Construct: sgRNA BRDN0001145862
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TGCCGTTAAACAAATCCTGG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- CAMK4 (814)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77226
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000005.10 |
5 |
111394769 |
+ |
CAMK4 |
NNNCGTTAAACAAATCCTGG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000005.10 |
5 |
31266243 |
- |
CDH6 |
NNNCATTAAAAAAATCCTGG |
NGG |
2 |
0.65 |
Tier II |
| 3 |
NC_000015.10 |
15 |
27157279 |
+ |
GABRG3 |
NNNCGTTAAACAAATCCTTA |
NGG |
2 |
0.625 |
Tier II |
| 4 |
NC_000001.11 |
1 |
230096526 |
- |
GALNT2 |
NNNCCTTAAAGAAATCCTGG |
NGG |
2 |
0.1964 |
Tier II |
| 5 |
NC_000006.12 |
6 |
38557967 |
- |
BTBD9 |
NNNCGTTAAACAACTCTTGG |
NGG |
2 |
0.1 |
Tier II |
| 6 |
NC_000009.12 |
9 |
90529776 |
- |
LINC01501 |
NNNCGTGAAGCAAATCCTGG |
NGG |
2 |
0.3268 |
Tier III |
| 7 |
NC_000012.12 |
12 |
131312195 |
- |
LOC124903055 |
NNNCATTAAACAAATCCTGG |
NAG |
2 |
0.2247 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000085.6 |
19 |
22504747 |
- |
Trpm3 |
NNNCCTTAAACAAATACTGG |
NGG |
2 |
0.7857 |
Tier II |
| 2 |
NC_000079.6 |
13 |
15620898 |
- |
Gli3 |
NNNTGATAAACAAATCCTGG |
NGG |
2 |
0.6933 |
Tier II |
| 3 |
NC_000081.6 |
15 |
92756398 |
- |
Pdzrn4 |
NNNAGTTAAACAAATCCTGT |
NGG |
2 |
0.5895 |
Tier II |
| 4 |
NC_000074.6 |
8 |
25863161 |
+ |
Kcnu1 |
NNNTGTTAAACAGATCCTGG |
NGG |
2 |
0.5217 |
Tier II |
| 5 |
NC_000076.6 |
10 |
60302828 |
+ |
Cdh23 |
NNNAGTTAAACAAAACCTGG |
NGG |
2 |
0.4875 |
Tier II |
| 6 |
NC_000076.6 |
10 |
45708068 |
- |
Hace1 |
NNNGATTAAACAAATCCTGG |
NGG |
2 |
0.4333 |
Tier II |
| 7 |
NC_000076.6 |
10 |
127647345 |
+ |
Stat6 |
NNNCATTAAAGAAATCCTGG |
NGG |
2 |
0.2167 |
Tier II |
| 8 |
NC_000084.6 |
18 |
61828158 |
- |
Ablim3 |
NNNCGGTAAACAACTCCTGG |
NGG |
2 |
0.1224 |
Tier II |
| 9 |
NC_000085.6 |
19 |
22504747 |
- |
Gm33649 |
NNNCCTTAAACAAATACTGG |
NGG |
2 |
0.7857 |
Tier III |
| 10 |
NC_000074.6 |
8 |
52772769 |
- |
Gm9685 |
NNNAGTTAAAAAAATCCTGG |
NGG |
2 |
0.6316 |
Tier III |
| 11 |
NC_000068.7 |
2 |
162678762 |
- |
Gm14246 |
NNNCGTTAAGCAAATCCCGG |
NGG |
2 |
0.2381 |
Tier III |
Other clones with same target sequence:
(none)
