Construct: sgRNA BRDN0001146689

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
GAAGTGCAAACACTTGCTTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PRKD3 (23683)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
75951

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000002.12 2 37274461 + PRKD3 NNNGTGCAAACACTTGCTTG NGG 0 1.0 Tier I
2 NC_000010.11 10 80634417 + SH2D4B NNNGTTCAAACACTTTCTTG NGG 2 0.0 Tier I
3 NC_000017.11 17 48396113 - SKAP1 NNNATGCAAACACTTGCTTG NGG 1 0.9 Tier II
4 NC_000007.14 7 30870615 - MINDY4 NNNGGGAAAACACTTGCTTG NGG 2 0.48 Tier II
5 NC_000020.11 20 54575802 - DOK5 NNNGTGCACACACTTGCTTT NGG 2 0.4 Tier II
6 NC_000003.12 3 71301029 - FOXP1 NNNCTGCATACACTTGCTTG NGG 2 0.3176 Tier II
7 NC_000006.12 6 7141760 + RREB1 NNNGTGCAGACACTTGCCTG NGG 2 0.2857 Tier II
8 NC_000009.12 9 135941712 + UBAC1 NNNGGGCAAAGACTTGCTTG NGG 2 0.16 Tier II
9 NC_000012.12 12 109948693 - GIT2 NNNGTGCAAACAGTTGCTTG NGG 1 0.1364 Tier II
10 NC_000017.11 17 4501518 + SPNS2 NNNGTGCAAACACTTGCCTG NGC 2 0.0095 Tier II
11 NC_000022.11 22 44995084 + PHF21B NNNGTGCAAACACTGCCTTG NGG 2 0.0 Tier II
12 NC_000023.11 X 132220726 - RAP2C-AS1 NNNGTGAAATCACTTGCTTG NGG 2 0.6618 Tier III
13 NC_000007.14 7 30870615 - INMT-MINDY4 NNNGGGAAAACACTTGCTTG NGG 2 0.48 Tier III
14 NC_000003.12 3 71301029 - FOXP1-AS1 NNNCTGCATACACTTGCTTG NGG 2 0.3176 Tier III
15 NC_000015.10 15 45802505 + LOC105370802 NNNGTGCAAATATTTGCTTG NGG 2 0.2154 Tier III
16 NC_000003.12 3 8306343 - LMCD1-AS1 NNNGTGCAAACACATGCTAG NGG 2 0.1769 Tier III
17 NC_000005.10 5 109573801 - LOC105379117 NNNGTGCAAACACCTGTTTG NGG 2 0.1333 Tier III
18 NC_000002.12 2 121723250 - NIFK-AS1 NNNGTGAAAACAGTTGCTTG NGG 2 0.1023 Tier III
19 NC_000012.12 12 42652373 + LINC02451 NNNGTGCAATCACTTGCTTG NCG 2 0.0945 Tier III
20 NC_000003.12 3 167099339 + LOC105374196 NNNGTGCAAACAGTTGTTTG NGG 2 0.0636 Tier III
21 NC_000001.11 1 219548860 - LYPLAL1-AS1 NNNGTGCAAGCACTTGCTTG NTG 2 0.0216 Tier III
22 NC_000022.11 22 27678015 + CPMER NNNGTGCAAACACTTGCCTG NTG 2 0.0167 Tier III
23 NC_000010.11 10 80634417 + LOC105378387 NNNGTTCAAACACTTTCTTG NGG 2 0.0 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000083.6 17 78966215 + Prkd3 NNNGTGCAAACACTTGCTTG NGG 0 1.0 Tier I
2 NC_000076.6 10 73649090 - Pcdh15 NNNGTATAAACACTTGCTTG NGG 2 0.8125 Tier II
3 NC_000072.6 6 90904952 - Iqsec1 NNNGTGTACACACTTGCTTG NGG 2 0.4643 Tier II
4 NC_000070.6 4 48069412 + Nr4a3 NNNGTGCAGACACTAGCTTG NGG 2 0.386 Tier II
5 NC_000071.6 5 21209866 - Gsap NNNGTTCAAACACTTGTTTG NGG 2 0.3111 Tier II
6 NC_000072.6 6 121454093 + Iqsec3 NNNGTGCAAACACTTGCTTA NAG 2 0.2431 Tier II
7 NC_000076.6 10 13327965 + Phactr2 NNNTGGCAAACACTTGCTTG NGG 2 0.2327 Tier II
8 NC_000074.6 8 12633146 - Tubgcp3 NNNGAGCCAACACTTGCTTG NGG 2 0.2143 Tier II
9 NC_000077.6 11 63069530 - Tekt3 NNNGTGCAAACACATGCGTG NGG 2 0.2063 Tier II
10 NC_000071.6 5 148241609 - Mtus2 NNNTAGCAAACACTTGCTTG NGG 2 0.1818 Tier II
11 NC_000071.6 5 45574837 + Fam184b NNNGTGCAAAGACTTGCATG NGG 2 0.1667 Tier II
12 NC_000074.6 8 119717676 + Atp2c2 NNNGTGCTAACTCTTGCTTG NGG 2 0.1429 Tier II
13 NC_000085.6 19 10098122 + Fads2 NNNGTGCAAACACTGGCTTG NGG 1 0.05 Tier II
14 NC_000073.6 7 38224851 + Plekhf1 NNNATGCAAACACTTGCTTG NGC 2 0.02 Tier II
15 NC_000072.6 6 106454628 - Cntn4 NNNGTGCAAACACTTGCATG NGT 2 0.0108 Tier II
16 NC_000068.7 2 148686976 - Gzf1 NNNATGCAAACACTTCCTTG NGG 2 0.0 Tier II
17 NC_000085.6 19 32624783 + Papss2 NNNGTGCAAACACTTTTTTG NGG 2 0.0 Tier II
18 NC_000074.6 8 23869289 + Zmat4 NNNGTGCAAACATGTGCTTG NGG 2 0.0 Tier II
19 NC_000080.6 14 25146449 - Gm32224 NNNGTGCATACACTTGCTTT NGG 2 0.42 Tier III
20 NC_000068.7 2 71602813 - Gm36857 NNNGTGGAAACACTTGCCTG NGG 2 0.2017 Tier III
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Other clones with same target sequence:

(none)