Construct: sgRNA BRDN0001146570

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
GTGGAGCCTCTTACACCCAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
EGFR (1956)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
75688

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000007.14 7 55173941 + EGFR NNNGAGCCTCTTACACCCAG NGG 0 1.0 Tier I
2 NC_000011.10 11 18728348 + PTPN5 NNNGAGCATCTGACACCCAG NGG 2 0.325 Tier I
3 NC_000012.12 12 56996418 + GPR182 NNNCAGCCTCTTACACCCAG NTG 2 0.0206 Tier I
4 NC_000022.11 22 41952227 - SMIM45 NNNGAGCTTCTCACACCCAG NGG 2 0.8289 Tier II
5 NC_000004.12 4 100458929 - EMCN NNNGAGCCTCTAGCACCCAG NGG 2 0.5217 Tier II
6 NC_000008.11 8 142220507 + TSNARE1 NNNGAGCCTCTCACACCCTG NGG 2 0.5101 Tier II
7 NC_000020.11 20 64181942 - MYT1 NNNGAGCCTCTGACACCCAA NGG 2 0.4688 Tier II
8 NC_000015.10 15 76086362 - TMEM266 NNNAAGCCTCTGACACCCAG NGG 2 0.45 Tier II
9 NC_000013.11 13 42991128 - EPSTI1 NNNTAGCCTCTTACAACCAG NGG 2 0.3636 Tier II
10 NC_000015.10 15 78973659 + RASGRF1 NNNGGGCCTCTTACACCCAC NGG 2 0.3086 Tier II
11 NC_000005.10 5 172127619 + STK10 NNNGAGGCTCTGACACCCAG NGG 2 0.2353 Tier II
12 NC_000009.12 9 133961890 + VAV2 NNNGAGCCTCATCCACCCAG NGG 2 0.1579 Tier II
13 NC_000015.10 15 41512521 - LTK NNNGAACCTCTTACACCGAG NGG 2 0.1333 Tier II
14 NC_000009.12 9 17448503 - CNTLN NNNGAGCCTCTTCCACCAAG NGG 2 0.1134 Tier II
15 NC_000011.10 11 78810856 + TENM4 NNNGAGCCTCTTCCACCCAC NGG 2 0.0902 Tier II
16 NC_000017.11 17 81050564 + BAIAP2 NNNGAGCCTCTTCCACCCGG NGG 2 0.0789 Tier II
17 NC_000009.12 9 124684670 + NR6A1 NNNGAGCCTCTTCCATCCAG NGG 2 0.0648 Tier II
18 NC_000017.11 17 1112102 + ABR NNNGAGCCTCTTACACCCAC NCG 2 0.0459 Tier II
19 NC_000012.12 12 96622012 + CFAP54 NNNGAGCTTCTTACACCCAG NTG 2 0.0341 Tier II
20 NC_000007.14 7 139732395 + HIPK2 NNNGAGCCACTTACACCCAG NGC 2 0.0206 Tier II
21 NC_000009.12 9 33104390 - B4GALT1 NNNGAGCCTCTTACTCCCAG NGT 2 0.0032 Tier II
22 NC_000020.11 20 2876193 + PTPRA NNNCAGCCTCTTAGACCCAG NGG 2 0.0 Tier II
23 NC_000004.12 4 100458929 - LOC124900740 NNNGAGCCTCTAGCACCCAG NGG 2 0.5217 Tier III
24 NC_000009.12 9 134491289 - LOC100506532 NNNGAGCCCCTGACACCCAG NGG 2 0.4615 Tier III
25 NC_000011.10 11 18728348 + IGSF22-AS1 NNNGAGCATCTGACACCCAG NGG 2 0.325 Tier III
26 NC_000002.12 2 216554557 - LOC101928180 NNNGAGGCTCTTACACACAG NGG 2 0.2196 Tier III
27 NC_000017.11 17 43674412 - LINC02594 NNNGAGCCTCTTACACACAC NGG 2 0.2 Tier III
28 NC_000004.12 4 109432349 + SEC24B-AS1 NNNGAGCCTCTTATACCCAG NAG 2 0.1901 Tier III
29 NC_000011.10 11 50186498 + LOC646797 NNNGAGACTCTTACAGCCAG NGG 2 0.1154 Tier III
30 NC_000011.10 11 50186498 + LOC124902811 NNNGAGACTCTTACAGCCAG NGG 2 0.1154 Tier III
31 NC_000009.12 9 17448503 - SAMM50P1 NNNGAGCCTCTTCCACCAAG NGG 2 0.1134 Tier III
32 NC_000009.12 9 124684670 + MIR181A2HG NNNGAGCCTCTTCCATCCAG NGG 2 0.0648 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000077.6 11 16891198 + Egfr NNNGAACCTCTCACACCCAG NGG 2 0.9474 Tier I
2 NC_000072.6 6 46653656 - Cntnap2 NNNGAGCCTTTTACACACAG NGG 2 0.4392 Tier II
3 NC_000079.6 13 77142145 - 2210408I21Rik NNNGAGTCTCTGACACCCAG NGG 2 0.4063 Tier II
4 NC_000068.7 2 127257326 + Tmem127 NNNGCTCCTCTTACACCCAG NGG 2 0.3333 Tier II
5 NC_000081.6 15 78541677 - Sstr3 NNNGAGCCTCCTACATCCAG NGG 2 0.2051 Tier II
6 NC_000075.6 9 29850835 - Ntm NNNGAGCATCTTACCCCCAG NGG 2 0.1773 Tier II
7 NC_000073.6 7 74296098 + Slco3a1 NNNGTGCCTCTTACTCCCAG NGG 2 0.0727 Tier II
8 NC_000083.6 17 68748413 + L3mbtl4 NNNGAGCCTCTTACAAGCAG NGG 2 0.0588 Tier II
9 NC_000085.6 19 31347838 + Prkg1 NNNGAGTCTCTTACACGCAG NGG 2 0.0478 Tier II
10 NC_000067.6 1 119526470 + Tmem185b NNNGAGCCTCTAACACGCAG NGG 2 0.0471 Tier II
11 NC_000074.6 8 71625499 + Unc13a NNNGAGCCTCTTATACCCAG NTG 2 0.0286 Tier II
12 NC_000078.6 12 4206876 + Cenpo NNNCAGCCTCTTACACCCAG NTG 2 0.0206 Tier II
13 NC_000078.6 12 4206876 + Adcy3 NNNCAGCCTCTTACACCCAG NTG 2 0.0206 Tier II
14 NC_000082.6 16 21280869 + Gm16863 NNNGAGCCTCTTACACCAAG NGG 1 0.5385 Tier III
15 NC_000079.6 13 50200838 + Gm38453 NNNGAGCCTCTGACACCCAG NAG 2 0.1296 Tier III
16 NC_000079.6 13 50610344 - Gm31890 NNNGAGCCTCTGACACCCAG NAG 2 0.1296 Tier III
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Other clones with same target sequence:

(none)