Construct: sgRNA BRDN0001146028
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GGTACAATGTGAAAGGCCGA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- IGF1R (3480)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76689
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000015.10 |
15 |
98922328 |
- |
IGF1R |
NNNACAATGTGAAAGGCCGA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000016.10 |
16 |
65030988 |
+ |
CDH11 |
NNNACAATGTAAAAGGCCTA |
NGG |
2 |
0.6667 |
Tier II |
| 3 |
NC_000004.12 |
4 |
53266842 |
- |
SCFD2 |
NNNACAATTTGAAAAGCCGA |
NGG |
2 |
0.502 |
Tier II |
| 4 |
NC_000009.12 |
9 |
8695084 |
- |
PTPRD |
NNNACAATGAGAAAGGCAGA |
NGG |
2 |
0.4615 |
Tier II |
| 5 |
NC_000020.11 |
20 |
42214554 |
+ |
PTPRT |
NNNACAATTTGAAAGGCCAA |
NGG |
2 |
0.381 |
Tier II |
| 6 |
NC_000012.12 |
12 |
129722606 |
- |
TMEM132D |
NNNACATTGAGAAAGGCCGA |
NGG |
2 |
0.375 |
Tier II |
| 7 |
NC_000004.12 |
4 |
94660812 |
+ |
PDLIM5 |
NNNACAATGTCAAAGGCCAA |
NGG |
2 |
0.3061 |
Tier II |
| 8 |
NC_000023.11 |
X |
6875987 |
- |
PUDP |
NNNACAATTTGAAAGGCAGA |
NGG |
2 |
0.2872 |
Tier II |
| 9 |
NC_000010.11 |
10 |
116839956 |
+ |
HSPA12A |
NNNAGAATGTGAAAGGCCCA |
NGG |
2 |
0.269 |
Tier II |
| 10 |
NC_000014.9 |
14 |
64032217 |
+ |
SYNE2 |
NNNACAATGTGAAATGCCTA |
NGG |
2 |
0.0952 |
Tier II |
| 11 |
NC_000001.11 |
1 |
47267470 |
+ |
STIL |
NNNACAATTTGAAAGGCCGA |
NGC |
2 |
0.0119 |
Tier II |
| 12 |
NC_000017.11 |
17 |
83188005 |
+ |
LOC101929650 |
NNNACTATGTGAAAGGCCTA |
NGG |
2 |
0.4762 |
Tier III |
| 13 |
NC_000002.12 |
2 |
220795368 |
- |
LOC105373896 |
NNNACAAAGTGAAAGGCAGA |
NGG |
2 |
0.4308 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000067.6 |
1 |
194762033 |
+ |
Plxna2 |
NNNACAATGTAAAAGGCCAA |
NGG |
2 |
0.7143 |
Tier II |
| 2 |
NC_000083.6 |
17 |
88642534 |
+ |
Ston1 |
NNNACAATGTGAGAGGCCCA |
NGG |
2 |
0.2924 |
Tier II |
| 3 |
NC_000079.6 |
13 |
90978738 |
+ |
Atg10 |
NNNAAAATGTGAAAGGACGA |
NGG |
2 |
0.2667 |
Tier II |
| 4 |
NC_000078.6 |
12 |
34881262 |
- |
Hdac9 |
NNNACAATGTGAAAGGAGGA |
NGG |
2 |
0.0622 |
Tier II |
| 5 |
NC_000076.6 |
10 |
49429166 |
+ |
Grik2 |
NNNACAATGTGAAAGGCCCA |
NGT |
2 |
0.0072 |
Tier II |
| 6 |
NC_000078.6 |
12 |
98107172 |
+ |
Gm47415 |
NNNACAATGTGAAAGGCTGA |
NAG |
2 |
0.1667 |
Tier III |
| 7 |
NC_000078.6 |
12 |
34881262 |
- |
Gm40875 |
NNNACAATGTGAAAGGAGGA |
NGG |
2 |
0.0622 |
Tier III |
Other clones with same target sequence:
(none)
