Construct: sgRNA BRDN0001145927

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
AATGAAAGCATGGACCATGG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RPS6KB1 (6198)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
75613

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000017.11 17 59910588 + RPS6KB1 NNNGAAAGCATGGACCATGG NGG 0 1.0 Tier I
2 NC_000013.11 13 98945361 - DOCK9 NNNGACAACATGGACCATGG NGG 2 0.4545 Tier II
3 NC_000001.11 1 220790375 + MTARC1 NNNGAAAGCAGGAACCATGG NGG 2 0.3692 Tier II
4 NC_000006.12 6 136650090 + MAP3K5 NNNGAAAGCAAGCACCATGG NGG 2 0.3158 Tier II
5 NC_000012.12 12 72374085 + TRHDE NNNGAAGGCATGGACCAGGG NGG 2 0.2353 Tier II
6 NC_000020.11 20 8141907 + PLCB1 NNNGAAATCATGGACCATGG NAG 2 0.162 Tier II
7 NC_000016.10 16 3982160 + ADCY9 NNNGACAGCATGGCCCATGG NGG 2 0.0974 Tier II
8 NC_000007.14 7 75679636 - HIP1 NNNGAAAGCCTGGCCCATGG NGG 2 0.0714 Tier II
9 NC_000008.11 8 74432201 + GDAP1 NNNGAAAGCAGGGACCCTGG NGG 2 0.0706 Tier II
10 NC_000016.10 16 83679818 + CDH13 NNNGAAAGCAGGGACCCTGG NGG 2 0.0706 Tier II
11 NC_000003.12 3 62618624 - CADPS NNNGAAAGCATGGAACATGG NAG 2 0.0576 Tier II
12 NC_000010.11 10 17417362 - ST8SIA6 NNNGAAAGAATGGATCATGG NGG 2 0.0571 Tier II
13 NC_000012.12 12 111767385 - ALDH2 NNNGAAAGCAAGGACCATGG NGA 2 0.0521 Tier II
14 NC_000002.12 2 26893176 - DPYSL5 NNNGAAAGCATGGCCCTTGG NGG 2 0.0286 Tier II
15 NC_000019.10 19 58088579 + ZSCAN18 NNNGATAGCATGGACCATGG NGC 2 0.0159 Tier II
16 NC_000003.12 3 29488122 - RBMS3 NNNGAAAGCATGGACCTTGG NGC 2 0.003 Tier II
17 NC_000002.12 2 14241760 + LINC00276 NNNGAAAGCATGGACAATGT NGG 2 0.7 Tier III
18 NC_000012.12 12 107914376 - LOC105369964 NNNAAAAGCATGCACCATGG NGG 2 0.3789 Tier III
19 NC_000007.14 7 100246791 - CASTOR3P NNNGAGAGCCTGGACCATGG NGG 2 0.2381 Tier III
20 NC_000002.12 2 214868038 + SNHG31 NNNGAAAGCATTCACCATGG NGG 2 0.1619 Tier III
21 NC_000016.10 16 83679818 + LOC124900603 NNNGAAAGCAGGGACCCTGG NGG 2 0.0706 Tier III
22 NC_000010.11 10 101741623 + LOC105378458 NNNGAAAGCATGGACCACGG NGA 2 0.0298 Tier III
23 NC_000004.12 4 184378109 + LINC02362 NNNGAAGGCATGGACCATGG NTG 2 0.0275 Tier III
24 NC_000014.9 14 98222254 + LOC105370656 NNNGAAAGCATGGAGCATGG NAG 2 0.013 Tier III
25 NC_000007.14 7 89491285 + LOC105375387 NNNGAAAGCATGTACCATGG NTG 2 0.0117 Tier III
26 NC_000010.11 10 95889010 - ENTPD1-AS1 NNNGAAAGCATGGCGCATGG NGG 2 0.0107 Tier III
27 NC_000007.14 7 118363781 + LOC124901815 NNNGAAAGCATGGCCCATGG NGC 2 0.0048 Tier III
28 NC_000004.12 4 14195155 - LOC124900670 NNNGAAAGCATGGACCATGG NAT 2 0.0 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000077.6 11 86535435 - Rps6kb1 NNNGAAAGCATGGACCATGG NGG 0 1.0 Tier I
