Construct: sgRNA BRDN0001146428

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
CAGGGCAAGACCATCAACCT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PIP5KL1 (138429)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
75823

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000009.12 9 127925872 - PIP5KL1 NNNGGCAAGACCATCAACCT NGG 0 1.0 Tier I
2 NC_000014.9 14 73262694 + PAPLN NNNGGCAAGACCAACAGCCT NGG 2 0.1092 Tier I
3 NC_000023.11 X 1593768 + AKAP17A NNNGGCAAGACCATCAAGCT NAG 2 0.0346 Tier I
4 NC_000024.10 Y 1593768 + AKAP17A NNNGGCAAGACCATCAAGCT NAG 2 0.0346 Tier I
5 NC_000012.12 12 124913047 - UBC NNNGGCAAGACCATCACCCT NGA 2 0.0123 Tier I
6 NC_000012.12 12 124913503 - UBC NNNGGCAAGACCATCACCCT NGA 2 0.0123 Tier I
7 NC_000017.11 17 16381949 + UBB NNNGGCAAGACCATCACCCT NGA 2 0.0123 Tier I
8 NC_000017.11 17 16382177 + UBB NNNGGCAAGACCATCACCCT NGA 2 0.0123 Tier I
9 NC_000006.12 6 4760080 + CDYL NNNGGCTAGTCCATCAACCT NGG 2 0.386 Tier II
10 NC_000002.12 2 96932080 + FAM178B NNNGGGAAGACAATCAACCT NGG 2 0.3571 Tier II
11 NC_000005.10 5 41169121 + C6 NNNGGCAGGACCATCAACAT NGG 2 0.3143 Tier II
12 NC_000017.11 17 45788226 + CRHR1 NNNGGAAAGACCATCAGCCT NGG 2 0.1639 Tier II
13 NC_000017.11 17 45788226 + LINC02210-CRHR1 NNNGGAAAGACCATCAGCCT NGG 2 0.1639 Tier II
14 NC_000012.12 12 66589961 - GRIP1 NNNGGCTAGACCTTCAACCT NGG 2 0.1313 Tier II
15 NC_000006.12 6 155118078 - TIAM2 NNNGGCACGACCATCAACCT NAG 2 0.1111 Tier II
16 NC_000002.12 2 236008689 - AGAP1 NNNGGAAAGACCATCAACCC NGG 2 0.0844 Tier II
17 NC_000010.11 10 26501148 + APBB1IP NNNGGCAGGACCATCAACCT NTG 2 0.0286 Tier II
18 NC_000001.11 1 217850593 - SPATA17 NNNGGCAAGACCATCACCCT NGA 2 0.0123 Tier II
19 NC_000008.11 8 123236893 + C8orf76 NNNGGCAAGACCATCACCCT NGA 2 0.0123 Tier II
20 NC_000008.11 8 123236893 + ZHX1-C8orf76 NNNGGCAAGACCATCACCCT NGA 2 0.0123 Tier II
21 NC_000014.9 14 58141321 + ARMH4 NNNGGCAAGACCATCACCCT NGA 2 0.0123 Tier II
22 NC_000023.11 X 130021738 - BCORL1 NNNGGCAAGACCATGAACCT NGA 2 0.0035 Tier II
23 NC_000007.14 7 43269628 + HECW1 NNNGGCAAGACCATCCATCT NGG 2 0.0 Tier II
24 NC_000010.11 10 98445299 + HPS1 NNNGGCAAGATCATCTACCT NGG 2 0.0 Tier II
25 NC_000005.10 5 168227465 + TENM2 NNNGGCTAGACCAGCAACCT NGG 2 0.0 Tier II
26 NC_000007.14 7 30723217 + LOC105375219 NNNGCCAAGACCATCAACCA NGG 2 0.442 Tier III
27 NC_000007.14 7 30723217 + LOC105375220 NNNGCCAAGACCATCAACCA NGG 2 0.442 Tier III
28 NC_000016.10 16 58807684 - LOC107984867 NNNGGCTAGACCATCAATCT NGG 2 0.2812 Tier III
29 NC_000005.10 5 152118350 + LINC01933 NNNGGCAAGTCCTTCAACCT NGG 2 0.2647 Tier III
30 NC_000001.11 1 217850593 - UBBP2 NNNGGCAAGACCATCACCCT NGA 2 0.0123 Tier III
31 NC_000002.12 2 136329645 - UBBP1 NNNGGCAAGACCATCACCCT NGA 2 0.0123 Tier III
32 NC_000002.12 2 148863761 - UBBP3 NNNGGCAAGACCATCACCCT NGA 2 0.0123 Tier III
33 NC_000007.14 7 25689919 - UBA52P1 NNNGGCAAGACCATCACCCT NGA 2 0.0123 Tier III
34 NC_000007.14 7 25689919 - LINC03007 NNNGGCAAGACCATCACCCT NGA 2 0.0123 Tier III
