Construct: sgRNA BRDN0001146383
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AGGAAATAATAACTTTGTCA
Original Target:
- Taxon:
- Homo sapiens (human)
- Gene:
- GK (2710)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
Reference Sequence | Chromosome | Cut Position | Match Strand | Match Gene | Match Sequence | PAM Seq. | Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. | CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. | Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. | |
---|---|---|---|---|---|---|---|---|---|---|
1 | NC_000023.11 | X | 30691195 | + | GK | NNNAAATAATAACTTTGTCA | NGG | 0 | 1.0 | Tier I |
2 | NC_000003.12 | 3 | 57277900 | - | ASB14 | NNNAAGTAATAACTTTGTCA | NGG | 1 | 0.7143 | Tier I |
3 | NC_000004.12 | 4 | 165279237 | - | GK3 | NNNAAATAATAACTTTGTCA | NGT | 1 | 0.0161 | Tier I |
4 | NC_000015.10 | 15 | 73057580 | + | NEO1 | NNNAAAAATTAACTTTGTCA | NGG | 2 | 0.525 | Tier II |
5 | NC_000002.12 | 2 | 143035074 | - | KYNU | NNNAAATAAAAACTTTGTCT | NGG | 2 | 0.5143 | Tier II |
6 | NC_000001.11 | 1 | 97779149 | + | DPYD | NNNAAATAATAATTTTGACA | NGG | 2 | 0.4667 | Tier II |
7 | NC_000014.9 | 14 | 52646823 | + | ERO1A | NNNACAAAATAACTTTGTCA | NGG | 2 | 0.4375 | Tier II |
8 | NC_000003.12 | 3 | 173734802 | - | NLGN1 | NNNAGATATTAACTTTGTCA | NGG | 2 | 0.432 | Tier II |
9 | NC_000010.11 | 10 | 115549350 | - | ATRNL1 | NNNAAGTAATAACTTTGTCT | NGG | 2 | 0.4286 | Tier II |
10 | NC_000002.12 | 2 | 182614196 | + | PDE1A | NNNAACTAATAACTTAGTCA | NGG | 2 | 0.4132 | Tier II |
11 | NC_000011.10 | 11 | 21406100 | + | NELL1 | NNNAAATAAAAACTTTGTAA | NGG | 2 | 0.3673 | Tier II |
12 | NC_000004.12 | 4 | 22439844 | + | ADGRA3 | NNNACATAGTAACTTTGTCA | NGG | 2 | 0.3333 | Tier II |
13 | NC_000012.12 | 12 | 43926742 | + | TMEM117 | NNNAAATAATATCTTTATCA | NGG | 2 | 0.3111 | Tier II |
14 | NC_000001.11 | 1 | 233661189 | - | KCNK1 | NNNAAATAATAACTTAGGCA | NGG | 2 | 0.303 | Tier II |
15 | NC_000006.12 | 6 | 163019206 | + | PACRG | NNNAAATAATAATTTTGTAA | NGG | 2 | 0.3 | Tier II |
16 | NC_000003.12 | 3 | 55005831 | - | CACNA2D3 | NNNAAATAATAACATTGTAA | NGG | 2 | 0.2653 | Tier II |
17 | NC_000007.14 | 7 | 702038 | + | PRKAR1B | NNNAAATTATAACATTGTCA | NGG | 2 | 0.2653 | Tier II |
18 | NC_000005.10 | 5 | 172434741 | - | SH3PXD2B | NNNATGTAATAACTTTGTCA | NGG | 2 | 0.2597 | Tier II |
19 | NC_000012.12 | 12 | 92796719 | + | EEA1 | NNNCAATGATAACTTTGTCA | NGG | 2 | 0.2588 | Tier II |
20 | NC_000002.12 | 2 | 149024079 | - | KIF5C | NNNAAATAATAACTTATTCA | NGG | 2 | 0.2273 | Tier II |
21 | NC_000013.11 | 13 | 93997441 | + | GPC6 | NNNAAATAATGACTTGGTCA | NGG | 2 | 0.225 | Tier II |
22 | NC_000008.11 | 8 | 138637696 | + | COL22A1 | NNNAAATAGTATCTTTGTCA | NGG | 2 | 0.2222 | Tier II |
23 | NC_000013.11 | 13 | 26379399 | - | CDK8 | NNNAAATAGTATCTTTGTCA | NGG | 2 | 0.2222 | Tier II |
