Construct: sgRNA BRDN0001146342

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
ATTATGAACAGTGTGCATCG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
EIF2AK2 (5610)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
75637

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000002.12 2 37141576 - EIF2AK2 NNNATGAACAGTGTGCATCG NGG 0 1.0 Tier I
2 NC_000012.12 12 49670186 + FMNL3 NNNATGAACTGTGTGCATCC NGG 2 0.3782 Tier I
3 NC_000021.9 21 17550044 - CXADR NNNATGAACTGTGTGCATCT NGG 2 0.6176 Tier II
4 NC_000008.11 8 39994979 - IDO2 NNNATGAACAGTGTGAATAG NGG 2 0.4286 Tier II
5 NC_000009.12 9 37630759 + FRMPD1 NNNATGGACAGTGTGCATCC NGG 2 0.3025 Tier II
6 NC_000001.11 1 216989936 + ESRRG NNNATGAACAGTGTGCATGG NGG 1 0.125 Tier II
7 NC_000010.11 10 55372530 + PCDH15 NNNATGAACAGTGTGCATGA NGG 2 0.1172 Tier II
8 NC_000017.11 17 83037689 - QTGAL NNNTTGAACAGTGTGCCTCG NGG 2 0.1123 Tier II
9 NC_000002.12 2 23492299 - KLHL29 NNNATGAACAGTGTGCAAGG NGG 2 0.0833 Tier II
10 NC_000002.12 2 96507236 - NEURL3 NNNATGAAGAGTGGGCATCG NGG 2 0.0 Tier II
11 NC_000002.12 2 23030835 + LOC107985792 NNNATGAACACTGTGCATCC NGG 2 0.1837 Tier III
Download CSV

Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000072.6 6 76959771 + Ctnna2 NNNATGAACAGTATGAATCG NGG 2 0.9231 Tier II
2 NC_000077.6 11 109580350 + Wipi1 NNNATGAACAGTGAGCATCG NGG 1 0.619 Tier II
3 NC_000081.6 15 21127961 - Cdh12 NNNATGAACAGTGAGCATCT NGG 2 0.4333 Tier II
4 NC_000073.6 7 30294457 - Clip3 NNNATGGACAGTGTGCACCG NGG 2 0.3025 Tier II
5 NC_000067.6 1 127911772 + Rab3gap1 NNNATGAACAGTGTGTATAG NGG 2 0.1319 Tier II
6 NC_000068.7 2 33848952 + Mvb12b NNNATGAACAGTGAGCCTCG NGG 2 0.1092 Tier II
7 NC_000067.6 1 43151679 - Fhl2 NNNATGAACAGTGTGGATCT NGG 2 0.1077 Tier II
8 NC_000080.6 14 64866590 - Hmbox1 NNNATGAACATTGTGCATGG NGG 2 0.0481 Tier II
9 NC_000077.6 11 109580350 + Gm39442 NNNATGAACAGTGAGCATCG NGG 1 0.619 Tier III
10 NC_000071.6 5 64482224 - C030018K13Rik NNNAGGAACAGTGTGCATGG NGG 2 0.08 Tier III
Download CSV

Other clones with same target sequence:

(none)

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