Construct: sgRNA BRDN0001146381

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
GTCCACAAAAAAGACAACTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TP53RK (112858)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77701

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000020.11 20 46687217 + TP53RK NNNCACAAAAAAGACAACTG NGG 0 1.0 Tier I
2 NC_000015.10 15 49637340 - DTWD1 NNNCACCAAAAAGACAAATG NGG 2 0.2356 Tier I
3 NC_000013.11 13 79545741 - NDFIP2 NNNCACAAAAAAAACAACTG NGG 1 0.9231 Tier II
4 NC_000013.11 13 35799248 - DCLK1 NNNCAAAAAAAAAACAACTG NGG 2 0.8571 Tier II
5 NC_000001.11 1 176825940 + PAPPA2 NNNAACAAAAAAGACAACTG NGG 1 0.8421 Tier II
6 NC_000003.12 3 170273845 + PRKCI NNNAAAAAAAAAGACAACTG NGG 2 0.782 Tier II
7 NC_000003.12 3 179356636 - MFN1 NNNAACAAAAAAAACAACTG NGG 2 0.7773 Tier II
8 NC_000009.12 9 85667992 + AGTPBP1 NNNCACAGAAAAGACAACTG NGG 1 0.7333 Tier II
9 NC_000011.10 11 108939150 + DDX10 NNNTACAAATAAGACAACTG NGG 2 0.7059 Tier II
10 NC_000018.10 18 5465718 + EPB41L3 NNNCACAGATAAGACAACTG NGG 2 0.6471 Tier II
11 NC_000019.10 19 16779243 - NWD1 NNNCACAGATAAGACAACTG NGG 2 0.6471 Tier II
12 NC_000009.12 9 125531010 + MAPKAP1 NNNTACAGAAAAGACAACTG NGG 2 0.5867 Tier II
13 NC_000003.12 3 194646303 + LSG1 NNNCACAACAAAGACAACTG NGG 1 0.5714 Tier II
14 NC_000012.12 12 104645533 - CHST11 NNNCAAAATAAAGACAACTG NGG 2 0.5571 Tier II
15 NC_000017.11 17 82571614 + FOXK2 NNNCAAAAAAAAGACAAATG NGG 2 0.5 Tier II
16 NC_000005.10 5 157673005 - C5orf52 NNNGACAAAAAAGACAACTG NGG 1 0.5 Tier II
17 NC_000001.11 1 114742305 + CSDE1 NNNCACAAATAAGACAAATG NGG 2 0.4751 Tier II
18 NC_000015.10 15 50607761 + TRPM7 NNNCCTAAAAAAGACAACTG NGG 2 0.4643 Tier II
19 NC_000019.10 19 5680200 + MICOS13 NNNCACAGAGAAGACAACTG NGG 2 0.4074 Tier II
20 NC_000006.12 6 138867388 + ECT2L NNNCACTAAAAAAACAACTG NGG 2 0.4038 Tier II
21 NC_000009.12 9 3282815 + RFX3 NNNAACAAAAAAGGCAACTG NGG 2 0.393 Tier II
22 NC_000004.12 4 2578494 + FAM193A NNNTACAAAAAAGGCAACTG NGG 2 0.3733 Tier II
23 NC_000014.9 14 34760993 + BAZ1A NNNAACTAAAAAGACAACTG NGG 2 0.3684 Tier II
24 NC_000018.10 18 48265250 - ZBTB7C NNNCACAACAAAGACAATTG NGG 2 0.3673 Tier II
25 NC_000008.11 8 17538250 - SLC7A2 NNNAACAAAAAACACAACTG NGG 2 0.3546 Tier II
26 NC_000004.12 4 93310944 + GRID2 NNNAACAAACAAGACAACTG NGG 2 0.2807 Tier II
