Construct: sgRNA BRDN0001146121
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- ATGTACGGGAAGGACAAAGT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- IPMK (253430)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 78075
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000010.11 |
10 |
58267427 |
- |
IPMK |
NNNTACGGGAAGGACAAAGT |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000016.10 |
16 |
71640456 |
- |
MARVELD3 |
NNNTCCGGCAAGGACAAAGT |
NGG |
2 |
0.2692 |
Tier I |
| 3 |
NC_000006.12 |
6 |
20429625 |
+ |
E2F3 |
NNNTAAGAGAAGGACAAAGT |
NGG |
2 |
0.9286 |
Tier II |
| 4 |
NC_000007.14 |
7 |
114623690 |
+ |
FOXP2 |
NNNTAAGAGAAGGACAAAGT |
NGG |
2 |
0.9286 |
Tier II |
| 5 |
NC_000016.10 |
16 |
19031068 |
+ |
TMC7 |
NNNTACAGGAAGGACAAAGA |
NGG |
2 |
0.5625 |
Tier II |
| 6 |
NC_000006.12 |
6 |
25551492 |
- |
CARMIL1 |
NNNTATGGGAAGGTCAAAGT |
NGG |
2 |
0.4952 |
Tier II |
| 7 |
NC_000010.11 |
10 |
106732195 |
- |
SORCS1 |
NNNTAGGGGAAGAACAAAGT |
NGG |
2 |
0.4615 |
Tier II |
| 8 |
NC_000010.11 |
10 |
51431400 |
+ |
PRKG1 |
NNNCACGGGAAGGACAAGGT |
NGG |
2 |
0.2588 |
Tier II |
| 9 |
NC_000010.11 |
10 |
96241689 |
- |
BLNK |
NNNTAGGGGAAGCACAAAGT |
NGG |
2 |
0.2105 |
Tier II |
| 10 |
NC_000006.12 |
6 |
77431634 |
+ |
LOC105377864 |
NNNTAGGGGAAGGACAGAGT |
NGG |
2 |
0.0882 |
Tier II |
| 11 |
NC_000010.11 |
10 |
113551485 |
+ |
HABP2 |
NNNTAGGGGAAGGACAGAGT |
NGG |
2 |
0.0882 |
Tier II |
| 12 |
NC_000016.10 |
16 |
10987831 |
- |
CLEC16A |
NNNTACGGGAAGGAGAAAGT |
NGG |
1 |
0.05 |
Tier II |
| 13 |
NC_000001.11 |
1 |
62060189 |
- |
PATJ |
NNNTACGGGAAGGACAGAGG |
NGG |
2 |
0.0311 |
Tier II |
| 14 |
NC_000019.10 |
19 |
15911640 |
- |
LOC729654 |
NNNTATGGGAAGGACAAAAT |
NGG |
2 |
0.6633 |
Tier III |
| 15 |
NC_000020.11 |
20 |
37577571 |
+ |
LINC01746 |
NNNTAGGGGAAGGACAAAGT |
NGG |
1 |
0.5 |
Tier III |
| 16 |
NC_000013.11 |
13 |
22838059 |
- |
IPMKP1 |
NNNAACGGGAAGGACAAAGT |
NGG |
1 |
0.4762 |
Tier III |
| 17 |
NC_000016.10 |
16 |
71640456 |
- |
LOC124903711 |
NNNTCCGGCAAGGACAAAGT |
NGG |
2 |
0.2692 |
Tier III |
| 18 |
NC_000001.11 |
1 |
221830846 |
- |
LINC01655 |
NNNTATGGGAAGGACAGAGT |
NGG |
2 |
0.1639 |
Tier III |
| 19 |
NC_000007.14 |
7 |
10468586 |
- |
MGC4859 |
NNNTACGGGCAGGACAAAGG |
NGG |
2 |
0.0588 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000076.6 |
10 |
71348086 |
+ |
Ipmk |
NNNTACGGCAAGGACAAAGT |
NGG |
1 |
0.5385 |
Tier I |
| 2 |
NC_000071.6 |
5 |
138758435 |
+ |
Fam20c |
NNNGAAGGGAAGGACAAAGT |
NGG |
2 |
0.5804 |
Tier II |
| 3 |
NC_000068.7 |
2 |
52972186 |
+ |
Fmnl2 |
NNNTCTGGGAAGGACAAAGT |
NGG |
2 |
0.4643 |
Tier II |
| 4 |
NC_000082.6 |
16 |
10585561 |
- |
Clec16a |
NNNTACAGGAAGGAGAAAGT |
NGG |
2 |
0.05 |
Tier II |
| 5 |
NC_000081.6 |
15 |
36923485 |
+ |
Gm41300 |
NNNTACGGGAAGAACAAAAT |
NGG |
2 |
0.6593 |
Tier III |
Other clones with same target sequence:
(none)
