Construct: sgRNA BRDN0001146383

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
AGGAAATAATAACTTTGTCA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GK (2710)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76329

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000023.11 X 30691195 + GK NNNAAATAATAACTTTGTCA NGG 0 1.0 Tier I
2 NC_000003.12 3 57277900 - ASB14 NNNAAGTAATAACTTTGTCA NGG 1 0.7143 Tier I
3 NC_000004.12 4 165279237 - GK3 NNNAAATAATAACTTTGTCA NGT 1 0.0161 Tier I
4 NC_000015.10 15 73057580 + NEO1 NNNAAAAATTAACTTTGTCA NGG 2 0.525 Tier II
5 NC_000002.12 2 143035074 - KYNU NNNAAATAAAAACTTTGTCT NGG 2 0.5143 Tier II
6 NC_000001.11 1 97779149 + DPYD NNNAAATAATAATTTTGACA NGG 2 0.4667 Tier II
7 NC_000014.9 14 52646823 + ERO1A NNNACAAAATAACTTTGTCA NGG 2 0.4375 Tier II
8 NC_000003.12 3 173734802 - NLGN1 NNNAGATATTAACTTTGTCA NGG 2 0.432 Tier II
9 NC_000010.11 10 115549350 - ATRNL1 NNNAAGTAATAACTTTGTCT NGG 2 0.4286 Tier II
10 NC_000002.12 2 182614196 + PDE1A NNNAACTAATAACTTAGTCA NGG 2 0.4132 Tier II
11 NC_000011.10 11 21406100 + NELL1 NNNAAATAAAAACTTTGTAA NGG 2 0.3673 Tier II
12 NC_000004.12 4 22439844 + ADGRA3 NNNACATAGTAACTTTGTCA NGG 2 0.3333 Tier II
13 NC_000012.12 12 43926742 + TMEM117 NNNAAATAATATCTTTATCA NGG 2 0.3111 Tier II
14 NC_000001.11 1 233661189 - KCNK1 NNNAAATAATAACTTAGGCA NGG 2 0.303 Tier II
15 NC_000006.12 6 163019206 + PACRG NNNAAATAATAATTTTGTAA NGG 2 0.3 Tier II
16 NC_000003.12 3 55005831 - CACNA2D3 NNNAAATAATAACATTGTAA NGG 2 0.2653 Tier II
17 NC_000007.14 7 702038 + PRKAR1B NNNAAATTATAACATTGTCA NGG 2 0.2653 Tier II
18 NC_000005.10 5 172434741 - SH3PXD2B NNNATGTAATAACTTTGTCA NGG 2 0.2597 Tier II
19 NC_000012.12 12 92796719 + EEA1 NNNCAATGATAACTTTGTCA NGG 2 0.2588 Tier II
20 NC_000002.12 2 149024079 - KIF5C NNNAAATAATAACTTATTCA NGG 2 0.2273 Tier II
21 NC_000013.11 13 93997441 + GPC6 NNNAAATAATGACTTGGTCA NGG 2 0.225 Tier II
22 NC_000008.11 8 138637696 + COL22A1 NNNAAATAGTATCTTTGTCA NGG 2 0.2222 Tier II
23 NC_000013.11 13 26379399 - CDK8 NNNAAATAGTATCTTTGTCA NGG 2 0.2222 Tier II
24 NC_000003.12 3 154383591 - GPR149 NNNAAATCAGAACTTTGTCA NGG 2 0.2143 Tier II
25 NC_000001.11 1 26443924 - DHDDS NNNTAATAATATCTTTGTCA NGG 2 0.2121 Tier II
26 NC_000003.12 3 69258837 - FRMD4B NNNAAAAAATAACTTTCTCA NGG 2 0.2059 Tier II
27 NC_000006.12 6 118002672 + SLC35F1 NNNAAATAATAATTTTTTCA NGG 2 0.175 Tier II
28 NC_000002.12 2 222220849 + PAX3 NNNAAATAATGACTTTGTCA NAG 2 0.1685 Tier II
29 NC_000014.9 14 91109166 - DGLUCY NNNAAATAATACCATTGTCA NGG 2 0.1629 Tier II
