Construct: sgRNA BRDN0001146035

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
AGTGCACAGAAGGCTCCATG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ALPK1 (80216)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76081

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000004.12 4 112430653 + ALPK1 NNNGCACAGAAGGCTCCATG NGG 0 1.0 Tier I
2 NC_000002.12 2 10170777 + RRM2 NNNGCACAGGAGACTCCATG NGG 2 0.5128 Tier II
3 NC_000005.10 5 139148450 + SIL1 NNNCCACAGTAGGCTCCATG NGG 2 0.4671 Tier II
4 NC_000011.10 11 132942844 - OPCML NNNGGGCAGAAGGCTCCATG NGG 2 0.4286 Tier II
5 NC_000010.11 10 115537865 - ATRNL1 NNNGCAAAGAAGGCTCTATG NGG 2 0.35 Tier II
6 NC_000007.14 7 135246174 + STRA8 NNNACACAGAAGGCTTCATG NGG 2 0.2769 Tier II
7 NC_000008.11 8 142531109 + ADGRB1 NNNGCAAAGAAGTCTCCATG NGG 2 0.225 Tier II
8 NC_000022.11 22 29410938 - RFPL1 NNNGAACAGCAGGCTCCATG NGG 2 0.1905 Tier II
9 NC_000006.12 6 111368296 - REV3L NNNGGACAGAAGGCTTCATG NGG 2 0.1846 Tier II
10 NC_000013.11 13 51935459 + ATP7B NNNGCACACCAGGCTCCATG NGG 2 0.1795 Tier II
11 NC_000015.10 15 91150878 + SV2B NNNGAACAGATGGCTCCATG NGG 2 0.1758 Tier II
12 NC_000002.12 2 237771751 + LRRFIP1 NNNGCCCAGAAGGCTCCAAG NGG 2 0.1299 Tier II
13 NC_000019.10 19 38131339 + SIPA1L3 NNNGCAGAGAAGGCTCCACG NGG 2 0.1298 Tier II
14 NC_000006.12 6 5063265 - LYRM4 NNNGCACAGAATGCTTCATG NGG 2 0.1183 Tier II
15 NC_000002.12 2 54374877 + CIMIP6 NNNGCACAGAAGGCTCCGTG NAG 2 0.1037 Tier II
16 NC_000008.11 8 144055968 + OPLAH NNNGCACAGAGGGCTGCATG NGG 2 0.1 Tier II
17 NC_000012.12 12 44044630 + TMEM117 NNNGCACAGATGGCTTCATG NGG 2 0.0947 Tier II
18 NC_000002.12 2 205498805 - PARD3B NNNTCACAGAAGGCTCCATG NAG 2 0.0943 Tier II
19 NC_000012.12 12 6731108 - COPS7A NNNGCACAGAAGGCTCTCTG NGG 2 0.0889 Tier II
20 NC_000012.12 12 113389484 - PLBD2 NNNGCACAGGAGGCTGCATG NGG 2 0.0855 Tier II
21 NC_000020.11 20 62249964 - OSBPL2 NNNGCACAGATGGCCCCATG NGG 2 0.0839 Tier II
22 NC_000010.11 10 129540892 - MGMT NNNGCACAGATGGCTCCAGG NGG 2 0.0769 Tier II
23 NC_000007.14 7 1064877 + CHLSN NNNGCACAGAAGGCCCCAGG NGG 2 0.0682 Tier II
24 NC_000001.11 1 201592633 + NAV1 NNNGCACAGAAGGCTTCCTG NGG 2 0.0586 Tier II
25 NC_000003.12 3 17196320 + TBC1D5 NNNGCAGAGAAGGCTCCATG NGA 2 0.0327 Tier II
26 NC_000014.9 14 72792000 + DPF3 NNNGCACAGAGGGCTCCATG NTG 2 0.0253 Tier II
27 NC_000010.11 10 116816538 + HSPA12A NNNGCAGAGAAGGCGCCATG NGG 2 0.0235 Tier II
28 NC_000012.12 12 97200606 - LOC105369927 NNNGCACAAAGGGCTCCATG NGG 2 0.4179 Tier III
29 NC_000012.12 12 97200606 - LOC105369928 NNNGCACAAAGGGCTCCATG NGG 2 0.4179 Tier III
30 NC_000020.11 20 58579960 - APCDD1L-DT NNNCCTCAGAAGGCTCCATG NGG 2 0.3782 Tier III
31 NC_000023.11 X 129015401 + LOC124905213 NNNGTACAGAAGGCTCCTTG NGG 2 0.3182 Tier III
32 NC_000001.11 1 8140088 + LOC107984915 NNNGCACAAAAGGCTCTATG NGG 2 0.3 Tier III
33 NC_000009.12 9 86458021 + LOC102724080 NNNGCACAAAAGGCTCTATG NGG 2 0.3 Tier III
34 NC_000002.12 2 22541355 + LINC01830 NNNGCACAGAAGACTCCATG NAG 2 0.2393 Tier III
35 NC_000022.11 22 29410938 - LOC102723305 NNNGAACAGCAGGCTCCATG NGG 2 0.1905 Tier III
36 NC_000010.11 10 24756429 - LOC105376456 NNNGCACATAAGGCTTCATG NGG 2 0.1641 Tier III
37 NC_000008.11 8 129370627 + CCDC26 NNNTCACAGAAGGCTCCATC NGG 2 0.1558 Tier III
38 NC_000006.12 6 5063265 - LYRM4-AS1 NNNGCACAGAATGCTTCATG NGG 2 0.1183 Tier III
