Construct: sgRNA BRDN0001146382

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
CTTACTCTCTGTCAATCACA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PRKG1 (5592)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77004

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000010.11 10 52133894 - PRKG1 NNNACTCTCTGTCAATCACA NGG 0 1.0 Tier I
2 NC_000010.11 10 5949200 - IL15RA NNNGCTCTCTGTCAATTACA NGG 2 0.2917 Tier I
3 NC_000002.12 2 215066280 + ABCA12 NNNACTCTCTGTCAAACACA NGG 1 0.9091 Tier II
4 NC_000005.10 5 127442255 - MEGF10 NNNACTCTCTGTCAAACACA NGG 1 0.9091 Tier II
5 NC_000009.12 9 121817203 + TTLL11 NNNACACTCTGCCAATCACA NGG 2 0.8211 Tier II
6 NC_000004.12 4 101113022 - PPP3CA NNNAATCTCTATCAATCACA NGG 2 0.5714 Tier II
7 NC_000023.11 X 6899145 + PUDP NNNAATTTCTGTCAATCACA NGG 2 0.4643 Tier II
8 NC_000003.12 3 172097110 - FNDC3B NNNACTCGCTGTCAATCAAA NGG 2 0.3143 Tier II
9 NC_000010.11 10 5952687 - IL15RA NNNGCTCTCTGTCAATTACA NGG 2 0.2917 Tier II
10 NC_000004.12 4 80466957 + CFAP299 NNNCCTCTCTGTCAATTACA NGG 2 0.1647 Tier II
11 NC_000001.11 1 12260937 - VPS13D NNNACTCTGTGTCAATCACA NAG 2 0.1605 Tier II
12 NC_000009.12 9 130704102 + EXOSC2 NNNACTCACTGTCATTCACA NGG 2 0.16 Tier II
13 NC_000018.10 18 74319253 - C18orf63 NNNACTCTCTGTCAATTACC NGG 2 0.1061 Tier II
14 NC_000002.12 2 143296228 + ARHGAP15 NNNCCTCTCTGTCAATCACA NAG 2 0.0915 Tier II
15 NC_000021.9 21 42734437 + PDE9A NNNACTCTTTGTCAATGACA NGG 2 0.0515 Tier II
16 NC_000009.12 9 87588645 + DAPK1 NNNACTCTCTGTCACTCAGA NGG 2 0.0341 Tier II
17 NC_000007.14 7 78477215 - MAGI2 NNNAGTCTCTGTCAATCACA NTG 2 0.0234 Tier II
18 NC_000005.10 5 76108144 - SV2C NNNACTCTCTGTCAATCACA NGT 1 0.0161 Tier II
19 NC_000009.12 9 23914299 - LOC105375993 NNNACTCTCTATCAATCTCA NGG 2 0.5 Tier III
20 NC_000002.12 2 23108521 + LOC107985792 NNNACTCTCTGTCAGCCACA NGG 2 0.4333 Tier III
21 NC_000016.10 16 80108928 - LOC105371357 NNNACTCTCTGCCAAGCACA NGG 2 0.3279 Tier III
22 NC_000016.10 16 80108928 - LOC105371358 NNNACTCTCTGCCAAGCACA NGG 2 0.3279 Tier III
23 NC_000021.9 21 42734437 + PDE9A-AS1 NNNACTCTTTGTCAATGACA NGG 2 0.0515 Tier III
24 NC_000004.12 4 79581352 + LOC107986294 NNNACTCTCTGTCAATGACA NAG 2 0.0153 Tier III
25 NC_000004.12 4 147014940 + LOC105377475 NNNACTCTCTGTCAATCACA NCT 2 0.0 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000085.6 19 30724172 + Prkg1 NNNACTCTCTGTCAATCACA NGG 0 1.0 Tier I
2 NC_000068.7 2 122069765 + Spg11 NNNACTCATTGTCAATCACA NGG 2 0.7 Tier II
3 NC_000075.6 9 117296446 + Rbms3 NNNGCTCTCTGTCAAACACA NGG 2 0.5682 Tier II
4 NC_000080.6 14 23842584 - Kcnma1 NNNACTCACTGTCAACCACA NGG 2 0.5333 Tier II
5 NC_000082.6 16 24859131 + Lpp NNNACTTTCTGTCAGTCACA NGG 2 0.5281 Tier II
6 NC_000079.6 13 76560532 - Mctp1 NNNTCTCTCTGACAATCACA NGG 2 0.5091 Tier II
7 NC_000068.7 2 88934050 - Olfr1211 NNNACTCTCTGACAATCACT NGG 2 0.48 Tier II
8 NC_000078.6 12 52852538 - Akap6 NNNACACTCTGTCTATCACA NGG 2 0.4622 Tier II
9 NC_000085.6 19 34813293 + Pank1 NNNAATCACTGTCAATCACA NGG 2 0.4571 Tier II
10 NC_000068.7 2 32172740 + Prrc2b NNNACTCTCTGTCTACCACA NGG 2 0.3556 Tier II
11 NC_000071.6 5 44616634 + Ldb2 NNNACTCTCTGTCAACCTCA NGG 2 0.3333 Tier II
12 NC_000070.6 4 118037495 + St3gal3 NNNAATCTCTGGCAATCACA NGG 2 0.2857 Tier II
13 NC_000071.6 5 13319736 + Sema3a NNNACTTTCTGTCAAGCACA NGG 2 0.2813 Tier II
14 NC_000083.6 17 26041525 + Rab11fip3 NNNACTCTCTGTCAAACACA NAG 2 0.2357 Tier II
15 NC_000070.6 4 55049694 - Zfp462 NNNCCTCTCTGTCAGTCACA NGG 2 0.2294 Tier II
16 NC_000068.7 2 14867325 + Cacnb2 NNNAGTCTCTGTCAATCACA NAG 2 0.1556 Tier II
17 NC_000080.6 14 65994992 + Gulo NNNACTCCCTGTCAATCACA NCG 2 0.1071 Tier II
18 NC_000081.6 15 10546727 + Ttc23l NNNACTCTCTGTGAACCACA NGG 2 0.0909 Tier II
19 NC_000070.6 4 130266661 + Serinc2 NNNACTCTCTGTCACTCACA NAG 2 0.0707 Tier II
20 NC_000079.6 13 117732827 - Hcn1 NNNACTCTCTTTCAATCAGA NGG 2 0.0481 Tier II
21 NC_000083.6 17 55843092 - Adgre4 NNNACTCTCTGTCAATCTCA NTG 2 0.0195 Tier II
22 NC_000079.6 13 8605072 + Adarb2 NNNACTCACTGTCAATCACA NGC 2 0.0178 Tier II
23 NC_000068.7 2 143749774 - Pcsk2 NNNAGTCTCTGTCAATCACA NGT 2 0.0097 Tier II
24 NC_000084.6 18 13993181 - 6330525I24Rik NNNACCCTCTGTCCATCACA NGG 2 0.1948 Tier III
25 NC_000069.6 3 138000809 + Gm19708 NNNTCTCTCTGTCAATCACA NAG 2 0.165 Tier III
26 NC_000067.6 1 64254927 - Gm39654 NNNACTCTCTCTCATTCACA NGG 2 0.0857 Tier III
27 NC_000068.7 2 143749774 - Pcsk2os2 NNNAGTCTCTGTCAATCACA NGT 2 0.0097 Tier III
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Other clones with same target sequence:

(none)