Construct: sgRNA BRDN0001146448

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
GAACCATATGAAACTAGCCA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TLK2 (11011)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
75615

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000017.11 17 62522269 + TLK2 NNNCCATATGAAACTAGCCA NGG 0 1.0 Tier I
2 NC_000010.11 10 37820628 - ZNF248 NNNCCACATGAAACTAGCCA NGG 1 0.6875 Tier II
3 NC_000002.12 2 162636473 - KCNH7 NNNGCATAAGAAACTAGCCA NGG 2 0.4643 Tier II
4 NC_000016.10 16 76343968 + CNTNAP4 NNNCCAGATGGAACTAGCCA NGG 2 0.3824 Tier II
5 NC_000005.10 5 107985078 - FBXL17 NNNCTAGATGAAACTAGCCA NGG 2 0.3743 Tier II
6 NC_000009.12 9 137488379 - PNPLA7 NNNCCATATGGAACTAGCAA NGG 2 0.2786 Tier II
7 NC_000002.12 2 115335800 + DPP10 NNNCCATATGACACTAACCA NGG 2 0.2456 Tier II
8 NC_000003.12 3 189758845 + TP63 NNNCCATTTGAAACTAGACA NGG 2 0.2308 Tier II
9 NC_000009.12 9 132522190 + CFAP77 NNNCTATATGAAAATAGCCA NGG 2 0.2227 Tier II
10 NC_000010.11 10 29292228 + LYZL1 NNNCCTTATGACACTAGCCA NGG 2 0.188 Tier II
11 NC_000010.11 10 30626556 - LYZL2 NNNCCTTATGACACTAGCCA NGG 2 0.188 Tier II
12 NC_000020.11 20 34530962 - DYNLRB1 NNNCCATTTGAATCTAGCCA NGG 2 0.1286 Tier II
13 NC_000003.12 3 75951470 + ROBO2 NNNCCATATGAAACAAGCCA NTG 2 0.0226 Tier II
14 NC_000022.11 22 38747609 + SUN2 NNNTCATATGAAACTAGCCA NGC 2 0.0178 Tier II
15 NC_000006.12 6 146390809 + GRM1 NNNTCATATGAAACTAGCCA NGT 2 0.0129 Tier II
16 NC_000017.11 17 34039655 - ASIC2 NNNCCATATGAAAGTAGCCA NGG 1 0.0 Tier II
17 NC_000010.11 10 37820628 - TLK2P2 NNNCCACATGAAACTAGCCA NGG 1 0.6875 Tier III
18 NC_000005.10 5 101849580 + LOC105379102 NNNCAATATGATACTAGCCA NGG 2 0.1905 Tier III
19 NC_000017.11 17 43383399 + LINC00910 NNNACATATGAAACTAGGCA NGG 2 0.1123 Tier III
20 NC_000007.14 7 26470717 - LINC02981 NNNCCATCTGAAACTATCCA NGG 2 0.1071 Tier III
21 NC_000017.11 17 34039655 - TLK2P1 NNNCCATATGAAAGTAGCCA NGG 1 0.0 Tier III
22 NC_000017.11 17 34039655 - LOC107987247 NNNCCATATGAAAGTAGCCA NGG 1 0.0 Tier III
Download CSV

Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000077.6 11 105208917 + Tlk2 NNNCCATATGACACTAGCCA NGG 1 0.2632 Tier I
2 NC_000072.6 6 23117220 + Aass NNNCAATATAAAACTAGCCA NGG 2 0.5333 Tier II
3 NC_000074.6 8 89040780 - Sall1 NNNCCAGATGAAATTAGCCA NGG 2 0.4314 Tier II
4 NC_000070.6 4 151509124 - Camta1 NNNCCATGTGAAACAAGCCA NGG 2 0.4246 Tier II
5 NC_000073.6 7 144509199 + Ppfia1 NNNTCCTATGAAACTAGCCA NGG 2 0.3636 Tier II
6 NC_000075.6 9 110143166 + Smarcc1 NNNCCATAGGAAACAAGCCA NGG 2 0.3584 Tier II
7 NC_000072.6 6 146305911 + Itpr2 NNNCCTTTTGAAACTAGCCA NGG 2 0.3061 Tier II
8 NC_000070.6 4 147125474 + Zfp978 NNNCCATATGACACTAGCCA NGG 1 0.2632 Tier II
9 NC_000077.6 11 36384695 + Tenm2 NNNCCATATGAAACAAGCAA NGG 2 0.2481 Tier II
10 NC_000067.6 1 130048811 + Thsd7b NNNCCATTTGAAACTAGACA NGG 2 0.2308 Tier II
11 NC_000076.6 10 67201717 - Jmjd1c NNNCTATATGAAACTAGCCA NAG 2 0.165 Tier II
12 NC_000070.6 4 146604431 + Zfp993 NNNCCATATGACACTAGCAA NGG 2 0.1128 Tier II
13 NC_000070.6 4 147443686 + Zfp982 NNNCCATATGACACTAGCAA NGG 2 0.1128 Tier II
14 NC_000079.6 13 119698035 + Hmgcs1 NNNCCATATGAAAACAGCCA NGG 2 0.0955 Tier II
15 NC_000069.6 3 28428120 + Tnik NNNCCATATGAAAATATCCA NGG 2 0.0875 Tier II
16 NC_000068.7 2 5030625 + Optn NNNCCATATGAAGCTAGGCA NGG 2 0.087 Tier II
17 NC_000075.6 9 51946823 - Fdx1 NNNACATATGAAACTAGCCA NGT 2 0.0136 Tier II
18 NC_000070.6 4 146263846 + Vmn2r-ps17 NNNCCATATGACACTAGCCA NGG 1 0.2632 Tier III
19 NC_000077.6 11 36384695 + Gm33585 NNNCCATATGAAACAAGCAA NGG 2 0.2481 Tier III
20 NC_000068.7 2 168341742 - Gm33262 NNNCCATATGAATTTAGCCA NGG 2 0.22 Tier III
21 NC_000070.6 4 145774938 + Vmn2r-ps15 NNNCCATATGACACTAGCAA NGG 2 0.1128 Tier III
22 NC_000070.6 4 145774938 + Gm13243 NNNCCATATGACACTAGCAA NGG 2 0.1128 Tier III
23 NC_000070.6 4 146604431 + Vmn2r-ps16 NNNCCATATGACACTAGCAA NGG 2 0.1128 Tier III
24 NC_000070.6 4 146604431 + Gm13240 NNNCCATATGACACTAGCAA NGG 2 0.1128 Tier III
25 NC_000070.6 4 147443686 + Gm13161 NNNCCATATGACACTAGCAA NGG 2 0.1128 Tier III
Download CSV

Other clones with same target sequence:

(none)

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