Construct: sgRNA BRDN0001146035
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AGTGCACAGAAGGCTCCATG
Original Target:
- Taxon:
- Homo sapiens (human)
- Gene:
- ALPK1 (80216)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
Reference Sequence | Chromosome | Cut Position | Match Strand | Match Gene | Match Sequence | PAM Seq. | Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. | CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. | Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. | |
---|---|---|---|---|---|---|---|---|---|---|
1 | NC_000004.12 | 4 | 112430653 | + | ALPK1 | NNNGCACAGAAGGCTCCATG | NGG | 0 | 1.0 | Tier I |
2 | NC_000002.12 | 2 | 10170777 | + | RRM2 | NNNGCACAGGAGACTCCATG | NGG | 2 | 0.5128 | Tier II |
3 | NC_000005.10 | 5 | 139148450 | + | SIL1 | NNNCCACAGTAGGCTCCATG | NGG | 2 | 0.4671 | Tier II |
4 | NC_000011.10 | 11 | 132942844 | - | OPCML | NNNGGGCAGAAGGCTCCATG | NGG | 2 | 0.4286 | Tier II |
5 | NC_000010.11 | 10 | 115537865 | - | ATRNL1 | NNNGCAAAGAAGGCTCTATG | NGG | 2 | 0.35 | Tier II |
6 | NC_000007.14 | 7 | 135246174 | + | STRA8 | NNNACACAGAAGGCTTCATG | NGG | 2 | 0.2769 | Tier II |
7 | NC_000008.11 | 8 | 142531109 | + | ADGRB1 | NNNGCAAAGAAGTCTCCATG | NGG | 2 | 0.225 | Tier II |
8 | NC_000022.11 | 22 | 29410938 | - | RFPL1 | NNNGAACAGCAGGCTCCATG | NGG | 2 | 0.1905 | Tier II |
9 | NC_000006.12 | 6 | 111368296 | - | REV3L | NNNGGACAGAAGGCTTCATG | NGG | 2 | 0.1846 | Tier II |
10 | NC_000013.11 | 13 | 51935459 | + | ATP7B | NNNGCACACCAGGCTCCATG | NGG | 2 | 0.1795 | Tier II |
11 | NC_000015.10 | 15 | 91150878 | + | SV2B | NNNGAACAGATGGCTCCATG | NGG | 2 | 0.1758 | Tier II |
12 | NC_000002.12 | 2 | 237771751 | + | LRRFIP1 | NNNGCCCAGAAGGCTCCAAG | NGG | 2 | 0.1299 | Tier II |
13 | NC_000019.10 | 19 | 38131339 | + | SIPA1L3 | NNNGCAGAGAAGGCTCCACG | NGG | 2 | 0.1298 | Tier II |
14 | NC_000006.12 | 6 | 5063265 | - | LYRM4 | NNNGCACAGAATGCTTCATG | NGG | 2 | 0.1183 | Tier II |
15 | NC_000002.12 | 2 | 54374877 | + | CIMIP6 | NNNGCACAGAAGGCTCCGTG | NAG | 2 | 0.1037 | Tier II |
16 | NC_000008.11 | 8 | 144055968 | + | OPLAH | NNNGCACAGAGGGCTGCATG | NGG | 2 | 0.1 | Tier II |
17 | NC_000012.12 | 12 | 44044630 | + | TMEM117 | NNNGCACAGATGGCTTCATG | NGG | 2 | 0.0947 | Tier II |
18 | NC_000002.12 | 2 | 205498805 | - | PARD3B | NNNTCACAGAAGGCTCCATG | NAG | 2 | 0.0943 | Tier II |
19 | NC_000012.12 | 12 | 6731108 | - | COPS7A | NNNGCACAGAAGGCTCTCTG | NGG | 2 | 0.0889 | Tier II |
20 | NC_000012.12 | 12 | 113389484 | - | PLBD2 | NNNGCACAGGAGGCTGCATG | NGG | 2 | 0.0855 | Tier II |
21 | NC_000020.11 | 20 | 62249964 | - | OSBPL2 | NNNGCACAGATGGCCCCATG | NGG | 2 | 0.0839 | Tier II |
22 | NC_000010.11 | 10 | 129540892 | - | MGMT | NNNGCACAGATGGCTCCAGG | NGG | 2 | 0.0769 | Tier II |
23 | NC_000007.14 | 7 | 1064877 | + | C7orf50 | NNNGCACAGAAGGCCCCAGG | NGG | 2 | 0.0682 | Tier II |
24 | NC_000001.11 | 1 | 201592633 | + | NAV1 | NNNGCACAGAAGGCTTCCTG | NGG | 2 | 0.0586 | Tier II |
