Construct: sgRNA BRDN0001145866
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GGAAGGCAAGAGACCGTTTG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- TXK (7294)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76359
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000004.12 |
4 |
48112312 |
- |
TXK |
NNNAGGCAAGAGACCGTTTG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000010.11 |
10 |
80413931 |
+ |
PRXL2A |
NNNGGGTAAGAGACCGTTTG |
NGG |
2 |
0.5078 |
Tier II |
| 3 |
NC_000003.12 |
3 |
15271294 |
+ |
SH3BP5 |
NNNAGGCAGGAGACCGCTTG |
NGG |
2 |
0.4706 |
Tier II |
| 4 |
NC_000007.14 |
7 |
146194413 |
+ |
CNTNAP2 |
NNNAGGCAAGAGAACGTTTG |
NAG |
2 |
0.0907 |
Tier II |
| 5 |
NC_000010.11 |
10 |
50211766 |
- |
ASAH2 |
NNNAGGCAAGAGAACGTTTG |
NTG |
2 |
0.0136 |
Tier II |
| 6 |
NC_000002.12 |
2 |
211710773 |
- |
ERBB4 |
NNNAGGCAAGAGACAGTTTG |
NGT |
2 |
0.0036 |
Tier II |
| 7 |
NC_000017.11 |
17 |
47188812 |
+ |
CDC27 |
NNNAGGCAAGAGAGCGCTTG |
NGG |
2 |
0.0 |
Tier II |
| 8 |
NC_000007.14 |
7 |
70765141 |
- |
AUTS2 |
NNNAGGCCAGAGAGCGTTTG |
NGG |
2 |
0.0 |
Tier II |
| 9 |
NC_000009.12 |
9 |
87623404 |
+ |
DAPK1 |
NNNAGGCTAGAGACCTTTTG |
NGG |
2 |
0.0 |
Tier II |
| 10 |
NC_000012.12 |
12 |
53185795 |
- |
ZNF740 |
NNNAGGTAAGAGACCCTTTG |
NGG |
2 |
0.0 |
Tier II |
| 11 |
NC_000022.11 |
22 |
23392107 |
+ |
ZDHHC8BP |
NNNAGGCAAGAGATCGCTTG |
NGG |
2 |
0.5176 |
Tier III |
| 12 |
NC_000014.9 |
14 |
86341756 |
- |
LINC02309 |
NNNAGGCAAGAGACCGTGTG |
NAG |
2 |
0.0864 |
Tier III |
| 13 |
NC_000015.10 |
15 |
80335140 |
- |
LINC00927 |
NNNAGGCAACAGACTGTTTG |
NGG |
2 |
0.0267 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000067.6 |
1 |
10706862 |
+ |
Cpa6 |
NNNAGGCAAGAGACTGTGTG |
NGG |
2 |
0.0222 |
Tier II |
| 2 |
NC_000068.7 |
2 |
6863051 |
+ |
Celf2 |
NNNAGGCAAGAGACTGTTTG |
NGA |
2 |
0.0046 |
Tier II |
| 3 |
NC_000074.6 |
8 |
71547911 |
- |
Mvb12a |
NNNAGGCAAGAGACCCTTTG |
NGC |
2 |
0.0 |
Tier II |
Other clones with same target sequence:
(none)