24 | NC_000003.12 | 3 | 154383591 | - | GPR149 | NNNAAATCAGAACTTTGTCA | NGG | 2 | 0.2143 | Tier II |
25 | NC_000001.11 | 1 | 26443924 | - | DHDDS | NNNTAATAATATCTTTGTCA | NGG | 2 | 0.2121 | Tier II |
26 | NC_000003.12 | 3 | 69258837 | - | FRMD4B | NNNAAAAAATAACTTTCTCA | NGG | 2 | 0.2059 | Tier II |
27 | NC_000006.12 | 6 | 118002672 | + | SLC35F1 | NNNAAATAATAATTTTTTCA | NGG | 2 | 0.175 | Tier II |
28 | NC_000002.12 | 2 | 222220849 | + | PAX3 | NNNAAATAATGACTTTGTCA | NAG | 2 | 0.1685 | Tier II |
29 | NC_000014.9 | 14 | 91109166 | - | DGLUCY | NNNAAATAATACCATTGTCA | NGG | 2 | 0.1629 | Tier II |
30 | NC_000008.11 | 8 | 42482292 | - | SLC20A2 | NNNTAATAATAACTTTTTCA | NGG | 2 | 0.1591 | Tier II |
31 | NC_000006.12 | 6 | 159222842 | - | FNDC1 | NNNAAATAATAACATTTTCA | NGG | 2 | 0.1548 | Tier II |
32 | NC_000004.12 | 4 | 86992657 | - | AFF1 | NNNCAATTATAACTTTGTCA | NGG | 2 | 0.1513 | Tier II |
33 | NC_000004.12 | 4 | 155185880 | + | NPY2R | NNNAAATATTAACTTTTTCA | NGG | 2 | 0.15 | Tier II |
34 | NC_000002.12 | 2 | 27868998 | - | RBKS | NNNTAATAATAACTTTCTCA | NGG | 2 | 0.1497 | Tier II |
35 | NC_000010.11 | 10 | 85658387 | - | GRID1 | NNNAAATAATAACTTTATGA | NGG | 2 | 0.1167 | Tier II |
36 | NC_000007.14 | 7 | 95057030 | - | PPP1R9A | NNNAAATAATACATTTGTCA | NGG | 2 | 0.1012 | Tier II |
37 | NC_000009.12 | 9 | 10401285 | + | PTPRD | NNNAAATAATAAATTTTTCA | NGG | 2 | 0.0962 | Tier II |
38 | NC_000003.12 | 3 | 88114479 | + | CGGBP1 | NNNAAATAATAAATTTCTCA | NGG | 2 | 0.0905 | Tier II |
39 | NC_000003.12 | 3 | 88114479 | + | ZNF654 | NNNAAATAATAAATTTCTCA | NGG | 2 | 0.0905 | Tier II |
40 | NC_000001.11 | 1 | 156731423 | - | METTL25B | NNNAAATAATAACTTTGGCC | NGG | 2 | 0.0758 | Tier II |
41 | NC_000002.12 | 2 | 63176082 | - | WDPCP | NNNAAATAACAAGTTTGTCA | NGG | 2 | 0.0727 | Tier II |
42 | NC_000009.12 | 9 | 39259016 | + | CNTNAP3 | NNNAAATACTAACTTTGTGA | NGG | 2 | 0.0714 | Tier II |
43 | NC_000009.12 | 9 | 42096876 | + | CNTNAP3B | NNNAAATACTAACTTTGTGA | NGG | 2 | 0.0714 | Tier II |
44 | NC_000009.12 | 9 | 61360019 | - | CNTNAP3C | NNNAAATACTAACTTTGTGA | NGG | 2 | 0.0714 | Tier II |
45 | NC_000003.12 | 3 | 165064121 | + | SI | NNNAAATAATAACTTAGTCA | NGA | 2 | 0.0631 | Tier II |
46 | NC_000011.10 | 11 | 101564432 | - | TRPC6 | NNNAAATAATAAGTTTGTAA | NGG | 2 | 0.0584 | Tier II |
47 | NC_000002.12 | 2 | 157313796 | - | GALNT5 | NNNATATAATAAGTTTGTCA | NGG | 2 | 0.0496 | Tier II |
48 | NC_000005.10 | 5 | 11119673 | + | CTNND2 | NNNAAATAATAAGTTTGTCA | NAG | 2 | 0.0354 | Tier II |
49 | NC_000001.11 | 1 | 243592789 | + | AKT3 | NNNAAATAGTAACTGTGTCA | NGG | 2 | 0.0333 | Tier II |
50 | NC_000003.12 | 3 | 17686383 | + | TBC1D5 | NNNAAATAATAACTTTGTGA | NAG | 2 | 0.0324 | Tier II |
51 | NC_000008.11 | 8 | 119218042 | + | MAL2 | NNNGAATAATAACTGTGTCA | NGG | 2 | 0.0313 | Tier II |
52 | NC_000007.14 | 7 | 121370474 | - | FAM3C | NNNAAATAATAAATTTGTCA | NGA | 2 | 0.0267 | Tier II |