30 NC_000008.11 8 42482292 - SLC20A2 NNNTAATAATAACTTTTTCA NGG 2 0.1591 Tier II
31 NC_000006.12 6 159222842 - FNDC1 NNNAAATAATAACATTTTCA NGG 2 0.1548 Tier II
32 NC_000004.12 4 86992657 - AFF1 NNNCAATTATAACTTTGTCA NGG 2 0.1513 Tier II
33 NC_000004.12 4 155185880 + NPY2R NNNAAATATTAACTTTTTCA NGG 2 0.15 Tier II
34 NC_000002.12 2 27868998 - RBKS NNNTAATAATAACTTTCTCA NGG 2 0.1497 Tier II
35 NC_000010.11 10 85658387 - GRID1 NNNAAATAATAACTTTATGA NGG 2 0.1167 Tier II
36 NC_000007.14 7 95057030 - PPP1R9A NNNAAATAATACATTTGTCA NGG 2 0.1012 Tier II
37 NC_000009.12 9 10401285 + PTPRD NNNAAATAATAAATTTTTCA NGG 2 0.0962 Tier II
38 NC_000003.12 3 88114479 + CGGBP1 NNNAAATAATAAATTTCTCA NGG 2 0.0905 Tier II
39 NC_000003.12 3 88114479 + ZNF654 NNNAAATAATAAATTTCTCA NGG 2 0.0905 Tier II
40 NC_000001.11 1 156731423 - METTL25B NNNAAATAATAACTTTGGCC NGG 2 0.0758 Tier II
41 NC_000002.12 2 63176082 - WDPCP NNNAAATAACAAGTTTGTCA NGG 2 0.0727 Tier II
42 NC_000009.12 9 39259016 + CNTNAP3 NNNAAATACTAACTTTGTGA NGG 2 0.0714 Tier II
43 NC_000009.12 9 42096876 + CNTNAP3B NNNAAATACTAACTTTGTGA NGG 2 0.0714 Tier II
44 NC_000009.12 9 61360019 - CNTNAP3C NNNAAATACTAACTTTGTGA NGG 2 0.0714 Tier II
45 NC_000003.12 3 165064121 + SI NNNAAATAATAACTTAGTCA NGA 2 0.0631 Tier II
46 NC_000011.10 11 101564432 - TRPC6 NNNAAATAATAAGTTTGTAA NGG 2 0.0584 Tier II
47 NC_000002.12 2 157313796 - GALNT5 NNNATATAATAAGTTTGTCA NGG 2 0.0496 Tier II
48 NC_000005.10 5 11119673 + CTNND2 NNNAAATAATAAGTTTGTCA NAG 2 0.0354 Tier II
49 NC_000001.11 1 243592789 + AKT3 NNNAAATAGTAACTGTGTCA NGG 2 0.0333 Tier II
50 NC_000003.12 3 17686383 + TBC1D5 NNNAAATAATAACTTTGTGA NAG 2 0.0324 Tier II
51 NC_000008.11 8 119218042 + MAL2 NNNGAATAATAACTGTGTCA NGG 2 0.0313 Tier II
52 NC_000007.14 7 121370474 - FAM3C NNNAAATAATAAATTTGTCA NGA 2 0.0267 Tier II
53 NC_000011.10 11 96135196 + MAML2 NNNAAATAATAACTTTGTCT NTG 2 0.0234 Tier II
54 NC_000001.11 1 215945441 + USH2A NNNAAATAATATCTTTGTCA NGA 2 0.0231 Tier II
55 NC_000005.10 5 37551433 - WDR70 NNNAAATAATAACTTTTTCA NGA 2 0.0174 Tier II
56 NC_000007.14 7 90886772 - CDK14 NNNAAATAATAACTTTCTCA NGA 2 0.0163 Tier II
57 NC_000004.12 4 165279237 - KLHL2 NNNAAATAATAACTTTGTCA NGT 1 0.0161 Tier II
58 NC_000017.11 17 1390268 - YWHAE NNNAAATAATAATTTTGTCA NGT 2 0.0113 Tier II
59 NC_000017.11 17 60397225 + USP32 NNNTAATAATAACTTTGTCA NGT 2 0.0103 Tier II
60 NC_000007.14 7 32304871 - PDE1C NNNAAATAATAACATTGTCA NGT 2 0.01 Tier II
61 NC_000004.12 4 161784567 - FSTL5 NNNAAATAATAACTTTCTCA NTG 2 0.0092 Tier II
