Construct: sgRNA BRDN0001145896
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CGCCAAATAGATGTCCCCGA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- CSNK1A1 (1452)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76190
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000005.10 |
5 |
149550880 |
+ |
CSNK1A1 |
NNNCAAATAGATGTCCCCGA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000002.12 |
2 |
196892403 |
+ |
PGAP1 |
NNNCACATAGATGTCCCTGA |
NGG |
2 |
0.2922 |
Tier I |
| 3 |
NC_000018.10 |
18 |
55465501 |
+ |
TCF4 |
NNNCAAACAGATGTCCCCTA |
NGG |
2 |
0.6667 |
Tier II |
| 4 |
NC_000002.12 |
2 |
1422431 |
- |
TPO |
NNNCAAATAGAAGTCCCTGA |
NGG |
2 |
0.5143 |
Tier II |
| 5 |
NC_000009.12 |
9 |
114477490 |
- |
WHRN |
NNNCCAATAGATGTCCCCTA |
NGG |
2 |
0.3333 |
Tier II |
| 6 |
NC_000001.11 |
1 |
14799938 |
+ |
KAZN |
NNNATAATAGATGTCCCCGA |
NGG |
2 |
0.3062 |
Tier II |
| 7 |
NC_000016.10 |
16 |
83010784 |
- |
CDH13 |
NNNCAAGTAGATGTCCCCGC |
NGG |
2 |
0.1604 |
Tier II |
| 8 |
NC_000005.10 |
5 |
123183250 |
+ |
PRDM6 |
NNNCAAATAGATGCCCCAGA |
NGG |
2 |
0.1538 |
Tier II |
| 9 |
NC_000023.11 |
X |
65312776 |
- |
ZC3H12B |
NNNCAAATAGATGTCCCAGA |
NAG |
2 |
0.1396 |
Tier II |
| 10 |
NC_000002.12 |
2 |
58108423 |
- |
VRK2 |
NNNCAAATAGATGTCACGGA |
NGG |
2 |
0.1333 |
Tier II |
| 11 |
NC_000012.12 |
12 |
125462613 |
+ |
TMEM132B |
NNNTAAATAGATGTCCCCGA |
NGT |
2 |
0.0129 |
Tier II |
| 12 |
NC_000018.10 |
18 |
55465501 |
+ |
TCF4-AS1 |
NNNCAAACAGATGTCCCCTA |
NGG |
2 |
0.6667 |
Tier III |
| 13 |
NC_000005.10 |
5 |
88674078 |
- |
MIR9-2HG |
NNNCAAATAGATGACCCCTA |
NGG |
2 |
0.4127 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000067.6 |
1 |
171697749 |
+ |
Cd48 |
NNNCAAATGGATGTCCCCAA |
NGG |
2 |
0.4762 |
Tier II |
| 2 |
NC_000073.6 |
7 |
105804917 |
+ |
Mrpl17 |
NNNCAAATAGAAGTCCCAGA |
NGG |
2 |
0.4308 |
Tier II |
| 3 |
NC_000077.6 |
11 |
57290776 |
- |
Gria1 |
NNNGAAATAGATGTCCTCGA |
NGG |
2 |
0.2333 |
Tier II |
Other clones with same target sequence:
(none)
