Construct: sgRNA BRDN0001146562

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
TGGACAATGAAGACACTCTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PSKH2 (85481)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77267

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000008.11 8 86064152 - PSKH2 NNNACAATGAAGACACTCTG NGG 0 1.0 Tier I
2 NC_000001.11 1 243563751 - AKT3 NNNACCATGAAGACATTCTG NGG 2 0.1399 Tier I
3 NC_000010.11 10 62229524 + RTKN2 NNNACAATGAAGACACTTTT NGG 2 0.45 Tier II
4 NC_000002.12 2 134483109 + TMEM163 NNNACAATGAGGGCACTCTG NGG 2 0.4239 Tier II
5 NC_000001.11 1 33550724 - CSMD2 NNNCCAATGAAGACAATCTG NGG 2 0.3529 Tier II
6 NC_000003.12 3 100550032 + TMEM45A NNNACAATAAACACACTCTG NGG 2 0.3403 Tier II
7 NC_000011.10 11 22758271 - GAS2 NNNGCAATGAAGACACTATG NGG 2 0.3365 Tier II
8 NC_000010.11 10 7218628 + SFMBT2 NNNCCAATGAAAACACTCTG NGG 2 0.3294 Tier II
9 NC_000003.12 3 66018885 - MAGI1 NNNACAATGGAGACACACTG NGG 2 0.2963 Tier II
10 NC_000007.14 7 127156095 + GRM8 NNNACTATGAAGAAACTCTG NGG 2 0.25 Tier II
11 NC_000011.10 11 10103067 - SBF2 NNNCCAATGAAGACACCCTG NGG 2 0.2491 Tier II
12 NC_000010.11 10 106716724 - SORCS1 NNNTCAATGAAGACATTCTG NGG 2 0.1958 Tier II
13 NC_000010.11 10 122050811 - TACC2 NNNACAATGAAGATACTCTG NAG 2 0.1901 Tier II
14 NC_000023.11 X 110079817 + TMEM164 NNNAAAATGATGACACTCTG NGG 2 0.1758 Tier II
15 NC_000007.14 7 43161828 - HECW1 NNNACAATGAAGACACTTTG NAG 2 0.1667 Tier II
16 NC_000012.12 12 66218773 - IRAK3 NNNACAATGAACACATTCTG NGG 2 0.1629 Tier II
17 NC_000015.10 15 47412953 + SEMA6D NNNAAAATGAAGACTCTCTG NGG 2 0.1143 Tier II
18 NC_000015.10 15 40979719 + INO80 NNNCCAATGATGACACTCTG NGG 2 0.1086 Tier II
19 NC_000002.12 2 157269465 - GALNT5 NNNACAATGAAGAAATTCTG NGG 2 0.1077 Tier II
20 NC_000005.10 5 119315946 - TNFAIP8 NNNACAATGAAGAAACTCTG NAG 2 0.0907 Tier II
21 NC_000006.12 6 39905503 - MOCS1 NNNAGAATGAAGACACTGTG NGG 2 0.08 Tier II
22 NC_000007.14 7 114542562 - FOXP2 NNNACAATGAAGCCACTCTG NAG 2 0.0546 Tier II
23 NC_000002.12 2 19695212 + LOC124905977 NNNACAAGGAAGACACTCTG NGA 2 0.0509 Tier II
24 NC_000008.11 8 17770621 + MTUS1 NNNACAATGAAGCCTCTCTG NGG 2 0.0421 Tier II
25 NC_000018.10 18 34826498 - DTNA NNNACAATGAAGACATTCTG NGA 2 0.0214 Tier II
26 NC_000002.12 2 77470511 + LRRTM4 NNNACAATGAAGACACACTG NGC 2 0.0119 Tier II
27 NC_000011.10 11 73538769 - FAM168A NNNACAATGAAGACACTCTT NGT 2 0.0113 Tier II
28 NC_000006.12 6 140022425 - LINC02941 NNNAGAATGAAGACAATCTG NGG 2 0.6 Tier III
29 NC_000011.10 11 15634789 + LINC02751 NNNAAAATGAAGACACTCTG NGG 1 0.5714 Tier III
30 NC_000022.11 22 29713056 + CABP7-DT NNNAAAATGAAGACACACTG NGG 2 0.3048 Tier III
31 NC_000003.12 3 66018885 - LOC124900543 NNNACAATGGAGACACACTG NGG 2 0.2963 Tier III
32 NC_000007.14 7 43161828 - HECW1-IT1 NNNACAATGAAGACACTTTG NAG 2 0.1667 Tier III
33 NC_000006.12 6 132920467 - LOC105378008 NNNACAAAGAAGACTCTCTG NGG 2 0.16 Tier III
34 NC_000001.11 1 117641398 - VDAC2P3 NNNACACTGATGACACTCTG NGG 2 0.1346 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000071.6 5 35706641 + Sh3tc1 NNNACAGTGAGGACACTCTG NGG 2 0.4588 Tier I
2 NC_000078.6 12 112657099 - Akt1 NNNACTATGAAGACATTCTG NGG 2 0.2198 Tier I
3 NC_000067.6 1 177059365 - Akt3 NNNACCATGAAGACATTCTG NGG 2 0.1399 Tier I
4 NC_000076.6 10 128254833 + Apon NNNACAATGAAGTCATTCTG NGG 2 0.0923 Tier I
5 NC_000075.6 9 118519287 - Golga4 NNNACACTGAAGACACTCTG NGG 1 0.4375 Tier II
