Construct: sgRNA BRDN0001146668

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
AGCCGGAAGAAGGACTGGGT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
UCKL1 (54963)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76345

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000020.11 20 63945806 - UCKL1 NNNCGGAAGAAGGACTGGGT NGG 0 1.0 Tier I
2 NC_000006.12 6 30189513 - TRIM26 NNNCGGAAGAAGTTCTGGGT NGG 2 0.16 Tier I
3 NC_000009.12 9 127944884 - EEIG1 NNNCGGAAGAAGGACTCGGT NGA 2 0.0163 Tier I
4 NC_000015.10 15 38034967 - TMCO5A NNNAGGAAGAAGGACTGTGT NGG 2 0.5614 Tier II
5 NC_000001.11 1 50574370 + FAF1 NNNTGGAAGAGGGACTGGGT NGG 2 0.52 Tier II
6 NC_000014.9 14 24405529 + NYNRIN NNNAGGAAGAAGGTCTGGGT NGG 2 0.4491 Tier II
7 NC_000019.10 19 33441545 - PEPD NNNGGGAAGGAGGACTGGGT NGG 2 0.2778 Tier II
8 NC_000002.12 2 237728813 - LRRFIP1 NNNGGGAAGAAGGTCTGGGT NGG 2 0.2667 Tier II
9 NC_000001.11 1 151709403 - CELF3 NNNCGGAAGAAGGGCTGGGA NGG 2 0.2625 Tier II
10 NC_000001.11 1 68172997 + WLS NNNCGGAAGACGGACTGGGA NGG 2 0.225 Tier II
11 NC_000001.11 1 53672132 - GLIS1 NNNCGGAAGAATGGCTGGGT NGG 2 0.1795 Tier II
12 NC_000011.10 11 72580556 + PDE2A NNNGGGAAGAAGGACGGGGT NGG 2 0.1731 Tier II
13 NC_000001.11 1 15445529 - CTRC NNNGGGAAGCAGGACTGGGT NGG 2 0.1667 Tier II
14 NC_000023.11 X 9804030 + SHROOM2 NNNGGGAAGATGGACTGGGT NGG 2 0.1538 Tier II
15 NC_000003.12 3 49889160 - MST1R NNNGGGAAGAAGGACTGGGC NGG 2 0.0455 Tier II
16 NC_000007.14 7 51026500 + COBL NNNCGGAAGAAGGGGTGGGT NGG 2 0.0233 Tier II
17 NC_000012.12 12 10855083 - PRH1-PRR4 NNNCAGGAGAAGGACTGGGT NGG 2 0.6118 Tier III
18 NC_000012.12 12 116487232 + LOC105370007 NNNAGGAAGGAGGACTGGGT NGG 2 0.4678 Tier III
19 NC_000004.12 4 9686837 + LOC124900660 NNNCGGAAAAAGGACTGAGT NGG 2 0.4451 Tier III
20 NC_000001.11 1 38092761 + MIR3659HG NNNGGGAGGAAGGACTGGGT NGG 2 0.3667 Tier III
21 NC_000022.11 22 18064703 + LOC105372853 NNNGGGAATAAGGACTGGGT NGG 2 0.2667 Tier III
22 NC_000001.11 1 68172997 + GNG12-AS1 NNNCGGAAGACGGACTGGGA NGG 2 0.225 Tier III
23 NC_000011.10 11 57211380 + LOC105369309 NNNCAGAAGAAGGCCTGGGT NGG 2 0.1857 Tier III
24 NC_000011.10 11 25555753 + LINC02699 NNNCGGAAGAAGGACCTGGT NGG 2 0.1667 Tier III
