Construct: sgRNA BRDN0001145958

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
TTCTGTCACCACATCTGTCA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PAK1 (5058)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76464

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000011.10 11 77340705 + PAK1 NNNTGTCACCACATCTGTCA NGG 0 1.0 Tier I
2 NC_000015.10 15 73376227 - LOC124903571 NNNTGTCACCACATCTGTCA NAG 1 0.2593 Tier I
3 NC_000012.12 12 56181712 + SMARCC2 NNNTGTCACCTCATCTGTCA NTG 2 0.012 Tier I
4 NC_000011.10 11 78897611 + TENM4 NNNTGTCACAACATCAGTCA NGG 2 0.7879 Tier II
5 NC_000006.12 6 65393698 - EYS NNNTCTCACCACATCAGTCA NGG 2 0.7143 Tier II
6 NC_000005.10 5 77590746 - WDR41 NNNTGCCACGACATCTGTCA NGG 2 0.3535 Tier II
7 NC_000009.12 9 117356359 - ASTN2 NNNTCTCACCACATATGTCA NGG 2 0.1746 Tier II
8 NC_000019.10 19 40442645 + SERTAD3 NNNTCTCACCACCTCTGTCA NGG 2 0.1654 Tier II
9 NC_000011.10 11 131418413 + NTM NNNTGTCGCCACATCTGTGA NGG 2 0.0917 Tier II
10 NC_000009.12 9 27572302 - C9orf72 NNNTGTCACCACCTCTGCCA NGG 2 0.0902 Tier II
11 NC_000019.10 19 15924485 + CYP4F11 NNNTGTGACCACATCTGTCA NCG 2 0.0504 Tier II
12 NC_000018.10 18 3415083 + TGIF1 NNNTGTCACCACCTCTGTCC NGG 2 0.0478 Tier II
13 NC_000006.12 6 64270291 + EYS NNNTGTCATCACATGTGTCA NGG 2 0.0438 Tier II
14 NC_000001.11 1 206536450 + RASSF5 NNNTGTCCCCACATTTGTCA NGG 2 0.0286 Tier II
15 NC_000016.10 16 12157919 + SNX29 NNNTGTCACCACCTCTGTGA NGG 2 0.0263 Tier II
16 NC_000005.10 5 59847038 - PDE4D NNNTGTCACCACCTCTGTCA NCG 2 0.0226 Tier II
17 NC_000023.11 X 131068956 + ARHGAP36 NNNTGTCACCACATTTGGCA NGG 2 0.0222 Tier II
18 NC_000016.10 16 12092849 - SNX29 NNNTGTCACCACATGTGTAA NGG 2 0.0214 Tier II
19 NC_000009.12 9 15173443 + TTC39B NNNTGTCACCTCATTTGTCA NGG 2 0.0205 Tier II
20 NC_000023.11 X 14011692 - GEMIN8 NNNTGTCACCAGATCTGTCA NTG 2 0.0173 Tier II
21 NC_000002.12 2 98639719 + MGAT4A NNNTGTCCCCACATCTGTCA NGC 2 0.0095 Tier II
22 NC_000015.10 15 65929507 + MEGF11 NNNTGTCACCACATCTGCCA NGT 2 0.0069 Tier II
23 NC_000006.12 6 163123302 - PACRG NNNTGTCACCACAGCTGTCA NGG 1 0.0 Tier II
24 NC_000005.10 5 170782177 + LOC107986475 NNNTGTTACCACATCTGTCA NTG 2 0.0317 Tier III
25 NC_000005.10 5 59847038 - LOC107986350 NNNTGTCACCACCTCTGTCA NCG 2 0.0226 Tier III
26 NC_000017.11 17 30780636 + SUZ12P1 NNNTGTCATCACATCTGTCA NGC 2 0.0194 Tier III
27 NC_000009.12 9 1733307 + LOC105375951 NNNTGTGACCACAGCTGTCA NGG 2 0.0 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000073.6 7 97900399 - Pak1 NNNTGTCACCACATCTGTCA NGG 0 1.0 Tier I
