Construct: sgRNA BRDN0001145821

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
AGGCTGACACATTAAAGGAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CSNK1G3 (1456)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77361

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000005.10 5 123588442 + CSNK1G3 NNNCTGACACATTAAAGGAG NGG 0 1.0 Tier I
2 NC_000015.10 15 64204935 - CSNK1G1 NNNCTGACACATTAAAAGAG NGA 2 0.0648 Tier I
3 NC_000003.12 3 4092467 - SUMF1 NNNCTGACACATTAAAATAG NGG 2 0.6222 Tier II
4 NC_000001.11 1 156976059 + ARHGEF11 NNNCTAACTCATTAAAGGAG NGG 2 0.6 Tier II
5 NC_000001.11 1 240452332 - FMN2 NNNCTGACTAATTAAAGGAG NGG 2 0.52 Tier II
6 NC_000013.11 13 39454836 + LHFPL6 NNNCTGATACATTTAAGGAG NGG 2 0.4667 Tier II
7 NC_000003.12 3 123802418 + MYLK NNNAAGACACATTAAAGGAG NGG 2 0.4211 Tier II
8 NC_000008.11 8 3397890 - CSMD1 NNNCTGACACCTTAAAGGAT NGG 2 0.28 Tier II
9 NC_000018.10 18 36773239 + FHOD3 NNNCTGACACATTAAAGAGG NGG 2 0.2596 Tier II
10 NC_000023.11 X 31403206 - DMD NNNCTGACACATTACAAGAG NGG 2 0.2545 Tier II
11 NC_000020.11 20 6080215 + FERMT1 NNNCTGACACAGTGAAGGAG NGG 2 0.2333 Tier II
12 NC_000009.12 9 114906166 - TNFSF8 NNNCTGACACATTATAGGAA NGG 2 0.1875 Tier II
13 NC_000006.12 6 43361244 - ZNF318 NNNCTGACAGATTGAAGGAG NGG 2 0.1815 Tier II
14 NC_000003.12 3 138593975 + CEP70 NNNCTGAGACATTAAAGGAG NAG 2 0.1667 Tier II
15 NC_000016.10 16 27873981 - GSG1L NNNTTGACACATTAAGGGAG NGG 2 0.1538 Tier II
16 NC_000002.12 2 214326085 - SPAG16 NNNCTGACACATTATAGGAT NGG 2 0.14 Tier II
17 NC_000011.10 11 92311845 - FAT3 NNNCTGACACATTAAACCAG NGG 2 0.112 Tier II
18 NC_000008.11 8 120547656 + SNTB1 NNNCTGACACATTAAATGAC NGG 2 0.1071 Tier II
19 NC_000022.11 22 33637500 - LARGE1 NNNCTGACACAGTATAGGAG NGG 2 0.1 Tier II
20 NC_000004.12 4 72344818 + ADAMTS3 NNNCTGACACTTTAAAGGAG NGT 2 0.005 Tier II
21 NC_000023.11 X 128331583 - LOC107985698 NNNCTCACACATTAAAAGAG NGG 2 0.6364 Tier III
22 NC_000009.12 9 21535950 - MIR31HG NNNCTGACAGATAAAAGGAG NGG 2 0.2692 Tier III
23 NC_000014.9 14 95334652 - LINC02292 NNNGTGACACCTTAAAGGAG NGG 2 0.2 Tier III
24 NC_000014.9 14 95334652 - LOC124903371 NNNGTGACACCTTAAAGGAG NGG 2 0.2 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000084.6 18 53930293 + Csnk1g3 NNNCTGACACATTAAAAGAG NGG 1 0.9333 Tier I
2 NC_000069.6 3 136872942 - Ppp3ca NNNCTGGCACATTAAAGGAA NGG 2 0.6618 Tier II
3 NC_000074.6 8 77176975 - Nr3c2 NNNCTGACGCATTAAAAGAG NGG 2 0.6222 Tier II
4 NC_000078.6 12 71313537 - Dact1 NNNCTGACACATCAAAGAAG NGG 2 0.5466 Tier II
5 NC_000071.6 5 150581957 + N4bp2l1 NNNTTGAAACATTAAAGGAG NGG 2 0.52 Tier II
6 NC_000071.6 5 131614073 + Auts2 NNNCTGAAACATCAAAGGAG NGG 2 0.5132 Tier II
7 NC_000073.6 7 3430310 - Cacng6 NNNCTGACACATAAGAGGAG NGG 2 0.45 Tier II
8 NC_000080.6 14 59523476 - Cab39l NNNCTGACAGATTAAAGGAA NGG 2 0.3646 Tier II
9 NC_000069.6 3 58687176 + Siah2 NNNCTGACACTTTAAAGGAG NGG 1 0.3077 Tier II
10 NC_000067.6 1 73933305 - Tns1 NNNCTGACTCAGTAAAGGAG NGG 2 0.3 Tier II
11 NC_000067.6 1 58062955 - Aox1 NNNCTGACTCATTAAAGCAG NGG 2 0.2857 Tier II
12 NC_000071.6 5 98388175 - Cfap299 NNNCTGACACATCACAGGAG NGG 2 0.2153 Tier II
13 NC_000085.6 19 15979180 + Cep78 NNNCTGTCACATTAAAGGAC NGG 2 0.1875 Tier II
14 NC_000083.6 17 13133109 - Unc93a NNNCTGGCACATGAAAGGAG NGG 2 0.1841 Tier II
15 NC_000070.6 4 84453693 + Bnc2 NNNCTGTCACTTTAAAGGAG NGG 2 0.1346 Tier II
16 NC_000079.6 13 81254873 + Adgrv1 NNNCTGACACATAAAGGGAG NGG 2 0.1331 Tier II
17 NC_000074.6 8 70112357 + Ncan NNNCTGACACTTTCAAGGAG NGG 2 0.0659 Tier II
18 NC_000082.6 16 41773394 + Lsamp NNNCTGACACATTAAAGAAG NTG 2 0.027 Tier II
19 NC_000068.7 2 40940893 + Lrp1b NNNCTGACACATTAAAGGAG NAA 2 0.0 Tier II
20 NC_000083.6 17 49812022 + Kif6 NNNCTGACACATTAAAGGAG NTT 2 0.0 Tier II
21 NC_000086.7 X 53418781 + Gm14584 NNNCTGACACATTAAAAGAG NGG 1 0.9333 Tier III
22 NC_000076.6 10 99634827 - Gm20110 NNNCTGACACATCAAAAGAG NGG 2 0.7368 Tier III
23 NC_000068.7 2 172976302 + Gm52538 NNNCTGACTCACTAAAGGAG NGG 2 0.5684 Tier III
24 NC_000070.6 4 24887946 - Gm46848 NNNGTGACACATCAAAGGAG NGG 2 0.3947 Tier III
25 NC_000070.6 4 24893096 - Gm46848 NNNGTGACACATCAAAGGAG NGG 2 0.3947 Tier III
26 NC_000082.6 16 93628930 + Gm5678 NNNCTTCCACATTAAAGGAG NGG 2 0.2917 Tier III
27 NC_000083.6 17 13133109 - Gm36277 NNNCTGGCACATGAAAGGAG NGG 2 0.1841 Tier III
28 NC_000079.6 13 9032366 - Gm36264 NNNCTGACACATGCAAGGAG NGG 2 0.0559 Tier III
29 NC_000079.6 13 44024857 + Gm33489 NNNCTGACACATTAAAGGAG NTA 2 0.0 Tier III
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Other clones with same target sequence:

(none)