27 NC_000013.11 13 98230622 - FARP1 NNNCAGAAAAAAGACAAATG NGG 2 0.2692 Tier II
28 NC_000013.11 13 107624148 + NALF1 NNNTACAAACAAGACAACTG NGG 2 0.2667 Tier II
29 NC_000019.10 19 29815583 - CCNE1 NNNAACAAAATAGACAACTG NGG 2 0.2591 Tier II
30 NC_000002.12 2 205122935 - PARD3B NNNTACAAAAAATACAACTG NGG 2 0.24 Tier II
31 NC_000010.11 10 69356599 + HK1 NNNCACATAAAAGACAAATG NGG 2 0.2308 Tier II
32 NC_000010.11 10 31031344 + ZNF438 NNNCACAAAAAAGAAAACTG NGG 1 0.2222 Tier II
33 NC_000010.11 10 116760661 - HSPA12A NNNCAAAAAAAAGAAAACTG NGG 2 0.2063 Tier II
34 NC_000016.10 16 6503580 - RBFOX1 NNNCACAAATAAGAAAACTG NGG 2 0.1961 Tier II
35 NC_000005.10 5 97162992 + RIOK2 NNNCTCAAAAAAGACAAATG NGG 2 0.1958 Tier II
36 NC_000005.10 5 59052713 - PDE4D NNNTACAAAAAAGAAAACTG NGG 2 0.1778 Tier II
37 NC_000005.10 5 115876161 - AP3S1 NNNTACAAAAAAGAAAACTG NGG 2 0.1778 Tier II
38 NC_000018.10 18 21071854 - ROCK1 NNNTACAAAAAAGAAAACTG NGG 2 0.1778 Tier II
39 NC_000001.11 1 58161716 + DAB1 NNNCACAGAAAAGAAAACTG NGG 2 0.163 Tier II
40 NC_000010.11 10 60998964 - RHOBTB1 NNNCACAGAAAAGAAAACTG NGG 2 0.163 Tier II
41 NC_000016.10 16 20624418 - ACSM1 NNNCACAGAAAAGAAAACTG NGG 2 0.163 Tier II
42 NC_000019.10 19 48456084 - KCNJ14 NNNCACAGAAAAGCCAACTG NGG 2 0.1571 Tier II
43 NC_000019.10 19 48456084 - GRWD1 NNNCACAGAAAAGCCAACTG NGG 2 0.1571 Tier II
44 NC_000019.10 19 14988278 - SLC1A6 NNNCACAAATAAGACAGCTG NGG 2 0.1557 Tier II
45 NC_000003.12 3 108384022 - MYH15 NNNGACAAAATAGACAACTG NGG 2 0.1538 Tier II
46 NC_000010.11 10 23921323 + KIAA1217 NNNCTCAAAACAGACAACTG NGG 2 0.1455 Tier II
47 NC_000012.12 12 106431746 - POLR3B NNNCAGAAAAAAGACAACAG NGG 2 0.1429 Tier II
48 NC_000010.11 10 31017083 + ZNF438 NNNCACACAAAATACAACTG NGG 2 0.1286 Tier II
49 NC_000018.10 18 45507084 - SLC14A2 NNNCACAAAAAAGACAACAC NGG 2 0.1224 Tier II
50 NC_000010.11 10 22650259 - PIP4K2A NNNCAGAAAAAAGAAAACTG NGG 2 0.1111 Tier II
51 NC_000003.12 3 123283844 + ADCY5 NNNTACAAAAAAGACAAGTG NGG 2 0.1067 Tier II
52 NC_000010.11 10 62053763 + ARID5B NNNCACAAAAAAGGAAACTG NGG 2 0.1037 Tier II
53 NC_000020.11 20 35398038 + UQCC1 NNNCACAAAAAAGGAAACTG NGG 2 0.1037 Tier II
54 NC_000005.10 5 45327795 + HCN1 NNNCACAGAAAAGACATCTG NGG 2 0.0978 Tier II
55 NC_000010.11 10 119542350 + RGS10 NNNCACTAAAAAGAAAACTG NGG 2 0.0972 Tier II