Download CSV

Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000083.6 17 24628943 - Tsc2 NNNTGGAAGAAGAACTGGGT NGG 2 0.7385 Tier I
2 NC_000068.7 2 181574370 - Uckl1 NNNCGGAAAAAGGACTGGGT NGG 1 0.6429 Tier I
3 NC_000082.6 16 46045290 - Cd96 NNNAGGAAGAAGGACTAGGT NGG 2 0.786 Tier II
4 NC_000087.7 Y 2123786 + Zfy2 NNNAGGAAGTAGGACTGGGT NGG 2 0.743 Tier II
5 NC_000076.6 10 89458434 + Nr1h4 NNNCAGAAGAAGGACTGGTT NGG 2 0.5778 Tier II
6 NC_000084.6 18 5674777 - Zeb1 NNNAGGAAGAAGGACTGTGT NGG 2 0.5614 Tier II
7 NC_000073.6 7 56899257 + Gabrg3 NNNAGGAAGAGGGACTGGGT NGG 2 0.5474 Tier II
8 NC_000081.6 15 78953402 + Triobp NNNCGGAAGGAGAACTGGGT NGG 2 0.5128 Tier II
9 NC_000085.6 19 46268659 + Gbf1 NNNCAGAAGAAGGACTGGGA NGG 2 0.4875 Tier II
10 NC_000073.6 7 47054370 + Tmem86a NNNTGGTAGAAGGACTGGGT NGG 2 0.35 Tier II
11 NC_000082.6 16 8411906 - Tmem114 NNNAGGAAGAAGGACGGGGT NGG 2 0.2915 Tier II
12 NC_000067.6 1 175992389 + Pld5 NNNAGGAAGCAGGACTGGGT NGG 2 0.2807 Tier II
13 NC_000068.7 2 141846066 + Macrod2 NNNAGGAAGCAGGACTGGGT NGG 2 0.2807 Tier II
14 NC_000069.6 3 145446856 - Col24a1 NNNAGGAAGCAGGACTGGGT NGG 2 0.2807 Tier II
15 NC_000072.6 6 106479301 + Cntn4 NNNAGGAAGCAGGACTGGGT NGG 2 0.2807 Tier II
16 NC_000073.6 7 57493407 - Gabra5 NNNAGGAAGCAGGACTGGGT NGG 2 0.2807 Tier II
17 NC_000076.6 10 53684803 - Fam184a NNNAGGAAGCAGGACTGGGT NGG 2 0.2807 Tier II
18 NC_000078.6 12 37998187 - Dgkb NNNAGGAAGCAGGACTGGGT NGG 2 0.2807 Tier II
19 NC_000078.6 12 41697684 - Immp2l NNNAGGAAGCAGGACTGGGT NGG 2 0.2807 Tier II
20 NC_000079.6 13 9737210 - Zmynd11 NNNAGGAAGCAGGACTGGGT NGG 2 0.2807 Tier II
21 NC_000079.6 13 62989479 + Aopep NNNAGGAAGCAGGACTGGGT NGG 2 0.2807 Tier II
22 NC_000079.6 13 63007918 + Aopep NNNAGGAAGCAGGACTGGGT NGG 2 0.2807 Tier II
23 NC_000080.6 14 4823977 + Gm26650 NNNAGGAAGCAGGACTGGGT NGG 2 0.2807 Tier II
24 NC_000081.6 15 30535886 - Ctnnd2 NNNAGGAAGCAGGACTGGGT NGG 2 0.2807 Tier II
25 NC_000083.6 17 42443909 - Ptchd4 NNNAGGAAGCAGGACTGGGT NGG 2 0.2807 Tier II
26 NC_000086.7 X 42163376 - Stag2 NNNAGGAAGCAGGACTGGGT NGG 2 0.2807 Tier II
27 NC_000086.7 X 84170167 + Dmd NNNAGGAAGCAGGACTGGGT NGG 2 0.2807 Tier II