53 | NC_000011.10 | 11 | 96135196 | + | MAML2 | NNNAAATAATAACTTTGTCT | NTG | 2 | 0.0234 | Tier II |
54 | NC_000001.11 | 1 | 215945441 | + | USH2A | NNNAAATAATATCTTTGTCA | NGA | 2 | 0.0231 | Tier II |
55 | NC_000005.10 | 5 | 37551433 | - | WDR70 | NNNAAATAATAACTTTTTCA | NGA | 2 | 0.0174 | Tier II |
56 | NC_000007.14 | 7 | 90886772 | - | CDK14 | NNNAAATAATAACTTTCTCA | NGA | 2 | 0.0163 | Tier II |
57 | NC_000004.12 | 4 | 165279237 | - | KLHL2 | NNNAAATAATAACTTTGTCA | NGT | 1 | 0.0161 | Tier II |
58 | NC_000017.11 | 17 | 1390268 | - | YWHAE | NNNAAATAATAATTTTGTCA | NGT | 2 | 0.0113 | Tier II |
59 | NC_000017.11 | 17 | 60397225 | + | USP32 | NNNTAATAATAACTTTGTCA | NGT | 2 | 0.0103 | Tier II |
60 | NC_000007.14 | 7 | 32304871 | - | PDE1C | NNNAAATAATAACATTGTCA | NGT | 2 | 0.01 | Tier II |
61 | NC_000004.12 | 4 | 161784567 | - | FSTL5 | NNNAAATAATAACTTTCTCA | NTG | 2 | 0.0092 | Tier II |
62 | NC_000003.12 | 3 | 121028288 | + | STXBP5L | NNNAAATAATAACTTTTTCA | NGC | 2 | 0.0056 | Tier II |
63 | NC_000018.10 | 18 | 61370601 | - | CDH20 | NNNAAATAATAACTTTGTGA | NGT | 2 | 0.002 | Tier II |
64 | NC_000003.12 | 3 | 57277900 | - | LOC105377102 | NNNAAGTAATAACTTTGTCA | NGG | 1 | 0.7143 | Tier III |
65 | NC_000003.12 | 3 | 173734802 | - | LOC105374225 | NNNAGATATTAACTTTGTCA | NGG | 2 | 0.432 | Tier III |
66 | NC_000005.10 | 5 | 128708465 | - | LOC105379168 | NNNAAATAATAACTTTGTTG | NGG | 2 | 0.3529 | Tier III |
67 | NC_000023.11 | X | 33821926 | + | LOC105373153 | NNNAAAAAATAACTTTGTCA | NAG | 2 | 0.2269 | Tier III |
68 | NC_000003.12 | 3 | 103993704 | + | LOC124909491 | NNNAAATAGTAACTCTGTCA | NGG | 2 | 0.1818 | Tier III |
69 | NC_000012.12 | 12 | 68684370 | - | LOC100507250 | NNNAAACAATACCTTTGTCA | NGG | 2 | 0.1809 | Tier III |
70 | NC_000002.12 | 2 | 222220849 | + | LOC107985991 | NNNAAATAATGACTTTGTCA | NAG | 2 | 0.1685 | Tier III |
71 | NC_000009.12 | 9 | 41427825 | + | LOC107984035 | NNNAAATAATAACTTTTACA | NGG | 2 | 0.1667 | Tier III |
72 | NC_000009.12 | 9 | 41427825 | + | LOC124900272 | NNNAAATAATAACTTTTACA | NGG | 2 | 0.1667 | Tier III |
73 | NC_000002.12 | 2 | 212951069 | - | LOC102725082 | NNNAACTAATATCTTTGTCA | NGG | 2 | 0.1515 | Tier III |
74 | NC_000012.12 | 12 | 58720278 | - | LOC100506869 | NNNAAATAATAAATTTGGCA | NGG | 2 | 0.1282 | Tier III |
75 | NC_000012.12 | 12 | 58720278 | - | LINC02388 | NNNAAATAATAAATTTGGCA | NGG | 2 | 0.1282 | Tier III |
76 | NC_000018.10 | 18 | 14636812 | + | LOC105372004 | NNNAAATAATAAATTTGGCA | NGG | 2 | 0.1282 | Tier III |
77 | NC_000005.10 | 5 | 114560399 | - | LOC101927078 | NNNAAATAATAACTTAGTGA | NGG | 2 | 0.1136 | Tier III |
78 | NC_000002.12 | 2 | 56043208 | + | MIR217HG | NNNAAATAATAACTTTCTTA | NGG | 2 | 0.1086 | Tier III |
79 | NC_000002.12 | 2 | 56043208 | + | LOC105374690 | NNNAAATAATAACTTTCTTA | NGG | 2 | 0.1086 | Tier III |