2 NC_000076.6 10 20029192 + Map3k5 NNNGAAAGCAGGGACCATGG NGC 2 0.0089 Tier I
3 NC_000070.6 4 136607114 - Tex46 NNNGGAAGCATGGACCATGG NGG 1 0.72 Tier II
4 NC_000071.6 5 36257938 - Sorcs2 NNNGAAGGCATGAACCATGG NGG 2 0.6516 Tier II
5 NC_000078.6 12 4312160 - Ncoa1 NNNGAAACCATGGACCATGA NGG 2 0.5769 Tier II
6 NC_000073.6 7 51633747 + Slc17a6 NNNGAAAACGTGGACCATGG NGG 2 0.5556 Tier II
7 NC_000072.6 6 42627140 - Tcaf2 NNNAAAACCATGGACCATGG NGG 2 0.5538 Tier II
8 NC_000068.7 2 157235122 + Mroh8 NNNGACAGCATGGACCATGG NGG 1 0.4545 Tier II
9 NC_000079.6 13 93142370 - Cmya5 NNNGACAGCATGGACCATGG NGG 1 0.4545 Tier II
10 NC_000080.6 14 31241101 - Phf7 NNNGATACCATGGACCATGG NGG 2 0.4396 Tier II
11 NC_000072.6 6 64197112 - Grid2 NNNGAAAGGATGGACCATGT NGG 2 0.4333 Tier II
12 NC_000074.6 8 99076769 + Cdh8 NNNGAAAGGATGGACCATGT NGG 2 0.4333 Tier II
13 NC_000076.6 10 52109395 - Ros1 NNNGAAAGGATGGACCATGT NGG 2 0.4333 Tier II
14 NC_000080.6 14 57594963 + Xpo4 NNNGAAAGGATGGACCATGT NGG 2 0.4333 Tier II
15 NC_000083.6 17 14801052 - Wdr27 NNNGAAAGGATGGACCATGT NGG 2 0.4333 Tier II
16 NC_000071.6 5 36257898 - Sorcs2 NNNGAAAGCATGCACCATGA NGG 2 0.3947 Tier II
17 NC_000073.6 7 123085596 + Tnrc6a NNNGAAATGATGGACCATGG NGG 2 0.3869 Tier II
18 NC_000079.6 13 55279734 - Nsd1 NNNGCAAGCATGGACCATAG NGG 2 0.3571 Tier II
19 NC_000069.6 3 96445940 - BC107364 NNNGCGAGCATGGACCATGG NGG 2 0.3571 Tier II
20 NC_000086.7 X 141428350 - Col4a6 NNNGAAAGCATGGTCCAAGG NGG 2 0.3556 Tier II
21 NC_000076.6 10 60382123 + Cdh23 NNNGAATGGATGGACCATGG NGG 2 0.2708 Tier II
22 NC_000076.6 10 60382123 + Vsir NNNGAATGGATGGACCATGG NGG 2 0.2708 Tier II
23 NC_000081.6 15 10271329 - Prlr NNNGAACCCATGGACCATGG NGG 2 0.2692 Tier II
24 NC_000083.6 17 81509229 - Slc8a1 NNNGAAAGCATGGTCCATGC NGG 2 0.2286 Tier II
25 NC_000076.6 10 3260029 - H60c NNNGAAAGCCTGGACCATTG NGG 2 0.2222 Tier II
26 NC_000076.6 10 22286084 + H60b NNNGAAAGCCTGGACCATTG NGG 2 0.2222 Tier II
27 NC_000067.6 1 88364985 - Trpm8 NNNGAAAGCAAGGACCATGG NAG 2 0.1944 Tier II
28 NC_000073.6 7 37420291 + Zfp536 NNNTCAAGCATGGACCATGG NGG 2 0.1818 Tier II
29 NC_000080.6 14 21588916 - Kat6b NNNGAAAGCATGGACCATGT NAG 2 0.1815 Tier II
30 NC_000071.6 5 77305684 + Noa1 NNNGAAAGGATGGACCATGG NAG 2 0.1605 Tier II
31 NC_000068.7 2 102703828 - Slc1a2 NNNGAAAGGATGGAACATGG NGG 2 0.1376 Tier II
32 NC_000085.6 19 22746369 - Trpm3 NNNGAATGCATGGACCATGG NAG 2 0.1134 Tier II
33 NC_000079.6 13 108333285 - Depdc1b NNNGAAAGGATGGACGATGG NGG 2 0.0952 Tier II
34 NC_000082.6 16 18060838 - Dgcr6 NNNGAAATCATGGACCTTGG NGG 2 0.0833 Tier II
