Construct: sgRNA BRDN0001146805

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
AGGACAGGTGGATCAATGCA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MARK3 (4140)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77717

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000014.9 14 103466370 + MARK3 NNNACAGGTGGATCAATGCA NGG 0 1.0 Tier I
2 NC_000011.10 11 12148758 + MICAL2 NNNACAAGTAGATCAATGCA NGG 2 0.9333 Tier II
3 NC_000017.11 17 3955239 - ATP2A3 NNNACAGGTGGACCAATGCA NGG 1 0.7895 Tier II
4 NC_000016.10 16 52529550 + TOX3 NNNACAGGTGAATCAAAGCA NGG 2 0.5333 Tier II
5 NC_000002.12 2 98623245 + MGAT4A NNNACAGGTGGACCAATGCT NGG 2 0.4737 Tier II
6 NC_000001.11 1 64651027 - CACHD1 NNNAGAGGTGGGTCAATGCA NGG 2 0.4333 Tier II
7 NC_000007.14 7 45577358 + ADCY1 NNNACATGGGGATCAATGCA NGG 2 0.3537 Tier II
8 NC_000001.11 1 176677506 + PAPPA2 NNNACAGGTGGATCAATGAG NGG 2 0.3277 Tier II
9 NC_000003.12 3 169195271 + MECOM NNNCCAGGTGGATCAATGCT NGG 2 0.2118 Tier II
10 NC_000003.12 3 129570182 + PLXND1 NNNAGAGGTGGCTCAATGCA NGG 2 0.1579 Tier II
11 NC_000006.12 6 116714407 - KPNA5 NNNAGAGGTGGCTCAATGCA NGG 2 0.1579 Tier II
12 NC_000016.10 16 11460722 - LOC400499 NNNACAGGTGGAGCAAAGCA NGG 2 0.1391 Tier II
13 NC_000004.12 4 186208781 - CYP4V2 NNNACAGGTGGATCAAGGCA NGG 1 0.1176 Tier II
14 NC_000004.12 4 186208045 - CYP4V2 NNNACACGTGGATCAAGGCA NGG 2 0.0809 Tier II
15 NC_000004.12 4 186208280 - CYP4V2 NNNACACGTGGATCAAGGCA NGG 2 0.0809 Tier II
16 NC_000004.12 4 186208725 - CYP4V2 NNNACACGTGGATCAAGGCA NGG 2 0.0809 Tier II
17 NC_000003.12 3 25559816 + RARB NNNATAGGTGGATCAATGGA NGG 2 0.0795 Tier II
18 NC_000004.12 4 186208000 - CYP4V2 NNNACATGTGGATCAAGGCA NGG 2 0.0672 Tier II
19 NC_000004.12 4 186208213 - CYP4V2 NNNACATGTGGATCAAGGCA NGG 2 0.0672 Tier II
20 NC_000004.12 4 186208448 - CYP4V2 NNNACATGTGGATCAAGGCA NGG 2 0.0672 Tier II
21 NC_000015.10 15 74720893 + CYP1A1 NNNACAGGAGGATCAATGCA NTG 2 0.0362 Tier II
22 NC_000004.12 4 1921565 + NSD2 NNNACAGGTGGATCAATGAA NGC 2 0.0095 Tier II
23 NC_000002.12 2 96349858 + NCAPH NNNACAGGTAGATGAATGCA NGG 2 0.0 Tier II
24 NC_000016.10 16 78959517 - WWOX NNNACAGGTGGATGAATGCA NGA 2 0.0 Tier II
25 NC_000009.12 9 134788390 + COL5A1 NNNACAGGTGGATGAATGGA NGG 2 0.0 Tier II
26 NC_000016.10 16 19224527 + SYT17 NNNACAGGTGGATGAATGGA NGG 2 0.0 Tier II
27 NC_000016.10 16 28029619 + GSG1L NNNACAGGTGGATGAATGGA NGG 2 0.0 Tier II
28 NC_000016.10 16 88519822 + ZFPM1 NNNACAGGTGGATGAATGGA NGG 2 0.0 Tier II
29 NC_000016.10 16 88521120 + ZFPM1 NNNACAGGTGGATGGATGCA NGG 2 0.0 Tier II
30 NC_000009.12 9 7304694 + LOC105375970 NNNACAGGTGGATGAATGGA NGG 2 0.0 Tier III
31 NC_000016.10 16 15911411 + LOC107984869 NNNACAGGTGGATGAATGGA NGG 2 0.0 Tier III
