Construct: sgRNA BRDN0001146481

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
AGCTGAACATAAGGCCACAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ROCK2 (9475)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76350

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000002.12 2 11211753 - ROCK2 NNNTGAACATAAGGCCACAA NGG 0 1.0 Tier I
2 NC_000020.11 20 5946667 + TRMT6 NNNTGAACATAAAGCAACAA NGG 2 0.9231 Tier II
3 NC_000002.12 2 46591994 + PIGF NNNTGAATATAAGACCACAA NGG 2 0.6563 Tier II
4 NC_000008.11 8 42703734 - CHRNB3 NNNTGAACAAAAGGCCATAA NGG 2 0.551 Tier II
5 NC_000007.14 7 44273744 - CAMK2B NNNTGGACATAAGGCCACAG NGG 2 0.5462 Tier II
6 NC_000017.11 17 66366435 - PRKCA NNNTGAAAATAAGACCACAA NGG 2 0.4875 Tier II
7 NC_000003.12 3 39071314 - WDR48 NNNTGAAAATGAGGCCACAA NGG 2 0.4225 Tier II
8 NC_000012.12 12 49653433 + FMNL3 NNNTCAACACAAGGCCACAA NGG 2 0.419 Tier II
9 NC_000010.11 10 123691016 + GPR26 NNNTGAAAATAAGGCCATAA NGG 2 0.4179 Tier II
10 NC_000007.14 7 152297152 + KMT2C NNNTGAACATCAGGCAACAA NGG 2 0.4 Tier II
11 NC_000008.11 8 50090900 + SNTG1 NNNTGAAATTAAGGCCACAA NGG 2 0.39 Tier II
12 NC_000003.12 3 113986689 + CCDC191 NNNTGAACATAGGGCCACTA NGG 2 0.3889 Tier II
13 NC_000004.12 4 83038459 - COPS4 NNNTGAAAATAAGGCCAAAA NGG 2 0.35 Tier II
14 NC_000004.12 4 2344035 + ZFYVE28 NNNTGGACATCAGGCCACAA NGG 2 0.2857 Tier II
15 NC_000002.12 2 17770555 - GEN1 NNNTTAACATAAAGCCACAA NGG 2 0.2769 Tier II
16 NC_000023.11 X 97356091 - DIAPH2 NNNTGAACATAATACCACAA NGG 2 0.225 Tier II
17 NC_000007.14 7 20714066 - ABCB5 NNNTGAACATAAGGCAACCA NGG 2 0.2069 Tier II
18 NC_000016.10 16 58494424 - NDRG4 NNNTGACCATCAGGCCACAA NGG 2 0.175 Tier II
19 NC_000007.14 7 122878787 + CADPS2 NNNTGAACATTAGCCCACAA NGG 2 0.1319 Tier II
20 NC_000011.10 11 63299297 - SLC22A10 NNNTGAAAATAAGGCCCCAA NGG 2 0.1147 Tier II
21 NC_000008.11 8 18019296 + PCM1 NNNTGGACATAAGGCCTCAA NGG 2 0.0952 Tier II
22 NC_000003.12 3 66342362 - SLC25A26 NNNTGAACATAATGCCACCA NGG 2 0.0621 Tier II
23 NC_000023.11 X 91894972 - PCDH11X NNNTGAACATAGGGTCACAA NGG 2 0.0481 Tier II
24 NC_000024.10 Y 5117083 - PCDH11Y NNNTGAACATAGGGTCACAA NGG 2 0.0481 Tier II
25 NC_000005.10 5 79303809 - JMY NNNTGAACATAAGGCCCCAA NAG 2 0.0458 Tier II
26 NC_000007.14 7 72479472 + CALN1 NNNTTAACATAAGGTCACAA NGG 2 0.02 Tier II
27 NC_000014.9 14 51599374 + FRMD6 NNNTAAACATAAGGCCACAA NGT 2 0.014 Tier II
28 NC_000015.10 15 22819229 - NIPA1 NNNTGAACCTAAGGCCACAA NGC 2 0.0127 Tier II
29 NC_000015.10 15 97262705 - LINC02253 NNNTGAACACAAAGCCACAA NGG 2 0.4923 Tier III
30 NC_000009.12 9 78360000 - LOC107987083 NNNTGAAAATAAGGCCAAAA NGG 2 0.35 Tier III