56 NC_000004.12 4 183126502 - WWC2 NNNCACAAAAATGACAACAG NGG 2 0.0952 Tier II
57 NC_000003.12 3 25289450 - RARB NNNCACAATAAAGACATCTG NGG 2 0.08 Tier II
58 NC_000017.11 17 27481321 - KSR1 NNNCACAAACAAGAAAACTG NGG 2 0.0741 Tier II
59 NC_000003.12 3 62042741 - PTPRG NNNCACAAGAAAGACAACTG NGA 2 0.0463 Tier II
60 NC_000012.12 12 62038266 + TAFA2 NNNCACAAAAAAGGTAACTG NGG 2 0.0311 Tier II
61 NC_000001.11 1 151139240 - SEMA6C NNNCACAAAAAAGAAAAGTG NGG 2 0.0296 Tier II
62 NC_000003.12 3 174682190 + NAALADL2 NNNCACAAAAAAGAAAAGTG NGG 2 0.0296 Tier II
63 NC_000015.10 15 44634594 - SPG11 NNNCTCAAAAAAGACAACTG NGA 2 0.0253 Tier II
64 NC_000004.12 4 109978185 + EGF NNNCACAAAAATGATAACTG NGG 2 0.0222 Tier II
65 NC_000010.11 10 93774720 - LGI1 NNNCACAAAAAACAGAACTG NGG 2 0.0211 Tier II
66 NC_000021.9 21 17599915 + CXADR NNNCACAAAAATGAGAACTG NGG 2 0.0167 Tier II
67 NC_000021.9 21 17599915 + BTG3 NNNCACAAAAATGAGAACTG NGG 2 0.0167 Tier II
68 NC_000002.12 2 197111280 + ANKRD44 NNNCACAAAAAAGACAAATG NGT 2 0.0087 Tier II
69 NC_000007.14 7 92203149 + KRIT1 NNNCACAAAAAACACAACTG NGT 2 0.0068 Tier II
70 NC_000006.12 6 105349602 + PREP NNNCTCAAAAAAGACAACTG NGT 2 0.0059 Tier II
71 NC_000004.12 4 78083696 - FRAS1 NNNCACAAAAAAGACAACCG NGT 2 0.0044 Tier II
72 NC_000002.12 2 230946395 + GPR55 NNNCACAAAAAAGATAACTG NGT 2 0.0011 Tier II
73 NC_000008.11 8 118325563 - SAMD12 NNNCACAAAAAAGATAACTG NGT 2 0.0011 Tier II
74 NC_000011.10 11 25698835 + LINC02699 NNNAACAAAAAAGACAACTG NGG 1 0.8421 Tier III
75 NC_000003.12 3 194646303 + TMEM44-AS2 NNNCACAACAAAGACAACTG NGG 1 0.5714 Tier III
76 NC_000008.11 8 57978807 - LINC01602 NNNCACAAAGAAAACAACTG NGG 2 0.5128 Tier III
77 NC_000001.11 1 19529510 - LOC105376817 NNNCACAAAAAGGACAACTT NGG 2 0.5056 Tier III
78 NC_000001.11 1 19529510 - LOC105376819 NNNCACAAAAAGGACAACTT NGG 2 0.5056 Tier III
79 NC_000023.11 X 27324919 + LOC105373150 NNNCACAAAAAAGACAACCA NGG 2 0.2586 Tier III
80 NC_000005.10 5 62074642 + LOC124900610 NNNCCCATAAAAGACAACTG NGG 2 0.2143 Tier III
81 NC_000004.12 4 22695758 - GBA3 NNNCACAAATAAGAAAACTG NGG 2 0.1961 Tier III
82 NC_000001.11 1 143361604 - LOC100996736 NNNCTCAAAAAAGACAAATG NGG 2 0.1958 Tier III
83 NC_000001.11 1 144187207 - LOC100996731 NNNCTCAAAAAAGACAAATG NGG 2 0.1958 Tier III
84 NC_000001.11 1 144779187 + LOC100996737 NNNCTCAAAAAAGACAAATG NGG 2 0.1958 Tier III
