Construct: sgRNA BRDN0001146769
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- ATTCCAGATGATTTGACGAA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- MAK (4117)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76710
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000006.12 |
6 |
10796263 |
+ |
MAK |
NNNCCAGATGATTTGACGAA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000011.10 |
11 |
119314540 |
+ |
MCAM |
NNNCCAGATGACTTGAGGAA |
NGG |
2 |
0.0557 |
Tier I |
| 3 |
NC_000001.11 |
1 |
241533293 |
- |
KMO |
NNNCCAAATGATTTGACCAA |
NGG |
2 |
0.4762 |
Tier II |
| 4 |
NC_000003.12 |
3 |
188287520 |
+ |
LPP |
NNNCCAGATGGTTTGACAAA |
NGG |
2 |
0.45 |
Tier II |
| 5 |
NC_000010.11 |
10 |
74861947 |
+ |
KAT6B |
NNNCAAGATGATTTGACGAT |
NGG |
2 |
0.3429 |
Tier II |
| 6 |
NC_000005.10 |
5 |
168765767 |
+ |
SLIT3 |
NNNCCAGAGGATTTGAAGAA |
NGG |
2 |
0.2889 |
Tier II |
| 7 |
NC_000008.11 |
8 |
105761650 |
+ |
ZFPM2 |
NNNCAAGATGATTTGACGCA |
NGG |
2 |
0.1182 |
Tier II |
| 8 |
NC_000001.11 |
1 |
206902315 |
+ |
IL24 |
NNNCAAGATGATTTTACGAA |
NGG |
2 |
0.0816 |
Tier II |
| 9 |
NC_000002.12 |
2 |
132178435 |
+ |
ANKRD30BL |
NNNCCAGATGATTTGACTAA |
NTG |
2 |
0.026 |
Tier II |
| 10 |
NC_000013.11 |
13 |
33038533 |
+ |
KL |
NNNCCAGATGATTTGATGAA |
NTG |
2 |
0.0182 |
Tier II |
| 11 |
NC_000001.11 |
1 |
20643178 |
- |
PINK1 |
NNNCCAGATGGTTGGACGAA |
NGG |
2 |
0.0 |
Tier II |
| 12 |
NC_000011.10 |
11 |
82168318 |
- |
MIR4300HG |
NNNCCAGGTGATTTGACTAA |
NGG |
2 |
0.4889 |
Tier III |
| 13 |
NC_000011.10 |
11 |
82816500 |
- |
LINC02734 |
NNNCCAGATGATTTGATAAA |
NGG |
2 |
0.3231 |
Tier III |
| 14 |
NC_000001.11 |
1 |
20643178 |
- |
PINK1-AS |
NNNCCAGATGGTTGGACGAA |
NGG |
2 |
0.0 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000076.6 |
10 |
51615783 |
+ |
Gprc6a |
NNNCCAGATGATTTCACGAC |
NGG |
2 |
0.062 |
Tier I |
| 2 |
NC_000073.6 |
7 |
65070077 |
- |
Fam189a1 |
NNNCCAGATGATTTGACGAA |
NGG |
0 |
1.0 |
Tier II |
| 3 |
NC_000082.6 |
16 |
24509276 |
+ |
Lpp |
NNNCCAGATGGTTTGACAAA |
NGG |
2 |
0.45 |
Tier II |
| 4 |
NC_000082.6 |
16 |
16337992 |
+ |
Dnm1l |
NNNCCAGAGGATTTGATGAA |
NGG |
2 |
0.2889 |
Tier II |
| 5 |
NC_000081.6 |
15 |
88972540 |
+ |
Mlc1 |
NNNCCAGAGGATTTGAGGAA |
NGG |
2 |
0.0364 |
Tier II |
| 6 |
NC_000082.6 |
16 |
42337087 |
+ |
Gap43 |
NNNCCAGATGATTTGGGGAA |
NGG |
2 |
0.0113 |
Tier II |
| 7 |
NC_000073.6 |
7 |
35823108 |
+ |
E130304I02Rik |
NNNCCATATGATTTGAGGAA |
NGG |
2 |
0.0336 |
Tier III |
Other clones with same target sequence:
(none)