25 | NC_000003.12 | 3 | 17196320 | + | TBC1D5 | NNNGCAGAGAAGGCTCCATG | NGA | 2 | 0.0327 | Tier II |
26 | NC_000014.9 | 14 | 72792000 | + | DPF3 | NNNGCACAGAGGGCTCCATG | NTG | 2 | 0.0253 | Tier II |
27 | NC_000010.11 | 10 | 116816538 | + | HSPA12A | NNNGCAGAGAAGGCGCCATG | NGG | 2 | 0.0235 | Tier II |
28 | NC_000012.12 | 12 | 97200606 | - | LOC105369927 | NNNGCACAAAGGGCTCCATG | NGG | 2 | 0.4179 | Tier III |
29 | NC_000012.12 | 12 | 97200606 | - | LOC105369928 | NNNGCACAAAGGGCTCCATG | NGG | 2 | 0.4179 | Tier III |
30 | NC_000020.11 | 20 | 58579960 | - | APCDD1L-DT | NNNCCTCAGAAGGCTCCATG | NGG | 2 | 0.3782 | Tier III |
31 | NC_000023.11 | X | 129015401 | + | LOC124905213 | NNNGTACAGAAGGCTCCTTG | NGG | 2 | 0.3182 | Tier III |
32 | NC_000001.11 | 1 | 8140088 | + | LOC107984915 | NNNGCACAAAAGGCTCTATG | NGG | 2 | 0.3 | Tier III |
33 | NC_000009.12 | 9 | 86458021 | + | LOC102724080 | NNNGCACAAAAGGCTCTATG | NGG | 2 | 0.3 | Tier III |
34 | NC_000002.12 | 2 | 22541355 | + | LINC01830 | NNNGCACAGAAGACTCCATG | NAG | 2 | 0.2393 | Tier III |
35 | NC_000022.11 | 22 | 29410938 | - | LOC102723305 | NNNGAACAGCAGGCTCCATG | NGG | 2 | 0.1905 | Tier III |
36 | NC_000010.11 | 10 | 24756429 | - | LOC105376456 | NNNGCACATAAGGCTTCATG | NGG | 2 | 0.1641 | Tier III |
37 | NC_000008.11 | 8 | 129370627 | + | CCDC26 | NNNTCACAGAAGGCTCCATC | NGG | 2 | 0.1558 | Tier III |
38 | NC_000006.12 | 6 | 5063265 | - | LYRM4-AS1 | NNNGCACAGAATGCTTCATG | NGG | 2 | 0.1183 | Tier III |
39 | NC_000017.11 | 17 | 74104663 | + | LINC02074 | NNNGCACAGAAGGCCCCATG | NTG | 2 | 0.0106 | Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
Reference Sequence | Chromosome | Cut Position | Match Strand | Match Gene | Match Sequence | PAM Seq. | Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. | CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. | Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. | |
---|---|---|---|---|---|---|---|---|---|---|
1 | NC_000067.6 | 1 | 80213066 | + | Fam124b | NNNGCACAGAAGACTCCTTG | NGG | 2 | 0.4615 | Tier I |
2 | NC_000086.7 | X | 164108415 | - | Cltrn | NNNGCACAGATGGCTCCATC | NGG | 2 | 0.1319 | Tier I |
3 | NC_000073.6 | 7 | 127934615 | + | Prss36 | NNNGCACAGAAGGCTCCAAC | NGG | 2 | 0.1224 | Tier I |
4 | NC_000074.6 | 8 | 32412577 | - | Nrg1 | NNNGCAAAGAAGACTCCATG | NGG | 2 | 0.6923 | Tier II |
5 | NC_000074.6 | 8 | 124250881 | - | Galnt2 | NNNGCACAGGAGGCTCCATG | NGG | 1 | 0.5556 | Tier II |
6 | NC_000072.6 | 6 | 6065630 | - | Slc25a13 | NNNAAACAGAAGGCTCCATG | NGG | 2 | 0.5143 | Tier II |
7 | NC_000077.6 | 11 | 118625134 | - | Rbfox3 | NNNAAACAGAAGGCTCCATG | NGG | 2 | 0.5143 | Tier II |
8 | NC_000067.6 | 1 | 156920688 | + | Ralgps2 | NNNGCACAGAAGGCTAAATG | NGG | 2 | 0.4667 | Tier II |
9 | NC_000077.6 | 11 | 67052931 | - | Sco1 | NNNGCCCAGAAGGCTCCATG | NGG | 1 | 0.4545 | Tier II |
10 | NC_000067.6 | 1 | 105801709 | - | Tnfrsf11a | NNNCCACGGAAGGCTCCATG | NGG | 2 | 0.3882 | Tier II |
