GPP Web Portal - Clone Details

Construct: sgRNA BRDN0001146432

Construct Description:

Construct Type:: sgRNA
Other Identifiers:: n/a
DNA Barcode:: TCCTTATTGTGATCTCCATG

Original Target:

Taxon:: Homo sapiens (human)
Gene:: CDK11B (984)
Gene Description:: n/a
CRISPR Mechanism: CRISPRko

Vector Information:

Vector Backbone:: pXPR_003
Pol II Cassette 1:: EF1A-PuroR
Pol II Cassette 2:: n/a
Selection Marker:: PuroR
Visible Reporter:: n/a
Epitope Tag:: n/a

Additional Information:

Addgene ID:: 77063

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

	Reference Sequence	Chromosome	Cut Position	Match Strand	Match Gene	Match Sequence	PAM Seq.	Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases.	CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016.	Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works.
1	NC_000001.11	1	1655423	+	CDK11B	NNNTTATTGTGATCTCCATG	NGG	0	1.0	Tier I
2	NC_000001.11	1	1721650	+	CDK11A	NNNTTATTGTGATCTCCATG	NAG	1	0.2593	Tier I
3	NC_000006.12	6	54157549	+	MLIP	NNNTTATTGTGACCTCCATG	NGG	1	0.7895	Tier II
4	NC_000010.11	10	76283688	+	LRMDA	NNNTTATTGTGATCTCCATT	NGG	1	0.7	Tier II
5	NC_000019.10	19	3498193	+	DOHH	NNNTTGCTGTGATCTCCATG	NGG	2	0.4911	Tier II
6	NC_000014.9	14	73179078	+	PSEN1	NNNTTTCTGTGATCTCCATG	NGG	2	0.4911	Tier II
7	NC_000012.12	12	113205144	+	IQCD	NNNTTACTGTGAACTCCATG	NGG	2	0.476	Tier II
8	NC_000016.10	16	17358631	-	XYLT1	NNNTTTGTGTGATCTCCATG	NGG	2	0.4202	Tier II
9	NC_000003.12	3	134195294	-	RYK	NNNTTATTGTGATCTCCGTA	NGG	2	0.375	Tier II
10	NC_000023.11	X	20198179	-	RPS6KA3	NNNTTATTGTCATTTCCATG	NGG	2	0.3143	Tier II
11	NC_000023.11	X	32155751	+	DMD	NNNTTATTGTGAGCTCCATG	NGG	1	0.2609	Tier II
12	NC_000020.11	20	35798952	+	PHF20	NNNTTAATGTGAGCTCCATG	NGG	2	0.2283	Tier II
13	NC_000002.12	2	11236197	-	ROCK2	NNNGTATTGTGTTCTCCATG	NGG	2	0.2083	Tier II
14	NC_000009.12	9	83492292	-	FRMD3	NNNTTATTGTCATCTCCATG	NAG	2	0.1111	Tier II
15	NC_000011.10	11	132948330	+	OPCML	NNNTTATTGTGATTTCCATG	NGA	2	0.0509	Tier II
16	NC_000017.11	17	1971129	+	RTN4RL1	NNNTTATTGTAATCTCCATG	NGT	2	0.0161	Tier II
17	NC_000020.11	20	20493831	-	RALGAPA2	NNNTTATTCTGATCTCCATG	NGC	2	0.012	Tier II
18	NC_000001.11	1	202530681	+	PPP1R12B	NNNTTATTGTGAGCTCCATG	NTG	2	0.0102	Tier II
19	NC_000020.11	20	16363220	-	KIF16B	NNNTTATTGTGATCTCCATC	NGC	2	0.0095	Tier II
20	NC_000017.11	17	75972799	-	ACOX1	NNNTTATTGTCATCTCCATG	NGT	2	0.0069	Tier II
21	NC_000006.12	6	64370283	+	EYS	NNNCTATTGTGATGTCCATG	NGG	2	0.0	Tier II
22	NC_000023.11	X	108305463	+	COL4A6	NNNTTATTGTGAAGTCCATG	NGG	2	0.0	Tier II
23	NC_000006.12	6	54157549	+	LOC105375097	NNNTTATTGTGACCTCCATG	NGG	1	0.7895	Tier III
24	NC_000008.11	8	63280427	+	LOC105375873	NNNTTATTGTGATTTCCATG	NGG	1	0.7333	Tier III
25	NC_000010.11	10	76283688	+	LOC124902462	NNNTTATTGTGATCTCCATT	NGG	1	0.7	Tier III
26	NC_000019.10	19	3498193	+	LOC105372245	NNNTTGCTGTGATCTCCATG	NGG	2	0.4911	Tier III
27	NC_000015.10	15	24422160	-	LOC105370733	NNNTTATTGTGACCACCATG	NGG	2	0.4571	Tier III
28	NC_000015.10	15	24472058	-	LOC105370733	NNNTTATTGTGACCACCATG	NGG	2	0.4571	Tier III
29	NC_000005.10	5	159619021	+	LOC105377684	NNNTTAATGTCATCTCCATG	NGG	2	0.375	Tier III
30	NC_000023.11	X	151904466	+	MAGEA4-AS1	NNNTTATTTTGATCACCATG	NGG	2	0.3088	Tier III
31	NC_000008.11	8	8208477	+	ENPP7P1	NNNCTATTGTGATCTGCATG	NGG	2	0.0995	Tier III
32	NC_000008.11	8	8208477	+	FAM85B	NNNCTATTGTGATCTGCATG	NGG	2	0.0995	Tier III
33	NC_000008.11	8	12457208	-	FAM86B2-DT	NNNCTATTGTGATCTGCATG	NGG	2	0.0995	Tier III
34	NC_000008.11	8	12457208	-	ENPP7P6	NNNCTATTGTGATCTGCATG	NGG	2	0.0995	Tier III
35	NC_000008.11	8	56643447	-	LOC105375851	NNNTTAATGTGATCTCCATG	NCG	2	0.0937	Tier III

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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

	Reference Sequence	Chromosome	Cut Position	Match Strand	Match Gene	Match Sequence	PAM Seq.	Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases.	CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016.	Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works.
1	NC_000070.6	4	155626836	-	Cdk11b	NNNTGATTGTGATCTCCATT	NGG	2	0.448	Tier I
2	NC_000077.6	11	119733331	-	Rptor	NNNTTAGTGTGGTCTCCATG	NGG	2	0.4248	Tier II
3	NC_000083.6	17	40952169	+	Mmut	NNNTCATTGTGCTCTCCATG	NGG	2	0.2632	Tier II
4	NC_000072.6	6	85679836	-	Alms1	NNNGTATTGTGATCTCCATG	NAG	2	0.162	Tier II
5	NC_000078.6	12	10465997	+	Gm38407	NNNTTATTGTGTTCTCCATA	NGG	2	0.3125	Tier III
6	NC_000080.6	14	114861299	-	4930524C18Rik	NNNTTATTGTGAGCTCCATG	NTG	2	0.0102	Tier III

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Other clones with same target sequence:

(none)