Construct: sgRNA BRDN0001146165
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TTCACAAAATTAGAGCGCAT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- STK26 (51765)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75999
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000023.11 |
X |
132054675 |
+ |
STK26 |
NNNACAAAATTAGAGCGCAT |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000003.12 |
3 |
25089343 |
+ |
RARB |
NNNACAAAATTAGAGTACAT |
NGG |
2 |
0.2872 |
Tier II |
| 3 |
NC_000001.11 |
1 |
217126750 |
+ |
ESRRG |
NNNAAAAAATTAGAGTGCAT |
NGG |
2 |
0.1758 |
Tier II |
| 4 |
NC_000002.12 |
2 |
201728811 |
+ |
ALS2 |
NNNACAAAATTAGAGCTCAA |
NGG |
2 |
0.1406 |
Tier II |
| 5 |
NC_000007.14 |
7 |
26746068 |
- |
SKAP2 |
NNNACAAAATTACAGTGCAT |
NGG |
2 |
0.1296 |
Tier II |
| 6 |
NC_000001.11 |
1 |
241314671 |
+ |
RGS7 |
NNNACAAAAGTAGAGCGCAG |
NGG |
2 |
0.0882 |
Tier II |
| 7 |
NC_000015.10 |
15 |
65883713 |
- |
RAB11A |
NNNACAAAATTAGAGAGCAT |
NGT |
2 |
0.0161 |
Tier II |
| 8 |
NC_000011.10 |
11 |
41431895 |
- |
LRRC4C |
NNNACAAAATCAGAGCGCAT |
NGC |
2 |
0.0148 |
Tier II |
| 9 |
NC_000002.12 |
2 |
24700003 |
+ |
NCOA1 |
NNNACAAAATTAGCGCGCAT |
NGT |
2 |
0.0035 |
Tier II |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000086.7 |
X |
50870300 |
+ |
Stk26 |
NNNACAAAATTAGAGCGCAT |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000071.6 |
5 |
102250658 |
- |
Arhgap24 |
NNNACAAAATTAGTGTGCAT |
NGG |
2 |
0.1641 |
Tier II |
| 3 |
NC_000067.6 |
1 |
176998567 |
+ |
Sdccag8 |
NNNACAAAATTAGAGCGCAA |
NGC |
2 |
0.0125 |
Tier II |
| 4 |
NC_000077.6 |
11 |
52827038 |
- |
Fstl4 |
NNNACAAAATGAGAGCGCAT |
NGC |
2 |
0.0089 |
Tier II |
Other clones with same target sequence:
(none)