80 | NC_000007.14 | 7 | 95057030 | - | PPP1R9A-AS1 | NNNAAATAATACATTTGTCA | NGG | 2 | 0.1012 | Tier III |
81 | NC_000001.11 | 1 | 71176519 | - | ZRANB2-DT | NNNAAATAATAAATTTCTCA | NGG | 2 | 0.0905 | Tier III |
82 | NC_000009.12 | 9 | 67090567 | - | CNTNAP3P2 | NNNAAATACTAACTTTGTGA | NGG | 2 | 0.0714 | Tier III |
83 | NC_000008.11 | 8 | 119218042 | + | MAL2-AS1 | NNNGAATAATAACTGTGTCA | NGG | 2 | 0.0313 | Tier III |
84 | NC_000015.10 | 15 | 36468176 | - | LOC105370768 | NNNAAATAATAATTTTGTCA | NTG | 2 | 0.0273 | Tier III |
85 | NC_000021.9 | 21 | 16210263 | + | MIR99AHG | NNNAAATAATAAGTTTGTGA | NGG | 2 | 0.017 | Tier III |
86 | NC_000004.12 | 4 | 119394549 | - | GK6P | NNNAAATAATAACTTTGTCA | NGT | 1 | 0.0161 | Tier III |
87 | NC_000004.12 | 4 | 119394549 | - | LOC107986193 | NNNAAATAATAACTTTGTCA | NGT | 1 | 0.0161 | Tier III |
88 | NC_000023.11 | X | 101780132 | - | GK4P | NNNAAATAATAACTTTGTCA | NGT | 1 | 0.0161 | Tier III |
89 | NC_000007.14 | 7 | 13719929 | - | LOC105375161 | NNNAAATAATTACTTTGTCA | NTG | 2 | 0.012 | Tier III |
90 | NC_000021.9 | 21 | 10050919 | - | LOC105372733 | NNNAAATAATAACTTTTTCA | NTG | 2 | 0.0097 | Tier III |
91 | NT_187374.1 | 9 | 20976 | - | LOC107987370 | NNNAAATAATAACTTTTTCA | NTG | 2 | 0.0097 | Tier III |
92 | NC_000017.11 | 17 | 62814668 | - | MARCHF10-DT | NNNAAATAATAACTTGGTCA | NGT | 2 | 0.0056 | Tier III |
93 | NC_000003.12 | 3 | 34914795 | + | LOC101928135 | NNNAAATAATAACTTTGTCA | NTC | 2 | 0.0 | Tier III |
94 | NC_000008.11 | 8 | 95684825 | - | CFAP418-AS1 | NNNAAATAATAACTTTGTCA | NAC | 2 | 0.0 | Tier III |
95 | NC_000014.9 | 14 | 86118911 | - | LINC02328 | NNNAAATAATAACTTTGTCA | NCT | 2 | 0.0 | Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
Reference Sequence | Chromosome | Cut Position | Match Strand | Match Gene | Match Sequence | PAM Seq. | Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. | CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. | Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. | |
---|---|---|---|---|---|---|---|---|---|---|
1 | NC_000086.7 | X | 85746230 | - | Gk | NNNAAATAATAACTTTGTCA | NGG | 0 | 1.0 | Tier I |
2 | NC_000080.6 | 14 | 26914198 | + | Asb14 | NNNAAGTCATAACTTTGTCA | NGG | 2 | 0.3061 | Tier I |
3 | NC_000074.6 | 8 | 93119156 | - | Ces1c | NNNAAATATTAACTTTGTCA | NGG | 1 | 0.6 | Tier II |
4 | NC_000067.6 | 1 | 123621454 | + | Dpp10 | NNNAAATAATAACATAGTCA | NGG | 2 | 0.5628 | Tier II |
5 | NC_000080.6 | 14 | 57534016 | + | Il17d | NNNAAATAAAAACATTGTCA | NGG | 2 | 0.5306 | Tier II |
6 | NC_000073.6 | 7 | 133828838 | + | Fank1 | NNNAAATAGTAGCTTTGTCA | NGG | 2 | 0.4815 | Tier II |
7 | NC_000067.6 | 1 | 60754758 | + | Cd28 | NNNAATTAATGACTTTGTCA | NGG | 2 | 0.4643 | Tier II |
8 | NC_000072.6 | 6 | 61286157 | - | Ccser1 | NNNAACTAATAACTTTGTCA | NGG | 1 | 0.4545 | Tier II |
9 | NC_000067.6 | 1 | 24061681 | - | Fam135a | NNNAAACAATAACTTTGTCT | NGG | 2 | 0.4125 | Tier II |
