Construct: sgRNA BRDN0001146417

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
AGTCAGACATCAAGACACAC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CLK3 (1198)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77286

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000015.10 15 74625808 - CLK3 NNNCAGACATCAAGACACAC NGG 0 1.0 Tier I
2 NC_000017.11 17 75120403 - ARMC7 NNNCAGACATCAGGACACAC NGG 1 0.6522 Tier II
3 NC_000002.12 2 32603364 - BIRC6 NNNCAGAAATCAAGAAACAC NGG 2 0.65 Tier II
4 NC_000009.12 9 4434716 - GLIS3 NNNCAAACTTCAAGACACAC NGG 2 0.6 Tier II
5 NC_000020.11 20 25446484 - GINS1 NNNCAGAAAACAAGACACAC NGG 2 0.5571 Tier II
6 NC_000010.11 10 51098900 + PRKG1 NNNCAGACATCAAGAAAAAC NGG 2 0.5385 Tier II
7 NC_000015.10 15 58464683 + LIPC NNNCACACATCAAAACACAC NGG 2 0.5114 Tier II
8 NC_000001.11 1 75160411 - LHX8 NNNCATACATCAAAACACAC NGG 2 0.5 Tier II
9 NC_000014.9 14 105282098 + BRF1 NNNCAGAGATCAAAACACAC NGG 2 0.4821 Tier II
10 NC_000007.14 7 8593669 - NXPH1 NNNAAGACATCAAGACAAAC NGG 2 0.4534 Tier II
11 NC_000010.11 10 93423946 + MYOF NNNAAGACACCAAGACACAC NGG 2 0.4491 Tier II
12 NC_000007.14 7 6143173 - USP42 NNNCACACATCAAGACACTC NGG 2 0.3671 Tier II
13 NC_000002.12 2 23421642 - KLHL29 NNNCACACACCAAGACACAC NGG 2 0.3636 Tier II
14 NC_000009.12 9 91904663 + ROR2 NNNCAGGCAGCAAGACACAC NGG 2 0.3529 Tier II
15 NC_000006.12 6 15510619 - JARID2 NNNCATACAGCAAGACACAC NGG 2 0.3333 Tier II
16 NC_000019.10 19 41115480 - CYP2F1 NNNGAGAGATCAAGACACAC NGG 2 0.3214 Tier II
17 NC_000018.10 18 9755265 - RAB31 NNNCAGACATCAAGACAAAA NGG 2 0.2692 Tier II
18 NC_000020.11 20 63983281 - PRPF6 NNNCAGACACCAACACACAC NGG 2 0.2286 Tier II
19 NC_000001.11 1 186178131 - HMCN1 NNNCAGACAACAAGACACAC NAG 2 0.2222 Tier II
20 NC_000019.10 19 11022435 - SMARCA4 NNNCAGACATTGAGACACAC NGG 2 0.2222 Tier II
21 NC_000003.12 3 143533382 + SLC9A9 NNNCTGACCTCAAGACACAC NGG 2 0.2078 Tier II
22 NC_000004.12 4 8459982 + TRMT44 NNNCAGACATCAAGACACCC NGG 1 0.2069 Tier II
23 NC_000012.12 12 129567932 - TMEM132D NNNCAGACATCAAGACATAT NGG 2 0.1929 Tier II
24 NC_000002.12 2 96972387 + FAM178B NNNCAGACGTCAAGCCACAC NGG 2 0.1818 Tier II
25 NC_000005.10 5 76493394 - IQGAP2 NNNCAGACATGGAGACACAC NGG 2 0.1806 Tier II
26 NC_000002.12 2 6879741 - RSAD2 NNNCAGGCATGAAGACACAC NGG 2 0.1765 Tier II
27 NC_000002.12 2 26979125 + MAPRE3 NNNCAGAGATGAAGACACAC NGG 2 0.1607 Tier II
28 NC_000021.9 21 33893961 + ITSN1 NNNCAGACACCAAGACACAC NAG 2 0.1383 Tier II
