1 | NNNCAACAGCTTCACCACCC | NGG | 2 | 0.2185 | Tier II |
12 | NC_000012.12 | 12 | 99231821 | - | ANKS1B | NNNAAACATCTTCACAGCCC | NGG | 2 | 0.1765 | Tier II |
13 | NC_000005.10 | 5 | 180135596 | - | RASGEF1C | NNNAAAAATCTTCACCTCCC | NGG | 2 | 0.1 | Tier II |
14 | NC_000003.12 | 3 | 138771722 | + | PIK3CB | NNNAAACATCTGCACCAGCC | NGG | 2 | 0.0667 | Tier II |
15 | NC_000016.10 | 16 | 27830358 | + | GSG1L | NNNAAACATCATCACCACCC | NTG | 2 | 0.0292 | Tier II |
16 | NC_000008.11 | 8 | 84459600 | - | RALYL | NNNAAACATCTTTACCACCC | NTG | 2 | 0.0273 | Tier II |
17 | NC_000002.12 | 2 | 38966293 | - | ARHGEF33 | NNNAAACATCTTCACCCCGC | NGG | 2 | 0.0221 | Tier II |
18 | NC_000006.12 | 6 | 24559833 | + | KIAA0319 | NNNAAACATCTTCATCACCC | NAG | 2 | 0.0173 | Tier II |
19 | NC_000003.12 | 3 | 127025080 | + | PLXNA1 | NNNAGACATCTTCACCACCC | NGT | 2 | 0.0116 | Tier II |
20 | NC_000013.11 | 13 | 108271985 | + | TNFSF13B | NNNAAACATCTTCATCACCC | NTG | 2 | 0.0026 | Tier II |
21 | NC_000002.12 | 2 | 26207598 | + | HADHA | NNNAAACATCTTCAGCACCC | NTG | 2 | 0.0019 | Tier II |
22 | NC_000009.12 | 9 | 18368735 | - | LOC107986990 | NNNAAACATATTCACCATCC | NGG | 2 | 0.5571 | Tier III |
23 | NC_000005.10 | 5 | 133113919 | - | LOC105379178 | NNNAAACATCTCCTCCACCC | NGG | 2 | 0.5053 | Tier III |
24 | NC_000007.14 | 7 | 29525554 | + | PRR15-DT | NNNAACCATCTTCACAACCC | NGG | 2 | 0.4545 | Tier III |
25 | NC_000012.12 | 12 | 75678662 | - | LOC105369844 | NNNGAACATCTTCACCACTC | NGG | 2 | 0.2885 | Tier III |
26 | NC_000015.10 | 15 | 50417633 | + | AHCYP7 | NNNCAACATCTTCTCCACCC | NGG | 2 | 0.1882 | Tier III |
27 | NC_000013.11 | 13 | 56689114 | + | LOC105370214 | NNNAAACCTCTTCACCACCT | NGG | 2 | 0.1286 | Tier III |
28 | NC_000011.10 | 11 | 27520436 | + | BDNF-AS | NNNAAACATCTTCCCCACAC | NGG | 2 | 0.0918 | Tier III |
29 | NC_000005.10 | 5 | 133914805 | + | WSPAR | NNNAAACATCTTCACCCCAC | NGG | 2 | 0.0756 | Tier III |
30 | NC_000005.10 | 5 | 140738630 | - | LOC105378198 | NNNAAACATGTTCACCAGCC | NGG | 2 | 0.0519 | Tier III |
31 | NC_000022.11 | 22 | 44862441 | + | LOC105373062 | NNNAAAGATCTTCAGCACCC | NGG | 2 | 0.0235 | Tier III |
32 | NC_000005.10 | 5 | 80612879 | - | LINC01337 | NNNAAACATCTTCAGCACCC | NAG | 2 | 0.013 | Tier III |
33 | NC_000021.9 | 21 | 27205211 | + | LOC102724355 | NNNAAACATCTGCACCACCC | NGT | 2 | 0.0081 | Tier III |
34 | NC_000021.9 | 21 | 27205211 | + | LOC105372759 | NNNAAACATCTGCACCACCC | NGT | 2 | 0.0081 | Tier III |
35 | NC_000002.12 | 2 | 200761426 | + | AOX2P | NNNAAACATCTTCACCACCT | NGT | 2 | 0.0048 | Tier III |
36 | NC_000002.12 | 2 | 200761426 | + | AOX3P-AOX2P | NNNAAACATCTTCACCACCT | NGT | 2 | 0.0048 | Tier III |
37 | NC_000011.10 | 11 | 128329130 | - | LOC124902787 | NNNAAACATCTTCATCACCC | NTG | 2 | 0.0026 | Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
