Construct: sgRNA BRDN0001146195
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GAGCCATCGGACTACAGCAA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- ALPK1 (80216)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76082
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000004.12 |
4 |
112431236 |
+ |
ALPK1 |
NNNCCATCGGACTACAGCAA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000006.12 |
6 |
162406264 |
- |
PRKN |
NNNCCATCTGACTTCAGCAA |
NGG |
2 |
0.2844 |
Tier II |
| 3 |
NC_000017.11 |
17 |
56171448 |
+ |
ANKFN1 |
NNNCCAACGGTCTACAGCAA |
NGG |
2 |
0.2692 |
Tier II |
| 4 |
NC_000001.11 |
1 |
26038984 |
- |
SLC30A2 |
NNNCCATCTGACTACAGCCA |
NGG |
2 |
0.1103 |
Tier II |
| 5 |
NC_000006.12 |
6 |
39447800 |
- |
KIF6 |
NNNCCATTGGACTACAGCAA |
NGC |
2 |
0.0194 |
Tier II |
| 6 |
NC_000023.11 |
X |
119792758 |
+ |
LOC107985694 |
NNNCCAACGGATTACAGCAA |
NGG |
2 |
0.4712 |
Tier III |
| 7 |
NC_000006.12 |
6 |
162406264 |
- |
LOC105369171 |
NNNCCATCTGACTTCAGCAA |
NGG |
2 |
0.2844 |
Tier III |
| 8 |
NC_000002.12 |
2 |
89144574 |
- |
IGK |
NNNCCATCTGACTACAGCAA |
NTG |
2 |
0.0208 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000070.6 |
4 |
93334809 |
+ |
Tusc1 |
NNNCCCTCGGACTACAGCCA |
NGG |
2 |
0.094 |
Tier I |
| 2 |
NC_000080.6 |
14 |
115755415 |
- |
Gpc5 |
NNNACATAGGACTACAGCAA |
NGG |
2 |
0.5474 |
Tier II |
| 3 |
NC_000086.7 |
X |
9470134 |
+ |
Cybb |
NNNACATAGGACTACAGCAA |
NGG |
2 |
0.5474 |
Tier II |
| 4 |
NC_000085.6 |
19 |
28483910 |
+ |
Glis3 |
NNNCCATCAGACCACAGCAA |
NGG |
2 |
0.5075 |
Tier II |
| 5 |
NC_000078.6 |
12 |
100478097 |
+ |
Ttc7b |
NNNCCATCCAACTACAGCAA |
NGG |
2 |
0.5026 |
Tier II |
| 6 |
NC_000078.6 |
12 |
100478097 |
+ |
Gm36375 |
NNNCCATCCAACTACAGCAA |
NGG |
2 |
0.5026 |
Tier II |
| 7 |
NC_000083.6 |
17 |
27858617 |
- |
Uhrf1bp1 |
NNNGCATCGGACTACAGCAA |
NGG |
1 |
0.5 |
Tier II |
| 8 |
NC_000080.6 |
14 |
103661472 |
- |
Slain1 |
NNNGCATCGGACTACAGCAG |
NGG |
2 |
0.3824 |
Tier II |
| 9 |
NC_000076.6 |
10 |
40070407 |
- |
Slc16a10 |
NNNCCATTGGCCTACAGCAA |
NGG |
2 |
0.35 |
Tier II |
| 10 |
NC_000084.6 |
18 |
67560612 |
+ |
Spire1 |
NNNCCATGGGACTTCAGCAA |
NGG |
2 |
0.3429 |
Tier II |
| 11 |
NC_000073.6 |
7 |
144030983 |
- |
Shank2 |
NNNCCAGCTGACTACAGCAA |
NGG |
2 |
0.3137 |
Tier II |
| 12 |
NC_000076.6 |
10 |
98565219 |
+ |
Gm34297 |
NNNCCAAAGGACTACAGCAA |
NGG |
2 |
0.5688 |
Tier III |
| 13 |
NC_000081.6 |
15 |
62133378 |
- |
Pvt1 |
NNNTTATCGGACTACAGCAA |
NGG |
2 |
0.5091 |
Tier III |
| 14 |
NC_000084.6 |
18 |
67560612 |
+ |
Gm17669 |
NNNCCATGGGACTTCAGCAA |
NGG |
2 |
0.3429 |
Tier III |
Other clones with same target sequence:
(none)
