Construct: sgRNA BRDN0001146498
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CCCCCACAGGACTTACCTAG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- ITPKA (3706)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76856
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000015.10 |
15 |
41502005 |
+ |
ITPKA |
NNNCCACAGGACTTACCTAG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000017.11 |
17 |
45772390 |
+ |
LINC02210-CRHR1 |
NNNTCATAGGACTTACCTAG |
NGG |
2 |
0.65 |
Tier II |
| 3 |
NC_000006.12 |
6 |
35689310 |
+ |
FKBP5 |
NNNCCACAGGGCCTACCTAG |
NGG |
2 |
0.5132 |
Tier II |
| 4 |
NC_000012.12 |
12 |
6050608 |
+ |
VWF |
NNNCCAGAGGACTTACCTTG |
NGG |
2 |
0.2534 |
Tier II |
| 5 |
NC_000022.11 |
22 |
32718843 |
+ |
SYN3 |
NNNCCACATGACTTACCTAC |
NGG |
2 |
0.2286 |
Tier II |
| 6 |
NC_000010.11 |
10 |
17768640 |
+ |
TMEM236 |
NNNCCACAGAACTTACCTCG |
NGG |
2 |
0.1931 |
Tier II |
| 7 |
NC_000020.11 |
20 |
23352835 |
+ |
NXT1 |
NNNCCACAGGACTTATCTGG |
NGG |
2 |
0.1154 |
Tier II |
| 8 |
NC_000006.12 |
6 |
62101420 |
+ |
KHDRBS2 |
NNNCCACAGGATTTACGTAG |
NGG |
2 |
0.0317 |
Tier II |
| 9 |
NC_000016.10 |
16 |
13284230 |
+ |
SHISA9 |
NNNTCACAGGACTTACCTAG |
NTG |
2 |
0.0312 |
Tier II |
| 10 |
NC_000001.11 |
1 |
14426367 |
+ |
KAZN |
NNNCCACAGGACTTACCTAT |
NTG |
2 |
0.0273 |
Tier II |
| 11 |
NC_000024.10 |
Y |
7048673 |
+ |
TBL1Y |
NNNCCCCAGGACTTACCTAG |
NTG |
2 |
0.0177 |
Tier II |
| 12 |
NC_000022.11 |
22 |
25085013 |
+ |
KIAA1671 |
NNNCCACAGGACTTACATAG |
NGT |
2 |
0.0075 |
Tier II |
| 13 |
NC_000017.11 |
17 |
14034009 |
+ |
COX10-DT |
NNNCCAAAGGACTCACCTAG |
NGG |
2 |
0.2143 |
Tier III |
| 14 |
NC_000017.11 |
17 |
72558512 |
- |
LINC00673 |
NNNCCACATGACTTTCCTAG |
NGG |
2 |
0.1067 |
Tier III |
| 15 |
NC_000016.10 |
16 |
13284230 |
+ |
LOC107984137 |
NNNTCACAGGACTTACCTAG |
NTG |
2 |
0.0312 |
Tier III |
| 16 |
NC_000014.9 |
14 |
53836633 |
+ |
LINC02331 |
NNNCCACAGGTCTTACCTAG |
NGA |
2 |
0.0214 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000077.6 |
11 |
73217919 |
+ |
Shpk |
NNNCGACAGGACCTACCTAG |
NGG |
2 |
0.4737 |
Tier I |
| 2 |
NC_000068.7 |
2 |
96867439 |
+ |
Lrrc4c |
NNNCCACAGGACTAACCTAG |
NGG |
1 |
0.619 |
Tier II |
| 3 |
NC_000073.6 |
7 |
56065513 |
+ |
Herc2 |
NNNCCTCAGGACCTACCTAG |
NGG |
2 |
0.5639 |
Tier II |
| 4 |
NC_000071.6 |
5 |
142111408 |
- |
Sdk1 |
NNNGCACAGGACTTACCTTG |
NGG |
2 |
0.2692 |
Tier II |
| 5 |
NC_000072.6 |
6 |
137783999 |
- |
Dera |
NNNCCACAGGACTCACATAG |
NGG |
2 |
0.1333 |
Tier II |
| 6 |
NC_000071.6 |
5 |
64805217 |
- |
Klf3 |
NNNCCACAGGACTTGGCTAG |
NGG |
2 |
0.1 |
Tier II |
| 7 |
NC_000071.6 |
5 |
64805217 |
- |
Gm38457 |
NNNCCACAGGACTTGGCTAG |
NGG |
2 |
0.1 |
Tier II |
| 8 |
NC_000084.6 |
18 |
75245201 |
- |
Dym |
NNNCCACAGGACTTCCCGAG |
NGG |
2 |
0.0909 |
Tier II |
| 9 |
NC_000083.6 |
17 |
74753906 |
- |
Ttc27 |
NNNCCACAGGTCTTTCCTAG |
NGG |
2 |
0.0615 |
Tier II |
| 10 |
NC_000072.6 |
6 |
76995891 |
+ |
Ctnna2 |
NNNCCACATGACTTACCTAG |
NGA |
2 |
0.037 |
Tier II |
| 11 |
NC_000084.6 |
18 |
39336043 |
- |
Arhgap26 |
NNNCCACAGGCCTTACCTAG |
NGA |
2 |
0.0278 |
Tier II |
| 12 |
NC_000070.6 |
4 |
117302986 |
- |
Rnf220 |
NNNCCAAAGGACTTACCTAG |
NGT |
2 |
0.0121 |
Tier II |
| 13 |
NC_000083.6 |
17 |
13254703 |
+ |
Gm36684 |
NNNACACAGGATTTACCTAG |
NGG |
2 |
0.4534 |
Tier III |
| 14 |
NC_000083.6 |
17 |
13538886 |
+ |
2700054A10Rik |
NNNACACAGGATTTACCTAG |
NGG |
2 |
0.4534 |
Tier III |
| 15 |
NC_000070.6 |
4 |
117302986 |
- |
Gm12843 |
NNNCCAAAGGACTTACCTAG |
NGT |
2 |
0.0121 |
Tier III |
Other clones with same target sequence:
(none)
