Construct: sgRNA BRDN0001145947

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
AGCAGTGTGAAAATGGTGCA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SCYL2 (55681)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76050

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000012.12 12 100311088 + SCYL2 NNNAGTGTGAAAATGGTGCA NGG 0 1.0 Tier I
2 NC_000002.12 2 201412789 - TRAK2 NNNAGTGTGAAAATGGTACA NGG 1 0.6923 Tier II
3 NC_000011.10 11 43439976 + TTC17 NNNAGTGTGAAAATGGTGCT NGG 1 0.6 Tier II
4 NC_000017.11 17 76938163 - MGAT5B NNNAGTGTGAGAATGGTGCG NGG 2 0.4971 Tier II
5 NC_000010.11 10 98507583 - HPSE2 NNNAGTCTGAAAATGGTACA NGG 2 0.476 Tier II
6 NC_000022.11 22 19475350 - UFD1 NNNACTGTGAAAATGGTGCT NGG 2 0.4714 Tier II
7 NC_000001.11 1 172358895 + DNM3 NNNACTGTGGAAATGGTGCA NGG 2 0.4365 Tier II
8 NC_000009.12 9 120689500 + MEGF9 NNNAGTGTGAAAATGGACCA NGG 2 0.254 Tier II
9 NC_000001.11 1 167773981 + MPZL1 NNNAGTGTGATGATGGTGCA NGG 2 0.2222 Tier II
10 NC_000004.12 4 88733209 + FAM13A NNNAGTGTGCAAATGGTTCA NGG 2 0.2222 Tier II
11 NC_000001.11 1 53642368 - GLIS1 NNNAGTGTGAAATAGGTGCA NGG 2 0.1857 Tier II
12 NC_000015.10 15 33055481 + FMN1 NNNAGTGTGAAACTGGTACA NGG 2 0.1457 Tier II
13 NC_000010.11 10 82908045 + NRG3 NNNAGTGTCAAAATGGTGCC NGG 2 0.1224 Tier II
14 NC_000004.12 4 141008771 - RNF150 NNNAGTGTGATATTGGTGCA NGG 2 0.0923 Tier II
15 NC_000007.14 7 139922982 - TBXAS1 NNNAGTGTGATATTGGTGCA NGG 2 0.0923 Tier II
16 NC_000012.12 12 51430065 + SLC4A8 NNNAGGGTGAAAATGGTGGA NGG 2 0.0714 Tier II
17 NC_000023.11 X 7140234 - PUDP NNNAGTGTTAAAATGGTGGA NGG 2 0.0667 Tier II
18 NC_000013.11 13 32038454 - FRY NNNAGTGTGCAAATGGTGGA NGG 2 0.0417 Tier II
19 NC_000019.10 19 38923554 + SARS2 NNNAGTGTGAAAATGGGGCC NGG 2 0.0267 Tier II
20 NC_000008.11 8 98360300 - STK3 NNNAGTGTGCAAATGGTGCA NGA 2 0.0231 Tier II
21 NC_000006.12 6 39914680 - MOCS1 NNNAGTCTGAAAATGCTGCA NGG 2 0.0 Tier II
22 NC_000001.11 1 174228818 + RABGAP1L NNNAGTCTGAAAATGTTGCA NGG 2 0.0 Tier II
23 NC_000009.12 9 4367278 - GLIS3 NNNAGTGTGAAAATGCTGAA NGG 2 0.0 Tier II
24 NC_000001.11 1 154048040 - NUP210L NNNAGTGTGAAAATGTTCCA NGG 2 0.0 Tier II
25 NC_000003.12 3 131947076 - CPNE4 NNNAGTGTGAAATTGTTGCA NGG 2 0.0 Tier II
26 NC_000009.12 9 97934756 - HEMGN NNNAGTGTTAAAATGTTGCA NGG 2 0.0 Tier II
27 NC_000002.12 2 113339507 + LINC02966 NNNACTGTGAAAATAGTGCA NGG 2 0.7395 Tier III
