Construct: sgRNA BRDN0001146581

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
CCTTCTGCTTTACAAACAGG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
LATS1 (9113)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76024

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000006.12 6 149683917 - LATS1 NNNTCTGCTTTACAAACAGG NGG 0 1.0 Tier I
2 NC_000016.10 16 23480717 - GGA2 NNNTCTGCTGTACAAACAGG NGA 2 0.0347 Tier I
3 NC_000008.11 8 132881471 - TG NNNTCTGCTTTACAAACATA NGG 2 0.625 Tier II
4 NC_000002.12 2 87860188 + RGPD2 NNNGCTGTTTTACAAACAGG NGG 2 0.5469 Tier II
5 NC_000002.12 2 96733913 + LMAN2L NNNTCTGCTTTACAAACAAT NGG 2 0.5 Tier II
6 NC_000004.12 4 139545546 + SETD7 NNNTCTGCTTTACAAAAAGG NGG 1 0.4667 Tier II
7 NC_000006.12 6 24924098 + RIPOR2 NNNTCTGCTTTACAGACATG NGG 2 0.4333 Tier II
8 NC_000008.11 8 14666489 + SGCZ NNNTCTGCTTAACAAACAGC NGG 2 0.3214 Tier II
9 NC_000017.11 17 74219908 - TTYH2 NNNTCTGCTTTACAGAAAGG NGG 2 0.3033 Tier II
10 NC_000010.11 10 81998233 + NRG3 NNNTCTGCTTATCAAACAGG NGG 2 0.25 Tier II
11 NC_000012.12 12 86602837 - MGAT4C NNNTCTGCTTTACATACAGA NGG 2 0.1875 Tier II
12 NC_000008.11 8 130143937 + ASAP1 NNNTCTGCTTTACAAACAAG NAG 2 0.1852 Tier II
13 NC_000010.11 10 316399 + DIP2C NNNTCTGCTTTCCAAACAGT NGG 2 0.1842 Tier II
14 NC_000011.10 11 17376758 - NCR3LG1 NNNTCTGCTTTTAAAACAGG NGG 2 0.1282 Tier II
15 NC_000004.12 4 70998812 - DCK NNNTCGGCTTTACAAGCAGG NGG 2 0.1099 Tier II
16 NC_000015.10 15 64919922 - ANKDD1A NNNTTTGCTTTAGAAACAGG NGG 2 0.0868 Tier II
17 NC_000003.12 3 165775773 + BCHE NNNTCTGCTTTAGAAATAGG NGG 2 0.0636 Tier II
18 NC_000013.11 13 51097632 + C13orf42 NNNTCTACTTTACAAAGAGG NGG 2 0.0588 Tier II
19 NC_000023.11 X 18668551 + RS1 NNNTCTGCTTGAGAAACAGG NGG 2 0.0545 Tier II
20 NC_000005.10 5 137732912 - KLHL3 NNNTCTGCTTTACAAACAGT NTG 2 0.0273 Tier II
21 NC_000005.10 5 141674576 + ARAP3 NNNTCTGCTTTTCAAACAGG NGA 2 0.0231 Tier II
22 NC_000001.11 1 7222032 + CAMTA1 NNNTCTGCTTTCCAAACAGG NGC 2 0.0058 Tier II
23 NC_000012.12 12 80674040 + PTPRQ NNNTCTGCTTTACAAACAGG NAA 2 0.0 Tier II
24 NC_000007.14 7 19309387 - LOC107986773 NNNTCTGTTTTACAAACATG NGG 2 0.5833 Tier III
25 NC_000003.12 3 34250518 + LINC01811 NNNTCTGCTTTGCAAACAGG NAG 2 0.1872 Tier III
26 NC_000011.10 11 67389744 - LOC100130987 NNNTCTGCTTCCCAAACAGG NGG 2 0.1754 Tier III
27 NC_000016.10 16 89978142 - AFG3L1P NNNTCTGCTTTCCAAACATG NGG 2 0.1754 Tier III
28 NC_000007.14 7 65833071 - LOC124901659 NNNTCTGCTTTCCAAACAGC NGG 2 0.1128 Tier III
29 NC_000008.11 8 117496281 - LOC105375716 NNNTCTGCTTTACACACAGG NAG 2 0.0707 Tier III
30 NC_000009.12 9 124007327 + LHX2-AS1 NNNTCTGCCTTACAAACAGG NGA 2 0.0641 Tier III
31 NC_000007.14 7 140977221 + LOC105375536 NNNTCTGCTTTATAAACAGG NTG 2 0.0273 Tier III
32 NC_000012.12 12 80674040 + LOC105369867 NNNTCTGCTTTACAAACAGG NAA 2 0.0 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000085.6 19 59276510 + Slc18a2 NNNTCTGCTTTGCAAACATG NGG 2 0.4815 Tier I