29 NC_000017.11 17 27580086 + KSR1 NNNCACACATCAAGACCCAC NGG 2 0.1203 Tier II
30 NC_000023.11 X 151633532 + PASD1 NNNCAGACATCAAAAGACAC NGG 2 0.1154 Tier II
31 NC_000014.9 14 100065728 - EVL NNNCAGGCATCAAGAGACAC NGG 2 0.1086 Tier II
32 NC_000001.11 1 16998653 + ATP13A2 NNNCACACATCAAGAGACAC NGG 2 0.1049 Tier II
33 NC_000007.14 7 147789508 + CNTNAP2 NNNCAGACATCCAGCCACAC NGG 2 0.0718 Tier II
34 NC_000010.11 10 70414445 - EIF4EBP2 NNNCAGACAACAAGACACAC NGA 2 0.0595 Tier II
35 NC_000019.10 19 33406209 - PEPD NNNCAGACATCATGAGACAC NGG 2 0.0462 Tier II
36 NC_000011.10 11 73348375 + ARHGEF17 NNNCAGACATCAACACACAG NGG 2 0.0252 Tier II
37 NC_000002.12 2 37103808 + EIF2AK2 NNNCAGACATCATGACACAC NTG 2 0.0117 Tier II
38 NC_000002.12 2 105801113 - NCK2 NNNCAGACATCAAGCCACAC NGC 2 0.0061 Tier II
39 NC_000004.12 4 99214019 - ADH6 NNNCAGACATCAAGTCACAC NGC 2 0.0044 Tier II
40 NC_000003.12 3 139290155 - LOC124906290 NNNCAGGCATAAAGACACAC NGG 2 0.5294 Tier III
41 NC_000015.10 15 58464683 + LIPC-AS1 NNNCACACATCAAAACACAC NGG 2 0.5114 Tier III
42 NC_000007.14 7 8593669 - LOC105375144 NNNAAGACATCAAGACAAAC NGG 2 0.4534 Tier III
43 NC_000010.11 10 64536965 - LOC124902439 NNNCAGAAATCAAGACAAAC NGG 2 0.35 Tier III
44 NC_000008.11 8 78861123 - MITA1 NNNCAGACAGCAACACACAC NGG 2 0.2143 Tier III
45 NC_000006.12 6 129909210 - LOC105377999 NNNCAGACATCAGGACTCAC NGG 2 0.087 Tier III
46 NC_000019.10 19 33406209 - LOC124904692 NNNCAGACATCATGAGACAC NGG 2 0.0462 Tier III
47 NC_000006.12 6 90741607 - LOC107986623 NNNCACACATCAAGACACAG NGG 2 0.0401 Tier III
48 NC_000007.14 7 156604211 + RNF32-DT NNNCAGACATCAAGAAACAC NGC 2 0.0222 Tier III
49 NC_000024.10 Y 23476045 + REREP1Y NNNCAGACATCAACACACAC NTG 2 0.0167 Tier III
50 NC_000024.10 Y 26194567 - REREP2Y NNNCAGACATCAACACACAC NTG 2 0.0167 Tier III
51 NC_000007.14 7 142582321 - TRB NNNCAGACCTCAAGACACAC NGT 2 0.0092 Tier III
52 NC_000013.11 13 63622265 - LOC105370236 NNNCTGACATCAAGACACAC NGC 2 0.0081 Tier III
53 NC_000004.12 4 99214019 - LOC100507053 NNNCAGACATCAAGTCACAC NGC 2 0.0044 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000075.6 9 57754663 + Clk3 NNNCAGACATCAGGACACAA NGG 2 0.3261 Tier I
2 NC_000070.6 4 155657852 + Mib2 NNNCAGACATTAAGACTCAC NGG 2 0.041 Tier I
3 NC_000068.7 2 24679478 - Cacna1b NNNCAGACATGAAGACACAC NTG 2 0.0097 Tier I
4 NC_000071.6 5 9712601 + Grm3 NNNCAGACATCAAGACACAC NGG 0 1.0 Tier II
