Construct: sgRNA BRDN0001146197
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AGCGGCGACACGACAGACAT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- ERBB4 (2066)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76197
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000002.12 |
2 |
211679115 |
+ |
ERBB4 |
NNNGGCGACACGACAGACAT |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000001.11 |
1 |
5881528 |
+ |
NPHP4 |
NNNGGCGACACCAAAGACAT |
NGG |
2 |
0.1853 |
Tier II |
| 3 |
NC_000010.11 |
10 |
132313403 |
+ |
STK32C |
NNNGGCCACACGACAGACAT |
NTG |
2 |
0.0268 |
Tier II |
| 4 |
NC_000023.11 |
X |
69068413 |
+ |
LOC105373242 |
NNNGGAGACACGACAGACAT |
NGA |
2 |
0.0645 |
Tier III |
| 5 |
NC_000002.12 |
2 |
130744683 |
- |
LOC124907890 |
NNNGGCGACAGGACAGCCAT |
NGG |
2 |
0.0441 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000071.6 |
5 |
122729856 |
- |
Camkk2 |
NNNGGTGACACTACAGACAT |
NGG |
2 |
0.3571 |
Tier II |
| 2 |
NC_000069.6 |
3 |
9828570 |
+ |
Pag1 |
NNNGGCTACACCACAGACAT |
NGG |
2 |
0.3025 |
Tier II |
| 3 |
NC_000075.6 |
9 |
121373016 |
- |
Trak1 |
NNNGGCGACACCACAGTCAT |
NGG |
2 |
0.0706 |
Tier II |
Other clones with same target sequence:
(none)