35 NC_000008.11 8 123236893 + UBA52P5 NNNGGCAAGACCATCACCCT NGA 2 0.0123 Tier III
36 NC_000009.12 9 22012196 + CDKN2B-AS1 NNNGGCAAGACCATCACCCT NGA 2 0.0123 Tier III
37 NC_000009.12 9 22012196 + UBA52P6 NNNGGCAAGACCATCACCCT NGA 2 0.0123 Tier III
38 NC_000014.9 14 58141321 + UBA52P3 NNNGGCAAGACCATCACCCT NGA 2 0.0123 Tier III
39 NC_000017.11 17 22204362 + UBBP4 NNNGGCAAGACCATCACCCT NGA 2 0.0123 Tier III
40 NC_000017.11 17 22204590 + UBBP4 NNNGGCAAGACCATCACCCT NGA 2 0.0123 Tier III
41 NC_000005.10 5 168227465 + LOC124901128 NNNGGCTAGACCAGCAACCT NGG 2 0.0 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000071.6 5 125386169 - Ubc NNNGGCAAGACCATCACCCT NGA 2 0.0123 Tier I
2 NC_000071.6 5 125386397 - Ubc NNNGGCAAGACCATCACCCT NGA 2 0.0123 Tier I
3 NC_000071.6 5 125386625 - Ubc NNNGGCAAGACCATCACCCT NGA 2 0.0123 Tier I
4 NC_000071.6 5 125386853 - Ubc NNNGGCAAGACCATCACCCT NGA 2 0.0123 Tier I
5 NC_000071.6 5 125387081 - Ubc NNNGGCAAGACCATCACCCT NGA 2 0.0123 Tier I
6 NC_000071.6 5 125387537 - Ubc NNNGGCAAGACCATCACCCT NGA 2 0.0123 Tier I
7 NC_000071.6 5 125387765 - Ubc NNNGGCAAGACCATCACCCT NGA 2 0.0123 Tier I
8 NC_000071.6 5 125387993 - Ubc NNNGGCAAGACCATCACCCT NGA 2 0.0123 Tier I
9 NC_000077.6 11 62552187 + Ubb NNNGGCAAGACCATCACCCT NGA 2 0.0123 Tier I
10 NC_000077.6 11 62552415 + Ubb NNNGGCAAGACCATCACCCT NGA 2 0.0123 Tier I
11 NC_000077.6 11 62552643 + Ubb NNNGGCAAGACCATCACCCT NGA 2 0.0123 Tier I
12 NC_000086.7 X 20645840 + Rbm10 NNNGGCAAGACCATCAACGT NGA 2 0.0087 Tier I
13 NC_000077.6 11 57135212 - Gria1 NNNGGCAAGTCCAACAACCT NGG 2 0.5462 Tier II
14 NC_000074.6 8 118171205 - Cdh13 NNNGGCAGGACAATCAACCT NGG 2 0.5238 Tier II
15 NC_000077.6 11 36040371 - Tenm2 NNNGGCTAGACCATCAACCT NGG 1 0.4375 Tier II
16 NC_000077.6 11 67988701 - Stx8 NNNAGCAAGACCACCAACCT NGG 2 0.2571 Tier II
17 NC_000079.6 13 48963345 + Fam120a NNNGGCAAGCCCAACAACCT NGG 2 0.2063 Tier II
18 NC_000070.6 4 119331975 - Ccdc30 NNNGGCAAGAGTATCAACCT NGG 2 0.1346 Tier II
19 NC_000085.6 19 39019153 - Cyp2c55 NNNGGCTAGACCATCAACCG NGG 2 0.0772 Tier II
20 NC_000070.6 4 36032916 - Lingo2 NNNGGCAAGACCATCACCAT NGG 2 0.0756 Tier II
21 NC_000071.6 5 112957865 + Grk3 NNNGGCAAGACCATGAACTT NGG 2 0.0231 Tier II
22 NC_000081.6 15 71924299 - Col22a1 NNNGGCAAGACCATCATGCT NGG 2 0.0178 Tier II
23 NC_000075.6 9 80445780 + Impg1 NNNGGCAAGACCATTATCCT NGG 2 0.0089 Tier II
24 NC_000070.6 4 117376687 + Rnf220 NNNGGCAAGACCACCAACCT NGC 2 0.0063 Tier II
25 NC_000082.6 16 34189415 - Kalrn NNNGGCAAGACCAGCACCCT NGG 2 0.0 Tier II
26 NC_000069.6 3 5750945 + Gm8797 NNNGGCAAGACCATCACCCT NGA 2 0.0123 Tier III
27 NC_000080.6 14 46084167 + Ubb-ps NNNGGCAAGACCATCACCCT NGA 2 0.0123 Tier III
28 NC_000080.6 14 46084395 + Ubb-ps NNNGGCAAGACCATCACCCT NGA 2 0.0123 Tier III
29 NC_000080.6 14 46084623 + Ubb-ps NNNGGCAAGACCATCACCCT NGA 2 0.0123 Tier III
30 NC_000070.6 4 102048224 - Gm12799 NNNGGCAAGACCATCAACGT NGA 2 0.0087 Tier III
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Other clones with same target sequence:

(none)