85 NC_000002.12 2 18967029 - LOC105373456 NNNAACAAAAAAGAAAACTG NGG 2 0.1871 Tier III
86 NC_000006.12 6 145777262 - EPM2A-DT NNNCACAAAAAAGACAACTT NAG 2 0.1815 Tier III
87 NC_000001.11 1 168974779 + LINC00970 NNNCACACAAAACACAACTG NGG 2 0.1805 Tier III
88 NC_000004.12 4 127409370 + LOC102724210 NNNCACAGAAAAGAAAACTG NGG 2 0.163 Tier III
89 NC_000003.12 3 108384022 - LOC124900545 NNNGACAAAATAGACAACTG NGG 2 0.1538 Tier III
90 NC_000008.11 8 23760393 + LOC107986930 NNNCACAAAATTGACAACTG NGG 2 0.1026 Tier III
91 NC_000011.10 11 3634593 - TRPC2 NNNCACAAACAAGACAACAG NGG 2 0.0952 Tier III
92 NC_000018.10 18 56090499 + LINC03069 NNNCACAAATAAGATAACTG NGG 2 0.0588 Tier III
93 NC_000018.10 18 56090499 + LINC01539 NNNCACAAATAAGATAACTG NGG 2 0.0588 Tier III
94 NC_000018.10 18 56090499 + LINC01905 NNNCACAAATAAGATAACTG NGG 2 0.0588 Tier III
95 NC_000002.12 2 52121571 + NRXN1-DT NNNCACATAAAAGACATCTG NGG 2 0.0571 Tier III
96 NC_000002.12 2 34721528 - LINC01320 NNNCACAAAAATGACAACTG NGA 2 0.0231 Tier III
97 NC_000002.12 2 34721528 - LOC105374458 NNNCACAAAAATGACAACTG NGA 2 0.0231 Tier III
98 NC_000010.11 10 93774720 - LOC105378437 NNNCACAAAAAACAGAACTG NGG 2 0.0211 Tier III
99 NC_000004.12 4 66079446 + LOC105377261 NNNCACAAAAAAGACAAATG NTG 2 0.021 Tier III
100 NC_000013.11 13 36708946 - LOC102723490 NNNTACAAAAAAGACAACTG NGC 2 0.0178 Tier III
101 NC_000019.10 19 27824454 + LINC02987 NNNCACAAAAAAGAAAACTG NTG 2 0.0087 Tier III
102 NC_000008.11 8 19204726 + LOC100128993 NNNCACAAAAAAGAAAACTG NGT 2 0.0036 Tier III
103 NC_000008.11 8 118325563 - LOC105375724 NNNCACAAAAAAGATAACTG NGT 2 0.0011 Tier III
104 NC_000004.12 4 79674139 + LINC02469 NNNCACAAAAAAAACCACTG NGG 2 0.0 Tier III
105 NC_000010.11 10 3618751 - LOC105376360 NNNGACAAAAAAGACCACTG NGG 2 0.0 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000067.6 1 54732937 - Ankrd44 NNNCACAAAAAGGACAAATG NGG 2 0.3889 Tier I
2 NC_000073.6 7 62380175 + Magel2 NNNCCCAAAGAAGACAACTG NGG 2 0.2778 Tier I
3 NC_000068.7 2 165491697 + Trp53rka NNNCACAAAGAAGACGACTG NGG 2 0.1068 Tier I
4 NC_000068.7 2 166795397 - Trp53rkb NNNCACAAAGAAGACGACTG NGG 2 0.1068 Tier I
5 NC_000081.6 15 28419923 + Dnah5 NNNCACCAAAAAGACAGCTG NGG 2 0.0772 Tier I
6 NC_000077.6 11 75100076 + Smg6 NNNCAAAAAAAAAACAACTG NGG 2 0.8571 Tier II