5 NC_000085.6 19 22922741 - Trpm3 NNNCAGACATCAAGGCACAC NGG 1 0.65 Tier II
6 NC_000070.6 4 155901301 + Acap3 NNNCAGACAACAGGACACAC NGG 2 0.559 Tier II
7 NC_000074.6 8 77150905 - Nr3c2 NNNTAGACATCAAGGCACAC NGG 2 0.52 Tier II
8 NC_000071.6 5 112717805 + Myo18b NNNGAGACAACAAGACACAC NGG 2 0.4286 Tier II
9 NC_000073.6 7 117434215 - Xylt1 NNNCAGACACAAAGACACAC NGG 2 0.4 Tier II
10 NC_000082.6 16 41633592 + Lsamp NNNCAGACACCGAGACACAC NGG 2 0.3852 Tier II
11 NC_000084.6 18 31301619 - Rit2 NNNCAGACAGAAAGACACAC NGG 2 0.375 Tier II
12 NC_000086.7 X 94197938 - Eif2s3x NNNCAGACATCAAAACACAA NGG 2 0.375 Tier II
13 NC_000076.6 10 83250306 - Slc41a2 NNNCAGGCAGCAAGACACAC NGG 2 0.3529 Tier II
14 NC_000071.6 5 149204825 - Uspl1 NNNCAGACATTAAGACACAC NGG 1 0.3077 Tier II
15 NC_000067.6 1 36661139 + Fam178b NNNCAGACATCAAGCCACAC NGG 1 0.2727 Tier II
16 NC_000083.6 17 35967635 + Abcf1 NNNCAGCCTTCAAGACACAC NGG 2 0.2625 Tier II
17 NC_000077.6 11 6048838 + Camk2b NNNCAGACATCAAACCACAC NGG 2 0.2045 Tier II
18 NC_000067.6 1 151675000 + Niban1 NNNCAGACATACAGACACAC NGG 2 0.1974 Tier II
19 NC_000070.6 4 135529042 + Stpg1 NNNCAGACATACAGACACAC NGG 2 0.1974 Tier II
20 NC_000071.6 5 130923385 + Galnt17 NNNCAGACATACAGACACAC NGG 2 0.1974 Tier II
21 NC_000068.7 2 150120211 + Zfp120 NNNCAGACATAAAGACACAC NAG 2 0.1944 Tier II
22 NC_000069.6 3 86315203 - Lrba NNNCAGACATCAAAACACAC NAG 2 0.1944 Tier II
23 NC_000078.6 12 116206572 - Wdr60 NNNCACACATCAAGCCACAC NGG 2 0.186 Tier II
24 NC_000082.6 16 92806549 + Runx1 NNNCACACATCCAGACACAC NGG 2 0.1794 Tier II
25 NC_000067.6 1 84910814 + Slc16a14 NNNCAGACACCTAGACACAC NGG 2 0.1778 Tier II
26 NC_000073.6 7 141101233 + Ano9 NNNCAGAAATCAATACACAC NGG 2 0.1733 Tier II
27 NC_000073.6 7 36724867 - Tshz3 NNNCAGACGTCAAGACACAC NAG 2 0.1728 Tier II
28 NC_000083.6 17 84417521 + Thada NNNCATACATCAAGACACAC NAG 2 0.1728 Tier II
29 NC_000070.6 4 139422605 - Ubr4 NNNCAGACACCATGACACAC NGG 2 0.16 Tier II
30 NC_000072.6 6 91483556 - Tmem43 NNNCAGACACCATGACACAC NGG 2 0.16 Tier II
31 NC_000073.6 7 135691722 - Mki67 NNNCAGACACCAAGACACAC NAG 2 0.1383 Tier II
32 NC_000067.6 1 176228151 + Pld5 NNNCAGACATCAAGACTCAC NGG 1 0.1333 Tier II
33 NC_000073.6 7 45784333 - Lmtk3 NNNCAGACATCAACACACAT NGG 2 0.1286 Tier II
34 NC_000072.6 6 99432333 + Foxp1 NNNCAGACAACAAGACAGAC NGG 2 0.1143 Tier II
35 NC_000083.6 17 15075984 + Ermard NNNCAGACATCCACACACAC NGG 2 0.1128 Tier II