31 NC_000012.12 12 94045742 + LOC105369913 NNNTGAAAATAAGGCCAAAA NGG 2 0.35 Tier III
32 NC_000023.11 X 20616804 - LOC124905257 NNNTCAACATCAGGCCACAA NGG 2 0.3143 Tier III
33 NC_000020.11 20 25771957 + FAM182B NNNTGAACATCAGGCCACAG NGG 2 0.3059 Tier III
34 NC_000004.12 4 2344035 + LOC105374352 NNNTGGACATCAGGCCACAA NGG 2 0.2857 Tier III
35 NC_000002.12 2 17770555 - LOC105373449 NNNTTAACATAAAGCCACAA NGG 2 0.2769 Tier III
36 NC_000018.10 18 38697521 + LOC105372076 NNNTGATCATAAGGCCACAA NAG 2 0.1134 Tier III
37 NC_000004.12 4 36556268 - LINC02505 NNNTGAACACAAGGCCTCAA NGG 2 0.0711 Tier III
38 NC_000014.9 14 51599374 + FRMD6-AS2 NNNTAAACATAAGGCCACAA NGT 2 0.014 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000082.6 16 10294915 - Emp2 NNNTGAACATAAAGCCACAA NGG 1 0.9231 Tier II
2 NC_000082.6 16 95503108 + Erg NNNTAAACATAAGGCAACAA NGG 2 0.8667 Tier II
3 NC_000082.6 16 72842143 - Robo1 NNNTGAACAAAAGGCAACAA NGG 2 0.8571 Tier II
4 NC_000069.6 3 38961035 - Fat4 NNNTGAACAAAAGACCACAA NGG 2 0.6429 Tier II
5 NC_000069.6 3 115019209 - Olfm3 NNNTGAACAAAGGGCCACAA NGG 2 0.619 Tier II
6 NC_000081.6 15 4570351 + Plcxd3 NNNTGAACATAGGGCCACAG NGG 2 0.5523 Tier II
7 NC_000081.6 15 84452329 + Shisal1 NNNTGACCATAAGGCCACAA NGG 1 0.4375 Tier II
8 NC_000068.7 2 125781152 + Secisbp2l NNNTGAACATAAGCCAACAA NGG 2 0.4286 Tier II
9 NC_000070.6 4 119889003 - Hivep3 NNNTGAACAGAATGCCACAA NGG 2 0.15 Tier II
10 NC_000079.6 13 49561236 - Cenpp NNNTGAACTTAAGGACACAA NGG 2 0.1333 Tier II
11 NC_000079.6 13 49561236 - Aspn NNNTGAACTTAAGGACACAA NGG 2 0.1333 Tier II
12 NC_000076.6 10 127587683 - Lrp1 NNNTGAACGTAAGGCCAGAA NGG 2 0.0889 Tier II
13 NC_000070.6 4 86264514 - Adamtsl1 NNNTGAACATGAGGCCAGAA NGG 2 0.0867 Tier II
14 NC_000076.6 10 62408733 + Hkdc1 NNNTGAACAGAAGGCCTCAA NGG 2 0.0667 Tier II
15 NC_000073.6 7 133923291 + Adam12 NNNTGAACATAATGCCACCA NGG 2 0.0621 Tier II
16 NC_000073.6 7 13290514 + Lig1 NNNTGAACATTAGGCCAGAA NGG 2 0.041 Tier II
17 NC_000073.6 7 77108765 - Agbl1 NNNTTAACATAAGGCCAGAA NGG 2 0.04 Tier II
18 NC_000076.6 10 20496408 - Pde7b NNNTGAACATAAGGCTACAA NGA 2 0.0214 Tier II
19 NC_000077.6 11 36955200 - Tenm2 NNNTGAACATATGGGCACAA NGG 2 0.0167 Tier II
20 NC_000083.6 17 44827224 - Supt3 NNNTGAACATAAGGGCACAA NAG 2 0.013 Tier II
21 NC_000071.6 5 5456122 + Pttg1ip2 NNNTGAACATAAGGCCACAA NAA 2 0.0 Tier II
22 NC_000081.6 15 84452329 + Gm33830 NNNTGACCATAAGGCCACAA NGG 1 0.4375 Tier III
23 NC_000067.6 1 177290867 + Gm36904 NNNTGCACATAAGGACACAA NGG 2 0.101 Tier III
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Other clones with same target sequence:

(none)