Construct: sgRNA BRDN0001145902
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- ATAACAGAACGATATAAAGT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- DCLK1 (9201)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76746
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000013.11 |
13 |
35836090 |
- |
DCLK1 |
NNNACAGAACGATATAAAGT |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000007.14 |
7 |
89010370 |
+ |
ZNF804B |
NNNACAAAATGATATAAAGT |
NGG |
2 |
0.9412 |
Tier II |
| 3 |
NC_000011.10 |
11 |
40638128 |
- |
LRRC4C |
NNNACAGAAAAATATAAAGT |
NGG |
2 |
0.8667 |
Tier II |
| 4 |
NC_000003.12 |
3 |
56054152 |
+ |
ERC2 |
NNNAGAGAATGATATAAAGT |
NGG |
2 |
0.5647 |
Tier II |
| 5 |
NC_000020.11 |
20 |
9445742 |
+ |
PLCB4 |
NNNACAGAACAATTTAAAGT |
NGG |
2 |
0.5333 |
Tier II |
| 6 |
NC_000006.12 |
6 |
121107519 |
- |
TBC1D32 |
NNNACATATCGATATAAAGT |
NGG |
2 |
0.3429 |
Tier II |
| 7 |
NC_000006.12 |
6 |
37008423 |
- |
FGD2 |
NNNACAGAACAATATAAAGT |
NAG |
2 |
0.2593 |
Tier II |
| 8 |
NC_000015.10 |
15 |
85613947 |
+ |
AKAP13 |
NNNACAGAAAGCTATAAAGT |
NGG |
2 |
0.2281 |
Tier II |
| 9 |
NC_000014.9 |
14 |
73862550 |
+ |
PTGR2 |
NNNACAGAAGTATATAAAGT |
NGG |
2 |
0.1496 |
Tier II |
| 10 |
NC_000002.12 |
2 |
24812224 |
- |
CENPO |
NNNACAGAATGATATAAAGT |
NTG |
2 |
0.0367 |
Tier II |
| 11 |
NC_000003.12 |
3 |
134635829 |
+ |
CEP63 |
NNNACAGAACGATATAAAGC |
NGA |
2 |
0.0063 |
Tier II |
| 12 |
NC_000003.12 |
3 |
134635829 |
+ |
KY |
NNNACAGAACGATATAAAGC |
NGA |
2 |
0.0063 |
Tier II |
| 13 |
NC_000013.11 |
13 |
77567836 |
- |
SCEL |
NNNACAGAAAGATATCAAGT |
NGG |
2 |
0.0 |
Tier II |
| 14 |
NC_000008.11 |
8 |
6637613 |
+ |
MCPH1 |
NNNACAGAACGATTTTAAGT |
NGG |
2 |
0.0 |
Tier II |
| 15 |
NC_000009.12 |
9 |
41041323 |
+ |
PGM5P2 |
NNNACAGAATAATATAAAGT |
NGG |
2 |
0.9412 |
Tier III |
| 16 |
NC_000016.10 |
16 |
29114284 |
+ |
RRN3P2 |
NNNACAGAAATATATAAAGT |
NGG |
2 |
0.3333 |
Tier III |
| 17 |
NC_000008.11 |
8 |
93636359 |
- |
CIBAR1-DT |
NNNACAGAAAGATATAAAGT |
NGA |
2 |
0.0602 |
Tier III |
| 18 |
NC_000008.11 |
8 |
6637613 |
+ |
MCPH1-AS1 |
NNNACAGAACGATTTTAAGT |
NGG |
2 |
0.0 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000069.6 |
3 |
154596014 |
+ |
Tyw3 |
NNNACAGAACAATATAAATT |
NGG |
2 |
0.6667 |
Tier II |
| 2 |
NC_000070.6 |
4 |
131939395 |
- |
Epb41 |
NNNGCAGAATGATATAAAGT |
NGG |
2 |
0.5882 |
Tier II |
| 3 |
NC_000069.6 |
3 |
133354910 |
+ |
Ppa2 |
NNNACAGAACAATTTAAAGT |
NGG |
2 |
0.5333 |
Tier II |
| 4 |
NC_000067.6 |
1 |
14912677 |
+ |
Trpa1 |
NNNACATAACGACATAAAGT |
NGG |
2 |
0.4511 |
Tier II |
| 5 |
NC_000081.6 |
15 |
96617240 |
+ |
Slc38a1 |
NNNACTGAACGATATAAACT |
NGG |
2 |
0.3202 |
Tier II |
| 6 |
NC_000075.6 |
9 |
97798192 |
- |
Clstn2 |
NNNACAGAATGTTATAAAGT |
NGG |
2 |
0.3137 |
Tier II |
| 7 |
NC_000082.6 |
16 |
21927745 |
- |
Map3k13 |
NNNACAGAGCTATATAAAGT |
NGG |
2 |
0.2564 |
Tier II |
| 8 |
NC_000079.6 |
13 |
8718385 |
- |
Adarb2 |
NNNACAGAACTATTTAAAGT |
NGG |
2 |
0.2051 |
Tier II |
| 9 |
NC_000067.6 |
1 |
174790560 |
+ |
Fmn2 |
NNNACAGAACGATATAAACT |
NAG |
2 |
0.1162 |
Tier II |
| 10 |
NC_000069.6 |
3 |
130433126 |
- |
Col25a1 |
NNNACAGAACGATGTAGAGT |
NGG |
2 |
0.0824 |
Tier II |
| 11 |
NC_000076.6 |
10 |
64578958 |
+ |
Ctnna3 |
NNNACAGAACAATATAAAGT |
NGA |
2 |
0.0694 |
Tier II |
| 12 |
NC_000069.6 |
3 |
76575028 |
- |
Fstl5 |
NNNAGAGAACGATATAAAGT |
NGT |
2 |
0.0097 |
Tier II |
| 13 |
NC_000073.6 |
7 |
10198609 |
+ |
Gm20679 |
NNNACATAAAGATATAAAGT |
NGG |
2 |
0.4952 |
Tier III |
| 14 |
NC_000080.6 |
14 |
24729283 |
- |
Gm31775 |
NNNACAGAAAGATATGAAGT |
NGG |
2 |
0.1667 |
Tier III |
Other clones with same target sequence:
(none)