10 | NC_000085.6 | 19 | 58792899 | + | 1700019N19Rik | NNNAAAGAATGACTTTGTCA | NGG | 2 | 0.3824 | Tier II |
11 | NC_000085.6 | 19 | 37582449 | + | Exoc6 | NNNAAATAATCACTTTATCA | NGG | 2 | 0.3733 | Tier II |
12 | NC_000068.7 | 2 | 40760489 | - | Lrp1b | NNNAAATAATAAATTTATCA | NGG | 2 | 0.359 | Tier II |
13 | NC_000071.6 | 5 | 7011378 | + | Zfp804b | NNNCAATAATAACTTTGTCA | NGG | 1 | 0.3529 | Tier II |
14 | NC_000086.7 | X | 161233202 | - | Scml2 | NNNAAATAATAAATTAGTCA | NGG | 2 | 0.3497 | Tier II |
15 | NC_000086.7 | X | 50874864 | - | Stk26 | NNNAAAGACTAACTTTGTCA | NGG | 2 | 0.3361 | Tier II |
16 | NC_000076.6 | 10 | 4958669 | - | Esr1 | NNNAAATAATATCTTTGTCA | NGG | 1 | 0.3333 | Tier II |
17 | NC_000073.6 | 7 | 126887221 | + | Tmem219 | NNNAAATAATAATTTTGCCA | NGG | 2 | 0.3 | Tier II |
18 | NC_000067.6 | 1 | 124255211 | + | Dpp10 | NNNCAATGATAACTTTGTCA | NGG | 2 | 0.2588 | Tier II |
19 | NC_000068.7 | 2 | 16062181 | + | Malrd1 | NNNCAATGATAACTTTGTCA | NGG | 2 | 0.2588 | Tier II |
20 | NC_000069.6 | 3 | 15382879 | + | Sirpb1a | NNNCAATGATAACTTTGTCA | NGG | 2 | 0.2588 | Tier II |
21 | NC_000069.6 | 3 | 15506883 | + | Sirpb1b | NNNCAATGATAACTTTGTCA | NGG | 2 | 0.2588 | Tier II |
22 | NC_000069.6 | 3 | 15806261 | + | Sirpb1c | NNNCAATGATAACTTTGTCA | NGG | 2 | 0.2588 | Tier II |
23 | NC_000072.6 | 6 | 8387600 | - | Umad1 | NNNCAATGATAACTTTGTCA | NGG | 2 | 0.2588 | Tier II |
24 | NC_000072.6 | 6 | 64185232 | - | Grid2 | NNNCAATGATAACTTTGTCA | NGG | 2 | 0.2588 | Tier II |
25 | NC_000073.6 | 7 | 7224607 | + | Vmn2r29 | NNNCAATGATAACTTTGTCA | NGG | 2 | 0.2588 | Tier II |
26 | NC_000077.6 | 11 | 9281827 | - | Abca13 | NNNCAATGATAACTTTGTCA | NGG | 2 | 0.2588 | Tier II |
27 | NC_000079.6 | 13 | 14301750 | - | Hecw1 | NNNCAATGATAACTTTGTCA | NGG | 2 | 0.2588 | Tier II |
28 | NC_000079.6 | 13 | 104326275 | - | Adamts6 | NNNCAATGATAACTTTGTCA | NGG | 2 | 0.2588 | Tier II |
29 | NC_000080.6 | 14 | 30741573 | - | Sfmbt1 | NNNCAATGATAACTTTGTCA | NGG | 2 | 0.2588 | Tier II |
30 | NC_000082.6 | 16 | 16015225 | - | Spidr | NNNCAATGATAACTTTGTCA | NGG | 2 | 0.2588 | Tier II |
31 | NC_000084.6 | 18 | 14929803 | - | Taf4b | NNNCAATGATAACTTTGTCA | NGG | 2 | 0.2588 | Tier II |
32 | NC_000084.6 | 18 | 31166509 | - | Rit2 | NNNCAATGATAACTTTGTCA | NGG | 2 | 0.2588 | Tier II |
33 | NC_000085.6 | 19 | 32081660 | + | Asah2 | NNNCAATGATAACTTTGTCA | NGG | 2 | 0.2588 | Tier II |
34 | NC_000086.7 | X | 141604818 | + | Col4a5 | NNNCAATGATAACTTTGTCA | NGG | 2 | 0.2588 | Tier II |
35 | NC_000086.7 | X | 144978223 | + | Rtl4 | NNNCAATGATAACTTTGTCA | NGG | 2 | 0.2588 | Tier II |
36 | NC_000067.6 | 1 | 21516397 | + | Kcnq5 | NNNAAATGATAACTTGGTCA | NGG | 2 | 0.2538 | Tier II |
37 | NC_000068.7 | 2 | 15911011 | - | Malrd1 | NNNAAATGATAACTTGGTCA | NGG | 2 | 0.2538 | Tier II |
38 | NC_000068.7 | 2 | 112580248 | - | Aven | NNNAAATGATAACTTGGTCA | NGG | 2 | 0.2538 | Tier II |