28 NC_000074.6 8 25536235 - Fgfr1 NNNCGGGAGAAGGACGGGGT NGG 2 0.2443 Tier II
29 NC_000069.6 3 102826275 + Sycp1 NNNGGGAAGCAGGACTGGGT NGG 2 0.1667 Tier II
30 NC_000078.6 12 99394624 - Foxn3 NNNAGGAAGAAGGACTGGGG NGG 2 0.1486 Tier II
31 NC_000074.6 8 75216506 + Rasd2 NNNCTGCAGAAGGACTGGGT NGG 2 0.1313 Tier II
32 NC_000077.6 11 5151487 - Emid1 NNNCGGAGGAAGGACTGGGG NGG 2 0.1294 Tier II
33 NC_000071.6 5 124829511 - Dnah10 NNNCTGAAGAAGGACTGGGT NAG 2 0.0778 Tier II
34 NC_000072.6 6 90334291 + Uroc1 NNNCGGTAGAAGGACTGGGG NGG 2 0.0772 Tier II
35 NC_000075.6 9 123462675 - Lars2 NNNAGGAAGAAGGACTGGGT NGA 2 0.0585 Tier II
36 NC_000086.7 X 161146286 - Scml2 NNNAGGAAGAAGGATTGGGT NGG 2 0.0561 Tier II
37 NC_000067.6 1 16480463 + Stau2 NNNAGGAAGAAGGAGTGGGT NGG 2 0.0421 Tier II
38 NC_000068.7 2 105278913 + Them7 NNNAGGAAGAAGGAGTGGGT NGG 2 0.0421 Tier II
39 NC_000069.6 3 102850261 + Sycp1 NNNAGGAAGAAGGAGTGGGT NGG 2 0.0421 Tier II
40 NC_000074.6 8 11225267 - Col4a1 NNNAGGAAGAAGGAGTGGGT NGG 2 0.0421 Tier II
41 NC_000074.6 8 129052628 - Ccdc7a NNNAGGAAGAAGGAGTGGGT NGG 2 0.0421 Tier II
42 NC_000075.6 9 4775579 + Gria4 NNNAGGAAGAAGGAGTGGGT NGG 2 0.0421 Tier II
43 NC_000075.6 9 24736764 + Tbx20 NNNAGGAAGAAGGAGTGGGT NGG 2 0.0421 Tier II
44 NC_000076.6 10 27349572 + Lama2 NNNAGGAAGAAGGAGTGGGT NGG 2 0.0421 Tier II
45 NC_000080.6 14 4815834 - Gm26650 NNNAGGAAGAAGGAGTGGGT NGG 2 0.0421 Tier II
46 NC_000080.6 14 124451699 - Fgf14 NNNAGGAAGAAGGAGTGGGT NGG 2 0.0421 Tier II
47 NC_000082.6 16 74382933 - Robo2 NNNAGGAAGAAGGAGTGGGT NGG 2 0.0421 Tier II
48 NC_000085.6 19 57753842 - Atrnl1 NNNAGGAAGAAGGAGTGGGT NGG 2 0.0421 Tier II
49 NC_000086.7 X 20929150 + Cfp NNNAGGAAGAAGGAGTGGGT NGG 2 0.0421 Tier II
50 NC_000086.7 X 95546005 - Asb12 NNNAGGAAGAAGGAGTGGGT NGG 2 0.0421 Tier II
51 NC_000086.7 X 137701264 - Il1rapl2 NNNAGGAAGAAGGAGTGGGT NGG 2 0.0421 Tier II
52 NC_000075.6 9 92542787 - Plod2 NNNCGGAAGGAGGAGTGGGT NGG 2 0.0278 Tier II
53 NC_000080.6 14 41002569 - Prxl2a NNNCTGAAGAAGGACTGGGC NGG 2 0.0273 Tier II
54 NC_000068.7 2 77722843 + Zfp385b NNNGGGAAGAAGGAGTGGGT NGG 2 0.025 Tier II
55 NC_000083.6 17 14284945 - Smoc2 NNNCGGAAGAAGGATTGGGT NAG 2 0.0173 Tier II
56 NC_000072.6 6 40746769 - Mgam NNNCGGAAGCAGGAGTGGGT NGG 2 0.0167 Tier II