Reference Sequence | Chromosome | Cut Position | Match Strand | Match Gene | Match Sequence | PAM Seq. | Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. | CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. | Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. | |
---|---|---|---|---|---|---|---|---|---|---|
1 | NC_000072.6 | 6 | 146767474 | - | Stk38l | NNNGAACATCTTCACCACCC | NGG | 1 | 0.625 | Tier I |
2 | NC_000082.6 | 16 | 94832998 | - | Kcnj6 | NNNGGACATCTTCACCACCC | NGG | 2 | 0.45 | Tier I |
3 | NC_000078.6 | 12 | 90204535 | + | Nrxn3 | NNNAACCATCTTCAACACCC | NGG | 2 | 0.101 | Tier I |
4 | NC_000067.6 | 1 | 89143649 | + | Sh3bp4 | NNNAAACAACTTCACCACCC | NGA | 2 | 0.0645 | Tier I |
5 | NC_000073.6 | 7 | 88031316 | + | Grm5 | NNNAAACATCTCCACCACCC | NGG | 1 | 0.9474 | Tier II |
6 | NC_000067.6 | 1 | 98458216 | - | Slco6d1 | NNNGAACATCTTCACCACCC | NGG | 1 | 0.625 | Tier II |
7 | NC_000074.6 | 8 | 86849050 | - | N4bp1 | NNNAAACCTTTTCACCACCC | NGG | 2 | 0.4034 | Tier II |
8 | NC_000076.6 | 10 | 107516953 | + | Ptprq | NNNAAACATCAGCACCACCC | NGG | 2 | 0.375 | Tier II |
9 | NC_000072.6 | 6 | 99374289 | - | Foxp1 | NNNAAACATCTTTACCACCA | NGG | 2 | 0.35 | Tier II |
10 | NC_000076.6 | 10 | 110022445 | + | Nav3 | NNNAGAGATCTTCACCACCC | NGG | 2 | 0.3388 | Tier II |
11 | NC_000076.6 | 10 | 89476797 | + | Nr1h4 | NNNAAACTTCTTTACCACCC | NGG | 2 | 0.3 | Tier II |
12 | NC_000082.6 | 16 | 38926106 | - | Igsf11 | NNNAAACATCTTCTCCACAC | NGG | 2 | 0.2286 | Tier II |
13 | NC_000068.7 | 2 | 29214254 | - | Ntng2 | NNNAGACATCTTCACCACCT | NGG | 2 | 0.216 | Tier II |
14 | NC_000081.6 | 15 | 96619462 | - | Slc38a1 | NNNAAACATGTTCACCACAC | NGG | 2 | 0.1667 | Tier II |
15 | NC_000079.6 | 13 | 46587698 | - | Cap2 | NNNAAACATCTTCACTACCA | NGG | 2 | 0.1538 | Tier II |
16 | NC_000072.6 | 6 | 108547111 | + | Itpr1 | NNNCAACATCTTCAACACCC | NGG | 2 | 0.0784 | Tier II |
17 | NC_000078.6 | 12 | 44434801 | - | Nrcam | NNNAAACATCTGCACCAGCC | NGG | 2 | 0.0667 | Tier II |
18 | NC_000077.6 | 11 | 104131733 | - | Crhr1 | NNNAAACATCTTCACCTCTC | NGG | 2 | 0.0615 | Tier II |
19 | NC_000078.6 | 12 | 84577285 | + | Vsx2 | NNNAAACATCTTCCCCACCC | NAG | 2 | 0.0556 | Tier II |
20 | NC_000084.6 | 18 | 23009882 | + | Nol4 | NNNAAACATCATCACCACCC | NGT | 2 | 0.0121 | Tier II |
21 | NC_000071.6 | 5 | 64161210 | + | Tbc1d1 | NNNAAACATCTTCAGCGCCC | NGG | 2 | 0.0088 | Tier II |
22 | NC_000067.6 | 1 | 98458216 | - | Gm3555 | NNNGAACATCTTCACCACCC | NGG | 1 | 0.625 | Tier III |
23 | NC_000076.6 | 10 | 107516953 | + | Gm36177 | NNNAAACATCAGCACCACCC | NGG | 2 | 0.375 | Tier III |
24 | NC_000075.6 | 9 | 32906666 | + | Gm27162 | NNNAAACATCTTCACCACCC | NTG | 1 | 0.039 | Tier III |