39 | NC_000069.6 | 3 | 119268270 | - | Dpyd | NNNAAATGATAACTTGGTCA | NGG | 2 | 0.2538 | Tier II |
40 | NC_000072.6 | 6 | 84994451 | - | Exoc6b | NNNAAATGATAACTTGGTCA | NGG | 2 | 0.2538 | Tier II |
41 | NC_000076.6 | 10 | 83271567 | - | Slc41a2 | NNNAAATGATAACTTGGTCA | NGG | 2 | 0.2538 | Tier II |
42 | NC_000079.6 | 13 | 10226745 | + | Chrm3 | NNNAAATGATAACTTGGTCA | NGG | 2 | 0.2538 | Tier II |
43 | NC_000079.6 | 13 | 104439542 | - | Adamts6 | NNNAAATGATAACTTGGTCA | NGG | 2 | 0.2538 | Tier II |
44 | NC_000086.7 | X | 101094627 | - | Snx12 | NNNAAATGATAACTTGGTCA | NGG | 2 | 0.2538 | Tier II |
45 | NC_000079.6 | 13 | 36102382 | - | Lyrm4 | NNNAAATAATAATTTGGTCA | NGG | 2 | 0.2423 | Tier II |
46 | NC_000076.6 | 10 | 33119420 | - | Trdn | NNNCAATAATAACTTCGTCA | NGG | 2 | 0.2353 | Tier II |
47 | NC_000073.6 | 7 | 17914547 | - | Gm5155 | NNNTAATAATAACTTGGTCA | NGG | 2 | 0.2203 | Tier II |
48 | NC_000086.7 | X | 138671356 | - | Il1rapl2 | NNNTAATAATAACTTGGTCA | NGG | 2 | 0.2203 | Tier II |
49 | NC_000072.6 | 6 | 106789503 | - | Crbn | NNNAAATGATAACTCTGTCA | NGG | 2 | 0.2 | Tier II |
50 | NC_000084.6 | 18 | 53926407 | + | Csnk1g3 | NNNTAATAATTACTTTGTCA | NGG | 2 | 0.1958 | Tier II |
51 | NC_000080.6 | 14 | 17765546 | - | Thrb | NNNAAATGATACCTTTGTCA | NGG | 2 | 0.193 | Tier II |
52 | NC_000071.6 | 5 | 134720908 | + | Eln | NNNAAATAATGACTCTGTCA | NGG | 2 | 0.1773 | Tier II |
53 | NC_000074.6 | 8 | 85374015 | - | Mylk3 | NNNAAATACTTACTTTGTCA | NGG | 2 | 0.1758 | Tier II |
54 | NC_000081.6 | 15 | 58074800 | + | Zhx1 | NNNAAATAATGACTTTTTCA | NGG | 2 | 0.1625 | Tier II |
55 | NC_000072.6 | 6 | 102272271 | + | Cntn3 | NNNAAATATTAACTTTGTCA | NAG | 2 | 0.1556 | Tier II |
56 | NC_000076.6 | 10 | 115579235 | + | Lgr5 | NNNAAATAATATCTTTGTTA | NGG | 2 | 0.1538 | Tier II |
57 | NC_000076.6 | 10 | 115579235 | + | A930009A15Rik | NNNAAATAATATCTTTGTTA | NGG | 2 | 0.1538 | Tier II |
58 | NC_000080.6 | 14 | 59958460 | - | Atp8a2 | NNNAAAGAATAACTTTCTCA | NGG | 2 | 0.1384 | Tier II |
59 | NC_000067.6 | 1 | 6515253 | - | St18 | NNNCAATAATAACTTGGTCA | NGG | 2 | 0.1222 | Tier II |
60 | NC_000067.6 | 1 | 20385478 | + | Pkhd1 | NNNCAATAATAACTTGGTCA | NGG | 2 | 0.1222 | Tier II |
61 | NC_000067.6 | 1 | 70332339 | + | Spag16 | NNNCAATAATAACTTGGTCA | NGG | 2 | 0.1222 | Tier II |
62 | NC_000068.7 | 2 | 77537530 | - | Zfp385b | NNNCAATAATAACTTGGTCA | NGG | 2 | 0.1222 | Tier II |
63 | NC_000068.7 | 2 | 137053100 | - | Slx4ip | NNNCAATAATAACTTGGTCA | NGG | 2 | 0.1222 | Tier II |
64 | NC_000069.6 | 3 | 84121715 | - | Mnd1 | NNNCAATAATAACTTGGTCA | NGG | 2 | 0.1222 | Tier II |
65 | NC_000070.6 | 4 | 14910455 | - | Pip4p2 | NNNCAATAATAACTTGGTCA | NGG | 2 | 0.1222 | Tier II |
66 | NC_000071.6 | 5 | 17668507 | - | Sema3c | NNNCAATAATAACTTGGTCA | NGG | 2 | 0.1222 | Tier II |
67 | NC_000071.6 | 5 | 109431041 | - | Vmn2r17 | NNNCAATAATAACTTGGTCA | NGG | 2 | 0.1222 | Tier II |