36 NC_000070.6 4 12960985 - Triqk NNNCAGACATGAACACACAC NGG 2 0.1071 Tier II
37 NC_000077.6 11 65971051 - Dnah9 NNNCAGACATCTAGACACAC NAG 2 0.0864 Tier II
38 NC_000077.6 11 68776727 - Myh10 NNNCAGAGATCAAGACTCAC NGG 2 0.0857 Tier II
39 NC_000076.6 10 85983238 + Bpifc NNNCAGACATGAAGACACAT NGG 2 0.075 Tier II
40 NC_000083.6 17 12252448 - Map3k4 NNNCAGACACCAAGACAGAC NGG 2 0.0711 Tier II
41 NC_000083.6 17 68513223 - L3mbtl4 NNNCAGACATCAAGACACAC NGA 1 0.0694 Tier II
42 NC_000077.6 11 47663056 - Sgcd NNNCAGACATCCAGACACAC NAG 2 0.0682 Tier II
43 NC_000078.6 12 91201815 + Cep128 NNNCAGACATCAACAGACAC NGG 2 0.0659 Tier II
44 NC_000071.6 5 118227591 + Rnft2 NNNCAGACATGAAGACACAC NAG 2 0.0648 Tier II
45 NC_000067.6 1 87993450 + Usp40 NNNCAGACATCAAGACAAAC NGA 2 0.0374 Tier II
46 NC_000077.6 11 30014637 + Eml6 NNNCAGACTTCAAGACACAG NGG 2 0.0353 Tier II
47 NC_000078.6 12 76082044 - Syne2 NNNCAGACAGCAAGACACAC NGA 2 0.0347 Tier II
48 NC_000068.7 2 73721273 + Chn1 NNNCAGACATTAAGACACAC NGA 2 0.0214 Tier II
49 NC_000075.6 9 18458090 - Zfp558 NNNCAGACATCAACACACAC NTG 2 0.0167 Tier II
50 NC_000078.6 12 98693464 + Ptpn21 NNNCAGACATCAACACACAC NTG 2 0.0167 Tier II
51 NC_000084.6 18 41994910 + Grxcr2 NNNCAGACATCAAGACACCC NTG 2 0.0081 Tier II
52 NC_000076.6 10 80127203 - Stk11 NNNCAGACATCAATACACAC NGC 2 0.0059 Tier II
53 NC_000085.6 19 59292481 + Slc18a2 NNNCAGACATCAAGACAGAC NGC 2 0.003 Tier II
54 NC_000067.6 1 174536117 - Fmn2 NNNCAGACATCAAGACACAC NAA 2 0.0 Tier II
55 NC_000069.6 3 117989815 + Gm32344 NNNCAGAAATCAAAACACAC NGG 2 0.4875 Tier III
56 NC_000072.6 6 68576616 + Igk NNNCATACATCAGGACACAC NGG 2 0.4348 Tier III
57 NC_000069.6 3 140958809 - Gm32875 NNNAAGACATCAAGACACAT NGG 2 0.2526 Tier III
58 NC_000079.6 13 44019321 - Gm33489 NNNCACACATCTAGACACAC NGG 2 0.2273 Tier III
59 NC_000073.6 7 93001727 + Gm31663 NNNCAGACATACAGACACAC NGG 2 0.1974 Tier III
60 NC_000074.6 8 13751058 - Gm46032 NNNCAGACACCAAGCCACAC NGG 2 0.1455 Tier III
61 NC_000073.6 7 45784333 - Gm5897 NNNCAGACATCAACACACAT NGG 2 0.1286 Tier III
62 NC_000072.6 6 17143622 + Gm4876 NNNCAGACATGAAGACAGAC NGG 2 0.0333 Tier III
63 NC_000075.6 9 30992355 - Gm29724 NNNCAGACATTAAGACACAG NGG 2 0.0181 Tier III
64 NC_000070.6 4 114336392 + Gm40228 NNNCAGACATCAAGACCCAC NGA 2 0.0123 Tier III
65 NC_000085.6 19 19950396 - Gm32469 NNNCAGCCATCAAGACACAC NGT 2 0.0071 Tier III
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Other clones with same target sequence:

(none)