35 NC_000070.6 4 48193881 - Erp44 NNNGAAAGCAGGGACCCTGG NGG 2 0.0706 Tier II
36 NC_000068.7 2 93234446 - Tspan18 NNNGAAAGCATGGGCCTTGG NGG 2 0.0622 Tier II
37 NC_000079.6 13 12046408 - Ryr2 NNNGAAAGCTTGGACCATGG NGA 2 0.0613 Tier II
38 NC_000067.6 1 54783096 + Ankrd44 NNNGAAAGCATGGACCATGT NGA 2 0.0486 Tier II
39 NC_000075.6 9 80396028 + Impg1 NNNGAAAGCATGGACCATGT NGA 2 0.0486 Tier II
40 NC_000086.7 X 102566780 + Phka1 NNNGAAAGGATGGACCATGG NGA 2 0.043 Tier II
41 NC_000068.7 2 153136679 - Hck NNNGAAAGCATGGAGCACGG NGG 2 0.0214 Tier II
42 NC_000085.6 19 27422578 + Pum3 NNNGAAAGCATGGAGCATGC NGG 2 0.0214 Tier II
43 NC_000081.6 15 37610536 - Ncald NNNGAAAGCATGGTCCATGG NTG 2 0.0208 Tier II
44 NC_000080.6 14 76492672 - Tsc22d1 NNNGACAGCATGGACCATGG NTG 2 0.0177 Tier II
45 NC_000069.6 3 103947212 - Rsbn1 NNNGAAAGCATGGCTCATGG NGG 2 0.0143 Tier II
46 NC_000071.6 5 90961446 + Mthfd2l NNNGAAAGCCTGGACCATGG NTG 2 0.013 Tier II
47 NC_000071.6 5 143204356 - Spdye4b NNNGAAAGCATGGAGCCTGG NGG 2 0.0088 Tier II
48 NC_000071.6 5 143217007 + Spdye4a NNNGAAAGCATGGAGCCTGG NGG 2 0.0088 Tier II
49 NC_000069.6 3 83179668 - Dchs2 NNNGAAAGCATGGACGATGG NGT 2 0.0025 Tier II
50 NC_000073.6 7 126230797 + 1700123J17Rik NNNGAAAGCATGGACCATGG NGG 0 1.0 Tier III
51 NC_000074.6 8 50266906 - 1700019L22Rik NNNGAACACATGGACCATGG NGG 2 0.4375 Tier III
52 NC_000072.6 6 129990217 - Gm15854 NNNGAAAGGATGGACCATGT NGG 2 0.4333 Tier III
53 NC_000080.6 14 52571387 + Tcra NNNGAAAGGATGGACCATGT NGG 2 0.4333 Tier III
54 NC_000073.6 7 123085596 + Gm39084 NNNGAAATGATGGACCATGG NGG 2 0.3869 Tier III
55 NC_000071.6 5 120930686 + Gm7573 NNNAAAAGCATGCACCATGG NGG 2 0.3789 Tier III
56 NC_000081.6 15 52845594 + Gm41323 NNNGACAGCATGGACCATAG NGG 2 0.3247 Tier III
57 NC_000081.6 15 79016551 - Gm17753 NNNGACATCATGGACCATGG NGG 2 0.2841 Tier III
58 NC_000076.6 10 22286084 + Gm26740 NNNGAAAGCCTGGACCATTG NGG 2 0.2222 Tier III
59 NC_000076.6 10 84037796 - Gm34184 NNNGACAGCAGGGACCATGG NGG 2 0.1818 Tier III
60 NC_000073.6 7 68861992 - Gm34664 NNNGAAAGCAGTGACCATGG NGG 2 0.1538 Tier III
61 NC_000068.7 2 93234446 - Gm32824 NNNGAAAGCATGGGCCTTGG NGG 2 0.0622 Tier III
62 NC_000072.6 6 82840626 + Gm32591 NNNGAAAGTATGGACCATGG NGA 2 0.0608 Tier III
63 NC_000080.6 14 51332001 - 4930597G03Rik NNNGAAAGCATGGAGAATGG NGG 2 0.05 Tier III
64 NC_000072.6 6 69526123 - Igk NNNGAAAGCATGGACCATGT NGA 2 0.0486 Tier III
65 NC_000071.6 5 109603224 + Vmn2r-ps23 NNNGAAAGCATGGAACATGG NGC 2 0.0049 Tier III
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Other clones with same target sequence:

(none)