Construct: sgRNA BRDN0001146764
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TTACATCTAAAGAGCTAGGG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- STK17B (9262)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76798
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000002.12 |
2 |
196163267 |
- |
STK17B |
NNNCATCTAAAGAGCTAGGG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000020.11 |
20 |
43982496 |
+ |
TOX2 |
NNNCAGCTAAAGAGCTAGTG |
NGG |
2 |
0.381 |
Tier II |
| 3 |
NC_000011.10 |
11 |
8400107 |
- |
STK33 |
NNNCATCAAATGAGCTAGGG |
NGG |
2 |
0.2462 |
Tier II |
| 4 |
NC_000011.10 |
11 |
118613660 |
- |
PHLDB1 |
NNNCAGCTAAAGAGCTTGGG |
NGG |
2 |
0.0762 |
Tier II |
| 5 |
NC_000005.10 |
5 |
170660545 |
+ |
KCNIP1 |
NNNCAGCTAAAGAGCTAGGG |
NGA |
2 |
0.0397 |
Tier II |
| 6 |
NC_000014.9 |
14 |
24922262 |
- |
STXBP6 |
NNNCATCTATAGAGCTAGGG |
NGT |
2 |
0.0142 |
Tier II |
| 7 |
NC_000008.11 |
8 |
140087997 |
- |
TRAPPC9 |
NNNCATCTAAAGAGCTAGGG |
NCT |
2 |
0.0 |
Tier II |
| 8 |
NC_000004.12 |
4 |
41912994 |
- |
LOC105374426 |
NNNCATCTACAGAACTAGGG |
NGG |
2 |
0.25 |
Tier III |
| 9 |
NC_000005.10 |
5 |
170660545 |
+ |
KCNIP1-AS1 |
NNNCAGCTAAAGAGCTAGGG |
NGA |
2 |
0.0397 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000082.6 |
16 |
32808150 |
+ |
Smbd1 |
NNNCAACTAAAGAGCAAGGG |
NGG |
2 |
0.7879 |
Tier II |
| 2 |
NC_000073.6 |
7 |
74436828 |
+ |
Slco3a1 |
NNNTATCTAAAGAGCTAGGA |
NGG |
2 |
0.75 |
Tier II |
| 3 |
NC_000073.6 |
7 |
35249413 |
+ |
Gpatch1 |
NNNAATCTAGAGAGCTAGGG |
NGG |
2 |
0.4678 |
Tier II |
| 4 |
NC_000073.6 |
7 |
35249413 |
+ |
Wdr88 |
NNNAATCTAGAGAGCTAGGG |
NGG |
2 |
0.4678 |
Tier II |
| 5 |
NC_000068.7 |
2 |
181578827 |
+ |
Uckl1 |
NNNCAGCTGAAGAGCTAGGG |
NGG |
2 |
0.381 |
Tier II |
| 6 |
NC_000077.6 |
11 |
81708624 |
+ |
Asic2 |
NNNCATGTAAAGAGCTAGAG |
NGG |
2 |
0.3361 |
Tier II |
| 7 |
NC_000072.6 |
6 |
84670527 |
+ |
Exoc6b |
NNNCATGTAAAGAGCTAAGG |
NGG |
2 |
0.3258 |
Tier II |
| 8 |
NC_000068.7 |
2 |
167645297 |
+ |
Tmem189 |
NNNCATCTACAGAGCAAGGG |
NGG |
2 |
0.303 |
Tier II |
| 9 |
NC_000085.6 |
19 |
48713992 |
+ |
Sorcs3 |
NNNCATCTAAAGATCAAGGG |
NGG |
2 |
0.2424 |
Tier II |
| 10 |
NC_000080.6 |
14 |
56433147 |
- |
Rnf17 |
NNNCCTCTAAAGACCTAGGG |
NGG |
2 |
0.2143 |
Tier II |
| 11 |
NC_000068.7 |
2 |
90946081 |
+ |
Celf1 |
NNNCTTCTCAAGAGCTAGGG |
NGG |
2 |
0.2078 |
Tier II |
| 12 |
NC_000074.6 |
8 |
24977038 |
+ |
Adam9 |
NNNCATCTAAAGACCTGGGG |
NGG |
2 |
0.0756 |
Tier II |
| 13 |
NC_000070.6 |
4 |
125516310 |
- |
Grik3 |
NNNAATCTAAAGAGCTAGGG |
NTG |
2 |
0.0328 |
Tier II |
| 14 |
NC_000078.6 |
12 |
72460433 |
- |
Lrrc9 |
NNNCATCTAAAGAGCTAGCG |
NGT |
2 |
0.0072 |
Tier II |
| 15 |
NC_000068.7 |
2 |
181578827 |
+ |
Uckl1os |
NNNCAGCTGAAGAGCTAGGG |
NGG |
2 |
0.381 |
Tier III |
| 16 |
NC_000072.6 |
6 |
16780634 |
+ |
Gm36669 |
NNNCATGTAAAGAGCTAGTG |
NGG |
2 |
0.3137 |
Tier III |
| 17 |
NC_000081.6 |
15 |
69957230 |
+ |
Gm19782 |
NNNCATTTAAAGAGCTGGGG |
NGG |
2 |
0.1434 |
Tier III |
| 18 |
NC_000068.7 |
2 |
14546086 |
- |
Gm13266 |
NNNCATCTAAAGAGCTAGTG |
NGT |
2 |
0.0108 |
Tier III |
Other clones with same target sequence:
(none)
