Construct: sgRNA BRDN0001146005

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
CAAAGAAGGCCTTTGTACAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
AGK (55750)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76154

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000007.14 7 141641330 - AGK NNNAGAAGGCCTTTGTACAG NGG 0 1.0 Tier I
2 NC_000010.11 10 132222776 + STK32C NNNAGACGGCCTTTCTACAG NGG 2 0.1193 Tier I
3 NC_000016.10 16 53465478 + RBL2 NNNAGATGGCCTTTGTAGAG NGG 2 0.0583 Tier I
4 NC_000024.10 Y 14639904 - NLGN4Y NNNAGAAGGCCTTTGCACAG NGG 1 0.6667 Tier II
5 NC_000014.9 14 51474768 + FRMD6 NNNAGAAGGCCTTTGAACAT NGG 2 0.6364 Tier II
6 NC_000023.11 X 63758451 - ARHGEF9 NNNTGAAGGCCTTTGAACAG NGG 2 0.5785 Tier II
7 NC_000003.12 3 172171041 - FNDC3B NNNAGAAGGCCTTTGTATAT NGG 2 0.45 Tier II
8 NC_000003.12 3 155648459 - PLCH1 NNNAGTAGGCCTTTGTAAAG NGG 2 0.3846 Tier II
9 NC_000016.10 16 3645303 + DNASE1 NNNAGAAGGCCTTTGTACTT NGG 2 0.3769 Tier II
10 NC_000017.11 17 45300637 - MAP3K14 NNNAGGAGGCCGTTGTACAG NGG 2 0.3571 Tier II
11 NC_000011.10 11 44882403 + TSPAN18 NNNAGCAGGGCTTTGTACAG NGG 2 0.1768 Tier II
12 NC_000015.10 15 31088832 + TRPM1 NNNAGGAGGCCTTTGTACCG NGG 2 0.1478 Tier II
13 NC_000017.11 17 6072091 - WSCD1 NNNAGAAGGCATTTTTACAG NGG 2 0.1071 Tier II
14 NC_000010.11 10 15804424 + MINDY3 NNNATAAGGCCTGTGTACAG NGG 2 0.0783 Tier II
15 NC_000010.11 10 48803106 + WDFY4 NNNAGAAGGCCTTCCTACAG NGG 2 0.0779 Tier II
16 NC_000001.11 1 121231441 - SRGAP2C NNNAGAAGTCCTTTTTACAG NGG 2 0.0762 Tier II
17 NC_000001.11 1 145048911 + SRGAP2B NNNAGAAGTCCTTTTTACAG NGG 2 0.0762 Tier II
18 NC_000001.11 1 206249998 - SRGAP2 NNNAGAAGTCCTTTTTACAG NGG 2 0.0762 Tier II
19 NC_000015.10 15 90988490 - PRC1 NNNAGAAGGCCTTTCTACAG NAG 2 0.0707 Tier II
20 NC_000013.11 13 25400870 + ATP8A2 NNNAGAAGGCCTTGGGACAG NGG 2 0.0 Tier II
21 NC_000024.10 Y 14639904 - AGKP1 NNNAGAAGGCCTTTGCACAG NGG 1 0.6667 Tier III
22 NC_000014.9 14 51474768 + FRMD6-AS2 NNNAGAAGGCCTTTGAACAT NGG 2 0.6364 Tier III
23 NC_000003.12 3 172171041 - LOC124906304 NNNAGAAGGCCTTTGTATAT NGG 2 0.45 Tier III
24 NC_000023.11 X 132130544 + AGKP2 NNNAGAAGGCCTTCGTACAG NGG 1 0.2857 Tier III
25 NC_000009.12 9 31897903 - LOC105376011 NNNAGAAGTCTTTTGTACAG NGG 2 0.1641 Tier III
26 NC_000009.12 9 31897903 - LOC124902137 NNNAGAAGTCTTTTGTACAG NGG 2 0.1641 Tier III