57 NC_000083.6 17 57472513 + Adgre1 NNNCGGAAGCAGGAGTGGGT NGG 2 0.0167 Tier II
58 NC_000072.6 6 15145921 - Foxp2 NNNCGGAAGAAGGAGTGGGT NGA 2 0.0035 Tier II
59 NC_000067.6 1 80463792 - 1700016L21Rik NNNAGGAAGAGGGACTGGGT NGG 2 0.5474 Tier III
60 NC_000067.6 1 80463792 - Gm38673 NNNAGGAAGAGGGACTGGGT NGG 2 0.5474 Tier III
61 NC_000074.6 8 50250246 - 1700019L22Rik NNNGGGAAGAAGGACTGGGA NGG 2 0.2813 Tier III
62 NC_000076.6 10 57148388 + Gm40652 NNNAGGAAGCAGGACTGGGT NGG 2 0.2807 Tier III
63 NC_000078.6 12 15035190 - 4930448C13Rik NNNAGGAAGCAGGACTGGGT NGG 2 0.2807 Tier III
64 NC_000078.6 12 86236374 - Gm40481 NNNAGGAAGCAGGACTGGGT NGG 2 0.2807 Tier III
65 NC_000080.6 14 6458673 - Gm26630 NNNAGGAAGCAGGACTGGGT NGG 2 0.2807 Tier III
66 NC_000080.6 14 6793776 - Gm26680 NNNAGGAAGCAGGACTGGGT NGG 2 0.2807 Tier III
67 NC_000080.6 14 6926260 - Gm32041 NNNAGGAAGCAGGACTGGGT NGG 2 0.2807 Tier III
68 NC_000078.6 12 60200107 - Gm40431 NNNGGGAAGGAGGACTGGGT NGG 2 0.2778 Tier III
69 NC_000074.6 8 25536235 - Gm31288 NNNCGGGAGAAGGACGGGGT NGG 2 0.2443 Tier III
70 NC_000080.6 14 6780672 + Gm26680 NNNAGGAAGAAGGAATGGGT NGG 2 0.1871 Tier III
71 NC_000080.6 14 6913735 + Gm32041 NNNAGGAAGAAGGAATGGGT NGG 2 0.1871 Tier III
72 NC_000072.6 6 41348754 + Tcrb NNNGGGAAGCAGGACTGGGT NGG 2 0.1667 Tier III
73 NC_000073.6 7 94911097 + Gm32647 NNNAGGAAGAAGGAGTGGGT NGG 2 0.0421 Tier III
74 NC_000075.6 9 72232321 + Gm27188 NNNAGGAAGAAGGAGTGGGT NGG 2 0.0421 Tier III
75 NC_000077.6 11 41899198 - A230004M16Rik NNNAGGAAGAAGGAGTGGGT NGG 2 0.0421 Tier III
76 NC_000078.6 12 14238534 - Gm36007 NNNAGGAAGAAGGAGTGGGT NGG 2 0.0421 Tier III
77 NC_000080.6 14 6466780 + Gm26630 NNNAGGAAGAAGGAGTGGGT NGG 2 0.0421 Tier III
78 NC_000080.6 14 6934312 + Gm32041 NNNAGGAAGAAGGAGTGGGT NGG 2 0.0421 Tier III
79 NC_000085.6 19 8540213 - Gm8189 NNNAGGAAGAAGGAGTGGGT NGG 2 0.0421 Tier III
80 NC_000075.6 9 92542787 - B430319G15Rik NNNCGGAAGGAGGAGTGGGT NGG 2 0.0278 Tier III
81 NC_000069.6 3 106864830 - Gm35507 NNNGGGAAGAAGGAGTGGGT NGG 2 0.025 Tier III
82 NC_000080.6 14 53735268 - Tcra NNNCGGAAGCAGGAGTGGGT NGG 2 0.0167 Tier III
Download CSV

Other clones with same target sequence:

(none)