39 NC_000017.11 17 74104663 + LINC02074 NNNGCACAGAAGGCCCCATG NTG 2 0.0106 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000067.6 1 80213066 + Fam124b NNNGCACAGAAGACTCCTTG NGG 2 0.4615 Tier I
2 NC_000086.7 X 164108415 - Cltrn NNNGCACAGATGGCTCCATC NGG 2 0.1319 Tier I
3 NC_000073.6 7 127934615 + Prss36 NNNGCACAGAAGGCTCCAAC NGG 2 0.1224 Tier I
4 NC_000074.6 8 32412577 - Nrg1 NNNGCAAAGAAGACTCCATG NGG 2 0.6923 Tier II
5 NC_000074.6 8 124250881 - Galnt2 NNNGCACAGGAGGCTCCATG NGG 1 0.5556 Tier II
6 NC_000072.6 6 6065630 - Slc25a13 NNNAAACAGAAGGCTCCATG NGG 2 0.5143 Tier II
7 NC_000077.6 11 118625134 - Rbfox3 NNNAAACAGAAGGCTCCATG NGG 2 0.5143 Tier II
8 NC_000067.6 1 156920688 + Ralgps2 NNNGCACAGAAGGCTAAATG NGG 2 0.4667 Tier II
9 NC_000077.6 11 67052931 - Sco1 NNNGCCCAGAAGGCTCCATG NGG 1 0.4545 Tier II
10 NC_000067.6 1 105801709 - Tnfrsf11a NNNCCACGGAAGGCTCCATG NGG 2 0.3882 Tier II
11 NC_000076.6 10 123670781 + Tafa2 NNNACACAGAAGGCTCCATC NGG 2 0.3857 Tier II
12 NC_000072.6 6 52788450 + Jazf1 NNNGGACAAAAGGCTCCATG NGG 2 0.3857 Tier II
13 NC_000072.6 6 115888357 + Ift122 NNNGCACAGAGGCCTCCATG NGG 2 0.2737 Tier II
14 NC_000074.6 8 88562605 - Nkd1 NNNGCACACAAGGCTCCTTG NGG 2 0.2692 Tier II
15 NC_000083.6 17 67312074 + Ptprm NNNGCACAGGAGGATCCATG NGG 2 0.1944 Tier II
16 NC_000068.7 2 91987159 + Creb3l1 NNNGCACAGAAGTCACCATG NGG 2 0.1737 Tier II
17 NC_000067.6 1 152414534 - Colgalt2 NNNGCACAGAACGCTCCAAG NGG 2 0.1513 Tier II
18 NC_000079.6 13 108325185 - Depdc1b NNNGCACAGGAGGCTCCAGG NGG 2 0.1389 Tier II
19 NC_000077.6 11 119868767 - Rptor NNNCCACAGAAGGCTCCATG NAG 2 0.1373 Tier II
20 NC_000085.6 19 5723986 + Ehbp1l1 NNNGCAGAGAAGGCTCCAGG NGG 2 0.1176 Tier II
21 NC_000067.6 1 194696373 - Plxna2 NNNGCCCAGAAGGCTCCAGG NGG 2 0.1136 Tier II
22 NC_000074.6 8 70652565 - Pgpep1 NNNGCACAGAAGGCTCCGTG NAG 2 0.1037 Tier II
23 NC_000076.6 10 85421428 + Btbd11 NNNGCACAGAAGGCAGCATG NGG 2 0.0891 Tier II
24 NC_000078.6 12 53773940 - Npas3 NNNGCACAGAAGTCTCCATG NAG 2 0.0778 Tier II
25 NC_000075.6 9 58049236 - Ccdc33 NNNGCACAGAAGGCCCCATG NAG 2 0.0707 Tier II
26 NC_000073.6 7 4491974 - Ppp1r12c NNNGCACAGAAGGATCCCTG NGG 2 0.0667 Tier II
27 NC_000073.6 7 82534454 - Adamtsl3 NNNGCACAGAAGGCTCCAGG NAG 2 0.0648 Tier II
28 NC_000074.6 8 14613638 - Dlgap2 NNNGCACACAAGGCTCCATG NTG 2 0.021 Tier II
29 NC_000074.6 8 122797838 + Acsf3 NNNGCACTGAAGGCTCCATG NTG 2 0.0167 Tier II
30 NC_000074.6 8 40356088 + Micu3 NNNGCACAGAAGCCTCCATG NTG 2 0.0164 Tier II
31 NC_000082.6 16 70337933 - Gbe1 NNNGCACAGAAGGCTCCATT NGC 2 0.0156 Tier II
32 NC_000069.6 3 9516624 - Zfp704 NNNGCACAGAAGCCTCCATG NGC 2 0.0094 Tier II
33 NC_000067.6 1 80213066 + 2310015K22Rik NNNGCACAGAAGACTCCTTG NGG 2 0.4615 Tier III
34 NC_000067.6 1 80213066 + LOC108167342 NNNGCACAGAAGACTCCTTG NGG 2 0.4615 Tier III
35 NC_000072.6 6 52788450 + Gm52904 NNNGGACAAAAGGCTCCATG NGG 2 0.3857 Tier III
36 NC_000077.6 11 112891678 - Gm46301 NNNGAACAGAAGGCTTCATG NGG 2 0.1758 Tier III
37 NC_000067.6 1 194696373 - Gm46202 NNNGCCCAGAAGGCTCCAGG NGG 2 0.1136 Tier III
38 NC_000081.6 15 79022182 - Gm17753 NNNGCACAGAAGGCTCCCTG NAG 2 0.0494 Tier III
39 NC_000067.6 1 105423141 + Gm28403 NNNGCACAGAATGCTCCATG NTG 2 0.015 Tier III
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Other clones with same target sequence:

(none)