Construct: sgRNA BRDN0001146221
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GAGTGCACTATTAAACTGTG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- TEX14 (56155)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76832
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000017.11 |
17 |
58587978 |
+ |
TEX14 |
NNNTGCACTATTAAACTGTG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000001.11 |
1 |
28745418 |
- |
YTHDF2 |
NNNTAAACTATTAAACTGTG |
NGG |
2 |
0.8048 |
Tier II |
| 3 |
NC_000008.11 |
8 |
70341531 |
- |
NCOA2 |
NNNTGGACAATTAAACTGTG |
NGG |
2 |
0.4643 |
Tier II |
| 4 |
NC_000012.12 |
12 |
119715954 |
+ |
CIT |
NNNTGCACTTTGAAACTGTG |
NGG |
2 |
0.4412 |
Tier II |
| 5 |
NC_000001.11 |
1 |
239730361 |
- |
CHRM3 |
NNNTCCTCTATTAAACTGTG |
NGG |
2 |
0.3437 |
Tier II |
| 6 |
NC_000014.9 |
14 |
50279377 |
+ |
L2HGDH |
NNNTGAACTCTTAAACTGTG |
NGG |
2 |
0.3095 |
Tier II |
| 7 |
NC_000007.14 |
7 |
117866875 |
- |
CTTNBP2 |
NNNTGCACTATTAAACTGGG |
NGG |
1 |
0.25 |
Tier II |
| 8 |
NC_000016.10 |
16 |
74938842 |
- |
WDR59 |
NNNTGCACTGTTAAAGTGTG |
NGG |
2 |
0.0855 |
Tier II |
| 9 |
NC_000007.14 |
7 |
18504741 |
- |
HDAC9 |
NNNTGCATTATTAAACTGTG |
NTG |
2 |
0.0341 |
Tier II |
| 10 |
NC_000005.10 |
5 |
77753344 |
- |
TBCA |
NNNTTCACTATTAAACTGTG |
NGA |
2 |
0.0208 |
Tier II |
| 11 |
NC_000003.12 |
3 |
105520276 |
+ |
ALCAM |
NNNTGCAATATTAAACTGTG |
NGT |
2 |
0.0105 |
Tier II |
| 12 |
NC_000006.12 |
6 |
94568200 |
+ |
LOC105377901 |
NNNTGCACTCATAAACTGTG |
NGG |
2 |
0.25 |
Tier III |
| 13 |
NC_000019.10 |
19 |
35084674 |
+ |
HPN-AS1 |
NNNTGCAGTATTAAACTGGG |
NGG |
2 |
0.1607 |
Tier III |
| 14 |
NC_000019.10 |
19 |
35084674 |
+ |
LOC100420797 |
NNNTGCAGTATTAAACTGGG |
NGG |
2 |
0.1607 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000083.6 |
17 |
46891328 |
+ |
Tbcc |
NNNTGCACTGTCAAACTGTG |
NGG |
2 |
0.5263 |
Tier I |
| 2 |
NC_000079.6 |
13 |
42974650 |
- |
Phactr1 |
NNNTGCACTATCAAACTGTA |
NGG |
2 |
0.8882 |
Tier II |
| 3 |
NC_000068.7 |
2 |
55465092 |
+ |
Kcnj3 |
NNNTGCATTATTAAACTGTG |
NGG |
1 |
0.875 |
Tier II |
| 4 |
NC_000078.6 |
12 |
53961689 |
+ |
Npas3 |
NNNTGCATAATTAAACTGTG |
NGG |
2 |
0.8125 |
Tier II |
| 5 |
NC_000070.6 |
4 |
45925905 |
- |
Ccdc180 |
NNNGGCACTATTAAAATGTG |
NGG |
2 |
0.625 |
Tier II |
| 6 |
NC_000078.6 |
12 |
19535478 |
+ |
Gm6943 |
NNNGGCACTATTAAAATGTG |
NGG |
2 |
0.625 |
Tier II |
| 7 |
NC_000068.7 |
2 |
62939285 |
- |
Kcnh7 |
NNNGGCACTATTAAACTGTG |
NGG |
1 |
0.625 |
Tier II |
| 8 |
NC_000078.6 |
12 |
100150547 |
+ |
Nrde2 |
NNNTGCTCTATCAAACTGTG |
NGG |
2 |
0.4145 |
Tier II |
| 9 |
NC_000067.6 |
1 |
33920326 |
+ |
Dst |
NNNTGCACTATGGAACTGTG |
NGG |
2 |
0.3261 |
Tier II |
| 10 |
NC_000076.6 |
10 |
110752264 |
- |
E2f7 |
NNNGGCACTATTAGACTGTG |
NGG |
2 |
0.2917 |
Tier II |
| 11 |
NC_000069.6 |
3 |
103338355 |
- |
Trim33 |
NNNTGGACTATTAACCTGTG |
NGG |
2 |
0.1364 |
Tier II |
| 12 |
NC_000067.6 |
1 |
127336999 |
- |
Mgat5 |
NNNTGCACTCTTAAACTGTG |
NGC |
2 |
0.0074 |
Tier II |
| 13 |
NC_000079.6 |
13 |
80992504 |
+ |
9330111N05Rik |
NNNTGCACTTTTAAACTGTG |
NGC |
2 |
0.0196 |
Tier III |
Other clones with same target sequence:
(none)