62 NC_000003.12 3 121028288 + STXBP5L NNNAAATAATAACTTTTTCA NGC 2 0.0056 Tier II
63 NC_000018.10 18 61370601 - CDH20 NNNAAATAATAACTTTGTGA NGT 2 0.002 Tier II
64 NC_000003.12 3 57277900 - LOC105377102 NNNAAGTAATAACTTTGTCA NGG 1 0.7143 Tier III
65 NC_000003.12 3 173734802 - LOC105374225 NNNAGATATTAACTTTGTCA NGG 2 0.432 Tier III
66 NC_000005.10 5 128708465 - LOC105379168 NNNAAATAATAACTTTGTTG NGG 2 0.3529 Tier III
67 NC_000023.11 X 33821926 + LOC105373153 NNNAAAAAATAACTTTGTCA NAG 2 0.2269 Tier III
68 NC_000003.12 3 103993704 + LOC124909491 NNNAAATAGTAACTCTGTCA NGG 2 0.1818 Tier III
69 NC_000012.12 12 68684370 - LOC100507250 NNNAAACAATACCTTTGTCA NGG 2 0.1809 Tier III
70 NC_000002.12 2 222220849 + LOC107985991 NNNAAATAATGACTTTGTCA NAG 2 0.1685 Tier III
71 NC_000009.12 9 41427825 + LOC107984035 NNNAAATAATAACTTTTACA NGG 2 0.1667 Tier III
72 NC_000009.12 9 41427825 + LOC124900272 NNNAAATAATAACTTTTACA NGG 2 0.1667 Tier III
73 NC_000002.12 2 212951069 - LOC102725082 NNNAACTAATATCTTTGTCA NGG 2 0.1515 Tier III
74 NC_000012.12 12 58720278 - LOC100506869 NNNAAATAATAAATTTGGCA NGG 2 0.1282 Tier III
75 NC_000012.12 12 58720278 - LINC02388 NNNAAATAATAAATTTGGCA NGG 2 0.1282 Tier III
76 NC_000018.10 18 14636812 + LOC105372004 NNNAAATAATAAATTTGGCA NGG 2 0.1282 Tier III
77 NC_000005.10 5 114560399 - LOC101927078 NNNAAATAATAACTTAGTGA NGG 2 0.1136 Tier III
78 NC_000002.12 2 56043208 + MIR217HG NNNAAATAATAACTTTCTTA NGG 2 0.1086 Tier III
79 NC_000002.12 2 56043208 + LOC105374690 NNNAAATAATAACTTTCTTA NGG 2 0.1086 Tier III
80 NC_000007.14 7 95057030 - PPP1R9A-AS1 NNNAAATAATACATTTGTCA NGG 2 0.1012 Tier III
81 NC_000001.11 1 71176519 - ZRANB2-DT NNNAAATAATAAATTTCTCA NGG 2 0.0905 Tier III
82 NC_000009.12 9 67090567 - CNTNAP3P2 NNNAAATACTAACTTTGTGA NGG 2 0.0714 Tier III
83 NC_000008.11 8 119218042 + MAL2-AS1 NNNGAATAATAACTGTGTCA NGG 2 0.0313 Tier III
84 NC_000015.10 15 36468176 - LOC105370768 NNNAAATAATAATTTTGTCA NTG 2 0.0273 Tier III
85 NC_000021.9 21 16210263 + MIR99AHG NNNAAATAATAAGTTTGTGA NGG 2 0.017 Tier III
86 NC_000004.12 4 119394549 - GK6P NNNAAATAATAACTTTGTCA NGT 1 0.0161 Tier III
87 NC_000004.12 4 119394549 - LOC107986193 NNNAAATAATAACTTTGTCA NGT 1 0.0161 Tier III
88 NC_000023.11 X 101780132 - GK4P NNNAAATAATAACTTTGTCA NGT 1 0.0161 Tier III
89 NC_000007.14 7 13719929 - LOC105375161 NNNAAATAATTACTTTGTCA NTG 2 0.012 Tier III
90 NC_000021.9 21 10050919 - LOC105372733 NNNAAATAATAACTTTTTCA NTG 2 0.0097 Tier III
91 NT_187374.1 9 20976 - LOC107987370 NNNAAATAATAACTTTTTCA NTG 2 0.0097 Tier III