7 NC_000068.7 2 156099317 + Rbm12 NNNAAAAAAAAAGACAACTG NGG 2 0.782 Tier II
8 NC_000068.7 2 156099317 + Cpne1 NNNAAAAAAAAAGACAACTG NGG 2 0.782 Tier II
9 NC_000069.6 3 66176985 - Veph1 NNNAAAAAAAAAGACAACTG NGG 2 0.782 Tier II
10 NC_000070.6 4 118170934 + Kdm4a NNNAAAAAAAAAGACAACTG NGG 2 0.782 Tier II
11 NC_000070.6 4 142212819 - Kazn NNNCACAGATAAGACAACTG NGG 2 0.6471 Tier II
12 NC_000072.6 6 87924136 - Hmces NNNCAAAACAAAGACAACTG NGG 2 0.5306 Tier II
13 NC_000071.6 5 51822364 - Ppargc1a NNNCATAACAAAGACAACTG NGG 2 0.5306 Tier II
14 NC_000079.6 13 45741747 - Atxn1 NNNCACAGCAAAGACAACTG NGG 2 0.419 Tier II
15 NC_000068.7 2 55469153 + Kcnj3 NNNAACATAAAAGACAACTG NGG 2 0.3609 Tier II
16 NC_000076.6 10 101825571 + Mgat4c NNNAACAAAAAAGACAACAG NGG 2 0.2406 Tier II
17 NC_000074.6 8 107157754 + Cyb5b NNNCACACAGAAGACAACTG NGG 2 0.2381 Tier II
18 NC_000071.6 5 123662692 - Clip1 NNNCACACAAAAGACAAATG NGG 2 0.2308 Tier II
19 NC_000080.6 14 29507291 - Cacna2d3 NNNCCCTAAAAAGACAACTG NGG 2 0.2188 Tier II
20 NC_000068.7 2 145887622 - Rin2 NNNAACAAAAAAGACAACTG NAG 2 0.2183 Tier II
21 NC_000072.6 6 116301221 + Zfand4 NNNAACAAAAAAGACAACTG NAG 2 0.2183 Tier II
22 NC_000071.6 5 23961237 + Fam126a NNNCCCAAAAAACACAACTG NGG 2 0.2105 Tier II
23 NC_000076.6 10 69805944 + Ank3 NNNCAAAAAAAAGCCAACTG NGG 2 0.199 Tier II
24 NC_000069.6 3 125515240 + Ndst4 NNNAACAAAAAAGAAAACTG NGG 2 0.1871 Tier II
25 NC_000077.6 11 35545774 - Slit3 NNNTACAAAAAAGAAAACTG NGG 2 0.1778 Tier II
26 NC_000067.6 1 120070083 - Tmem37 NNNCACCAAACAGACAACTG NGG 2 0.175 Tier II
27 NC_000079.6 13 30859216 + Exoc2 NNNCATAAAAAAGACAGCTG NGG 2 0.1639 Tier II
28 NC_000080.6 14 33052436 + Wdfy4 NNNAACAAAAAAGACAGCTG NGG 2 0.1486 Tier II
29 NC_000071.6 5 37661157 + Stk32b NNNCACTAAAATGACAACTG NGG 2 0.1458 Tier II
30 NC_000080.6 14 122830360 + Pcca NNNCACAAAAAAGACAAAGG NGG 2 0.1346 Tier II
31 NC_000073.6 7 67618668 + Lrrc28 NNNCAAAAAAAAGACATCTG NGG 2 0.1238 Tier II
32 NC_000077.6 11 35859753 - Wwc1 NNNCACAAAAAAGAAAAATG NGG 2 0.1197 Tier II
33 NC_000083.6 17 12671666 - Slc22a1 NNNCACAAAAAAGAAAAATG NGG 2 0.1197 Tier II
34 NC_000073.6 7 132049535 + Cpxm2 NNNCACAAATAAGACAAGTG NGG 2 0.1176 Tier II
35 NC_000071.6 5 92627981 + Ccdc158 NNNCACAAGAAAGACAGCTG NGG 2 0.1176 Tier II
36 NC_000076.6 10 61624541 - Npffr1 NNNCACACAAAAGACAACTG NAG 2 0.1111 Tier II
