Construct: sgRNA BRDN0001146567
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GCTGAAATTGCTAGTGCATT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- C8orf44-SGK3 (100533105)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75906
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000008.11 |
8 |
66840080 |
+ |
SGK3 |
NNNGAAATTGCTAGTGCATT |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000008.11 |
8 |
66840080 |
+ |
C8orf44-SGK3 |
NNNGAAATTGCTAGTGCATT |
NGG |
0 |
1.0 |
Tier I |
| 3 |
NC_000015.10 |
15 |
36656106 |
+ |
CDIN1 |
NNNGAAATTGATAATGCATT |
NGG |
2 |
0.5625 |
Tier II |
| 4 |
NC_000011.10 |
11 |
5502534 |
- |
OR51B5 |
NNNGAAATTGCTAGTGCATA |
NGG |
1 |
0.5625 |
Tier II |
| 5 |
NC_000007.14 |
7 |
34731437 |
+ |
NPSR1 |
NNNGAAATTGGTAGTGCATT |
NGG |
1 |
0.25 |
Tier II |
| 6 |
NC_000012.12 |
12 |
107357093 |
+ |
ABTB3 |
NNNGAAGTTGCTTGTGCATT |
NGG |
2 |
0.2118 |
Tier II |
| 7 |
NC_000016.10 |
16 |
72111215 |
+ |
DHX38 |
NNNGAAGTTGCTTGTGCATT |
NGG |
2 |
0.2118 |
Tier II |
| 8 |
NC_000008.11 |
8 |
102267347 |
- |
UBR5 |
NNNGAATTTGCTAGTGAATT |
NGG |
2 |
0.2042 |
Tier II |
| 9 |
NC_000003.12 |
3 |
2741940 |
- |
CNTN4 |
NNNTAAATTGCTAGTGCATT |
NGA |
2 |
0.0253 |
Tier II |
| 10 |
NC_000007.14 |
7 |
111959921 |
- |
DOCK4 |
NNNGAAATTGCTAGTGCATC |
NAG |
2 |
0.0236 |
Tier II |
| 11 |
NC_000005.10 |
5 |
81158384 |
+ |
RASGRF2 |
NNNGAAATTGTTAGTGGATT |
NGG |
2 |
0.0181 |
Tier II |
| 12 |
NC_000008.11 |
8 |
96689515 |
- |
CPQ |
NNNGAAATTGCTAGTGCATT |
NAC |
2 |
0.0 |
Tier II |
| 13 |
NC_000008.11 |
8 |
86479569 |
+ |
RMDN1 |
NNNGAAATTGCTATTTCATT |
NGG |
2 |
0.0 |
Tier II |
| 14 |
NC_000007.14 |
7 |
34731437 |
+ |
NPSR1-AS1 |
NNNGAAATTGGTAGTGCATT |
NGG |
1 |
0.25 |
Tier III |
| 15 |
NC_000001.11 |
1 |
204613966 |
+ |
LOC105371692 |
NNNGAATTTGCTAGTGCACT |
NGG |
2 |
0.1207 |
Tier III |
| 16 |
NC_000007.14 |
7 |
26061230 |
- |
LOC105375199 |
NNNGAAATCGCTAGTGCATT |
NGA |
2 |
0.0641 |
Tier III |
| 17 |
NC_000001.11 |
1 |
189363941 |
+ |
LOC105371657 |
NNNGAAATTGTTAGTGCATT |
NTG |
2 |
0.012 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000076.6 |
10 |
4156204 |
+ |
Mthfd1l |
NNNGAGATTGCAAGTGCATT |
NGG |
2 |
0.5714 |
Tier II |
| 2 |
NC_000067.6 |
1 |
34784592 |
+ |
Arhgef4 |
NNNGAAATTCCTGGTGCATT |
NGG |
2 |
0.2609 |
Tier II |
| 3 |
NC_000086.7 |
X |
168322531 |
- |
Frmpd4 |
NNNGAAACTGCTAGTGCATT |
NAG |
2 |
0.2593 |
Tier II |
| 4 |
NC_000076.6 |
10 |
27325423 |
- |
Lama2 |
NNNTAAATTGCTAGTGCAAT |
NGG |
2 |
0.1039 |
Tier II |
| 5 |
NC_000079.6 |
13 |
54465328 |
+ |
Thoc3 |
NNNGAAATTGCTAGTGCAAT |
NAG |
2 |
0.0741 |
Tier II |
| 6 |
NC_000081.6 |
15 |
21202896 |
+ |
Cdh12 |
NNNGAGATTGCTAGTGCATT |
NGT |
2 |
0.0115 |
Tier II |
| 7 |
NC_000085.6 |
19 |
48537902 |
- |
Sorcs3 |
NNNGAAATTGCTAGTGCATT |
NCA |
2 |
0.0 |
Tier II |
| 8 |
NC_000086.7 |
X |
76198154 |
+ |
Gm5397 |
NNNGAAATTGAGAGTGCATT |
NGG |
2 |
0.375 |
Tier III |
| 9 |
NC_000067.6 |
1 |
157077696 |
+ |
Gm30568 |
NNNGAAATTGCTGGTGAATT |
NGG |
2 |
0.3043 |
Tier III |
| 10 |
NC_000067.6 |
1 |
34784592 |
+ |
Gm32790 |
NNNGAAATTCCTGGTGCATT |
NGG |
2 |
0.2609 |
Tier III |
| 11 |
NC_000077.6 |
11 |
24515760 |
- |
4933430M04Rik |
NNNGAAATGGCTAGTGCATC |
NGG |
2 |
0.0563 |
Tier III |
Other clones with same target sequence:
(none)
