Construct: sgRNA BRDN0001146342
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- ATTATGAACAGTGTGCATCG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- EIF2AK2 (5610)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75637
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000002.12 |
2 |
37141576 |
- |
EIF2AK2 |
NNNATGAACAGTGTGCATCG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000012.12 |
12 |
49670186 |
+ |
FMNL3 |
NNNATGAACTGTGTGCATCC |
NGG |
2 |
0.3782 |
Tier I |
| 3 |
NC_000021.9 |
21 |
17550044 |
- |
CXADR |
NNNATGAACTGTGTGCATCT |
NGG |
2 |
0.6176 |
Tier II |
| 4 |
NC_000008.11 |
8 |
39994979 |
- |
IDO2 |
NNNATGAACAGTGTGAATAG |
NGG |
2 |
0.4286 |
Tier II |
| 5 |
NC_000009.12 |
9 |
37630759 |
+ |
FRMPD1 |
NNNATGGACAGTGTGCATCC |
NGG |
2 |
0.3025 |
Tier II |
| 6 |
NC_000001.11 |
1 |
216989936 |
+ |
ESRRG |
NNNATGAACAGTGTGCATGG |
NGG |
1 |
0.125 |
Tier II |
| 7 |
NC_000010.11 |
10 |
55372530 |
+ |
PCDH15 |
NNNATGAACAGTGTGCATGA |
NGG |
2 |
0.1172 |
Tier II |
| 8 |
NC_000017.11 |
17 |
83037689 |
- |
B3GNTL1 |
NNNTTGAACAGTGTGCCTCG |
NGG |
2 |
0.1123 |
Tier II |
| 9 |
NC_000002.12 |
2 |
23492299 |
- |
KLHL29 |
NNNATGAACAGTGTGCAAGG |
NGG |
2 |
0.0833 |
Tier II |
| 10 |
NC_000002.12 |
2 |
96507236 |
- |
NEURL3 |
NNNATGAAGAGTGGGCATCG |
NGG |
2 |
0.0 |
Tier II |
| 11 |
NC_000002.12 |
2 |
23030835 |
+ |
LOC107985792 |
NNNATGAACACTGTGCATCC |
NGG |
2 |
0.1837 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000072.6 |
6 |
76959771 |
+ |
Ctnna2 |
NNNATGAACAGTATGAATCG |
NGG |
2 |
0.9231 |
Tier II |
| 2 |
NC_000077.6 |
11 |
109580350 |
+ |
Wipi1 |
NNNATGAACAGTGAGCATCG |
NGG |
1 |
0.619 |
Tier II |
| 3 |
NC_000081.6 |
15 |
21127961 |
- |
Cdh12 |
NNNATGAACAGTGAGCATCT |
NGG |
2 |
0.4333 |
Tier II |
| 4 |
NC_000073.6 |
7 |
30294457 |
- |
Clip3 |
NNNATGGACAGTGTGCACCG |
NGG |
2 |
0.3025 |
Tier II |
| 5 |
NC_000067.6 |
1 |
127911772 |
+ |
Rab3gap1 |
NNNATGAACAGTGTGTATAG |
NGG |
2 |
0.1319 |
Tier II |
| 6 |
NC_000068.7 |
2 |
33848952 |
+ |
Mvb12b |
NNNATGAACAGTGAGCCTCG |
NGG |
2 |
0.1092 |
Tier II |
| 7 |
NC_000067.6 |
1 |
43151679 |
- |
Fhl2 |
NNNATGAACAGTGTGGATCT |
NGG |
2 |
0.1077 |
Tier II |
| 8 |
NC_000080.6 |
14 |
64866590 |
- |
Hmbox1 |
NNNATGAACATTGTGCATGG |
NGG |
2 |
0.0481 |
Tier II |
| 9 |
NC_000077.6 |
11 |
109580350 |
+ |
Gm39442 |
NNNATGAACAGTGAGCATCG |
NGG |
1 |
0.619 |
Tier III |
| 10 |
NC_000071.6 |
5 |
64482224 |
- |
C030018K13Rik |
NNNAGGAACAGTGTGCATGG |
NGG |
2 |
0.08 |
Tier III |
Other clones with same target sequence:
(none)
