Construct: sgRNA BRDN0001146448
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GAACCATATGAAACTAGCCA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- TLK2 (11011)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75615
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000017.11 |
17 |
62522269 |
+ |
TLK2 |
NNNCCATATGAAACTAGCCA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000010.11 |
10 |
37820628 |
- |
ZNF248 |
NNNCCACATGAAACTAGCCA |
NGG |
1 |
0.6875 |
Tier II |
| 3 |
NC_000002.12 |
2 |
162636473 |
- |
KCNH7 |
NNNGCATAAGAAACTAGCCA |
NGG |
2 |
0.4643 |
Tier II |
| 4 |
NC_000016.10 |
16 |
76343968 |
+ |
CNTNAP4 |
NNNCCAGATGGAACTAGCCA |
NGG |
2 |
0.3824 |
Tier II |
| 5 |
NC_000005.10 |
5 |
107985078 |
- |
FBXL17 |
NNNCTAGATGAAACTAGCCA |
NGG |
2 |
0.3743 |
Tier II |
| 6 |
NC_000009.12 |
9 |
137488379 |
- |
PNPLA7 |
NNNCCATATGGAACTAGCAA |
NGG |
2 |
0.2786 |
Tier II |
| 7 |
NC_000002.12 |
2 |
115335800 |
+ |
DPP10 |
NNNCCATATGACACTAACCA |
NGG |
2 |
0.2456 |
Tier II |
| 8 |
NC_000003.12 |
3 |
189758845 |
+ |
TP63 |
NNNCCATTTGAAACTAGACA |
NGG |
2 |
0.2308 |
Tier II |
| 9 |
NC_000009.12 |
9 |
132522190 |
+ |
CFAP77 |
NNNCTATATGAAAATAGCCA |
NGG |
2 |
0.2227 |
Tier II |
| 10 |
NC_000010.11 |
10 |
29292228 |
+ |
LYZL1 |
NNNCCTTATGACACTAGCCA |
NGG |
2 |
0.188 |
Tier II |
| 11 |
NC_000010.11 |
10 |
30626556 |
- |
LYZL2 |
NNNCCTTATGACACTAGCCA |
NGG |
2 |
0.188 |
Tier II |
| 12 |
NC_000020.11 |
20 |
34530962 |
- |
DYNLRB1 |
NNNCCATTTGAATCTAGCCA |
NGG |
2 |
0.1286 |
Tier II |
| 13 |
NC_000003.12 |
3 |
75951470 |
+ |
ROBO2 |
NNNCCATATGAAACAAGCCA |
NTG |
2 |
0.0226 |
Tier II |
| 14 |
NC_000022.11 |
22 |
38747609 |
+ |
SUN2 |
NNNTCATATGAAACTAGCCA |
NGC |
2 |
0.0178 |
Tier II |
| 15 |
NC_000006.12 |
6 |
146390809 |
+ |
GRM1 |
NNNTCATATGAAACTAGCCA |
NGT |
2 |
0.0129 |
Tier II |
| 16 |
NC_000017.11 |
17 |
34039655 |
- |
ASIC2 |
NNNCCATATGAAAGTAGCCA |
NGG |
1 |
0.0 |
Tier II |
| 17 |
NC_000010.11 |
10 |
37820628 |
- |
TLK2P2 |
NNNCCACATGAAACTAGCCA |
NGG |
1 |
0.6875 |
Tier III |
| 18 |
NC_000005.10 |
5 |
101849580 |
+ |
LOC105379102 |
NNNCAATATGATACTAGCCA |
NGG |
2 |
0.1905 |
Tier III |
| 19 |
NC_000017.11 |
17 |
43383399 |
+ |
LINC00910 |
NNNACATATGAAACTAGGCA |
NGG |
2 |
0.1123 |
Tier III |
| 20 |
NC_000007.14 |
7 |
26470717 |
- |
LINC02981 |
NNNCCATCTGAAACTATCCA |
NGG |
2 |
0.1071 |
Tier III |
| 21 |
NC_000017.11 |
17 |
34039655 |
- |
TLK2P1 |
NNNCCATATGAAAGTAGCCA |
NGG |
1 |
0.0 |
Tier III |
| 22 |
NC_000017.11 |
17 |
34039655 |
- |
LOC107987247 |
NNNCCATATGAAAGTAGCCA |
NGG |
1 |
0.0 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000077.6 |