2 NC_000085.6 19 7603058 + Lgals12 NNNTGTCACCAGATATGTCA NGG 2 0.0988 Tier I
3 NC_000073.6 7 100540019 + Coa4 NNNTGACACCACATCTGTCA NGG 1 0.8667 Tier II
4 NC_000085.6 19 53236114 - Add3 NNNTGAAACCACATCTGTCA NGG 2 0.65 Tier II
5 NC_000083.6 17 75516892 + Rasgrp3 NNNTGTCACCACATCCATCA NGG 2 0.6222 Tier II
6 NC_000069.6 3 152196711 + Dnajb4 NNNGGTCACTACATCTGTCA NGG 2 0.5882 Tier II
7 NC_000072.6 6 83176201 + Dctn1 NNNTGGCATCACATCTGTCA NGG 2 0.5 Tier II
8 NC_000070.6 4 154441215 + Prdm16 NNNTGTCACCACATCTATAA NGG 2 0.4 Tier II
9 NC_000076.6 10 79821705 + Misp NNNGGGCACCACATCTGTCA NGG 2 0.3571 Tier II
10 NC_000077.6 11 83774749 - Heatr6 NNNTGTGGCCACATCTGTCA NGG 2 0.3451 Tier II
11 NC_000067.6 1 135734057 + Csrp1 NNNTGTCCCCACATCTGACA NGG 2 0.2857 Tier II
12 NC_000067.6 1 134469314 + Klhl12 NNNTGCCACCACACCTGTCA NGG 2 0.2597 Tier II
13 NC_000082.6 16 27344155 + Ostn NNNTGTCAACACATCTGTCA NAG 2 0.2222 Tier II
14 NC_000082.6 16 35398461 + Pdia5 NNNAGTCACCACATCTGCCA NGG 2 0.2041 Tier II
15 NC_000075.6 9 49100668 - Tmprss5 NNNTGTCACCACATCTGGCT NGG 2 0.2 Tier II
16 NC_000077.6 11 98746161 + Thra NNNTGTCACCACATCTCTCT NGG 2 0.1412 Tier II
17 NC_000086.7 X 169002730 + Arhgap6 NNNTTTCACCACATCTGTCC NGG 2 0.0682 Tier II
18 NC_000067.6 1 21751971 + Kcnq5 NNNTCTCACCACATCTGTCA NGA 2 0.0546 Tier II
19 NC_000075.6 9 123039999 - Tgm4 NNNTGTCACTACATCTGTCA NTG 2 0.0367 Tier II
20 NC_000082.6 16 38098655 + Gsk3b NNNTGTTACCACATCTGTCA NTG 2 0.0317 Tier II
21 NC_000075.6 9 119426498 + Acvr2b NNNTGTCACCACAAGTGTCA NGG 2 0.031 Tier II
22 NC_000076.6 10 18712497 - Arfgef3 NNNTGTCACCACATCTGTAA NGA 2 0.0298 Tier II
23 NC_000071.6 5 97078561 - Bmp2k NNNTGTCACCACATCTGACA NTG 2 0.026 Tier II
24 NC_000084.6 18 5689201 - Zeb1 NNNTATCACCACATCTGTCA NGT 2 0.014 Tier II
25 NC_000083.6 17 26623926 - Ergic1 NNNTGTGACCACATCTGTCA NGT 2 0.0076 Tier II
26 NC_000073.6 7 75523571 + Akap13 NNNTTTCACCACATCTGTCA NGT 2 0.0048 Tier II
27 NC_000067.6 1 193107396 - Utp25 NNNGGTCACCACAGCTGTCA NGG 2 0.0 Tier II
28 NC_000067.6 1 150087824 - Ptgs2os NNNTGTCACTGCATCTGTCA NGG 2 0.6118 Tier III
29 NC_000069.6 3 60823745 - Gm553061 NNNTGCCACCACATCTGGCA NGG 2 0.303 Tier III
30 NC_000085.6 19 60069961 + Csf1r-ps NNNTGTCTCCACATCTTTCA NGG 2 0.1071 Tier III
31 NC_000078.6 12 113895557 - Igh NNNTGTCACCACATGTGTAA NGG 2 0.0214 Tier III
32 NC_000068.7 2 124758779 + Gm39920 NNNTGTCACCACATCTGGCA NGC 2 0.0074 Tier III
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Other clones with same target sequence:

(none)