92 NC_000017.11 17 62814668 - MARCHF10-DT NNNAAATAATAACTTGGTCA NGT 2 0.0056 Tier III
93 NC_000003.12 3 34914795 + LOC101928135 NNNAAATAATAACTTTGTCA NTC 2 0.0 Tier III
94 NC_000008.11 8 95684825 - CFAP418-AS1 NNNAAATAATAACTTTGTCA NAC 2 0.0 Tier III
95 NC_000014.9 14 86118911 - LINC02328 NNNAAATAATAACTTTGTCA NCT 2 0.0 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000086.7 X 85746230 - Gk NNNAAATAATAACTTTGTCA NGG 0 1.0 Tier I
2 NC_000080.6 14 26914198 + Asb14 NNNAAGTCATAACTTTGTCA NGG 2 0.3061 Tier I
3 NC_000074.6 8 93119156 - Ces1c NNNAAATATTAACTTTGTCA NGG 1 0.6 Tier II
4 NC_000067.6 1 123621454 + Dpp10 NNNAAATAATAACATAGTCA NGG 2 0.5628 Tier II
5 NC_000080.6 14 57534016 + Il17d NNNAAATAAAAACATTGTCA NGG 2 0.5306 Tier II
6 NC_000073.6 7 133828838 + Fank1 NNNAAATAGTAGCTTTGTCA NGG 2 0.4815 Tier II
7 NC_000067.6 1 60754758 + Cd28 NNNAATTAATGACTTTGTCA NGG 2 0.4643 Tier II
8 NC_000072.6 6 61286157 - Ccser1 NNNAACTAATAACTTTGTCA NGG 1 0.4545 Tier II
9 NC_000067.6 1 24061681 - Fam135a NNNAAACAATAACTTTGTCT NGG 2 0.4125 Tier II
10 NC_000085.6 19 58792899 + 1700019N19Rik NNNAAAGAATGACTTTGTCA NGG 2 0.3824 Tier II
11 NC_000085.6 19 37582449 + Exoc6 NNNAAATAATCACTTTATCA NGG 2 0.3733 Tier II
12 NC_000068.7 2 40760489 - Lrp1b NNNAAATAATAAATTTATCA NGG 2 0.359 Tier II
13 NC_000071.6 5 7011378 + Zfp804b NNNCAATAATAACTTTGTCA NGG 1 0.3529 Tier II
14 NC_000086.7 X 161233202 - Scml2 NNNAAATAATAAATTAGTCA NGG 2 0.3497 Tier II
15 NC_000086.7 X 50874864 - Stk26 NNNAAAGACTAACTTTGTCA NGG 2 0.3361 Tier II
16 NC_000076.6 10 4958669 - Esr1 NNNAAATAATATCTTTGTCA NGG 1 0.3333 Tier II
17 NC_000073.6 7 126887221 + Tmem219 NNNAAATAATAATTTTGCCA NGG 2 0.3 Tier II
18 NC_000067.6 1 124255211 + Dpp10 NNNCAATGATAACTTTGTCA NGG 2 0.2588 Tier II
19 NC_000068.7 2 16062181 + Malrd1 NNNCAATGATAACTTTGTCA NGG 2 0.2588 Tier II
20 NC_000069.6 3 15382879 + Sirpb1a NNNCAATGATAACTTTGTCA NGG 2 0.2588 Tier II
21 NC_000069.6 3 15506883 + Sirpb1b NNNCAATGATAACTTTGTCA NGG 2 0.2588 Tier II
22 NC_000069.6 3 15806261 + Sirpb1c NNNCAATGATAACTTTGTCA NGG 2 0.2588 Tier II
23 NC_000072.6 6 8387600 - Umad1 NNNCAATGATAACTTTGTCA NGG 2 0.2588 Tier II
24 NC_000072.6 6 64185232 - Grid2 NNNCAATGATAACTTTGTCA NGG 2 0.2588 Tier II
25 NC_000073.6 7 7224607 + Vmn2r29 NNNCAATGATAACTTTGTCA NGG 2 0.2588 Tier II
26 NC_000077.6 11 9281827 - Abca13 NNNCAATGATAACTTTGTCA NGG 2 0.2588 Tier II
27 NC_000079.6 13 14301750 - Hecw1 NNNCAATGATAACTTTGTCA NGG 2 0.2588 Tier II
28 NC_000079.6 13 104326275 - Adamts6 NNNCAATGATAACTTTGTCA NGG 2 0.2588 Tier II
