Construct: sgRNA BRDN0001146528
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TTAACATAGACAAATACGAC
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- CERK (64781)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76332
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000022.11 |
22 |
46711091 |
- |
CERK |
NNNACATAGACAAATACGAC |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000023.11 |
X |
37373144 |
- |
PRRG1 |
NNNACATAGACAAATACAAC |
NGG |
1 |
0.6923 |
Tier II |
| 3 |
NC_000014.9 |
14 |
32082888 |
- |
ARHGAP5 |
NNNAGATAGACAAATACTAC |
NGG |
2 |
0.4 |
Tier II |
| 4 |
NC_000012.12 |
12 |
78088288 |
+ |
NAV3 |
NNNACATAGACAAATATAAC |
NGG |
2 |
0.3231 |
Tier II |
| 5 |
NC_000008.11 |
8 |
14780092 |
+ |
SGCZ |
NNNACATAGAAAAATACGGC |
NGG |
2 |
0.2813 |
Tier II |
| 6 |
NC_000007.14 |
7 |
83008806 |
+ |
PCLO |
NNNACATAGACAAAAATGAC |
NGG |
2 |
0.2702 |
Tier II |
| 7 |
NC_000003.12 |
3 |
9049234 |
+ |
SRGAP3 |
NNNACATACACAAATAAGAC |
NGG |
2 |
0.2513 |
Tier II |
| 8 |
NC_000022.11 |
22 |
24998009 |
- |
KIAA1671 |
NNNACATGGATAAATACGAC |
NGG |
2 |
0.2256 |
Tier II |
| 9 |
NC_000011.10 |
11 |
107008650 |
+ |
GUCY1A2 |
NNNACATAGATAAATAAGAC |
NGG |
2 |
0.1436 |
Tier II |
| 10 |
NC_000005.10 |
5 |
147880785 |
- |
SCGB3A2 |
NNNACATAGACAAATAAGAC |
NAG |
2 |
0.121 |
Tier II |
| 11 |
NC_000004.12 |
4 |
103034122 |
+ |
SLC9B2 |
NNNACATAGACAAATAGAAC |
NGG |
2 |
0.0407 |
Tier II |
| 12 |
NC_000010.11 |
10 |
85767535 |
+ |
GRID1 |
NNNAGATAGACAAATACGAC |
NTG |
2 |
0.0234 |
Tier II |
| 13 |
NC_000002.12 |
2 |
143018484 |
- |
KYNU |
NNNACATAGACCAATAGGAC |
NGG |
2 |
0.0155 |
Tier II |
| 14 |
NC_000011.10 |
11 |
86814903 |
- |
PRSS23 |
NNNACATAGACAAATACCAC |
NGT |
2 |
0.0077 |
Tier II |
| 15 |
NC_000006.12 |
6 |
161659443 |
- |
PRKN |
NNNACATAGACAAATAGGAC |
NGT |
2 |
0.0009 |
Tier II |
| 16 |
NC_000020.11 |
20 |
8098001 |
+ |
PHKBP1 |
NNNACATTGTCAAATACGAC |
NGG |
2 |
0.3782 |
Tier III |
| 17 |
NC_000002.12 |
2 |
14230160 |
- |
LINC00276 |
NNNACATAGACTAATACAAC |
NGG |
2 |
0.2308 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000079.6 |
13 |
29761878 |
+ |
Cdkal1 |
NNNGCAAAGACAAATACGAC |
NGG |
2 |
0.5469 |
Tier II |
| 2 |
NC_000068.7 |
2 |
22557465 |
- |
Myo3a |
NNNACATAGACAAATACAGC |
NGG |
2 |
0.2596 |
Tier II |
| 3 |
NC_000075.6 |
9 |
120157320 |
- |
Mobp |
NNNACATAGACAAATACACC |
NGG |
2 |
0.1432 |
Tier II |
| 4 |
NC_000086.7 |
X |
83603009 |
+ |
Dmd |
NNNACATAGACATATAAGAC |
NGG |
2 |
0.14 |
Tier II |
| 5 |
NC_000071.6 |
5 |
16276620 |
- |
Cacna2d1 |
NNNACATAGACAAACACCAC |
NGG |
2 |
0.1299 |
Tier II |
| 6 |
NC_000072.6 |
6 |
101201310 |
- |
Pdzrn3 |
NNNACATTGACAAACACGAC |
NGG |
2 |
0.1169 |
Tier II |
| 7 |
NC_000071.6 |
5 |
129194915 |
+ |
Adgrd1 |
NNNACATAGACACATACCAC |
NGG |
2 |
0.1003 |
Tier II |
| 8 |
NC_000081.6 |
15 |
53889731 |
- |
Samd12 |
NNNACATAGACAAAGAAGAC |
NGG |
2 |
0.0233 |
Tier II |
| 9 |
NC_000073.6 |
7 |
134491805 |
+ |
D7Ertd443e |
NNNACATAGACAAATACGAC |
NGC |
1 |
0.0222 |
Tier II |
| 10 |
NC_000077.6 |
11 |
118065769 |
+ |
Dnah17 |
NNNACATAGACAGATACGAC |
NGT |
2 |
0.0105 |
Tier II |
| 11 |
NC_000067.6 |
1 |
51577721 |
+ |
Gm17767 |
NNNACATAGAAAAATACAAC |
NGG |
2 |
0.5192 |
Tier III |
| 12 |
NC_000072.6 |
6 |
101201310 |
- |
Gm26911 |
NNNACATTGACAAACACGAC |
NGG |
2 |
0.1169 |
Tier III |
Other clones with same target sequence:
(none)