37 NC_000069.6 3 19551464 + Dnajc5b NNNGACAAAAAAGAAAACTG NGG 2 0.1111 Tier II
38 NC_000078.6 12 44532895 - Nrcam NNNCACATAAAAGACACCTG NGG 2 0.0756 Tier II
39 NC_000080.6 14 13868119 + Gm281 NNNCACAAAAAAGACAACTA NGA 2 0.0651 Tier II
40 NC_000078.6 12 79210367 + Rdh12 NNNAACAAAAAAGACAACTG NGA 2 0.0585 Tier II
41 NC_000076.6 10 60117659 - Spock2 NNNCACAAAACAGACAAGTG NGG 2 0.0533 Tier II
42 NC_000076.6 10 88391272 + Gnptab NNNCACAAAATAGACAAGTG NGG 2 0.041 Tier II
43 NC_000085.6 19 37109149 + Cpeb3 NNNCACAAAAAAGCCAGCTG NGG 2 0.0378 Tier II
44 NC_000072.6 6 129873781 + Klra17 NNNCACCAAAAAGATAACTG NGG 2 0.0292 Tier II
45 NC_000073.6 7 117648649 + Xylt1 NNNCACAAAAAAGCCAAGTG NGG 2 0.0286 Tier II
46 NC_000080.6 14 93336714 + Pcdh9 NNNCAAAAAAAAGACAACTG NGC 2 0.0206 Tier II
47 NC_000083.6 17 72568764 + Alk NNNCACAAACAAGAGAACTG NGG 2 0.0167 Tier II
48 NC_000074.6 8 58619414 - Galntl6 NNNCACAAAAAAGACAGCTG NGA 2 0.0123 Tier II
49 NC_000067.6 1 39557227 + Rnf149 NNNCTCAAAAAAGACAACTG NGC 2 0.0081 Tier II
50 NC_000071.6 5 17602214 - Sema3c NNNCACAAAAAAGACTACTG NGC 2 0.0 Tier II
51 NC_000075.6 9 32120980 - Arhgap32 NNNCACACAAAAGACCACTG NGG 2 0.0 Tier II
52 NC_000082.6 16 14990741 - Gm46561 NNNCAAAAGAAAGACAACTG NGG 2 0.619 Tier III
53 NC_000067.6 1 54732937 - 4930444A19Rik NNNCACAAAAAGGACAAATG NGG 2 0.3889 Tier III
54 NC_000080.6 14 8395760 + Gm41095 NNNCACAAAATAGACAACTG NGG 1 0.3077 Tier III
55 NC_000071.6 5 39308702 - Gm40292 NNNAACAAACAAGACAACTG NGG 2 0.2807 Tier III
56 NC_000073.6 7 62380175 + Gm32061 NNNCCCAAAGAAGACAACTG NGG 2 0.2778 Tier III
57 NC_000074.6 8 128295693 - A930035F09Rik NNNCACAAAAGACACAACTG NGG 2 0.2737 Tier III
58 NC_000079.6 13 52998663 - Gm33424 NNNCACAGACAAGACAACTG NGG 2 0.2444 Tier III
59 NC_000068.7 2 145887622 - Gm39954 NNNAACAAAAAAGACAACTG NAG 2 0.2183 Tier III
60 NC_000078.6 12 80126833 + 2310015A10Rik NNNCACAAAAAAGACAACCC NGG 2 0.1182 Tier III
61 NC_000068.7 2 65407553 + Gm34739 NNNCACAAAAGAGACATCTG NGG 2 0.0867 Tier III
62 NC_000073.6 7 142110417 + Gm32786 NNNCACAAAGAAGACAAGTG NGG 2 0.0741 Tier III
63 NC_000085.6 19 27204352 - Gm35438 NNNCAAAAAAAAGATAACTG NGG 2 0.0619 Tier III
64 NC_000076.6 10 60117659 - Gm51801 NNNCACAAAACAGACAAGTG NGG 2 0.0533 Tier III
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Other clones with same target sequence:

(none)