29 NC_000080.6 14 30741573 - Sfmbt1 NNNCAATGATAACTTTGTCA NGG 2 0.2588 Tier II
30 NC_000082.6 16 16015225 - Spidr NNNCAATGATAACTTTGTCA NGG 2 0.2588 Tier II
31 NC_000084.6 18 14929803 - Taf4b NNNCAATGATAACTTTGTCA NGG 2 0.2588 Tier II
32 NC_000084.6 18 31166509 - Rit2 NNNCAATGATAACTTTGTCA NGG 2 0.2588 Tier II
33 NC_000085.6 19 32081660 + Asah2 NNNCAATGATAACTTTGTCA NGG 2 0.2588 Tier II
34 NC_000086.7 X 141604818 + Col4a5 NNNCAATGATAACTTTGTCA NGG 2 0.2588 Tier II
35 NC_000086.7 X 144978223 + Rtl4 NNNCAATGATAACTTTGTCA NGG 2 0.2588 Tier II
36 NC_000067.6 1 21516397 + Kcnq5 NNNAAATGATAACTTGGTCA NGG 2 0.2538 Tier II
37 NC_000068.7 2 15911011 - Malrd1 NNNAAATGATAACTTGGTCA NGG 2 0.2538 Tier II
38 NC_000068.7 2 112580248 - Aven NNNAAATGATAACTTGGTCA NGG 2 0.2538 Tier II
39 NC_000069.6 3 119268270 - Dpyd NNNAAATGATAACTTGGTCA NGG 2 0.2538 Tier II
40 NC_000072.6 6 84994451 - Exoc6b NNNAAATGATAACTTGGTCA NGG 2 0.2538 Tier II
41 NC_000076.6 10 83271567 - Slc41a2 NNNAAATGATAACTTGGTCA NGG 2 0.2538 Tier II
42 NC_000079.6 13 10226745 + Chrm3 NNNAAATGATAACTTGGTCA NGG 2 0.2538 Tier II
43 NC_000079.6 13 104439542 - Adamts6 NNNAAATGATAACTTGGTCA NGG 2 0.2538 Tier II
44 NC_000086.7 X 101094627 - Snx12 NNNAAATGATAACTTGGTCA NGG 2 0.2538 Tier II
45 NC_000079.6 13 36102382 - Lyrm4 NNNAAATAATAATTTGGTCA NGG 2 0.2423 Tier II
46 NC_000076.6 10 33119420 - Trdn NNNCAATAATAACTTCGTCA NGG 2 0.2353 Tier II
47 NC_000073.6 7 17914547 - Gm5155 NNNTAATAATAACTTGGTCA NGG 2 0.2203 Tier II
48 NC_000086.7 X 138671356 - Il1rapl2 NNNTAATAATAACTTGGTCA NGG 2 0.2203 Tier II
49 NC_000072.6 6 106789503 - Crbn NNNAAATGATAACTCTGTCA NGG 2 0.2 Tier II
50 NC_000084.6 18 53926407 + Csnk1g3 NNNTAATAATTACTTTGTCA NGG 2 0.1958 Tier II
51 NC_000080.6 14 17765546 - Thrb NNNAAATGATACCTTTGTCA NGG 2 0.193 Tier II
52 NC_000071.6 5 134720908 + Eln NNNAAATAATGACTCTGTCA NGG 2 0.1773 Tier II
53 NC_000074.6 8 85374015 - Mylk3 NNNAAATACTTACTTTGTCA NGG 2 0.1758 Tier II
54 NC_000081.6 15 58074800 + Zhx1 NNNAAATAATGACTTTTTCA NGG 2 0.1625 Tier II
55 NC_000072.6 6 102272271 + Cntn3 NNNAAATATTAACTTTGTCA NAG 2 0.1556 Tier II
56 NC_000076.6 10 115579235 + Lgr5 NNNAAATAATATCTTTGTTA NGG 2 0.1538 Tier II
57 NC_000076.6 10 115579235 + A930009A15Rik NNNAAATAATATCTTTGTTA NGG 2 0.1538 Tier II
58 NC_000080.6 14 59958460 - Atp8a2 NNNAAAGAATAACTTTCTCA NGG 2 0.1384 Tier II
59 NC_000067.6 1 6515253 - St18 NNNCAATAATAACTTGGTCA NGG 2 0.1222 Tier II
60 NC_000067.6 1 20385478 + Pkhd1 NNNCAATAATAACTTGGTCA NGG 2 0.1222 Tier II
61 NC_000067.6 1 70332339 + Spag16 NNNCAATAATAACTTGGTCA NGG 2 0.1222 Tier II