68 | NC_000073.6 | 7 | 107152215 | + | Nlrp14 | NNNCAATAATAACTTGGTCA | NGG | 2 | 0.1222 | Tier II |
69 | NC_000074.6 | 8 | 68304171 | - | Sh2d4a | NNNCAATAATAACTTGGTCA | NGG | 2 | 0.1222 | Tier II |
70 | NC_000076.6 | 10 | 33205672 | - | Trdn | NNNCAATAATAACTTGGTCA | NGG | 2 | 0.1222 | Tier II |
71 | NC_000077.6 | 11 | 29940413 | + | Eml6 | NNNCAATAATAACTTGGTCA | NGG | 2 | 0.1222 | Tier II |
72 | NC_000077.6 | 11 | 105784118 | + | Tanc2 | NNNCAATAATAACTTGGTCA | NGG | 2 | 0.1222 | Tier II |
73 | NC_000078.6 | 12 | 37925656 | - | Dgkb | NNNCAATAATAACTTGGTCA | NGG | 2 | 0.1222 | Tier II |
74 | NC_000081.6 | 15 | 21525464 | - | Cdh12 | NNNCAATAATAACTTGGTCA | NGG | 2 | 0.1222 | Tier II |
75 | NC_000082.6 | 16 | 11732933 | - | Snx29 | NNNCAATAATAACTTGGTCA | NGG | 2 | 0.1222 | Tier II |
76 | NC_000083.6 | 17 | 90487679 | + | Nrxn1 | NNNCAATAATAACTTGGTCA | NGG | 2 | 0.1222 | Tier II |
77 | NC_000086.7 | X | 43064631 | + | Tenm1 | NNNCAATAATAACTTGGTCA | NGG | 2 | 0.1222 | Tier II |
78 | NC_000074.6 | 8 | 49317753 | - | Tenm3 | NNNAAATAAAAAGTTTGTCA | NGG | 2 | 0.1169 | Tier II |
79 | NC_000083.6 | 17 | 20354144 | - | Vmn2r107 | NNNAAATAATAACTTTGTAA | NAG | 2 | 0.1111 | Tier II |
80 | NC_000074.6 | 8 | 90255120 | + | Tox3 | NNNAAATAATAACTTTCTAA | NGG | 2 | 0.1008 | Tier II |
81 | NC_000079.6 | 13 | 93840555 | + | Arsb | NNNAAATAATTACCTTGTCA | NGG | 2 | 0.0879 | Tier II |
82 | NC_000078.6 | 12 | 75357796 | + | Rhoj | NNNAAATAATAACTTGTTCA | NGG | 2 | 0.0865 | Tier II |
83 | NC_000075.6 | 9 | 58626424 | + | Nptn | NNNAAATAATACCTTTTTCA | NGG | 2 | 0.0658 | Tier II |
84 | NC_000067.6 | 1 | 50934362 | - | Tmeff2 | NNNAAATAATAACTTTTTCA | NAG | 2 | 0.0648 | Tier II |
85 | NC_000078.6 | 12 | 112009405 | - | Tdrd9 | NNNAAATAATAACTTTTTCA | NAG | 2 | 0.0648 | Tier II |
86 | NC_000079.6 | 13 | 43257751 | + | Gfod1 | NNNACATAATAACTTTGTCA | NGA | 2 | 0.0347 | Tier II |
87 | NC_000072.6 | 6 | 40447641 | + | Wee2 | NNNAAACAATAACTTTGTCA | NTG | 2 | 0.0268 | Tier II |
88 | NC_000078.6 | 12 | 61608646 | - | Lrfn5 | NNNAAATTATAACTTTGTCA | NTG | 2 | 0.0167 | Tier II |
89 | NC_000082.6 | 16 | 67499307 | + | Cadm2 | NNNAAATAATAACATTGTCA | NGT | 2 | 0.01 | Tier II |
90 | NC_000082.6 | 16 | 97893993 | + | C2cd2 | NNNAAATAATTACTTTGTCA | NGC | 2 | 0.0068 | Tier II |
91 | NC_000086.7 | X | 35776018 | - | Gm39502 | NNNAAATGATAACTTAGTCA | NGG | 2 | 0.6667 | Tier III |
92 | NC_000073.6 | 7 | 120842203 | - | Gm36449 | NNNAATTAATAACTTTATCA | NGG | 2 | 0.6667 | Tier III |
93 | NC_000070.6 | 4 | 24003371 | - | Gm27243 | NNNAAATAATAATTTTATCA | NGG | 2 | 0.6533 | Tier III |
94 | NC_000069.6 | 3 | 126173742 | - | Gm40146 | NNNTAATGATAACTTTGTCA | NGG | 2 | 0.4667 | Tier III |
95 | NC_000067.6 | 1 | 33324628 | + | Gm39617 | NNNAAATACTAACTTTGACA | NGG | 2 | 0.381 | Tier III |
96 | NC_000072.6 | 6 | 141905286 | + | Gm30843 | NNNCAATGATAACTTTGTCA | NGG | 2 | 0.2588 | Tier III |
