Construct: sgRNA BRDN0001146101
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AGCAACGAGCAGTACAATGT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- TNIK (23043)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75850
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000003.12 |
3 |
171093933 |
- |
TNIK |
NNNAACGAGCAGTACAATGT |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000008.11 |
8 |
48440654 |
- |
LOC105375821 |
NNNTACTAGCAGTACAATGT |
NGG |
2 |
0.3636 |
Tier III |
| 3 |
NC_000011.10 |
11 |
71851052 |
- |
XNDC1N-ZNF705EP-ALG1L9P |
NNNAACGAGGAGTACAACGT |
NGG |
2 |
0.1667 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000081.6 |
15 |
102716231 |
- |
Calcoco1 |
NNNACCGAGCAGTACAAGGT |
NGG |
2 |
0.1667 |
Tier I |
| 2 |
NC_000069.6 |
3 |
139703902 |
+ |
Stpg2 |
NNNAGAGAGCAGTACAATGT |
NGG |
2 |
0.6686 |
Tier II |
| 3 |
NC_000070.6 |
4 |
76096623 |
+ |
Ptprd |
NNNAAAGAGCAGTACAATAT |
NGG |
2 |
0.6633 |
Tier II |
| 4 |
NC_000078.6 |
12 |
3958058 |
- |
Pomc |
NNNAAGGAGCAGAACAATGT |
NGG |
2 |
0.3462 |
Tier II |
| 5 |
NC_000086.7 |
X |
42830875 |
+ |
Tenm1 |
NNNATCGAGCAGTACAATTT |
NGG |
2 |
0.2424 |
Tier II |
| 6 |
NC_000071.6 |
5 |
30159958 |
+ |
Hadhb |
NNNAAAGAGCAGTACAGTGT |
NGG |
2 |
0.1639 |
Tier II |
| 7 |
NC_000086.7 |
X |
43121872 |
+ |
Tenm1 |
NNNAAGGAGCTGTACAATGT |
NGG |
2 |
0.1538 |
Tier II |
| 8 |
NC_000081.6 |
15 |
37000006 |
- |
Zfp706 |
NNNAACGAGCAGAACATTGT |
NGG |
2 |
0.0923 |
Tier II |
| 9 |
NC_000067.6 |
1 |
43173973 |
- |
Fhl2 |
NNNAACTAGCAGTACAATGT |
NTG |
2 |
0.0223 |
Tier II |
| 10 |
NC_000070.6 |
4 |
135247587 |
- |
Clic4 |
NNNAACGAGCAGTACAGTGT |
NTG |
2 |
0.0069 |
Tier II |
Other clones with same target sequence:
(none)
