Construct: sgRNA BRDN0001145885
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GGGACGCGAAAGAAACCAGT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- NO_SITE control
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 80196
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000010.11 |
10 |
114213585 |
+ |
TDRD1 |
NNNACGTGAAAGAAACCAGT |
NGT |
2 |
0.0131 |
Tier I |
| 2 |
NC_000012.12 |
12 |
121804648 |
- |
SETD1B |
NNNACGCGAAAGAATCCAAT |
NGG |
2 |
0.1429 |
Tier II |
| 3 |
NC_000015.10 |
15 |
42472070 |
+ |
ZNF106 |
NNNACGAGAAAGAAACCAGT |
NGA |
2 |
0.0521 |
Tier II |
| 4 |
NC_000016.10 |
16 |
86193403 |
+ |
LOC124903743 |
NNNACGCGATAGAAACCAGA |
NGG |
2 |
0.4963 |
Tier III |
| 5 |
NC_000021.9 |
21 |
14072922 |
- |
LOC105377134 |
NNNACGGGAAAGAAAGCAGT |
NGG |
2 |
0.0724 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000072.6 |
6 |
42365564 |
+ |
Epha1 |
NNNACTCGATAGAAACCAGT |
NGG |
2 |
0.5882 |
Tier I |
| 2 |
NC_000071.6 |
5 |
39622845 |
+ |
Hs3st1 |
NNNATGTGAAAGAAACCAGT |
NGG |
2 |
0.517 |
Tier II |
| 3 |
NC_000083.6 |
17 |
46451174 |
+ |
Ttbk1 |
NNNACTCGAAAGAAACCAAT |
NGG |
2 |
0.4762 |
Tier II |
| 4 |
NC_000069.6 |
3 |
148832814 |
- |
Adgrl2 |
NNNAAGCCAAAGAAACCAGT |
NGG |
2 |
0.3516 |
Tier II |
| 5 |
NC_000077.6 |
11 |
57094996 |
- |
Gria1 |
NNNAGGGGAAAGAAACCAGT |
NGG |
2 |
0.2824 |
Tier II |
| 6 |
NC_000073.6 |
7 |
80215981 |
- |
Sema4b |
NNNAAGGGAAAGAAACCAGT |
NGG |
2 |
0.2689 |
Tier II |
Other clones with same target sequence:
(none)