2 NC_000073.6 7 121998930 - Gga2 NNNTCGGCTGTACAAACAGG NGG 2 0.2857 Tier I
3 NC_000085.6 19 41651701 - Slit1 NNNTCTGCTTTACAGACAGC NGG 2 0.2786 Tier I
4 NC_000076.6 10 7702283 + Lats1 NNNTCTGCTTTACAAACAGG NGC 1 0.0222 Tier I
5 NC_000071.6 5 112996399 - Grk3 NNNTCTGCTTCACAAACAGG NGG 1 0.6667 Tier II
6 NC_000068.7 2 30406847 + Crat NNNCCTGTTTTACAAACAGG NGG 2 0.5662 Tier II
7 NC_000070.6 4 70227437 - Cdk5rap2 NNNTCTGCTTTACAAACTGA NGG 2 0.4688 Tier II
8 NC_000068.7 2 170027036 - Tshz2 NNNTCTACTTTACAAATAGG NGG 2 0.4667 Tier II
9 NC_000068.7 2 52725711 - Stam2 NNNTCTTCTTTGCAAACAGG NGG 2 0.4127 Tier II
10 NC_000080.6 14 11839225 - Ptprg NNNTGTGCTTTACAAACATG NGG 2 0.4 Tier II
11 NC_000079.6 13 92849712 + Mtx3 NNNTGTGCTTTACAGACAGG NGG 2 0.39 Tier II
12 NC_000072.6 6 106066515 - Cntn4 NNNTCTGCTGTACAAACATG NGG 2 0.3333 Tier II
13 NC_000076.6 10 56123187 + Tbc1d32 NNNTCTGCTGTACAAACATG NGG 2 0.3333 Tier II
14 NC_000071.6 5 66809641 + Limch1 NNNCCTGCTGTACAAACAGG NGG 2 0.3235 Tier II
15 NC_000078.6 12 69600711 + Sos2 NNNCCTGCTGTACAAACAGG NGG 2 0.3235 Tier II
16 NC_000067.6 1 171417157 + Usf1 NNNGCTGCTTTACAAACTGG NGG 2 0.3125 Tier II
17 NC_000068.7 2 124417097 - Sema6d NNNTCTCCTTGACAAACAGG NGG 2 0.275 Tier II
18 NC_000071.6 5 64110312 - Pgm2 NNNTCTGCTTTACACACAGA NGG 2 0.2557 Tier II
19 NC_000068.7 2 29035182 - Cfap77 NNNTCAGCTTTACACACAGG NGG 2 0.2364 Tier II
20 NC_000075.6 9 108148771 + Bsn NNNCCTGCTTTACACACAGG NGG 2 0.1765 Tier II
21 NC_000073.6 7 134324528 - D7Ertd443e NNNTTTGCTTTACAAACAGG NAG 2 0.165 Tier II
22 NC_000077.6 11 55007777 - Anxa6 NNNACTGCTTTCCAAACAGG NGG 2 0.1253 Tier II
23 NC_000067.6 1 15322758 + Kcnb2 NNNTCTGGTTTACAAGCAGG NGG 2 0.1236 Tier II
24 NC_000079.6 13 54526884 - Simc1 NNNTCTGCTTTACAAATAGG NAG 2 0.121 Tier II
25 NC_000069.6 3 138102723 + Mttp NNNTCTGCTGTACAAGCAGG NGG 2 0.0962 Tier II
26 NC_000072.6 6 80169729 - Lrrtm4 NNNTCTGCTTTACATACAGG NAG 2 0.0519 Tier II
27 NC_000075.6 9 23333252 - Bmper NNNTCTGCTTTACAAACAAG NGA 2 0.0496 Tier II
28 NC_000069.6 3 69460169 + Ppm1l NNNTCTGCTTTAGACACAGG NGG 2 0.0372 Tier II
29 NC_000076.6 10 20627937 - Pde7b NNNTCTGCTCTACAAACAGG NTG 2 0.0208 Tier II
30 NC_000070.6 4 14609427 + Slc26a7 NNNTCTGCTTCACAAACAGG NGC 2 0.0148 Tier II
31 NC_000073.6 7 130660645 + Tacc2 NNNTCTGCTTTAGAAAGAGG NGG 2 0.008 Tier II
32 NC_000067.6 1 159569776 + Tnr NNNTCTGCTTTACAAAGAGG NGT 2 0.0009 Tier II
33 NC_000084.6 18 62099728 - Gm41750 NNNTTTGCTTTATAAACAGG NGG 2 0.4455 Tier III
34 NC_000086.7 X 155646213 - Gm15155 NNNTCTGCTGTACAAACATG NGG 2 0.3333 Tier III
35 NC_000069.6 3 69460169 + Gm52607 NNNTCTGCTTTAGACACAGG NGG 2 0.0372 Tier III
36 NC_000076.6 10 20627937 - Gm17230 NNNTCTGCTCTACAAACAGG NTG 2 0.0208 Tier III
37 NC_000070.6 4 129384568 + C330020E22Rik NNNTCTGCTTTACAACCAGC NGG 2 0.0 Tier III
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Other clones with same target sequence:

(none)