11 |
105208917 |
+ |
Tlk2 |
NNNCCATATGACACTAGCCA |
NGG |
1 |
0.2632 |
Tier I |
| 2 |
NC_000072.6 |
6 |
23117220 |
+ |
Aass |
NNNCAATATAAAACTAGCCA |
NGG |
2 |
0.5333 |
Tier II |
| 3 |
NC_000074.6 |
8 |
89040780 |
- |
Sall1 |
NNNCCAGATGAAATTAGCCA |
NGG |
2 |
0.4314 |
Tier II |
| 4 |
NC_000070.6 |
4 |
151509124 |
- |
Camta1 |
NNNCCATGTGAAACAAGCCA |
NGG |
2 |
0.4246 |
Tier II |
| 5 |
NC_000073.6 |
7 |
144509199 |
+ |
Ppfia1 |
NNNTCCTATGAAACTAGCCA |
NGG |
2 |
0.3636 |
Tier II |
| 6 |
NC_000075.6 |
9 |
110143166 |
+ |
Smarcc1 |
NNNCCATAGGAAACAAGCCA |
NGG |
2 |
0.3584 |
Tier II |
| 7 |
NC_000072.6 |
6 |
146305911 |
+ |
Itpr2 |
NNNCCTTTTGAAACTAGCCA |
NGG |
2 |
0.3061 |
Tier II |
| 8 |
NC_000070.6 |
4 |
147125474 |
+ |
Zfp978 |
NNNCCATATGACACTAGCCA |
NGG |
1 |
0.2632 |
Tier II |
| 9 |
NC_000077.6 |
11 |
36384695 |
+ |
Tenm2 |
NNNCCATATGAAACAAGCAA |
NGG |
2 |
0.2481 |
Tier II |
| 10 |
NC_000067.6 |
1 |
130048811 |
+ |
Thsd7b |
NNNCCATTTGAAACTAGACA |
NGG |
2 |
0.2308 |
Tier II |
| 11 |
NC_000076.6 |
10 |
67201717 |
- |
Jmjd1c |
NNNCTATATGAAACTAGCCA |
NAG |
2 |
0.165 |
Tier II |
| 12 |
NC_000070.6 |
4 |
146604431 |
+ |
Zfp993 |
NNNCCATATGACACTAGCAA |
NGG |
2 |
0.1128 |
Tier II |
| 13 |
NC_000070.6 |
4 |
147443686 |
+ |
Zfp982 |
NNNCCATATGACACTAGCAA |
NGG |
2 |
0.1128 |
Tier II |
| 14 |
NC_000079.6 |
13 |
119698035 |
+ |
Hmgcs1 |
NNNCCATATGAAAACAGCCA |
NGG |
2 |
0.0955 |
Tier II |
| 15 |
NC_000069.6 |
3 |
28428120 |
+ |
Tnik |
NNNCCATATGAAAATATCCA |
NGG |
2 |
0.0875 |
Tier II |
| 16 |
NC_000068.7 |
2 |
5030625 |
+ |
Optn |
NNNCCATATGAAGCTAGGCA |
NGG |
2 |
0.087 |
Tier II |
| 17 |
NC_000075.6 |
9 |
51946823 |
- |
Fdx1 |
NNNACATATGAAACTAGCCA |
NGT |
2 |
0.0136 |
Tier II |
| 18 |
NC_000070.6 |
4 |
146263846 |
+ |
Vmn2r-ps17 |
NNNCCATATGACACTAGCCA |
NGG |
1 |
0.2632 |
Tier III |
| 19 |
NC_000077.6 |
11 |
36384695 |
+ |
Gm33585 |
NNNCCATATGAAACAAGCAA |
NGG |
2 |
0.2481 |
Tier III |
| 20 |
NC_000068.7 |
2 |
168341742 |
- |
Gm33262 |
NNNCCATATGAATTTAGCCA |
NGG |
2 |
0.22 |
Tier III |
| 21 |
NC_000070.6 |
4 |
145774938 |
+ |
Vmn2r-ps15 |
NNNCCATATGACACTAGCAA |
NGG |
2 |
0.1128 |
Tier III |
| 22 |
NC_000070.6 |
4 |
145774938 |
+ |
Gm13243 |
NNNCCATATGACACTAGCAA |
NGG |
2 |
0.1128 |
Tier III |
| 23 |
NC_000070.6 |
4 |
146604431 |
+ |
Vmn2r-ps16 |
NNNCCATATGACACTAGCAA |
NGG |
2 |
0.1128 |
Tier III |
| 24 |
NC_000070.6 |
4 |
146604431 |
+ |
Gm13240 |
NNNCCATATGACACTAGCAA |
NGG |
2 |
0.1128 |
Tier III |
| 25 |
NC_000070.6 |
4 |
147443686 |
+ |
Gm13161 |
NNNCCATATGACACTAGCAA |
NGG |
2 |
0.1128 |
Tier III |
Other clones with same target sequence:
(none)