6 NC_000073.6 7 51584469 + Ano5 NNNACAAAGAAGACACACTG NGG 2 0.4267 Tier II
7 NC_000084.6 18 65216667 - Nedd4l NNNACACTGAAAACACTCTG NGG 2 0.4083 Tier II
8 NC_000067.6 1 32511623 + Khdrbs2 NNNACAATGAAGAAAATCTG NGG 2 0.35 Tier II
9 NC_000068.7 2 60853649 - Rbms1 NNNACAATAAAGACACACTG NGG 2 0.3429 Tier II
10 NC_000077.6 11 49085702 - Ifi47 NNNACCATGAAGATACTCTG NGG 2 0.3333 Tier II
11 NC_000068.7 2 132085930 + Slc23a2 NNNACAAGGAAGACACTCTC NGG 2 0.3143 Tier II
12 NC_000071.6 5 117345532 + Vsig10 NNNACTCTGAAGACACTCTG NGG 2 0.3125 Tier II
13 NC_000080.6 14 124440309 - Fgf14 NNNACTTTGAAGACACTCTG NGG 2 0.3125 Tier II
14 NC_000078.6 12 53842108 + Npas3 NNNAAAATTAAGACACTCTG NGG 2 0.3048 Tier II
15 NC_000072.6 6 106623973 + Cntn4 NNNACACTGAAGGCACTCTG NGG 2 0.2853 Tier II
16 NC_000068.7 2 27932747 + Col5a1 NNNAGCATGAAGACACTCTG NGG 2 0.2727 Tier II
17 NC_000073.6 7 87673799 + Grm5 NNNACCATGAAGACACACTG NGG 2 0.2424 Tier II
18 NC_000080.6 14 25511641 - Zmiz1 NNNAAAATGAATACACTCTG NGG 2 0.2198 Tier II
19 NC_000071.6 5 38811538 + Clnk NNNTCAATGAAGTCACTCTG NGG 2 0.1909 Tier II
20 NC_000082.6 16 34722371 - Ccdc14 NNNAGAATGAAGACATTCTG NGG 2 0.1846 Tier II
21 NC_000070.6 4 104458171 + Dab1 NNNAGAATGAAGACACTCTG NAG 2 0.1556 Tier II
22 NC_000068.7 2 156267059 - Phf20 NNNACAATGAAGACACTAAG NGG 2 0.1538 Tier II
23 NC_000069.6 3 28509621 - Tnik NNNACAATGAAGAAACTCTC NGG 2 0.15 Tier II
24 NC_000084.6 18 39096770 - Arhgap26 NNNATAATGAAGACTCTCTG NGG 2 0.1273 Tier II
25 NC_000076.6 10 39104814 - Lama4 NNNACACTGAAGACACTCCG NGG 2 0.1207 Tier II
26 NC_000077.6 11 55590734 - Glra1 NNNACAAAGAAGACACTGTG NGG 2 0.1067 Tier II
27 NC_000068.7 2 118218560 + Fsip1 NNNACAATGAAGACTCACTG NGG 2 0.1067 Tier II
28 NC_000074.6 8 12852742 + Atp11a NNNACAATGCAGACATTCTG NGG 2 0.1026 Tier II
29 NC_000070.6 4 84289017 + Bnc2 NNNACAAGGAAGACACTGTG NGG 2 0.0978 Tier II
30 NC_000080.6 14 10908575 + Fhit NNNACAATGAAGGCACTGTG NGG 2 0.087 Tier II
31 NC_000078.6 12 91374863 - Cep128 NNNACAATGAAGACAATCTG NTG 2 0.039 Tier II
32 NC_000074.6 8 122147119 - Zfp469 NNNACCATGAAGACACTCTG NGA 2 0.0316 Tier II
33 NC_000075.6 9 97662699 + Clstn2 NNNACAATGAAGACACTTTG NGC 2 0.0143 Tier II
34 NC_000067.6 1 50944901 - Tmeff2 NNNACAATGAAGATACTCTG NGT 2 0.0118 Tier II
35 NC_000073.6 7 66782685 + Cers3 NNNACAGTGAAGACACTCTG NGT 2 0.0114 Tier II
36 NC_000068.7 2 106786479 + Mpped2 NNNACAAAGAAGAGACTCTG NGG 2 0.0 Tier II
37 NC_000070.6 4 104684934 + Dab1 NNNACAATGAAGAGGCTCTG NGG 2 0.0 Tier II
38 NC_000068.7 2 120390972 + Tmem87a NNNACAATGAAGAGTCTCTG NGG 2 0.0 Tier II
39 NC_000079.6 13 9430282 - Dip2c NNNACAATGCAGAGACTCTG NGG 2 0.0 Tier II
40 NC_000076.6 10 17562310 - Gm32543 NNNACAGTGAAGACAATCTG NGG 2 0.7059 Tier III
41 NC_000070.6 4 109269080 + Gm40225 NNNACAATCAAGACAATCTG NGG 2 0.5385 Tier III
42 NC_000078.6 12 71358629 + Gm40438 NNNACAATAAAGACACTATG NGG 2 0.3462 Tier III
43 NC_000078.6 12 53842108 + Gm35614 NNNAAAATTAAGACACTCTG NGG 2 0.3048 Tier III
44 NC_000080.6 14 13204780 - Gm5087 NNNACAATGAAAACAGTCTG NGG 2 0.1436 Tier III
45 NC_000078.6 12 15659561 - Gm40287 NNNACGATGAAGACACTGTG NGG 2 0.0952 Tier III
46 NC_000075.6 9 25219539 + Gm29642 NNNACAATCAAGACACTGTG NGG 2 0.0718 Tier III
47 NC_000074.6 8 122147119 - Gm39268 NNNACCATGAAGACACTCTG NGA 2 0.0316 Tier III
48 NC_000077.6 11 54273464 - LOC115487722 NNNACAATGAAGAGACTGTG NGG 2 0.0 Tier III
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Other clones with same target sequence:

(none)