Construct: sgRNA BRDN0001145874
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AACTAGCCCGAGCAGCTTCG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- NO_SITE control
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 80182
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000012.12 |
12 |
46257956 |
+ |
SLC38A1 |
NNNTAGACAGAGCAGCTTCG |
NGG |
2 |
0.6429 |
Tier II |
| 2 |
NC_000017.11 |
17 |
83008732 |
+ |
B3GNTL1 |
NNNAAGCCTGAGCAGCTTCG |
NGG |
2 |
0.4167 |
Tier II |
| 3 |
NC_000014.9 |
14 |
105248468 |
+ |
BRF1 |
NNNAAGCTCGAGCAGCTTCG |
NGG |
2 |
0.4167 |
Tier II |
| 4 |
NC_000009.12 |
9 |
2146662 |
+ |
SMARCA2 |
NNNTAGGCAGAGCAGCTTCG |
NGG |
2 |
0.4034 |
Tier II |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000069.6 |
3 |
157618240 |
+ |
Ptger3 |
NNNCAGCCAGAGCAGCTTCG |
NGG |
2 |
0.5546 |
Tier II |
| 2 |
NC_000074.6 |
8 |
11029135 |
+ |
9530052E02Rik |
NNNCAGCCCGAGCACCTTCG |
NGG |
2 |
0.1765 |
Tier III |
Other clones with same target sequence:
(none)