11 | NC_000076.6 | 10 | 123670781 | + | Tafa2 | NNNACACAGAAGGCTCCATC | NGG | 2 | 0.3857 | Tier II |
12 | NC_000072.6 | 6 | 52788450 | + | Jazf1 | NNNGGACAAAAGGCTCCATG | NGG | 2 | 0.3857 | Tier II |
13 | NC_000072.6 | 6 | 115888357 | + | Ift122 | NNNGCACAGAGGCCTCCATG | NGG | 2 | 0.2737 | Tier II |
14 | NC_000074.6 | 8 | 88562605 | - | Nkd1 | NNNGCACACAAGGCTCCTTG | NGG | 2 | 0.2692 | Tier II |
15 | NC_000083.6 | 17 | 67312074 | + | Ptprm | NNNGCACAGGAGGATCCATG | NGG | 2 | 0.1944 | Tier II |
16 | NC_000068.7 | 2 | 91987159 | + | Creb3l1 | NNNGCACAGAAGTCACCATG | NGG | 2 | 0.1737 | Tier II |
17 | NC_000067.6 | 1 | 152414534 | - | Colgalt2 | NNNGCACAGAACGCTCCAAG | NGG | 2 | 0.1513 | Tier II |
18 | NC_000079.6 | 13 | 108325185 | - | Depdc1b | NNNGCACAGGAGGCTCCAGG | NGG | 2 | 0.1389 | Tier II |
19 | NC_000077.6 | 11 | 119868767 | - | Rptor | NNNCCACAGAAGGCTCCATG | NAG | 2 | 0.1373 | Tier II |
20 | NC_000085.6 | 19 | 5723986 | + | Ehbp1l1 | NNNGCAGAGAAGGCTCCAGG | NGG | 2 | 0.1176 | Tier II |
21 | NC_000067.6 | 1 | 194696373 | - | Plxna2 | NNNGCCCAGAAGGCTCCAGG | NGG | 2 | 0.1136 | Tier II |
22 | NC_000074.6 | 8 | 70652565 | - | Pgpep1 | NNNGCACAGAAGGCTCCGTG | NAG | 2 | 0.1037 | Tier II |
23 | NC_000076.6 | 10 | 85421428 | + | Btbd11 | NNNGCACAGAAGGCAGCATG | NGG | 2 | 0.0891 | Tier II |
24 | NC_000078.6 | 12 | 53773940 | - | Npas3 | NNNGCACAGAAGTCTCCATG | NAG | 2 | 0.0778 | Tier II |
25 | NC_000075.6 | 9 | 58049236 | - | Ccdc33 | NNNGCACAGAAGGCCCCATG | NAG | 2 | 0.0707 | Tier II |
26 | NC_000073.6 | 7 | 4491974 | - | Ppp1r12c | NNNGCACAGAAGGATCCCTG | NGG | 2 | 0.0667 | Tier II |
27 | NC_000073.6 | 7 | 82534454 | - | Adamtsl3 | NNNGCACAGAAGGCTCCAGG | NAG | 2 | 0.0648 | Tier II |
28 | NC_000074.6 | 8 | 14613638 | - | Dlgap2 | NNNGCACACAAGGCTCCATG | NTG | 2 | 0.021 | Tier II |
29 | NC_000074.6 | 8 | 122797838 | + | Acsf3 | NNNGCACTGAAGGCTCCATG | NTG | 2 | 0.0167 | Tier II |
30 | NC_000074.6 | 8 | 40356088 | + | Micu3 | NNNGCACAGAAGCCTCCATG | NTG | 2 | 0.0164 | Tier II |
31 | NC_000082.6 | 16 | 70337933 | - | Gbe1 | NNNGCACAGAAGGCTCCATT | NGC | 2 | 0.0156 | Tier II |
32 | NC_000069.6 | 3 | 9516624 | - | Zfp704 | NNNGCACAGAAGCCTCCATG | NGC | 2 | 0.0094 | Tier II |
33 | NC_000067.6 | 1 | 80213066 | + | 2310015K22Rik | NNNGCACAGAAGACTCCTTG | NGG | 2 | 0.4615 | Tier III |
34 | NC_000067.6 | 1 | 80213066 | + | LOC108167342 | NNNGCACAGAAGACTCCTTG | NGG | 2 | 0.4615 | Tier III |
35 | NC_000072.6 | 6 | 52788450 | + | Gm52904 | NNNGGACAAAAGGCTCCATG | NGG | 2 | 0.3857 | Tier III |
36 | NC_000077.6 | 11 | 112891678 | - | Gm46301 | NNNGAACAGAAGGCTTCATG | NGG | 2 | 0.1758 | Tier III |
37 | NC_000067.6 | 1 | 194696373 | - | Gm46202 | NNNGCCCAGAAGGCTCCAGG | NGG | 2 | 0.1136 | Tier III |
38 | NC_000081.6 | 15 | 79022182 | - | Gm17753 | NNNGCACAGAAGGCTCCCTG | NAG | 2 | 0.0494 | Tier III |
39 | NC_000067.6 | 1 | 105423141 | + | Gm28403 | NNNGCACAGAATGCTCCATG | NTG | 2 | 0.015 | Tier III |