Construct: sgRNA BRDN0001146750
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AGCAGCGGATGTGTTCAGGT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PKMYT1 (9088)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77280
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000016.10 |
16 |
2975320 |
- |
PKMYT1 |
NNNAGCGGATGTGTTCAGGT |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000009.12 |
9 |
131433572 |
+ |
PRRC2B |
NNNAGGGGATGTGTTCAGGG |
NGG |
2 |
0.0882 |
Tier II |
| 3 |
NC_000017.11 |
17 |
9941858 |
- |
GAS7 |
NNNATCGGATGTGTTCAGGG |
NGG |
2 |
0.0529 |
Tier II |
| 4 |
NC_000002.12 |
2 |
143362835 |
- |
ARHGAP15 |
NNNAGCAGATGTGTTCAGGT |
NGC |
2 |
0.0222 |
Tier II |
| 5 |
NC_000010.11 |
10 |
105798079 |
- |
LINC02627 |
NNNAGCGGATGTGTGCAGGT |
NGT |
2 |
0.0008 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000081.6 |
15 |
99510587 |
- |
Faim2 |
NNNAGCGGGTGTGTTCACGT |
NGG |
2 |
0.3175 |
Tier I |
| 2 |
NC_000083.6 |
17 |
23734316 |
+ |
Pkmyt1 |
NNNAGCAGATGTGTTCAGGT |
NAG |
2 |
0.2593 |
Tier I |
| 3 |
NC_000075.6 |
9 |
53600177 |
+ |
Acat1 |
NNNAGCAGATGTGTTCATGT |
NGG |
2 |
0.6667 |
Tier II |
| 4 |
NC_000077.6 |
11 |
57029570 |
- |
Gria1 |
NNNAGCAGATGTGTTCAGGA |
NGG |
2 |
0.5625 |
Tier II |
| 5 |
NC_000067.6 |
1 |
20136196 |
+ |
Pkhd1 |
NNNAGCGGCTGTGTTCAGGT |
NCG |
2 |
0.0612 |
Tier II |
Other clones with same target sequence:
(none)
