Construct: sgRNA BRDN0001146795
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CAAGTGAGCAGTCTCCACTA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- ATP23 (91419)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77434
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000012.12 |
12 |
57953626 |
- |
ATP23 |
NNNGTGAGCAGTCTCCACTA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000001.11 |
1 |
198744186 |
+ |
PTPRC |
NNNGTGAGCCGTCTCCACTA |
NAG |
2 |
0.0864 |
Tier I |
| 3 |
NC_000005.10 |
5 |
160676721 |
- |
ATP10B |
NNNGTTAGAAGTCTCCACTA |
NGG |
2 |
0.5714 |
Tier II |
| 4 |
NC_000003.12 |
3 |
39932321 |
- |
MYRIP |
NNNGTGAGCAGTTTCCACTT |
NGG |
2 |
0.42 |
Tier II |
| 5 |
NC_000011.10 |
11 |
46635751 |
- |
ATG13 |
NNNGGGTGCAGTCTCCACTA |
NGG |
2 |
0.28 |
Tier II |
| 6 |
NC_000005.10 |
5 |
72145193 |
- |
MAP1B |
NNNGTGAGCATTTTCCACTA |
NGG |
2 |
0.2692 |
Tier II |
| 7 |
NC_000003.12 |
3 |
136074800 |
- |
PPP2R3A |
NNNGTGAGCAGTCTCCACTA |
NAG |
1 |
0.2593 |
Tier II |
| 8 |
NC_000008.11 |
8 |
1944542 |
+ |
ARHGEF10 |
NNNGTCAGCAGTCTCCACCA |
NGG |
2 |
0.1881 |
Tier II |
| 9 |
NC_000002.12 |
2 |
1351810 |
- |
SNTG2 |
NNNGTGAGCATTCTCCACCA |
NGG |
2 |
0.1061 |
Tier II |
| 10 |
NC_000005.10 |
5 |
151336322 |
- |
SLC36A2 |
NNNGTGAGCAGCCTCCACTA |
NCG |
2 |
0.1015 |
Tier II |
| 11 |
NC_000017.11 |
17 |
79404354 |
+ |
RBFOX3 |
NNNGTGAGCAGTCTACACTC |
NGG |
2 |
0.0505 |
Tier II |
| 12 |
NC_000009.12 |
9 |
96896958 |
- |
ZNF782 |
NNNTTGAGCAGTCTCCACTA |
NGT |
2 |
0.0059 |
Tier II |
| 13 |
NC_000010.11 |
10 |
13778179 |
- |
FRMD4A |
NNNGTGAGCAGTCTCCACCA |
NGT |
2 |
0.0044 |
Tier II |
| 14 |
NC_000001.11 |
1 |
29519193 |
+ |
LOC107984933 |
NNNGTGGGCAGTCCCCACTA |
NGG |
2 |
0.2017 |
Tier III |
| 15 |
NC_000002.12 |
2 |
144831044 |
+ |
TEX41 |
NNNGTGAGCAGTCCCCACAA |
NGG |
2 |
0.0816 |
Tier III |
| 16 |
NC_000003.12 |
3 |
153422367 |
- |
LINC02006 |
NNNGTGAGCAGTCTGCACTT |
NGG |
2 |
0.03 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000068.7 |
2 |
158132487 |
+ |
Tgm2 |
NNNGTGAGCAGTCCCGACTA |
NGG |
2 |
0.044 |
Tier I |
| 2 |
NC_000073.6 |
7 |
121873702 |
- |
Scnn1b |
NNNGTGAGCAGACTCCACTG |
NGG |
2 |
0.6118 |
Tier II |
| 3 |
NC_000069.6 |
3 |
63719925 |
- |
Plch1 |
NNNATGAGCAGTCTCCAATA |
NGG |
2 |
0.4846 |
Tier II |
| 4 |
NC_000080.6 |
14 |
18025830 |
- |
Thrb |
NNNGTGAGCATTCTCCACTA |
NGG |
1 |
0.3846 |
Tier II |
| 5 |
NC_000071.6 |
5 |
103093764 |
- |
Mapk10 |
NNNGTGTGCAGTTTCCACTA |
NGG |
2 |
0.3062 |
Tier II |
| 6 |
NC_000070.6 |
4 |
139800747 |
- |
Pax7 |
NNNGTGAGCGGGCTCCACTA |
NGG |
2 |
0.2778 |
Tier II |
| 7 |
NC_000084.6 |
18 |
35978903 |
+ |
Psd2 |
NNNGCGAGCAGTCTCCACGA |
NGG |
2 |
0.25 |
Tier II |
| 8 |
NC_000069.6 |
3 |
127821534 |
+ |
Ap1ar |
NNNGTCAGCAGTCTACACTA |
NGG |
2 |
0.1515 |
Tier II |
| 9 |
NC_000085.6 |
19 |
28646515 |
+ |
Glis3 |
NNNGTGAGCTGTCTCCTCTA |
NGG |
2 |
0.1176 |
Tier II |
| 10 |
NC_000083.6 |
17 |
25656163 |
+ |
Lmf1 |
NNNGTGGGCAGTGTCCACTA |
NGG |
2 |
0.0963 |
Tier II |
| 11 |
NC_000067.6 |
1 |
78346676 |
- |
Sgpp2 |
NNNGTGAGCTGTCTCCACTA |
NGA |
2 |
0.0613 |
Tier II |
| 12 |
NC_000074.6 |
8 |
25422050 |
- |
Gm39147 |
NNNGTGAGCAGTCTCCAGGA |
NGG |
2 |
0.0333 |
Tier II |
| 13 |
NC_000075.6 |
9 |
68921603 |
- |
Rora |
NNNGTGAGCAGTCTGCAATA |
NGG |
2 |
0.0269 |
Tier II |
| 14 |
NC_000073.6 |
7 |
83725894 |
- |
Il16 |
NNNGTGAGCAGTCTCCACCA |
NGA |
2 |
0.0192 |
Tier II |
| 15 |
NC_000071.6 |
5 |
120811608 |
+ |
Oas1c |
NNNGTGAGCAGTCTCCACTA |
NCA |
2 |
0.0 |
Tier II |
| 16 |
NC_000074.6 |
8 |
25422050 |
- |
Gm30978 |
NNNGTGAGCAGTCTCCAGGA |
NGG |
2 |
0.0333 |
Tier III |
| 17 |
NC_000075.6 |
9 |
68921603 |
- |
Gm31262 |
NNNGTGAGCAGTCTGCAATA |
NGG |
2 |
0.0269 |
Tier III |
| 18 |
NC_000070.6 |
4 |
53361705 |
- |
Gm12496 |
NNNGTGAGCAGTTTCCACTA |
NGT |
2 |
0.0113 |
Tier III |
Other clones with same target sequence:
(none)
