Construct: sgRNA BRDN0001146713
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GCAGCACCTGAGTTAATACA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- MELK (9833)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75669
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000009.12 |
9 |
36599459 |
+ |
MELK |
NNNGCACCTGAGTTAATACA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000016.10 |
16 |
88214165 |
+ |
ZNF469 |
NNNGCACATGAATTAATACA |
NGG |
2 |
0.6067 |
Tier II |
| 3 |
NC_000004.12 |
4 |
88557414 |
- |
HERC3 |
NNNAAACCTGAGTTAATACA |
NGG |
2 |
0.5143 |
Tier II |
| 4 |
NC_000014.9 |
14 |
61470432 |
- |
PRKCH |
NNNGCACATGGGTTAATACA |
NGG |
2 |
0.4225 |
Tier II |
| 5 |
NC_000015.10 |
15 |
100189481 |
- |
ADAMTS17 |
NNNGCACCTGAATTAATAAA |
NGG |
2 |
0.4 |
Tier II |
| 6 |
NC_000001.11 |
1 |
180837867 |
+ |
XPR1 |
NNNGCAACTGATTTAATACA |
NGG |
2 |
0.2885 |
Tier II |
| 7 |
NC_000011.10 |
11 |
21069481 |
+ |
NELL1 |
NNNGCACCTGACTTAATAGA |
NGG |
2 |
0.0662 |
Tier II |
| 8 |
NC_000006.12 |
6 |
158442107 |
- |
TULP4 |
NNNGGACCTGAGTTAATACA |
NGA |
2 |
0.0417 |
Tier II |
| 9 |
NC_000017.11 |
17 |
69321633 |
+ |
ABCA5 |
NNNGCAGCTGAGTTAATACA |
NGA |
2 |
0.0327 |
Tier II |
| 10 |
NC_000010.11 |
10 |
26977724 |
+ |
ANKRD26 |
NNNGCACCTGATTTAATACA |
NGA |
2 |
0.0267 |
Tier II |
| 11 |
NC_000002.12 |
2 |
188233473 |
+ |
LINC01090 |
NNNATACCTGAGTTAATACA |
NGG |
2 |
0.5727 |
Tier III |
| 12 |
NC_000009.12 |
9 |
104591816 |
+ |
LOC107987105 |
NNNGTACCTGATTTAATACA |
NGG |
2 |
0.2448 |
Tier III |
| 13 |
NC_000020.11 |
20 |
5470419 |
- |
LOC107985411 |
NNNGCACCTGAGTGTATACA |
NGG |
2 |
0.0 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000085.6 |
19 |
48282322 |
+ |
Sorcs3 |
NNNGCAGCTGAGTTAATACA |
NGG |
1 |
0.4706 |
Tier II |
| 2 |
NC_000082.6 |
16 |
14003908 |
- |
Bmerb1 |
NNNGCACCTGTGTTAACACA |
NGG |
2 |
0.2172 |
Tier II |
| 3 |
NC_000079.6 |
13 |
42684132 |
+ |
Phactr1 |
NNNGCACCTGACTTTATACA |
NGG |
2 |
0.1059 |
Tier II |
| 4 |
NC_000079.6 |
13 |
108882689 |
+ |
Pde4d |
NNNGCACCAGAGTTAATACA |
NGT |
2 |
0.015 |
Tier II |
| 5 |
NC_000081.6 |
15 |
40067999 |
- |
Gm33251 |
NNNGCACCTGAGTTTAGACA |
NGG |
2 |
0.0235 |
Tier III |
| 6 |
NC_000070.6 |
4 |
13587280 |
+ |
Gm36438 |
NNNGCACCTGAGTTAATACA |
NAC |
2 |
0.0 |
Tier III |
Other clones with same target sequence:
(none)
