Construct: sgRNA BRDN0001146709
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AAGTGACGGTGTCATGCGGG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- NO_SITE control
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 80240
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000019.10 |
19 |
51694500 |
- |
SPACA6 |
NNNTGAAGGTGTCATGCAGG |
NGG |
2 |
0.5192 |
Tier I |
| 2 |
NC_000020.11 |
20 |
53161709 |
- |
TSHZ2 |
NNNTGATGGTATCATGCGGG |
NGG |
2 |
0.8125 |
Tier II |
| 3 |
NC_000005.10 |
5 |
37711752 |
+ |
WDR70 |
NNNTGATGGTGTCATGTGGG |
NGG |
2 |
0.3792 |
Tier II |
| 4 |
NC_000013.11 |
13 |
112501766 |
- |
TUBGCP3 |
NNNTGAAGGTGTCATGCCGG |
NGG |
2 |
0.3571 |
Tier II |
| 5 |
NC_000001.11 |
1 |
211050542 |
- |
KCNH1 |
NNNTGACGGTGTCATGTGGG |
NTG |
2 |
0.0182 |
Tier II |
| 6 |
NC_000004.12 |
4 |
14136749 |
- |
LINC01085 |
NNNTGACAGTGTCATGCGGA |
NGG |
2 |
0.9375 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000078.6 |
12 |
111039977 |
+ |
Rcor1 |
NNNTGGCGGTGGCATGCGGG |
NGG |
2 |
0.3571 |
Tier I |
| 2 |
NC_000082.6 |
16 |
3957315 |
- |
Nlrc3 |
NNNTGACGGTGTCATGTGCG |
NGG |
2 |
0.2092 |
Tier I |
| 3 |
NC_000070.6 |
4 |
138012342 |
+ |
Eif4g3 |
NNNTGACGGTGTCATGCGAG |
NGG |
1 |
0.7143 |
Tier II |
| 4 |
NC_000084.6 |
18 |
68057875 |
+ |
Ldlrad4 |
NNNTGACGGGGTCATGCGGA |
NGG |
2 |
0.4688 |
Tier II |
| 5 |
NC_000073.6 |
7 |
107622063 |
+ |
Ppfibp2 |
NNNTGAAGGTGTCATGGGGG |
NGG |
2 |
0.0441 |
Tier II |
| 6 |
NC_000068.7 |
2 |
29014391 |
- |
Cfap77 |
NNNTGACGGTGGCATGGGGG |
NGG |
2 |
0.0294 |
Tier II |
| 7 |
NC_000076.6 |
10 |
81513662 |
+ |
Gna15 |
NNNTGACGGTGTCATGGGGC |
NGG |
2 |
0.0252 |
Tier II |
| 8 |
NC_000073.6 |
7 |
84257958 |
- |
Arnt2 |
NNNTGACGGTGTCATGTGGG |
NGT |
2 |
0.0075 |
Tier II |
| 9 |
NC_000073.6 |
7 |
84257958 |
- |
Gm26708 |
NNNTGACGGTGTCATGTGGG |
NGT |
2 |
0.0075 |
Tier III |
| 10 |
NC_000073.6 |
7 |
84257958 |
- |
LOC117997403 |
NNNTGACGGTGTCATGTGGG |
NGT |
2 |
0.0075 |
Tier III |
Other clones with same target sequence:
(none)
