Construct: sgRNA BRDN0001146152

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
GTTATTGAATATCTCATGGG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TRPM7 (54822)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76111

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000015.10 15 50614199 - TRPM7 NNNATTGAATATCTCATGGG NGG 0 1.0 Tier I
2 NC_000004.12 4 52027993 - SGCB NNNATTGAATTTCACATGGG NGG 2 0.1905 Tier I
3 NC_000002.12 2 164508083 + GRB14 NNNATTAAATATCTCAAGGG NGG 2 0.5333 Tier II
4 NC_000009.12 9 5300914 + RLN2 NNNATTGAATATTTCATGGT NGG 2 0.49 Tier II
5 NC_000005.10 5 90881548 + ADGRV1 NNNATTGAATAACTCAAGGG NGG 2 0.4267 Tier II
6 NC_000007.14 7 14364729 - DGKB NNNATTAAATCTCTCATGGG NGG 2 0.4 Tier II
7 NC_000021.9 21 40326794 + DSCAM NNNGTTGAATATCACATGGG NGG 2 0.3869 Tier II
8 NC_000014.9 14 92982661 - ITPK1 NNNATTTATTATCTCATGGG NGG 2 0.3429 Tier II
9 NC_000014.9 14 78631094 - NRXN3 NNNAATGATTATCTCATGGG NGG 2 0.3 Tier II
10 NC_000021.9 21 33237727 + IFNAR2 NNNCTTGGATATCTCATGGG NGG 2 0.2588 Tier II
11 NC_000021.9 21 33237727 + IFNAR2-IL10RB NNNCTTGGATATCTCATGGG NGG 2 0.2588 Tier II
12 NC_000010.11 10 51209092 - PRKG1 NNNAGTGAATATATCATGGG NGG 2 0.2462 Tier II
13 NC_000002.12 2 50656274 - NRXN1 NNNATTTAATTTCTCATGGG NGG 2 0.1758 Tier II
14 NC_000018.10 18 7741875 + PTPRM NNNATTGAAGATCCCATGGG NGG 2 0.1429 Tier II
15 NC_000009.12 9 6861259 - KDM4C NNNGTTGAATATCTCATGGG NGA 2 0.0434 Tier II
16 NC_000012.12 12 102903558 + PAH NNNATTGAATATCTGATGAG NGG 2 0.0357 Tier II
17 NC_000023.11 X 70428269 - GDPD2 NNNATTGAATATCTCATGTG NTG 2 0.026 Tier II
18 NC_000004.12 4 92690739 - GRID2 NNNATTGAATTTCTCATGGG NTG 2 0.012 Tier II
19 NC_000020.11 20 21674774 + LINC01726 NNNATTGAATATTTCATGGA NGG 2 0.6563 Tier III
20 NC_000002.12 2 51720684 - NRXN1-DT NNNATTCAATATCTCATGGA NGG 2 0.6445 Tier III
21 NC_000018.10 18 39346415 - MIR924HG NNNATTCAATATCACATGGG NGG 2 0.4256 Tier III
22 NC_000007.14 7 17268177 + LOC101927609 NNNATTGCATATATCATGGG NGG 2 0.1648 Tier III
23 NC_000018.10 18 7741875 + LOC124904242 NNNATTGAAGATCCCATGGG NGG 2 0.1429 Tier III
24 NC_000022.11 22 26662878 + MIAT NNNAATGAATATCTCATGGG NAG 2 0.1296 Tier III
25 NC_000008.11 8 60361142 - LOC105375864 NNNATTGTATATCCCATGGG NGG 2 0.1224 Tier III
26 NC_000011.10 11 112490395 + LINC02763 NNNATTCAATATGTCATGGG NGG 2 0.0938 Tier III
27 NC_000012.12 12 102903558 + LOC124902999 NNNATTGAATATCTGATGAG NGG 2 0.0357 Tier III
28 NC_000023.11 X 70428269 - LOC105373244 NNNATTGAATATCTCATGTG NTG 2 0.026 Tier III
29 NC_000023.11 X 113145654 - LOC101928437 NNNATTGAATATATCATGGG NTG 2 0.015 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000068.7 2 126831273 - Trpm7 NNNATTGAGTATCTCATGGG NGG 1 0.6667 Tier I
2 NC_000071.6 5 73639408 - Sgcb NNNATTGAATTTCACATGGG NGG 2 0.1905 Tier I
3 NC_000075.6 9 60678402 + Lrrc49 NNNAGTGAAAATCTCATGGG NGG 2 0.5486 Tier II
4 NC_000076.6 10 71337187 - Cisd1 NNNATTGAATATTTCATGTG NGG 2 0.4667 Tier II
5 NC_000078.6 12 38414130 + Dgkb NNNATTAAATCTCTCATGGG NGG 2 0.4 Tier II
6 NC_000075.6 9 45856360 + Bace1 NNNATTGAATCTCTCATGGA NGG 2 0.375 Tier II
7 NC_000067.6 1 163255320 + Prrx1 NNNAATGAATATCTCATGTG NGG 2 0.3333 Tier II
8 NC_000071.6 5 53065088 + Slc34a2 NNNCTTGAATATCTCATGGA NGG 2 0.3309 Tier II
9 NC_000079.6 13 29383718 - Cdkal1 NNNATGGAATCTCTCATGGG NGG 2 0.2286 Tier II
10 NC_000071.6 5 9514986 + Grm3 NNNATTGAATATCTAATGAG NGG 2 0.1587 Tier II
11 NC_000079.6 13 67545847 - Zfp748 NNNATTGAACATCTCATGGG NGC 2 0.0119 Tier II
12 NC_000084.6 18 74549853 - Myo5b NNNATTGAATATCTCATGTG NGT 2 0.0108 Tier II
13 NC_000068.7 2 139605550 - Sptlc3 NNNATTGAATATGTCTTGGG NGG 2 0.0 Tier II
14 NC_000070.6 4 3725429 - Lyn NNNATTGAATGTCTCTTGGG NGG 2 0.0 Tier II
15 NC_000074.6 8 118427080 + Cdh13 NNNATTGACTATCTCCTGGG NGG 2 0.0 Tier II
16 NC_000080.6 14 99538148 - Gm41230 NNNATTGAAGATTTCATGGG NGG 2 0.35 Tier III
17 NC_000072.6 6 68806626 + Igk NNNATTTTATATCTCATGGG NGG 2 0.2449 Tier III
18 NC_000072.6 6 68907993 + Igk NNNATTTTATATCTCATGGG NGG 2 0.2449 Tier III
19 NC_000072.6 6 69057923 + Igk NNNATTTTATATCTCATGGG NGG 2 0.2449 Tier III
20 NC_000072.6 6 69585496 + Igk NNNATTTTATATCTCATGGG NGG 2 0.2449 Tier III
21 NC_000086.7 X 155655903 + Gm15155 NNNGTTGAATATGTCATGGG NGG 2 0.0852 Tier III
22 NC_000067.6 1 48881870 - Gm8357 NNNATTGAATTTGTCATGGG NGG 2 0.042 Tier III
23 NC_000071.6 5 98153336 - Gm8048 NNNATTGAATTTGTCATGGG NGG 2 0.042 Tier III
24 NC_000077.6 11 58477886 + Olfr333-ps1 NNNATTGAATATCTCAAGGG NTG 2 0.0208 Tier III
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Other clones with same target sequence:

(none)