62 NC_000068.7 2 77537530 - Zfp385b NNNCAATAATAACTTGGTCA NGG 2 0.1222 Tier II
63 NC_000068.7 2 137053100 - Slx4ip NNNCAATAATAACTTGGTCA NGG 2 0.1222 Tier II
64 NC_000069.6 3 84121715 - Mnd1 NNNCAATAATAACTTGGTCA NGG 2 0.1222 Tier II
65 NC_000070.6 4 14910455 - Pip4p2 NNNCAATAATAACTTGGTCA NGG 2 0.1222 Tier II
66 NC_000071.6 5 17668507 - Sema3c NNNCAATAATAACTTGGTCA NGG 2 0.1222 Tier II
67 NC_000071.6 5 109431041 - Vmn2r17 NNNCAATAATAACTTGGTCA NGG 2 0.1222 Tier II
68 NC_000073.6 7 107152215 + Nlrp14 NNNCAATAATAACTTGGTCA NGG 2 0.1222 Tier II
69 NC_000074.6 8 68304171 - Sh2d4a NNNCAATAATAACTTGGTCA NGG 2 0.1222 Tier II
70 NC_000076.6 10 33205672 - Trdn NNNCAATAATAACTTGGTCA NGG 2 0.1222 Tier II
71 NC_000077.6 11 29940413 + Eml6 NNNCAATAATAACTTGGTCA NGG 2 0.1222 Tier II
72 NC_000077.6 11 105784118 + Tanc2 NNNCAATAATAACTTGGTCA NGG 2 0.1222 Tier II
73 NC_000078.6 12 37925656 - Dgkb NNNCAATAATAACTTGGTCA NGG 2 0.1222 Tier II
74 NC_000081.6 15 21525464 - Cdh12 NNNCAATAATAACTTGGTCA NGG 2 0.1222 Tier II
75 NC_000082.6 16 11732933 - Snx29 NNNCAATAATAACTTGGTCA NGG 2 0.1222 Tier II
76 NC_000083.6 17 90487679 + Nrxn1 NNNCAATAATAACTTGGTCA NGG 2 0.1222 Tier II
77 NC_000086.7 X 43064631 + Tenm1 NNNCAATAATAACTTGGTCA NGG 2 0.1222 Tier II
78 NC_000074.6 8 49317753 - Tenm3 NNNAAATAAAAAGTTTGTCA NGG 2 0.1169 Tier II
79 NC_000083.6 17 20354144 - Vmn2r107 NNNAAATAATAACTTTGTAA NAG 2 0.1111 Tier II
80 NC_000074.6 8 90255120 + Tox3 NNNAAATAATAACTTTCTAA NGG 2 0.1008 Tier II
81 NC_000079.6 13 93840555 + Arsb NNNAAATAATTACCTTGTCA NGG 2 0.0879 Tier II
82 NC_000078.6 12 75357796 + Rhoj NNNAAATAATAACTTGTTCA NGG 2 0.0865 Tier II
83 NC_000075.6 9 58626424 + Nptn NNNAAATAATACCTTTTTCA NGG 2 0.0658 Tier II
84 NC_000067.6 1 50934362 - Tmeff2 NNNAAATAATAACTTTTTCA NAG 2 0.0648 Tier II
85 NC_000078.6 12 112009405 - Tdrd9 NNNAAATAATAACTTTTTCA NAG 2 0.0648 Tier II
86 NC_000079.6 13 43257751 + Gfod1 NNNACATAATAACTTTGTCA NGA 2 0.0347 Tier II
87 NC_000072.6 6 40447641 + Wee2 NNNAAACAATAACTTTGTCA NTG 2 0.0268 Tier II
88 NC_000078.6 12 61608646 - Lrfn5 NNNAAATTATAACTTTGTCA NTG 2 0.0167 Tier II
89 NC_000082.6 16 67499307 + Cadm2 NNNAAATAATAACATTGTCA NGT 2 0.01 Tier II
90 NC_000082.6 16 97893993 + C2cd2 NNNAAATAATTACTTTGTCA NGC 2 0.0068 Tier II
91 NC_000086.7 X 35776018 - Gm39502 NNNAAATGATAACTTAGTCA NGG 2 0.6667 Tier III
92 NC_000073.6 7 120842203 - Gm36449 NNNAATTAATAACTTTATCA NGG 2 0.6667 Tier III
93 NC_000070.6 4 24003371 - Gm27243 NNNAAATAATAATTTTATCA NGG 2 0.6533 Tier III
94 NC_000069.6 3 126173742 - Gm40146 NNNTAATGATAACTTTGTCA NGG 2 0.4667 Tier III
