Construct: sgRNA BRDN0001145890
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- ATGACGAGCCAACTTATGCT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PEAK1 (79834)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75853
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000015.10 |
15 |
77180079 |
- |
PEAK1 |
NNNACGAGCCAACTTATGCT |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000015.10 |
15 |
40790631 |
+ |
DNAJC17 |
NNNACCAGCCACCTTATGCT |
NGG |
2 |
0.1794 |
Tier II |
| 3 |
NC_000023.11 |
X |
54354577 |
+ |
WNK3 |
NNNAAGAGCCAACTCATGCT |
NGG |
2 |
0.1558 |
Tier II |
| 4 |
NC_000020.11 |
20 |
22512295 |
- |
LOC105372564 |
NNNACGAGCCACCTTTTGCT |
NGG |
2 |
0.0 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000080.6 |
14 |
34998724 |
+ |
Grid1 |
NNNACTAGCCAACTTATTCT |
NGG |
2 |
0.4444 |
Tier II |
| 2 |
NC_000069.6 |
3 |
145083361 |
- |
Clca2 |
NNNACCAGCCAAGTTATGCT |
NGG |
2 |
0.093 |
Tier II |
| 3 |
NC_000080.6 |
14 |
35430144 |
+ |
Grid1 |
NNNAAGAGCCAAGTTATGCT |
NGG |
2 |
0.0779 |
Tier II |
| 4 |
NC_000067.6 |
1 |
121450358 |
+ |
Ccdc93 |
NNNACGAGCCCAGTTATGCT |
NGG |
2 |
0.0545 |
Tier II |
| 5 |
NC_000075.6 |
9 |
41002241 |
- |
Crtam |
NNNACGAGCCACCTGATGCT |
NGG |
2 |
0.0132 |
Tier II |
Other clones with same target sequence:
(none)