32 NC_000016.10 16 88519822 + ZFPM1-AS1 NNNACAGGTGGATGAATGGA NGG 2 0.0 Tier III
33 NC_000016.10 16 88521120 + ZFPM1-AS1 NNNACAGGTGGATGGATGCA NGG 2 0.0 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000079.6 13 38507080 - Txndc5 NNNACAGGTGGATCAGTACA NGG 2 0.1331 Tier I
2 NC_000078.6 12 111627842 + Mark3 NNNACAGGTGGATCAATGCA NGC 1 0.0222 Tier I
3 NC_000075.6 9 123443166 - Lars2 NNNACAGAAGGATCAATGCA NGG 2 0.9286 Tier II
4 NC_000077.6 11 4969336 - Ap1b1 NNNGCAGGAGGATCAATGCA NGG 2 0.5804 Tier II
5 NC_000082.6 16 57678249 + Col8a1 NNNACCAGTGGATCAATGCA NGG 2 0.4545 Tier II
6 NC_000075.6 9 57037985 - Ptpn9 NNNACAGGAGGATCAATGTA NGG 2 0.4286 Tier II
7 NC_000067.6 1 136550493 + Kif14 NNNAAGGGTGGATCAATGCA NGG 2 0.4082 Tier II
8 NC_000076.6 10 12695977 + Utrn NNNACAGATGGATAAATGCA NGG 2 0.35 Tier II
9 NC_000067.6 1 138075939 - Ptprc NNNTCAGGTGGATCAATGCA NAG 2 0.165 Tier II
10 NC_000073.6 7 28076867 - Fcgbp NNNAGAGGTGGATCTATGCA NGG 2 0.12 Tier II
11 NC_000073.6 7 23304947 - Nlrp4e NNNACAGGTAGATCAATGGA NGG 2 0.1167 Tier II
12 NC_000068.7 2 110678464 + Ano3 NNNACAGGTTGATCAATGGA NGG 2 0.1016 Tier II
13 NC_000071.6 5 64100607 - Pgm2 NNNACAGGTGGATCAGTGTA NGG 2 0.0888 Tier II
14 NC_000069.6 3 152644087 - Ak5 NNNACAGGTGGATCTATGCA NAG 2 0.0519 Tier II
15 NC_000083.6 17 6549035 - Gm29721 NNNACAGGTGGATAAATGGA NGG 2 0.0438 Tier II
16 NC_000083.6 17 6702153 + Sytl3 NNNACAGGTGGATAAATGGA NGG 2 0.0438 Tier II
17 NC_000076.6 10 78965679 + Olfr1353 NNNACAGATGGATCAATGCA NGT 2 0.0161 Tier II
18 NC_000068.7 2 121230605 - Trp53bp1 NNNACAGGAGGATCAATGCA NGT 2 0.015 Tier II
19 NC_000068.7 2 155927596 + Uqcc1 NNNACAGGAGGATCAATGCA NGT 2 0.015 Tier II
20 NC_000073.6 7 44059850 + Klk1b21 NNNACAAGTGGATGAATGCA NGG 2 0.0 Tier II
21 NC_000081.6 15 99840676 - Lima1 NNNACAGGAGGATCACTGCA NGG 2 0.0 Tier II
22 NC_000068.7 2 170756650 - Dok5 NNNACAGGTGGATCACTGGA NGG 2 0.0 Tier II
23 NC_000084.6 18 35408813 + Sil1 NNNACAGGTGGATGAAAGCA NGG 2 0.0 Tier II
24 NC_000067.6 1 135402604 - Ipo9 NNNACAGGTGGATGAATGGA NGG 2 0.0 Tier II
25 NC_000071.6 5 36095067 + Sorcs2 NNNACAGGTGGATGAATGGA NGG 2 0.0 Tier II
26 NC_000083.6 17 56954244 + Acer1 NNNACAGGTGGATGAATGGA NGG 2 0.0 Tier II
27 NC_000068.7 2 179561428 - Cdh4 NNNACAGGTGGATGGATGCA NGG 2 0.0 Tier II
28 NC_000073.6 7 119610909 - Gm39079 NNNACACATGGATCAATGCA NGG 2 0.6875 Tier III
29 NC_000068.7 2 60578596 + Gm13566 NNNACAGGTGGCACAATGCA NGG 2 0.1822 Tier III
30 NC_000083.6 17 6382158 - Gm29719 NNNACAGGTGGATAAATGGA NGG 2 0.0438 Tier III
31 NC_000070.6 4 152853775 - Gm30911 NNNACAGGTGGATGAATGGA NGG 2 0.0 Tier III
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Other clones with same target sequence:

(none)