28 NC_000002.12 2 201412789 - SCYL2P1 NNNAGTGTGAAAATGGTACA NGG 1 0.6923 Tier III
29 NC_000001.11 1 165564285 - LRRC52-AS1 NNNAGTGTGAAGATGGTACA NGG 2 0.5 Tier III
30 NC_000010.11 10 129813656 + LOC107984281 NNNTGTGTTAAAATGGTGCA NGG 2 0.3394 Tier III
31 NC_000012.12 12 74232312 + LINC02882 NNNTGTGTGAAAATGGTGAA NGG 2 0.2727 Tier III
32 NC_000012.12 12 48301460 - LOC105369753 NNNAGTGAGAAAATGGTGCA NAG 2 0.2074 Tier III
33 NC_000004.12 4 23069418 + LOC105374524 NNNAGTGTGAAAATTGTGCA NGG 1 0.1429 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000076.6 10 89662422 - Scyl2 NNNAGTGTAAAAATGGTTCA NGG 2 0.4286 Tier I
2 NC_000081.6 15 94283833 - Adamts20 NNNAGTGGGAAAATGGAGCA NGG 2 0.3911 Tier I
3 NC_000082.6 16 11601862 + Snx29 NNNAGTGCTAAAATGGTGCA NGG 2 0.5333 Tier II
4 NC_000079.6 13 73474017 + Lpcat1 NNNAATGTGAAAATGGTGCT NGG 2 0.52 Tier II
5 NC_000072.6 6 58650704 - Abcg2 NNNAATGTGAAAATGGTGAA NGG 2 0.3714 Tier II
6 NC_000081.6 15 65892733 + Oc90 NNNAGTGTAAAAATGGTCCA NGG 2 0.3061 Tier II
7 NC_000074.6 8 119075256 + Cdh13 NNNAGTGTGAAAATGGACCA NGG 2 0.254 Tier II
8 NC_000080.6 14 30853014 + Stimate NNNAGTGTGAAATTGGTTCA NGG 2 0.2 Tier II
9 NC_000075.6 9 76290870 - Hcrtr2 NNNATTGTGAAAATGGAGCA NGG 2 0.16 Tier II
10 NC_000067.6 1 25652375 - Adgrb3 NNNAGTGTGAAAATGGAGCA NAG 2 0.1383 Tier II
11 NC_000068.7 2 106135686 - Dcdc5 NNNAGTGTGATCATGGTGCA NGG 2 0.081 Tier II
12 NC_000068.7 2 164484483 + Dbndd2 NNNAGTTTGAAAATGGGGCA NGG 2 0.0672 Tier II
13 NC_000086.7 X 7739747 - Ccdc120 NNNAGTGTGAAAATGGGGAA NGG 2 0.0504 Tier II
14 NC_000067.6 1 180948966 + Tmem63a NNNAGTGTGAATATTGTGCA NGG 2 0.0476 Tier II
15 NC_000086.7 X 113631040 - Dach2 NNNAGTGTGAAAATGATGCA NGT 2 0.0161 Tier II
16 NC_000071.6 5 131987183 + Auts2 NNNAGTGTTAAAATGGTGCA NGT 2 0.0086 Tier II
17 NC_000069.6 3 60020822 - Aadacl2 NNNAGTGTGAAATTGGTGCA NGT 2 0.0048 Tier II
18 NC_000068.7 2 120304514 + Pla2g4f NNNAGTGTGAAAAGGGAGCA NGG 2 0.0 Tier II
19 NC_000067.6 1 152583526 - Rgl1 NNNAGTGTGTAAAGGGTGCA NGG 2 0.0 Tier II
20 NC_000082.6 16 5403788 - Gm41414 NNNACTGTGTAAATGGTGCA NGG 2 0.6933 Tier III
21 NC_000069.6 3 143480882 - Gm33604 NNNAGTGTGAAAATGGTAAA NGG 2 0.2967 Tier III
22 NC_000074.6 8 90739578 - Gm35850 NNNAGTGTGAAAATGGAGCA NAG 2 0.1383 Tier III
23 NC_000069.6 3 60020822 - Gm18427 NNNAGTGTGAAATTGGTGCA NGT 2 0.0048 Tier III
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Other clones with same target sequence:

(none)