27 NC_000005.10 5 160468526 + MIR3142HG NNNAGCAGGCTTTTGTACAG NGG 2 0.1399 Tier III
28 NC_000010.11 10 90525969 - LINC02653 NNNGGAAGGCCTTTGTCCAG NGG 2 0.1103 Tier III
29 NC_000010.11 10 15804424 + LOC124902383 NNNATAAGGCCTGTGTACAG NGG 2 0.0783 Tier III
30 NC_000001.11 1 144019044 - SRGAP2D NNNAGAAGTCCTTTTTACAG NGG 2 0.0762 Tier III
31 NC_000018.10 18 54532066 - DYNAPP1 NNNAGAAGCCCTTTGTTCAG NGG 2 0.0718 Tier III
32 NC_000015.10 15 90988490 - PRC1-AS1 NNNAGAAGGCCTTTCTACAG NAG 2 0.0707 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000081.6 15 54274461 + Tnfrsf11b NNNAGAAAGCCTTTATACAG NGG 2 0.9412 Tier II
2 NC_000074.6 8 111566847 + Znrf1 NNNAGGAGGCCTTTGTACAT NGG 2 0.5 Tier II
3 NC_000071.6 5 3346877 + Cdk6 NNNAGAGGGCCTATGTACAG NGG 2 0.4887 Tier II
4 NC_000072.6 6 142244740 + Slco1a5 NNNAGGATGCCTTTGTACAG NGG 2 0.4464 Tier II
5 NC_000070.6 4 103817137 + Dab1 NNNGGAGGGCCTTTGTACAG NGG 2 0.4412 Tier II
6 NC_000083.6 17 25435613 - Cacna1h NNNAGAAGGCCTTTGCATAG NGG 2 0.4286 Tier II
7 NC_000081.6 15 6553206 - Fyb NNNAGCAGGCCTTTGAACAG NGG 2 0.4132 Tier II
8 NC_000074.6 8 14353643 - Dlgap2 NNNTGAAGACCTTTGTACAG NGG 2 0.4091 Tier II
9 NC_000074.6 8 41296349 + Pcm1 NNNTGAAGTCCTTTGTACAG NGG 2 0.3394 Tier II
10 NC_000074.6 8 126181208 - Slc35f3 NNNATAAGGCCTTTGAACAG NGG 2 0.2727 Tier II
11 NC_000067.6 1 36340500 - Kansl3 NNNACAAGGCCTTTGTACAG NAG 2 0.2037 Tier II
12 NC_000072.6 6 33347972 + Exoc4 NNNGTAAGGCCTTTGTACAG NGG 2 0.1875 Tier II
13 NC_000070.6 4 59648821 + E130308A19Rik NNNGGAAGGCCTTTCTACAG NGG 2 0.1705 Tier II
14 NC_000072.6 6 115806855 - Rpl32 NNNAGAAGGTCTTTTTACAG NGG 2 0.1345 Tier II
15 NC_000070.6 4 134539085 + Selenon NNNAGAAGGGCTTTGTGCAG NGG 2 0.0686 Tier II
16 NC_000070.6 4 32397938 + Bach2 NNNAGAAGGGCTTTTTACAG NGG 2 0.0556 Tier II
17 NC_000070.6 4 104112534 + Dab1 NNNAGAAGGCCTTTGGAGAG NGG 2 0.0462 Tier II
18 NC_000075.6 9 76516205 - Fam83b NNNGGAAGGCCTTTGTACAG NGA 2 0.0434 Tier II
19 NC_000076.6 10 64447711 - Ctnna3 NNNAGAAGGCCTTTGTAAAG NTG 2 0.021 Tier II
20 NC_000075.6 9 32783836 - Gm3331 NNNAGAAGGCAGTTGTACAG NGG 2 0.375 Tier III
21 NC_000074.6 8 72281613 + Gm51537 NNNTGAAGGCCTTTCTACAG NGG 2 0.1736 Tier III
22 NC_000067.6 1 73843738 + 6030407O03Rik NNNAGAAGGCCTTTGTCCAG NAG 2 0.0458 Tier III
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Other clones with same target sequence:

(none)