Construct: sgRNA BRDN0001145907
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AAGACTCGTAGGAATCGATG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PHKG2 (5261)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77384
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000016.10 |
16 |
30751559 |
- |
PHKG2 |
NNNACTCGTAGGAATCGATG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000005.10 |
5 |
151486714 |
+ |
SLC36A1 |
NNNTCTCGTAGGAATCAATG |
NGG |
2 |
0.5939 |
Tier II |
| 3 |
NC_000019.10 |
19 |
11197253 |
+ |
KANK2 |
NNNACTGGGAGGAATCGATG |
NGG |
2 |
0.2913 |
Tier II |
| 4 |
NC_000002.12 |
2 |
130990605 |
+ |
ARHGEF4 |
NNNACTCGTAGGAATCAAAG |
NGG |
2 |
0.2667 |
Tier II |
| 5 |
NC_000022.11 |
22 |
35968890 |
- |
RBFOX2 |
NNNAATCGTAGGAATTGATG |
NGG |
2 |
0.1758 |
Tier II |
| 6 |
NC_000011.10 |
11 |
28577806 |
+ |
LINC02758 |
NNNACTCGTAGCAATAGATG |
NGG |
2 |
0.5294 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000073.6 |
7 |
127577985 |
- |
Phkg2 |
NNNACTCGTAGGAATCGATG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000080.6 |
14 |
13939829 |
- |
Gm11100 |
NNNACTCCAAGGAATCGATG |
NGG |
2 |
0.5714 |
Tier II |
| 3 |
NC_000069.6 |
3 |
31290781 |
+ |
Slc7a14 |
NNNACTCTTAGGAATCTATG |
NGG |
2 |
0.1563 |
Tier II |
| 4 |
NC_000076.6 |
10 |
63949017 |
- |
Lrrtm3 |
NNNACTCCTAGGAATCTATG |
NGG |
2 |
0.1538 |
Tier II |
| 5 |
NC_000076.6 |
10 |
63949017 |
- |
Ctnna3 |
NNNACTCCTAGGAATCTATG |
NGG |
2 |
0.1538 |
Tier II |
| 6 |
NC_000085.6 |
19 |
16545532 |
- |
Gna14 |
NNNACTCCTAGGAATCTATG |
NGG |
2 |
0.1538 |
Tier II |
| 7 |
NC_000081.6 |
15 |
66110816 |
- |
Kcnq3 |
NNNACTCGTAAGAATGGATG |
NGG |
2 |
0.1538 |
Tier II |
| 8 |
NC_000067.6 |
1 |
163029870 |
+ |
Mroh9 |
NNNACTCCTAGGAATCCATG |
NGG |
2 |
0.1448 |
Tier II |
| 9 |
NC_000086.7 |
X |
69562006 |
- |
Aff2 |
NNNACTGGTAGGAATGGATG |
NGG |
2 |
0.0724 |
Tier II |
| 10 |
NC_000067.6 |
1 |
130966824 |
- |
Gm41952 |
NNNACTCGTTGGAATCGATG |
NCG |
2 |
0.0945 |
Tier III |
Other clones with same target sequence:
(none)