95 NC_000067.6 1 33324628 + Gm39617 NNNAAATACTAACTTTGACA NGG 2 0.381 Tier III
96 NC_000072.6 6 141905286 + Gm30843 NNNCAATGATAACTTTGTCA NGG 2 0.2588 Tier III
97 NC_000076.6 10 24428392 + Gm15270 NNNCAATGATAACTTTGTCA NGG 2 0.2588 Tier III
98 NC_000079.6 13 78433832 - Gm31946 NNNCAATGATAACTTTGTCA NGG 2 0.2588 Tier III
99 NC_000073.6 7 61763035 + A230057D06Rik NNNAAATGATAACTTGGTCA NGG 2 0.2538 Tier III
100 NC_000075.6 9 17313979 + Gm39296 NNNAAATGATAACTTGGTCA NGG 2 0.2538 Tier III
101 NC_000079.6 13 81000434 + 9330111N05Rik NNNAAATGATAACTTGGTCA NGG 2 0.2538 Tier III
102 NC_000084.6 18 5530428 + Gm10125 NNNAAATGATAACTTGGTCA NGG 2 0.2538 Tier III
103 NC_000070.6 4 89495441 - Gm12610 NNNGAATAATAACTTGGTCA NGG 2 0.2163 Tier III
104 NC_000073.6 7 61545019 + B230209E15Rik NNNAAATAATAATTTTGTCA NAG 2 0.1815 Tier III
105 NC_000077.6 11 40181314 + Gm12132 NNNAATTAATAACTTTTTCA NGG 2 0.1786 Tier III
106 NC_000070.6 4 4384701 - Gm33007 NNNAAATAACATCTTTGTCA NGG 2 0.1778 Tier III
107 NC_000070.6 4 80717112 - Gm46885 NNNGAATAATAACTTTGTCA NAG 2 0.162 Tier III
108 NC_000072.6 6 102272271 + 4930587E11Rik NNNAAATATTAACTTTGTCA NAG 2 0.1556 Tier III
109 NC_000067.6 1 20385478 + 4930486I03Rik NNNCAATAATAACTTGGTCA NGG 2 0.1222 Tier III
110 NC_000067.6 1 79173878 + Gm29536 NNNCAATAATAACTTGGTCA NGG 2 0.1222 Tier III
111 NC_000068.7 2 105500008 - 4930527A07Rik NNNCAATAATAACTTGGTCA NGG 2 0.1222 Tier III
112 NC_000068.7 2 177361184 - Gm14412 NNNCAATAATAACTTGGTCA NGG 2 0.1222 Tier III
113 NC_000069.6 3 58098044 - Gm40056 NNNCAATAATAACTTGGTCA NGG 2 0.1222 Tier III
114 NC_000071.6 5 39552397 - 4930513D17Rik NNNCAATAATAACTTGGTCA NGG 2 0.1222 Tier III
115 NC_000072.6 6 66178354 - Gm36408 NNNCAATAATAACTTGGTCA NGG 2 0.1222 Tier III
116 NC_000077.6 11 55839758 - LOC105244170 NNNCAATAATAACTTGGTCA NGG 2 0.1222 Tier III
117 NC_000083.6 17 9383161 + Gm7947 NNNCAATAATAACTTGGTCA NGG 2 0.1222 Tier III
118 NC_000083.6 17 90487679 + Gm32337 NNNCAATAATAACTTGGTCA NGG 2 0.1222 Tier III
119 NC_000074.6 8 41887264 + 2810404M03Rik NNNAAATAATAACCTGGTCA NGG 2 0.0989 Tier III
120 NC_000067.6 1 157503844 - Gm15486 NNNAAATAATAACTTGTTCA NGG 2 0.0865 Tier III
121 NC_000067.6 1 79052504 - Gm29536 NNNAAATAATAACTTGCTCA NGG 2 0.0814 Tier III
122 NC_000079.6 13 43257751 + LOC115488162 NNNACATAATAACTTTGTCA NGA 2 0.0347 Tier III
123 NC_000073.6 7 136289950 + C030029H02Rik NNNAAATAATAAATTTGTCA NGA 2 0.0267 Tier III
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Other clones with same target sequence:

(none)