97 | NC_000076.6 | 10 | 24428392 | + | Gm15270 | NNNCAATGATAACTTTGTCA | NGG | 2 | 0.2588 | Tier III |
98 | NC_000079.6 | 13 | 78433832 | - | Gm31946 | NNNCAATGATAACTTTGTCA | NGG | 2 | 0.2588 | Tier III |
99 | NC_000073.6 | 7 | 61763035 | + | A230057D06Rik | NNNAAATGATAACTTGGTCA | NGG | 2 | 0.2538 | Tier III |
100 | NC_000075.6 | 9 | 17313979 | + | Gm39296 | NNNAAATGATAACTTGGTCA | NGG | 2 | 0.2538 | Tier III |
101 | NC_000079.6 | 13 | 81000434 | + | 9330111N05Rik | NNNAAATGATAACTTGGTCA | NGG | 2 | 0.2538 | Tier III |
102 | NC_000084.6 | 18 | 5530428 | + | Gm10125 | NNNAAATGATAACTTGGTCA | NGG | 2 | 0.2538 | Tier III |
103 | NC_000070.6 | 4 | 89495441 | - | Gm12610 | NNNGAATAATAACTTGGTCA | NGG | 2 | 0.2163 | Tier III |
104 | NC_000073.6 | 7 | 61545019 | + | B230209E15Rik | NNNAAATAATAATTTTGTCA | NAG | 2 | 0.1815 | Tier III |
105 | NC_000077.6 | 11 | 40181314 | + | Gm12132 | NNNAATTAATAACTTTTTCA | NGG | 2 | 0.1786 | Tier III |
106 | NC_000070.6 | 4 | 4384701 | - | Gm33007 | NNNAAATAACATCTTTGTCA | NGG | 2 | 0.1778 | Tier III |
107 | NC_000070.6 | 4 | 80717112 | - | Gm46885 | NNNGAATAATAACTTTGTCA | NAG | 2 | 0.162 | Tier III |
108 | NC_000072.6 | 6 | 102272271 | + | 4930587E11Rik | NNNAAATATTAACTTTGTCA | NAG | 2 | 0.1556 | Tier III |
109 | NC_000067.6 | 1 | 20385478 | + | 4930486I03Rik | NNNCAATAATAACTTGGTCA | NGG | 2 | 0.1222 | Tier III |
110 | NC_000067.6 | 1 | 79173878 | + | Gm29536 | NNNCAATAATAACTTGGTCA | NGG | 2 | 0.1222 | Tier III |
111 | NC_000068.7 | 2 | 105500008 | - | 4930527A07Rik | NNNCAATAATAACTTGGTCA | NGG | 2 | 0.1222 | Tier III |
112 | NC_000068.7 | 2 | 177361184 | - | Gm14412 | NNNCAATAATAACTTGGTCA | NGG | 2 | 0.1222 | Tier III |
113 | NC_000069.6 | 3 | 58098044 | - | Gm40056 | NNNCAATAATAACTTGGTCA | NGG | 2 | 0.1222 | Tier III |
114 | NC_000071.6 | 5 | 39552397 | - | 4930513D17Rik | NNNCAATAATAACTTGGTCA | NGG | 2 | 0.1222 | Tier III |
115 | NC_000072.6 | 6 | 66178354 | - | Gm36408 | NNNCAATAATAACTTGGTCA | NGG | 2 | 0.1222 | Tier III |
116 | NC_000077.6 | 11 | 55839758 | - | LOC105244170 | NNNCAATAATAACTTGGTCA | NGG | 2 | 0.1222 | Tier III |
117 | NC_000083.6 | 17 | 9383161 | + | Gm7947 | NNNCAATAATAACTTGGTCA | NGG | 2 | 0.1222 | Tier III |
118 | NC_000083.6 | 17 | 90487679 | + | Gm32337 | NNNCAATAATAACTTGGTCA | NGG | 2 | 0.1222 | Tier III |
119 | NC_000074.6 | 8 | 41887264 | + | 2810404M03Rik | NNNAAATAATAACCTGGTCA | NGG | 2 | 0.0989 | Tier III |
120 | NC_000067.6 | 1 | 157503844 | - | Gm15486 | NNNAAATAATAACTTGTTCA | NGG | 2 | 0.0865 | Tier III |
121 | NC_000067.6 | 1 | 79052504 | - | Gm29536 | NNNAAATAATAACTTGCTCA | NGG | 2 | 0.0814 | Tier III |
122 | NC_000079.6 | 13 | 43257751 | + | LOC115488162 | NNNACATAATAACTTTGTCA | NGA | 2 | 0.0347 | Tier III |
123 | NC_000073.6 | 7 | 136289950 | + | C030029H02Rik | NNNAAATAATAAATTTGTCA | NGA | 2 | 0.0267 | Tier III |