Construct: sgRNA BRDN0001146824
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CTTGAGCTCGATGGGCAACA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- CDK11A (728642)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75890
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000001.11 |
1 |
1707459 |
+ |
CDK11A |
NNNGAGCTCGATGGGCAACA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000001.11 |
1 |
1640327 |
+ |
CDK11B |
NNNGAGCTCGATGGGCGACA |
NGG |
1 |
0.1765 |
Tier I |
| 3 |
NC_000001.11 |
1 |
233133103 |
- |
PCNX2 |
NNNGAGCAGGATGGGCAACA |
NGG |
2 |
0.4952 |
Tier II |
| 4 |
NC_000006.12 |
6 |
126158918 |
- |
TRMT11 |
NNNGAGCTGGATGGGCAACG |
NGG |
2 |
0.4734 |
Tier II |
| 5 |
NC_000004.12 |
4 |
109659862 |
- |
MCUB |
NNNGAGCTCTCTGGGCAACA |
NGG |
2 |
0.325 |
Tier II |
| 6 |
NC_000003.12 |
3 |
127657472 |
- |
PODXL2 |
NNNGAGCTAGATGGCCAACA |
NGG |
2 |
0.2338 |
Tier II |
| 7 |
NC_000002.12 |
2 |
232902807 |
+ |
NGEF |
NNNGAGTTCGATGGCCAACA |
NGG |
2 |
0.2216 |
Tier II |
| 8 |
NC_000020.11 |
20 |
62819896 |
- |
COL9A3 |
NNNGAGCTCGAGGGGCCACA |
NGG |
2 |
0.0882 |
Tier II |
| 9 |
NC_000010.11 |
10 |
51189499 |
- |
PRKG1 |
NNNGAGCTCCATGGGCCACA |
NGG |
2 |
0.0706 |
Tier II |
| 10 |
NC_000007.14 |
7 |
42006175 |
+ |
GLI3 |
NNNGAGCTCGAAGGGCAACA |
NTG |
2 |
0.0312 |
Tier II |
| 11 |
NC_000008.11 |
8 |
102903729 |
+ |
MAILR |
NNNTAGCTCAATGGGCAACA |
NGG |
2 |
0.3394 |
Tier III |
| 12 |
NC_000016.10 |
16 |
27269998 |
+ |
NSMCE1-DT |
NNNGAGCTGGATGGTCAACA |
NGG |
2 |
0.0884 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000068.7 |
2 |
173210670 |
+ |
Zbp1 |
NNNGAGCTCCGTGGGCAACA |
NGG |
2 |
0.26 |
Tier I |
| 2 |
NC_000075.6 |
9 |
15719745 |
+ |
Slc36a4 |
NNNCAGCTCGATGGGCAGCA |
NGG |
2 |
0.2118 |
Tier I |
| 3 |
NC_000077.6 |
11 |
94964175 |
- |
Sgca |
NNNGAGCTTGATGGGCCACA |
NGG |
2 |
0.1544 |
Tier II |
Other clones with same target sequence:
(none)
